ISPye13

  • Family IS3
  • Group IS3
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
CP020442 ND Paracoccus yeei
Paracoccus yeei
Paracoccus yeei FDAARGOS_252
DNA section
IS Length : 1231 bp

Ends


IR Length : 27/27

IRL : TGGACCTGTCGCGGTTTCGTTCCGGTCTTCTGAGAGCGATGCTCTCACCA
IRR : TGGACCTGTCGCGGTTTCGTTCCGGTCAGGTGCTCATGTTAAGCGGCCCT

Insertion site


Left flankDirect repeatRight flankDR Length
tcatgccttaCTCttactccggg3

DNA sequence

TGGACCTGTCGCGGTTTCGTTCCGGTCTTCTGAGAGCGATGCTCTCACCAAAGGAGACAGGATATGGCAGCGACACATAGCGCGGAATTCAAGCGGGATG
CGGTTCGCATCGCGCTCGCCAGCGGCCTGACACGGCGTCAGGTTGCGTCTGATCTTGGGATCGGGCTTTCGACGCTCGGCAAATGGATTAGGGCGATTTC
GGATGAGACGAAGGTCCCGGAACGGGATTCCGATCTGCTGCGCGAGAATGAACGGCTGCGCAAAGAGAACCGCATATTGAGGGAGGAGAGGGAGGTTCTA
AAAAAGGCAGCTCAGTTCTTCGCGGCTCAAAAGACGTGAGATTTCAGTTCATTGCCAACCACCGCGGCGACCTGCCTCGCAGTCGCCTGTGCCAGTTGAT
GGGCGTGACGGATCGCGGATTTCGGGCCTGGCGGCGCAGGCCCCCGTCCATCCGTCAGCGGCGCGATATGGTGATCCTGGCCCATATCCGTGAACAGCAT
CGGTTGAGCCTGGGCAGCTATGGCAGGCCGTGTATGACCGAGGAGCTGAACGAATTGGGCCTGCGGGTCGGACAGCGCCGGGTAGGCCGTCTGATGCGCC
AGAATGGCATTCGCATCATCCGCAGCCGGAAGTTCAAGCGCACCACCGACAGCGATCACGCCTTCAACATCGCGCCGAACCTCTTGCAACAGGACTTTTC
GGCGAGTGCCCCGAACCAGAAGTGGGCGGGCGACATCACCTACATCTGGACGCGTGAGGGCTGGGTTTATCTGGCCGTCATCATCGACCTGTTCTCGAGG
CGCGTGATCGGGTGGGCCATCAGCAACCGGATGAAGCAGGATCTGGCGATCAGGGCGCTGAACATGGCCGTTGCCCTGCGCCGCCCGCCACCCGGCTGTA
TTCACCATACGGATCGCGGCGCGCAATACTGCGCCCATGACTACCAGAAGCTTCTGCGCAAAAACGGGTTCAAAGTATCAATGAGCGGCAAGGGCAATTG
CTATGATAATTCGGCCGTCGAAAGCTTCTTCAAGTCGCTGAAGGCAGAATTGGTCTGGAGGCGCGACTGGCCCACCCGGCGTGATGTCGAGATTGCCCTC
TTCGAATACATCAACGGCTTCTATAACCCACGCCGCAAACACTCAGCCTTGGGCTGGAAATCACCCGTGGCCTTCGAACAGAGGGCCGCTTAACATGAGC
ACCTGACCGGAACGAAACCGCGACAGGTCCA
Recoding section
  • Recoding by frameshift
  • Frame -1
  • Type translational
  • Experimentally demonstrated No

Stimulators :

  • Shine-Dalgarno sequence :
  • Secondary structure :

Recoding motif :

Protein section
ORF number : 3

 

ORF 1
LengthBeginEndStrandFusion ORF
276 bp91 aa64339+No
ORF function : Transposase
Description : First part of the transposase

ORF sequence :

MAATHSAEFKRDAVRIALASGLTRRQVASDLGIGLSTLGKWIRAISDETKVPERDSDLLRENERLRKENRILREEREVLKKAAQFFAAQKT

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
942 bp313 aa2521193+No
ORF function : Transposase
Description : Second part of the transposase

ORF sequence :

TAAQREPHIEGGEGGSKKGSSVLRGSKDVRFQFIANHRGDLPRSRLCQLMGVTDRGFRAWRRRPPSIRQRRDMVILAHIREQHRLSLGSYGRPCMTEELN
ELGLRVGQRRVGRLMRQNGIRIIRSRKFKRTTDSDHAFNIAPNLLQQDFSASAPNQKWAGDITYIWTREGWVYLAVIIDLFSRRVIGWAISNRMKQDLAI
RALNMAVALRRPPPGCIHHTDRGAQYCAHDYQKLLRKNGFKVSMSGKGNCYDNSAVESFFKSLKAELVWRRDWPTRRDVEIALFEYINGFYNPRRKHSAL
GWKSPVAFEQRAA

 

Blast result :
ORF 3
LengthBeginEndStrandFusion ORF
1130 bp376 aa641193+Yes
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MAATHSAEFKRDAVRIALASGLTRRQVASDLGIGLSTLGKWIRAISDETKVPERDSDLLRENERLRKENRILREEREVLKKGSSVLRGSKDVRFQFIANH
RGDLPRSRLCQLMGVTDRGFRAWRRRPPSIRQRRDMVILAHIREQHRLSLGSYGRPCMTEELNELGLRVGQRRVGRLMRQNGIRIIRSRKFKRTTDSDHA
FNIAPNLLQQDFSASAPNQKWAGDITYIWTREGWVYLAVIIDLFSRRVIGWAISNRMKQDLAIRALNMAVALRRPPPGCIHHTDRGAQYCAHDYQKLLRK
NGFKVSMSGKGNCYDNSAVESFFKSLKAELVWRRDWPTRRDVEIALFEYINGFYNPRRKHSALGWKSPVAFEQRAA

 

Blast result :
Comments
ISPye13 is 91% aa similar to IS1133.
ISPye13 was identified by in silico nucleotide sequence analysis of Paracoccus yeei strain FDAARGOS_252. The third ORF is the putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References
1] Chmielowska C., Szuplewska M., Bartosik D. (2018) Direct submission.
2] Goldberg,B., Campos,J., Tallon,L., Sadzewicz,L., Sengamalay,N., Ott,S., Godinez,A., Nagaraj,S., Vavikolanu,K., Aluvathingal,J., Nadendla,S. and Sichtig,H. (2017) Direct GenBank submission.