ISPye6
- Family IS3
- Group IS407
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| CP020444 | ND | Paracoccus yeei | Paracoccus yeei Paracoccus yeei FDAARGOS_252 |
DNA section
IS Length : 1217 bp
Ends
IR Length : 19/25
IRL : TGGTCCTCCCCCACTGAAGTGGTCCGCCGTTATGTTTAGGAAACGGAGGA
IRR : TGATCAGCCCCACCTGAGTGGTCCAAGTTAAAAGTTAGTGCATGACGGGC
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| GGTGAGTGATATACGCGG | GT | TGCTTGAGCGGTTCATCC | 2 |
DNA sequence
TGGTCCTCCCCCACTGAAGTGGTCCGCCGTTATGTTTAGGAAACGGAGGATCGAGAATGGCAAACAAACGACCGAAGCCGGAAGAGATTGTCTCGAAGTT
GCGGCAGGTTGAGGTTCTGATGGGGCAAGGCTTGTCCCGTCTGGACGCGATCAGGCGTATCGGCGTTGTCGAACAGACCTATTACCGCTGGCGCAAGCAA
TACGGCGGAATGGGCGTAGACCAGTTGAAAGAGCTGAAGCGCCTTCAGCAGGAGAATGAGCGGCTCAGGCGTGCGGTGTCTGACCTGACGTTGGACAAGC
TAATCCTGACGGAGGCGGCCCGGGGAAACTTCTGAGCCCCGCTCGCCGCCGGGCCTGTATTGACCATGTCCGCAGTGAGCTGGGCATTTCCGAGCGACGG
GCCTGCCAAGTGCTGCACCAGCACCGCTCAACACAGCGCAAGGTGCCACGAGGGCGGGCGGACGAAGATCTCCTGGTCGCCGACATGATTGAGCTGGCCC
AGCAATACGGCCGTTATGGCTATCGTCGGATTGCGGCTCTGTTGCGGGATGCCGGATGGCAGGTGAACGACAAGCGGGTCGAGCGCTTATGGCGGCGAGA
GGGACTGAAGGTGCCGGTAAAGCAACCAAAGAAGGGCCGACTATGGCTGAACGACGGATCCTGTGTCCGGTTGCGGCCGGAGTACCGGGATCATGTTTGG
TCATATGACTTCGTCCACTGCCGCACCGACGACGGCAAGGTGTTTCGAACGCTGAGCATCCTCGATGAATTCAGCCGCGAATGCCTGACGATCAGGGTGC
GGCGCAAGCTGAACTCAACAGATGTGATCGATGCGCTGACAGACCTGTTCATCATTCGCGGCGTGCCAGCCTTCATTCGTTCCGACAATGGCCCGGAGTT
CATAGCACAAGCCGTGCGAGACTGGATCAGCGCCGTCGGGGCAAAGACAGCTTACATCGAACCCGGATCGCCTTGGGAGAATGGGTATTGCGAGAGCTTC
AACGCCCGCTTTCGCGATGAAATGCTGAACGGGGAGGTCTTCTACAGCCTCCGCGAGGCACAGATCCTGATCGAGCAATGGAGGAAGCATTACAACACGA
AACGACCACACAGCGCATTGGGCTACCGCCCACCGGCCCCGGAAACCATCGTCCCGATGGACCAAAGGCCCGTCATGCACTAACTTTTAACTTGGACCAC
TCAGGTGGGGCTGATCA
GCGGCAGGTTGAGGTTCTGATGGGGCAAGGCTTGTCCCGTCTGGACGCGATCAGGCGTATCGGCGTTGTCGAACAGACCTATTACCGCTGGCGCAAGCAA
TACGGCGGAATGGGCGTAGACCAGTTGAAAGAGCTGAAGCGCCTTCAGCAGGAGAATGAGCGGCTCAGGCGTGCGGTGTCTGACCTGACGTTGGACAAGC
TAATCCTGACGGAGGCGGCCCGGGGAAACTTCTGAGCCCCGCTCGCCGCCGGGCCTGTATTGACCATGTCCGCAGTGAGCTGGGCATTTCCGAGCGACGG
GCCTGCCAAGTGCTGCACCAGCACCGCTCAACACAGCGCAAGGTGCCACGAGGGCGGGCGGACGAAGATCTCCTGGTCGCCGACATGATTGAGCTGGCCC
AGCAATACGGCCGTTATGGCTATCGTCGGATTGCGGCTCTGTTGCGGGATGCCGGATGGCAGGTGAACGACAAGCGGGTCGAGCGCTTATGGCGGCGAGA
GGGACTGAAGGTGCCGGTAAAGCAACCAAAGAAGGGCCGACTATGGCTGAACGACGGATCCTGTGTCCGGTTGCGGCCGGAGTACCGGGATCATGTTTGG
TCATATGACTTCGTCCACTGCCGCACCGACGACGGCAAGGTGTTTCGAACGCTGAGCATCCTCGATGAATTCAGCCGCGAATGCCTGACGATCAGGGTGC
GGCGCAAGCTGAACTCAACAGATGTGATCGATGCGCTGACAGACCTGTTCATCATTCGCGGCGTGCCAGCCTTCATTCGTTCCGACAATGGCCCGGAGTT
CATAGCACAAGCCGTGCGAGACTGGATCAGCGCCGTCGGGGCAAAGACAGCTTACATCGAACCCGGATCGCCTTGGGAGAATGGGTATTGCGAGAGCTTC
AACGCCCGCTTTCGCGATGAAATGCTGAACGGGGAGGTCTTCTACAGCCTCCGCGAGGCACAGATCCTGATCGAGCAATGGAGGAAGCATTACAACACGA
AACGACCACACAGCGCATTGGGCTACCGCCCACCGGCCCCGGAAACCATCGTCCCGATGGACCAAAGGCCCGTCATGCACTAACTTTTAACTTGGACCAC
TCAGGTGGGGCTGATCA
Recoding section
- Recoding by frameshift
- Frame -1
- Type translational
- Experimentally demonstrated No
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 279 bp | 92 aa | 57 | 335 | + | No |
Description : First part of the transposase
ORF sequence :
MANKRPKPEEIVSKLRQVEVLMGQGLSRLDAIRRIGVVEQTYYRWRKQYGGMGVDQLKELKRLQQENERLRRAVSDLTLDKLILTEAARGNF
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 900 bp | 299 aa | 284 | 1183 | + | No |
Description : Second part of the transposase
ORF sequence :
PDVGQANPDGGGPGKLLSPARRRACIDHVRSELGISERRACQVLHQHRSTQRKVPRGRADEDLLVADMIELAQQYGRYGYRRIAALLRDAGWQVNDKRVE
RLWRREGLKVPVKQPKKGRLWLNDGSCVRLRPEYRDHVWSYDFVHCRTDDGKVFRTLSILDEFSRECLTIRVRRKLNSTDVIDALTDLFIIRGVPAFIRS
DNGPEFIAQAVRDWISAVGAKTAYIEPGSPWENGYCESFNARFRDEMLNGEVFYSLREAQILIEQWRKHYNTKRPHSALGYRPPAPETIVPMDQRPVMH
RLWRREGLKVPVKQPKKGRLWLNDGSCVRLRPEYRDHVWSYDFVHCRTDDGKVFRTLSILDEFSRECLTIRVRRKLNSTDVIDALTDLFIIRGVPAFIRS
DNGPEFIAQAVRDWISAVGAKTAYIEPGSPWENGYCESFNARFRDEMLNGEVFYSLREAQILIEQWRKHYNTKRPHSALGYRPPAPETIVPMDQRPVMH
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1127 bp | 375 aa | 57 | 1183 | + | Yes |
Chemistry : DDE
ORF sequence :
MANKRPKPEEIVSKLRQVEVLMGQGLSRLDAIRRIGVVEQTYYRWRKQYGGMGVDQLKELKRLQQENERLRRAVSDLTLDKLILTEAARGKLLSPARRRA
CIDHVRSELGISERRACQVLHQHRSTQRKVPRGRADEDLLVADMIELAQQYGRYGYRRIAALLRDAGWQVNDKRVERLWRREGLKVPVKQPKKGRLWLND
GSCVRLRPEYRDHVWSYDFVHCRTDDGKVFRTLSILDEFSRECLTIRVRRKLNSTDVIDALTDLFIIRGVPAFIRSDNGPEFIAQAVRDWISAVGAKTAY
IEPGSPWENGYCESFNARFRDEMLNGEVFYSLREAQILIEQWRKHYNTKRPHSALGYRPPAPETIVPMDQRPVMH
CIDHVRSELGISERRACQVLHQHRSTQRKVPRGRADEDLLVADMIELAQQYGRYGYRRIAALLRDAGWQVNDKRVERLWRREGLKVPVKQPKKGRLWLND
GSCVRLRPEYRDHVWSYDFVHCRTDDGKVFRTLSILDEFSRECLTIRVRRKLNSTDVIDALTDLFIIRGVPAFIRSDNGPEFIAQAVRDWISAVGAKTAY
IEPGSPWENGYCESFNARFRDEMLNGEVFYSLREAQILIEQWRKHYNTKRPHSALGYRPPAPETIVPMDQRPVMH
Blast result :
Comments
ISPye6 is 93% aa similar to ISRosp5.
ISPye6 was identified by in silico nucleotide sequence analysis of Paracoccus yeei strain FDAARGOS_252. The third ORF is the putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
ISPye6 was identified by in silico nucleotide sequence analysis of Paracoccus yeei strain FDAARGOS_252. The third ORF is the putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References
1] Chmielowska C., Szuplewska M., Bartosik D. (2018) Direct submission.
2] Goldberg,B., Campos,J., Tallon,L., Sadzewicz,L., Sengamalay,N., Ott,S., Godinez,A., Nagaraj,S., Vavikolanu,K., Aluvathingal,J., Nadendla,S. and Sichtig,H. (2017) Direct GenBank submission.
2] Goldberg,B., Campos,J., Tallon,L., Sadzewicz,L., Sengamalay,N., Ott,S., Godinez,A., Nagaraj,S., Vavikolanu,K., Aluvathingal,J., Nadendla,S. and Sichtig,H. (2017) Direct GenBank submission.