ISPye3
- Family IS3
- Group IS150
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| CP020442 | ND | Paracoccus yeei | Paracoccus yeei Paracoccus yeei FDAARGOS_252 Paracoccus yeei CCUG 32053 |
DNA section
IS Length : 1270 bp
Ends
IR Length : 21/26
IRL : TGAGCTACCCCCCGAAAATCGGACAGTGACGGAAGCTACGATCTGAAGTC
IRR : TGAGCTACTCCCCGATCCTTGGACAGCTTTCCGGCTGATTTAAGCTACTG
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| ttggtgatca | ACT | tccaaccctg | 3 |
| tcacgcgacc | CGG | ttcttcagaa | 3 |
| cgctgtgtag | gaagtcatgg | 0 | |
| ctggcgcaac | ggcggatcgc | 0 |
DNA sequence
TGAGCTACCCCCCGAAAATCGGACAGTGACGGAAGCTACGATCTGAAGTCTGCTGGTCTCCAAGAACGAGGAGAACAGAGATGTCGAGACGCAGGAACCA
CGATGCGGCGTTCAAGGCGCGTGTGGCGCTGGAAGCCGTGAAGGGGGAGCGCACAGTGTCAGAGCTGGCCGCCGAATATGGCGTGCATCCGACGATGATC
CATCAATGGAAACGGTCGCTTCTGGAGGGTGCCGCAGGGATCTTCGAGCGGGGCGGCAAGGCTGCGGCAGCGGCCGAGGTTGCCGAAGAAACTGTCCGTG
ACCTGCACGCCAAGATCGGAGAGCTGGCCGTCGCCAACGATTTTTTGTCACGAAAGCTCAAGCCCTGGATCGGCAAGTGAAGCGCGGAATGGTTGAGAAG
AACCACCCAAGCCTGTCGGTCGGGGCGCAGTGCCGCCTGCTATCGATCTCGCGGTCGTCGTTCTATTACGCGCCGCAGGGCGAGACGGCGATGAACCTCG
ACCTCATGCTGTTGATCGACAAGCAGTTCCTGGAAGCTCCTTTCTACGGCGTGCGGCAGATGACGTGGCACCTGCAGAACGAGGGGCATGGTGTGAACCA
GAAACGCATCCGGCGGCTGATGCGGCTCATGCGCCTGATGCCGATCTACCAGAGGCCCAACACCAGCAAGCCGAGAAAGGGCCACAAGACCTATCCCTAT
ATGTTGGGCGGGCTGCGGGTGGATCGGCCCGGTCAGGTCTGGTGCGCAGATATCACCTATTTGCCGATGCGGCGGGGCTTTCTGTATCTGGTGGCGATCA
TGGACTGGCTCACCCGCAAGGTGCTGGCCTGGCGCATCTCCAACACGCTAGAGGCCGACTTCTGCGTCGAGGCGTTGAATGAGGCCATCCACCGCTTCGG
CGCGCCGGGGATCATGAATACGGACCAGGGCTCACAGTTCACGTCCTTCGCCTGGACTGATCGGCTGAAGCGCGTTGGCACCCGGATCTCCATGGACGGC
AAAGGACGCTGCATCGACAACGTCTTCATCGAGCGCCTCTGGCGATCCCTGAAGTATGAATGCGTCTATCTGCACGCCTGGGAAACGGGATCGCAGGCCA
AGGTTGGTATTGGGCACTGGATCAACTTCTACAACCATCGACGACCCCACACTGCCCATGGCGGGCAGCCGCCCGCCGTGGTCTACTTCAACAGCAGCGA
AACCGATCAGCAGGCACAGGCAGTAGCTTAAATCAGCCGGAAAGCTGTCCAAGGATCGGGGAGTAGCTCA
CGATGCGGCGTTCAAGGCGCGTGTGGCGCTGGAAGCCGTGAAGGGGGAGCGCACAGTGTCAGAGCTGGCCGCCGAATATGGCGTGCATCCGACGATGATC
CATCAATGGAAACGGTCGCTTCTGGAGGGTGCCGCAGGGATCTTCGAGCGGGGCGGCAAGGCTGCGGCAGCGGCCGAGGTTGCCGAAGAAACTGTCCGTG
ACCTGCACGCCAAGATCGGAGAGCTGGCCGTCGCCAACGATTTTTTGTCACGAAAGCTCAAGCCCTGGATCGGCAAGTGAAGCGCGGAATGGTTGAGAAG
AACCACCCAAGCCTGTCGGTCGGGGCGCAGTGCCGCCTGCTATCGATCTCGCGGTCGTCGTTCTATTACGCGCCGCAGGGCGAGACGGCGATGAACCTCG
ACCTCATGCTGTTGATCGACAAGCAGTTCCTGGAAGCTCCTTTCTACGGCGTGCGGCAGATGACGTGGCACCTGCAGAACGAGGGGCATGGTGTGAACCA
GAAACGCATCCGGCGGCTGATGCGGCTCATGCGCCTGATGCCGATCTACCAGAGGCCCAACACCAGCAAGCCGAGAAAGGGCCACAAGACCTATCCCTAT
ATGTTGGGCGGGCTGCGGGTGGATCGGCCCGGTCAGGTCTGGTGCGCAGATATCACCTATTTGCCGATGCGGCGGGGCTTTCTGTATCTGGTGGCGATCA
TGGACTGGCTCACCCGCAAGGTGCTGGCCTGGCGCATCTCCAACACGCTAGAGGCCGACTTCTGCGTCGAGGCGTTGAATGAGGCCATCCACCGCTTCGG
CGCGCCGGGGATCATGAATACGGACCAGGGCTCACAGTTCACGTCCTTCGCCTGGACTGATCGGCTGAAGCGCGTTGGCACCCGGATCTCCATGGACGGC
AAAGGACGCTGCATCGACAACGTCTTCATCGAGCGCCTCTGGCGATCCCTGAAGTATGAATGCGTCTATCTGCACGCCTGGGAAACGGGATCGCAGGCCA
AGGTTGGTATTGGGCACTGGATCAACTTCTACAACCATCGACGACCCCACACTGCCCATGGCGGGCAGCCGCCCGCCGTGGTCTACTTCAACAGCAGCGA
AACCGATCAGCAGGCACAGGCAGTAGCTTAAATCAGCCGGAAAGCTGTCCAAGGATCGGGGAGTAGCTCA
Recoding section
- Recoding by frameshift
- Frame -1
- Type translational
- Experimentally demonstrated No
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 300 bp | 99 aa | 81 | 380 | + | No |
Description : First part of the transposase
ORF sequence :
MSRRRNHDAAFKARVALEAVKGERTVSELAAEYGVHPTMIHQWKRSLLEGAAGIFERGGKAAAAAEVAEETVRDLHAKIGELAVANDFLSRKLKPWIGK
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 930 bp | 309 aa | 302 | 1231 | + | No |
Description : Second part of the transposase
ORF sequence :
PARQDRRAGRRQRFFVTKAQALDRQVKRGMVEKNHPSLSVGAQCRLLSISRSSFYYAPQGETAMNLDLMLLIDKQFLEAPFYGVRQMTWHLQNEGHGVNQ
KRIRRLMRLMRLMPIYQRPNTSKPRKGHKTYPYMLGGLRVDRPGQVWCADITYLPMRRGFLYLVAIMDWLTRKVLAWRISNTLEADFCVEALNEAIHRFG
APGIMNTDQGSQFTSFAWTDRLKRVGTRISMDGKGRCIDNVFIERLWRSLKYECVYLHAWETGSQAKVGIGHWINFYNHRRPHTAHGGQPPAVVYFNSSE
TDQQAQAVA
KRIRRLMRLMRLMPIYQRPNTSKPRKGHKTYPYMLGGLRVDRPGQVWCADITYLPMRRGFLYLVAIMDWLTRKVLAWRISNTLEADFCVEALNEAIHRFG
APGIMNTDQGSQFTSFAWTDRLKRVGTRISMDGKGRCIDNVFIERLWRSLKYECVYLHAWETGSQAKVGIGHWINFYNHRRPHTAHGGQPPAVVYFNSSE
TDQQAQAVA
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1151 bp | 383 aa | 81 | 1231 | + | Yes |
Chemistry : DDE
ORF sequence :
MSRRRNHDAAFKARVALEAVKGERTVSELAAEYGVHPTMIHQWKRSLLEGAAGIFERGGKAAAAAEVAEETVRDLHAKIGELAVANDFFVTKAQALDRQV
KRGMVEKNHPSLSVGAQCRLLSISRSSFYYAPQGETAMNLDLMLLIDKQFLEAPFYGVRQMTWHLQNEGHGVNQKRIRRLMRLMRLMPIYQRPNTSKPRK
GHKTYPYMLGGLRVDRPGQVWCADITYLPMRRGFLYLVAIMDWLTRKVLAWRISNTLEADFCVEALNEAIHRFGAPGIMNTDQGSQFTSFAWTDRLKRVG
TRISMDGKGRCIDNVFIERLWRSLKYECVYLHAWETGSQAKVGIGHWINFYNHRRPHTAHGGQPPAVVYFNSSETDQQAQAVA
KRGMVEKNHPSLSVGAQCRLLSISRSSFYYAPQGETAMNLDLMLLIDKQFLEAPFYGVRQMTWHLQNEGHGVNQKRIRRLMRLMRLMPIYQRPNTSKPRK
GHKTYPYMLGGLRVDRPGQVWCADITYLPMRRGFLYLVAIMDWLTRKVLAWRISNTLEADFCVEALNEAIHRFGAPGIMNTDQGSQFTSFAWTDRLKRVG
TRISMDGKGRCIDNVFIERLWRSLKYECVYLHAWETGSQAKVGIGHWINFYNHRRPHTAHGGQPPAVVYFNSSETDQQAQAVA
Blast result :
Comments
References
1] Chmielowska C., Szuplewska M., Bartosik D. (2018) Direct submission.
2] Goldberg,B., Campos,J., Tallon,L., Sadzewicz,L., Sengamalay,N., Ott,S., Godinez,A., Nagaraj,S., Vavikolanu,K., Aluvathingal,J., Nadendla,S. and Sichtig,H. (2017) Direct GenBank submission.
2] Goldberg,B., Campos,J., Tallon,L., Sadzewicz,L., Sengamalay,N., Ott,S., Godinez,A., Nagaraj,S., Vavikolanu,K., Aluvathingal,J., Nadendla,S. and Sichtig,H. (2017) Direct GenBank submission.