ISPye10
- Family IS5
- Group IS1031
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| CP020442 | ND | Paracoccus yeei | Paracoccus yeei FDAARGOS_252 |
DNA section
IS Length : 931 bp
Ends
IR Length : 12/15
IRL : GAGCCCGCCCCGAAAAGGGTTGAGCGGTTTCAGTGGGTTGTGATTCTGTT
IRR : GAGCCCGACTCAAAATTCCGCTAACGATCCCAGTTCCTTGCGAGGCGTCT
Comments : Insertion within another IS enabled precisely defining ends and DRs.
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| cgtgtcctgc | TTA | ggggtgtgag | 3 |
| gcctttgata | TAA | gagtcttgta | 3 |
| gcgtcgcgcc | TTA | gacctcttcc | 3 |
| tgcccgatct | tta | gaccgaggca | 3 |
| tgacacaacc | tta/taa | ggccgacctt | 3 |
DNA sequence
GAGCCCGCCCCGAAAAGGGTTGAGCGGTTTCAGTGGGTTGTGATTCTGTTCGGTTGCCAAACTGAACGGAGCCACGCATGCCCTGGACTGAAACCGCCCG
CCGGGAGTATCGGCGGGAACTGCCGCGATATGCAAGCGATCTGACTGATCGGGAATGGGCGTTGGTCATGCCCTTCATGCCGTCGCCACGCCGGCTTGGA
CGCCCACGCCGGACCGATTTGCGGGAGGTGATGAACGCGATCCTCTACATCGCGACGACCGGCTGCCAATGGAGGCAACTGCCGAAGGACTTCCCACCCT
GTTCCACCGTGCAGAGGTATTTCTACGACTGGCGCGACAGCGGCCTGCTCGCGTCGATGCGCTTCGAGCTTGCGATGGAGACGCGAGTGCTGGAAGGCCG
CGAGGCCAGCCCGAGCGCGGGGGTGATCGACAGCCAGAGCGTGAAAACCACCGAAAGCGGCGGCGTTTGCGGCTTCGACGCGGGCAAGAAGGTCAAGGGA
CGCAAGCGCCACATCGTCGTTGACACCATCGGCCTGCTGTTCGGGCTGGTGGTTCATGCCGCCGACATTCAGGATCGCGATGGCGCGCCGGCGGTTCTGA
AATCCATCCGTCATCTCTGCCCCTGGCTTCGGCATGTCTTCGCAGACGGAGGATATGCCGGGCCGAAGTTGCGCGGCGCGCTCGACAGAATGGGCACCTG
GACGTTGCAGATCGTCAAACGGTCCGACACCGCGAATGGCTTTGAGGTCCTGCCCCGCAGATGGGTCGTCGAGCGCACCTTTGCCTGGCTCGGTCGCTGC
CGTCGCCTCGGCAAGGATTGGGAGAAATCCACCGCCTCCGCCGAGGCATGGATCAATGTCGCCCATGTCCGCCTTACAACAAGACGCCTCGCAAGGAACT
GGGATCGTTAGCGGAATTTTGAGTCGGGCTC
CCGGGAGTATCGGCGGGAACTGCCGCGATATGCAAGCGATCTGACTGATCGGGAATGGGCGTTGGTCATGCCCTTCATGCCGTCGCCACGCCGGCTTGGA
CGCCCACGCCGGACCGATTTGCGGGAGGTGATGAACGCGATCCTCTACATCGCGACGACCGGCTGCCAATGGAGGCAACTGCCGAAGGACTTCCCACCCT
GTTCCACCGTGCAGAGGTATTTCTACGACTGGCGCGACAGCGGCCTGCTCGCGTCGATGCGCTTCGAGCTTGCGATGGAGACGCGAGTGCTGGAAGGCCG
CGAGGCCAGCCCGAGCGCGGGGGTGATCGACAGCCAGAGCGTGAAAACCACCGAAAGCGGCGGCGTTTGCGGCTTCGACGCGGGCAAGAAGGTCAAGGGA
CGCAAGCGCCACATCGTCGTTGACACCATCGGCCTGCTGTTCGGGCTGGTGGTTCATGCCGCCGACATTCAGGATCGCGATGGCGCGCCGGCGGTTCTGA
AATCCATCCGTCATCTCTGCCCCTGGCTTCGGCATGTCTTCGCAGACGGAGGATATGCCGGGCCGAAGTTGCGCGGCGCGCTCGACAGAATGGGCACCTG
GACGTTGCAGATCGTCAAACGGTCCGACACCGCGAATGGCTTTGAGGTCCTGCCCCGCAGATGGGTCGTCGAGCGCACCTTTGCCTGGCTCGGTCGCTGC
CGTCGCCTCGGCAAGGATTGGGAGAAATCCACCGCCTCCGCCGAGGCATGGATCAATGTCGCCCATGTCCGCCTTACAACAAGACGCCTCGCAAGGAACT
GGGATCGTTAGCGGAATTTTGAGTCGGGCTC
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 834 bp | 277 aa | 78 | 911 | + | No |
Chemistry : DDE
ORF sequence :
MPWTETARREYRRELPRYASDLTDREWALVMPFMPSPRRLGRPRRTDLREVMNAILYIATTGCQWRQLPKDFPPCSTVQRYFYDWRDSGLLASMRFELAM
ETRVLEGREASPSAGVIDSQSVKTTESGGVCGFDAGKKVKGRKRHIVVDTIGLLFGLVVHAADIQDRDGAPAVLKSIRHLCPWLRHVFADGGYAGPKLRG
ALDRMGTWTLQIVKRSDTANGFEVLPRRWVVERTFAWLGRCRRLGKDWEKSTASAEAWINVAHVRLTTRRLARNWDR
ETRVLEGREASPSAGVIDSQSVKTTESGGVCGFDAGKKVKGRKRHIVVDTIGLLFGLVVHAADIQDRDGAPAVLKSIRHLCPWLRHVFADGGYAGPKLRG
ALDRMGTWTLQIVKRSDTANGFEVLPRRWVVERTFAWLGRCRRLGKDWEKSTASAEAWINVAHVRLTTRRLARNWDR
Blast result :
Comments
ISPye10 is 85% aa similar to ISRl2.
ISPye10 was identified by in silico nucleotide sequence analysis of Paracoccus yeei strain FDAARGOS_252 (there are 5 copies od ISPye10 present in the genome).
ISPye10 was identified by in silico nucleotide sequence analysis of Paracoccus yeei strain FDAARGOS_252 (there are 5 copies od ISPye10 present in the genome).
References
1] Chmielowska C., Szuplewska M., Bartosik D. (2018) Direct submission.
2] Goldberg,B., Campos,J., Tallon,L., Sadzewicz,L., Sengamalay,N., Ott,S., Godinez,A., Nagaraj,S., Vavikolanu,K., Aluvathingal,J., Nadendla,S. and Sichtig,H. (2017) Direct GenBank submission.
2] Goldberg,B., Campos,J., Tallon,L., Sadzewicz,L., Sengamalay,N., Ott,S., Godinez,A., Nagaraj,S., Vavikolanu,K., Aluvathingal,J., Nadendla,S. and Sichtig,H. (2017) Direct GenBank submission.