ISEcret5

  • Family IS630
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
KP890199 ND Endozoicomonas endosymbiont
Endozoicomonas endosymbiont of Diplodus puntazzo
DNA section
IS Length : 1149 bp

Ends


IR Length : 15/17

IRL : CCAATCGCTTTTTCTAAATCCTTTAGCTGATGCCAGCTATGCTCAGACAT
IRR : CCAATTGCCTTTTCTAACTACTGTATAACTGCCCACTGGCGAGAGCATAA

Insertion site


Left flankDirect repeatRight flankDR Length
GTTGAGGTTCTTATATTATGAGCTGGT2

DNA sequence

CCAATCGCTTTTTCTAAATCCTTTAGCTGATGCCAGCTATGCTCAGACATCATTATCAAGCGTTTTATCTGGAACAGATGTCTCGTCGACCACAGCTACC
CGAAGGTTTTGAGGATGTTGATTTTCAGCATGCTGCCCGTCATGCAGATGATAACCGTGAATGCATCCGGTTGTTGGGGCTGTCATTTATTCAACAAGGA
CACTCTGTTTCTGAGACATCCAAACTGCTTAAAGTCACCAATGATGCGGTACATGACTGGCTGCATCGATTTAAAGATGGCGGCCTTCCAGCTATGAAGG
ATCAAGGTGGAAGGGGACGAAAACCCATAATCCCTAAAGAATCTCATGATGAATTCAAAGAAGCCGTTCTCCACTTGCAGGACGAAAAATATGGAGGACG
TATAGTTGGTCGGGATGTTTATAAACTGTTACAAAATGAGTTTGGGACAGATTGCAACCTTCGCACCGTTTATAAACTGCTGCATAGAGTGAAGCTGTCG
TGGATTTCTGGTAGGTCCAAGCACCCAAAACAGGACCTTGAAGAACAGGAGGTTTTTAAAAAAACTTCAAGCAGGAGGTCGTGAAGGTTGTGCCTCTGGG
AGCCTGGGAAAAAGTCATTGATATCTGGTTCCAGGATGAAGCCAGGTTTGGTCAACAGAATTCAATCAGTCGGATATGGGCACCGACGGGCAGTCGCCCC
GGACTTATCCGGCAGCAACAGTTTGAGTATGCTTATGTATTCGGCGCCGTATGCCCTCAACAGGATAGAGCCATTGGCCTTGTTCTACCCTGTGCAAACG
CCAAAGGTCTTGCCCTTCACCTGGAGCTAATCTCTTTAGCGACTGAAGATGGGCATCATGCAGTGGTTCTTCTGGACAGGGCAGGATTCCATATGGCACA
GGATCTCCCGAAGTTTGAAAATGTCAGCCTGCTCTGGTTGCCAGCATGCGCTCCAGAATTAAACAGTTCAGAAAGATTGTGGGAGTGGATGCGGGAGCAT
GATTTGGCCAATAAAGCATTTAGCGGTTACGAAGATATCGTGGATTGCTGCTGCGAGGCCTGGAACAATCTATGCGGCGAAGCTGGACGTCTATTCAGTT
TATGCTCTCGCCAGTGGGCAGTTATACAGTAGTTAGAAAAGGCAATTGG
Recoding section
  • Recoding by frameshift
  • Frame -1
  • Type translational
  • Experimentally demonstrated No

Stimulators :

  • Shine-Dalgarno sequence :
  • Secondary structure :

Recoding motif :

Protein section
ORF number : 3

 

ORF 1
LengthBeginEndStrandFusion ORF
507 bp168 aa78584+No
ORF function : Transposase
Description : First part of the transposase

ORF sequence :

MSRRPQLPEGFEDVDFQHAARHADDNRECIRLLGLSFIQQGHSVSETSKLLKVTNDAVHDWLHRFKDGGLPAMKDQGGRGRKPIIPKESHDEFKEAVLHL
QDEKYGGRIVGRDVYKLLQNEFGTDCNLRTVYKLLHRVKLSWISGRSKHPKQDLEEQEVFKKTSSRRS

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
573 bp190 aa5601132+No
ORF function : Transposase
Description : Second part of the transposase

ORF sequence :

KNFKQEVVKVVPLGAWEKVIDIWFQDEARFGQQNSISRIWAPTGSRPGLIRQQQFEYAYVFGAVCPQQDRAIGLVLPCANAKGLALHLELISLATEDGHH
AVVLLDRAGFHMAQDLPKFENVSLLWLPACAPELNSSERLWEWMREHDLANKAFSGYEDIVDCCCEAWNNLCGEAGRLFSLCSRQWAVIQ

 

Blast result :
ORF 3
LengthBeginEndStrandFusion ORF
1055 bp351 aa781132+Yes
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MSRRPQLPEGFEDVDFQHAARHADDNRECIRLLGLSFIQQGHSVSETSKLLKVTNDAVHDWLHRFKDGGLPAMKDQGGRGRKPIIPKESHDEFKEAVLHL
QDEKYGGRIVGRDVYKLLQNEFGTDCNLRTVYKLLHRVKLSWISGRSKHPKQDLEEQEVFKKNFKQEVVKVVPLGAWEKVIDIWFQDEARFGQQNSISRI
WAPTGSRPGLIRQQQFEYAYVFGAVCPQQDRAIGLVLPCANAKGLALHLELISLATEDGHHAVVLLDRAGFHMAQDLPKFENVSLLWLPACAPELNSSER
LWEWMREHDLANKAFSGYEDIVDCCCEAWNNLCGEAGRLFSLCSRQWAVIQ

 

Blast result :
Comments
ISEcret5 is 62% aa similar to ISShfr2.
The third ORF is the putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References
1] Helena Seth-Smith (2015) Direct submission.