ISPa86
- Family IS3
- Group IS3
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| MF344569 | Y | Pseudomonas aeruginosa | Pseudomonas aeruginosa Pseudomonas aeruginosa 12939 Pseudomonas stutzeri DSM 4166 |
DNA section
IS Length : 1221 bp
Ends
IR Length : 24/29
IRL : GGGTTCTACCCCATTAGCACGGACACTTTCGAGTAAGCTCATGCCGAGCT
IRR : GGGTTCTACCCCGTTTCCACGGACGGTTTGAAAAGGGCTGAAAACTTCAG
Comments : Atypical ends for an IS3 family member.
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| TGGGTTGAGG | CCT | CGGCACGACT | 0 |
| TGACCGATAG | GTA | GCGGAGATCG | 0 |
| AGTAAAACA | GGGTAATCTG | 0 |
DNA sequence
GGGTTCTACCCCATTAGCACGGACACTTTCGAGTAAGCTCATGCCGAGCTGAAGGAGTGTTCATGAAGCGCAAGAAATACAGCCCCGAGTTCAAGCGGGA
AGCCATCGAGTTGGTTCGTCGTTCAGGGGCGAGCTGTCGACAGGTGGCCCTGGAGATTGGTGTGGCTCCGAACCTGCTCACACGCTGGGTTCGAGAGGCA
CAGCCAGGCGCAGAAAAGGCCTTTCCCGGAACAGGTAGTCCGCGAGATGAGGAGCTGGCCCGCCTCAAGCGTGAGTTGGCCCGGGTGACCAAGGAACGTG
ATTTTTTAAGAGACGCGGCAGCGTACTTTGCCAGGGAGTCATCGAGCGGTACACGGTGATCCAGCGCTGCCGCAACGAGTACCCGGTACGTCTGATGTGC
CGTTGCCTGAAGGTTTCTGCCAGTGGCTATTACGCCTGGCAGGATCGTGATCCAAGCCCGCGAACTCAAGAGAATGCACGTCTGGTCAGGCGCATTCGTG
AGATTCATGAGGACAGCCGTGGCGTGATCGGCGCACCGCGCATGCATGAGGATTTGCTCGACGAGGGCGAAATCGTCAGCTTGAACCGCGTTGCTCGCCT
GATGGCGGCTGAGCGAATTCAAGGCTGGCCACGCCGTAAGAGGCGTGGTTTTGGAAGAGTCGCCAGTGGCCGTCCAGCAGGCGTGAAAAACCTGCTGGAG
CGCGATTTCACCGCGCAGGAGCCGGAGCGCAAGTGGGTCACGGACATCACGGAAATAGCCACACTGGAAGGCAAGCTTTTCCTATGTGTGGTGCTCGACT
TGTACAGCAAGCTGGTGATCGGTTGGTCGATGCATCACCGCCAGGATCGGCAGATGGTGATTCGAGCAGTGGAGATGGCAATCTGGCAGCGCCAGGGTGA
CTGGTCAGTAATCCTGCATTCGGATCGCGGTAGCCAATTCACCAGCACCGACTACCAGCGCTTTCTGAATCGCAACACGCTGGTCTGCAGCATGAGTGCC
GTCGGGCATTGCGGCGACAACGCAGCCTGTGAAGGCTTCTTCGGTCAGCTCAAACGGGAGCGCGTTGTCCATCAGTCGTATCGAACTCGTGATGAAGCAC
GGGCGGATTTATTCGACTACATCGAGCGGTTTCATAACCCACGAATGCGTCGTAGAGTCGCCAGGCAAGATCTGAAGTTTTCAGCCCTTTTCAAACCGTC
CGTGGAAACGGGGTAGAACCC
AGCCATCGAGTTGGTTCGTCGTTCAGGGGCGAGCTGTCGACAGGTGGCCCTGGAGATTGGTGTGGCTCCGAACCTGCTCACACGCTGGGTTCGAGAGGCA
CAGCCAGGCGCAGAAAAGGCCTTTCCCGGAACAGGTAGTCCGCGAGATGAGGAGCTGGCCCGCCTCAAGCGTGAGTTGGCCCGGGTGACCAAGGAACGTG
ATTTTTTAAGAGACGCGGCAGCGTACTTTGCCAGGGAGTCATCGAGCGGTACACGGTGATCCAGCGCTGCCGCAACGAGTACCCGGTACGTCTGATGTGC
CGTTGCCTGAAGGTTTCTGCCAGTGGCTATTACGCCTGGCAGGATCGTGATCCAAGCCCGCGAACTCAAGAGAATGCACGTCTGGTCAGGCGCATTCGTG
AGATTCATGAGGACAGCCGTGGCGTGATCGGCGCACCGCGCATGCATGAGGATTTGCTCGACGAGGGCGAAATCGTCAGCTTGAACCGCGTTGCTCGCCT
GATGGCGGCTGAGCGAATTCAAGGCTGGCCACGCCGTAAGAGGCGTGGTTTTGGAAGAGTCGCCAGTGGCCGTCCAGCAGGCGTGAAAAACCTGCTGGAG
CGCGATTTCACCGCGCAGGAGCCGGAGCGCAAGTGGGTCACGGACATCACGGAAATAGCCACACTGGAAGGCAAGCTTTTCCTATGTGTGGTGCTCGACT
TGTACAGCAAGCTGGTGATCGGTTGGTCGATGCATCACCGCCAGGATCGGCAGATGGTGATTCGAGCAGTGGAGATGGCAATCTGGCAGCGCCAGGGTGA
CTGGTCAGTAATCCTGCATTCGGATCGCGGTAGCCAATTCACCAGCACCGACTACCAGCGCTTTCTGAATCGCAACACGCTGGTCTGCAGCATGAGTGCC
GTCGGGCATTGCGGCGACAACGCAGCCTGTGAAGGCTTCTTCGGTCAGCTCAAACGGGAGCGCGTTGTCCATCAGTCGTATCGAACTCGTGATGAAGCAC
GGGCGGATTTATTCGACTACATCGAGCGGTTTCATAACCCACGAATGCGTCGTAGAGTCGCCAGGCAAGATCTGAAGTTTTCAGCCCTTTTCAAACCGTC
CGTGGAAACGGGGTAGAACCC
Recoding section
- Recoding by frameshift
- Frame -1
- Type translational
- Experimentally demonstrated No
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 297 bp | 98 aa | 63 | 359 | + | No |
Description : First part of the transposase
ORF sequence :
MKRKKYSPEFKREAIELVRRSGASCRQVALEIGVAPNLLTRWVREAQPGAEKAFPGTGSPRDEELARLKRELARVTKERDFLRDAAAYFARESSSGTR
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 915 bp | 304 aa | 302 | 1216 | + | No |
Description : Second part of the transposase
ORF sequence :
FFKRRGSVLCQGVIERYTVIQRCRNEYPVRLMCRCLKVSASGYYAWQDRDPSPRTQENARLVRRIREIHEDSRGVIGAPRMHEDLLDEGEIVSLNRVARL
MAAERIQGWPRRKRRGFGRVASGRPAGVKNLLERDFTAQEPERKWVTDITEIATLEGKLFLCVVLDLYSKLVIGWSMHHRQDRQMVIRAVEMAIWQRQGD
WSVILHSDRGSQFTSTDYQRFLNRNTLVCSMSAVGHCGDNAACEGFFGQLKRERVVHQSYRTRDEARADLFDYIERFHNPRMRRRVARQDLKFSALFKPS
VETG
MAAERIQGWPRRKRRGFGRVASGRPAGVKNLLERDFTAQEPERKWVTDITEIATLEGKLFLCVVLDLYSKLVIGWSMHHRQDRQMVIRAVEMAIWQRQGD
WSVILHSDRGSQFTSTDYQRFLNRNTLVCSMSAVGHCGDNAACEGFFGQLKRERVVHQSYRTRDEARADLFDYIERFHNPRMRRRVARQDLKFSALFKPS
VETG
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1154 bp | 384 aa | 63 | 1216 | + | Yes |
Chemistry : DDE
ORF sequence :
MKRKKYSPEFKREAIELVRRSGASCRQVALEIGVAPNLLTRWVREAQPGAEKAFPGTGSPRDEELARLKRELARVTKERDFFKRRGSVLCQGVIERYTVI
QRCRNEYPVRLMCRCLKVSASGYYAWQDRDPSPRTQENARLVRRIREIHEDSRGVIGAPRMHEDLLDEGEIVSLNRVARLMAAERIQGWPRRKRRGFGRV
ASGRPAGVKNLLERDFTAQEPERKWVTDITEIATLEGKLFLCVVLDLYSKLVIGWSMHHRQDRQMVIRAVEMAIWQRQGDWSVILHSDRGSQFTSTDYQR
FLNRNTLVCSMSAVGHCGDNAACEGFFGQLKRERVVHQSYRTRDEARADLFDYIERFHNPRMRRRVARQDLKFSALFKPSVETG
QRCRNEYPVRLMCRCLKVSASGYYAWQDRDPSPRTQENARLVRRIREIHEDSRGVIGAPRMHEDLLDEGEIVSLNRVARLMAAERIQGWPRRKRRGFGRV
ASGRPAGVKNLLERDFTAQEPERKWVTDITEIATLEGKLFLCVVLDLYSKLVIGWSMHHRQDRQMVIRAVEMAIWQRQGDWSVILHSDRGSQFTSTDYQR
FLNRNTLVCSMSAVGHCGDNAACEGFFGQLKRERVVHQSYRTRDEARADLFDYIERFHNPRMRRRVARQDLKFSALFKPSVETG
Blast result :
Comments
ISPa86 is 81% aa similar to ISStma5.
The third ORF is the putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
Two copies of ISPa86 were identified in plasmid p12939-IMP from pseudomonas aeruginosa 12939.
The third ORF is the putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
Two copies of ISPa86 were identified in plasmid p12939-IMP from pseudomonas aeruginosa 12939.
References
1] Dongsheng Zhou (2018) Direct submission.