ISPa85
- Family IS21
- Group
Isoform Synonym(s)
Accession number | Transposition | Origin | Host |
---|---|---|---|
MF344570 | ND | Pseudomonas aeruginosa | Pseudomonas aeruginosa Pseudomonas aeruginosa A681 |
DNA section
IS Length : 2595 bp
Ends
IR Length : 40/48
IRL : TGCGGATTCCACGCTGACTCGGACACCCATTCCACGCACATCCGGACAGT
IRR : TGCGGATTCCACGCCATTCGGACACTCAGCCCACGCTGATCCGGACACCT
Insertion site
Left flank | Direct repeat | Right flank | DR Length |
---|---|---|---|
CCACCGCGTA | CCCACAGT | CCAATCGCAC | 8 |
GGATAAATGG | ACGAAGGT | AGCAGGGCCA | 8 |
DNA sequence
TGCGGATTCCACGCTGACTCGGACACCCATTCCACGCACATCCGGACAGTGATTCCACGCTGATCCGGACACTCATTCCACGAGCATCCGGACACCGATT
CCACGGCCATCCGGACACTTTCCAGGCAGGCAGCTACGCAGGATTTATTCACTACCATCGACCTCTTTTTCGAAGCAGAGAGGTCGTCGTGGAGCGTTTG
TCCATGCGTAAAATCCGTGAGGTGTTGCGCCTCAAGTTCGATTGCGGGCTGTCTGTCCGTAAGATCTCCCGCAGTCTGGGCATAGGCCACAGCAGTGCCG
GTGATTACCTCTGTCGCTTTGCCGCCAGCGGCCTCAGTTGGCCTTGCTCGTTGTCCGATGCGGAGTTGGAGCAGCAGCTGTTCCCGCCACCGCCGGCGGT
ACCCTCCGAGCAACGACCGCTGCCCGATTGGGCCATGATCCACGCCGAGCTACGCCGGCCGGGCGTGACCTTGGCGCTGCTCTGGCAGGAGTATCGCCTG
AGCCAGCCGCAGGGCTTTCAGTACAGCTGGTTCTGCGAGCACTACCGGGCCTGGCAGGGCAAGCTGGACGTGGTGATGCGCCAGGAGCATCGCGTCGGCG
AGAAGTTGTTCGTCGACTACGCCGGGCAGACGGTGCCGGTGATCGACCGCCACAGCGGCGAGATCCGCCAGGCGCAGGTGTTCGTCGCGGTGCTCGGCGC
ATCCAGCTACACCTTCGCCGAAGCCACTTGGTCGCAGCAGTTACCGGACTGGCTGGGCTCCCATGTCCGCTGCTTCGCCTTTCTCGGCGGCGTGCCGGAA
ATCGTGGTGCCGGACAACCTGCGAAGCGCGGTGAGCAAGAGCCATCGCTACGAGCCGGACATCAATCCCAGCTACCGCGACCTGGCCGAGCACTATGGCG
TGGCGGTGGTGCCGGCGCGGGCGCGTAAGCCGCGCGACAAGGCTAAGGCCGAGGTCGGCGTGCAGGTGGTCGAGCGCTGGATCCTCGCCGCGCTGAGGAA
TCGCCAGTTCTTCTCCCTGGACGAACTCAACAGCGCCATCGCCTTATTGCTGGAGCGCCTCAACCGACGACCGTTTCGCAAGCTGCCGGGCTCCCGGCAC
TCGGCCTTCGAAGCCCTGGATCGTCCAGCGCTGCGCCCACTGCCGGAGCAGCCCTACGTCTATGCCGAGTGGAAGAAGGCGCGGGTGCACATCGACTACC
ACGTCGAGGTCGATGGTCATTACTACTCGGTGCCGTACCAACTGGTGAAGAAACAACTGGAAGTACGCCTGACAGCGCGCACGGTGGAGTGCTTCCACGC
CAATCAGCGGGTGGCCAGCCACCTGCGCTCACCGCACAAGGGTCGGCACAGCACACAGACCGAGCACATGCCCAAGAGCCATCGCGAGCATGCCGAGTGG
ACGCCGCAACGGCTGATCCGCTGGGCCGAGCAGACCGGGCCGAACACGGCCGGCGTGATCAGCCACATCCTCGAACGGCGCATCCACCCAACCCAAGGCT
ACCGGGCCTGCCTAGGCATCCTGCGCCTCGGCAAGACCCATGGCGAAGTGCGCTTGGAGTTGGCCTGCCGTCGCGCCCTCAGCCTCGGTGCGTGCAGCTA
CAAGAGCCTCGAATCGATCCTGCGCCAGGGGCTGGAAAACCTGCCGCTAGCTCAAGCCAACCTGCCGCTGCTACCGGACGACCACGCCAACCTGCGCGGT
TCCGCCTACTACCACTGACCCAAGGAATCCCACCATGCTGCCCCATCCGACCCTGGACAAGCTCCAGACCTTGCGCCTGCACGGCATGCTCAAGGCCCTG
AGCGAACAACAAAAGACCCCGGACATCGACAGCTTGAGCTTCGAGGAACGCCTTGGTCTGCTGGTCGACCGCGAACTGACCGAGCGCGACGACAAGCGCC
TCAGCAGCCGCCTGCGTCAGGCCCGGCTCAAGCACAACGCCTGCCTCGAAGACATCGACTACCGCAGCCCGCGCGGACTCGACAAGGCGCTGATCCTCCA
GCTGAGCAGCGGTCAGTGGTTACGCGACGGACTCAACCTGATTATCAACGGCCCGACTGGCGTCGGTAAGACCTGGCTGGCCTGCGCCCTGGCCCACCAG
GCCTGCCGAGAGGGTTACAGCGTGCGTTACCTGCGCTTGCCGCGCCTGTTGGAAGAGTTGGGCCTAGCCCACGGCGACGGGCGCTTCGCCAAGCTGATGA
GCGGCTATGCCAAGACCGACCTGCTGATCCTTGATGACTGGGGTCTGGCCCCGTTCAACGCGGAACAGCGGCGCGACATGCTGGAGCTACTGGACGACCG
CTACGGCCAGCGCTCGACCCTCGTGACCAGCCAAATGCCCGTGGACAACTGGCACGAACTGATCGGCGATCCAACCCTGGCCGATGCCATCCTCGACCGC
CTGGTGCACAACGCTTATCGGATCAACCTCAAGGGTGAGTCGATGCGCAAACAGACGAAGAAATTGACGACACCGGGCACCTCAGACTAACAATGCAACC
CCTGCGTCGCTGCGCTCCGACTGCCTGTCCGAATGGCCGTGGAACAGGTGTCCGGATCAGCGTGGGCTGAGTGTCCGAATGGCGTGGAATCCGCA
CCACGGCCATCCGGACACTTTCCAGGCAGGCAGCTACGCAGGATTTATTCACTACCATCGACCTCTTTTTCGAAGCAGAGAGGTCGTCGTGGAGCGTTTG
TCCATGCGTAAAATCCGTGAGGTGTTGCGCCTCAAGTTCGATTGCGGGCTGTCTGTCCGTAAGATCTCCCGCAGTCTGGGCATAGGCCACAGCAGTGCCG
GTGATTACCTCTGTCGCTTTGCCGCCAGCGGCCTCAGTTGGCCTTGCTCGTTGTCCGATGCGGAGTTGGAGCAGCAGCTGTTCCCGCCACCGCCGGCGGT
ACCCTCCGAGCAACGACCGCTGCCCGATTGGGCCATGATCCACGCCGAGCTACGCCGGCCGGGCGTGACCTTGGCGCTGCTCTGGCAGGAGTATCGCCTG
AGCCAGCCGCAGGGCTTTCAGTACAGCTGGTTCTGCGAGCACTACCGGGCCTGGCAGGGCAAGCTGGACGTGGTGATGCGCCAGGAGCATCGCGTCGGCG
AGAAGTTGTTCGTCGACTACGCCGGGCAGACGGTGCCGGTGATCGACCGCCACAGCGGCGAGATCCGCCAGGCGCAGGTGTTCGTCGCGGTGCTCGGCGC
ATCCAGCTACACCTTCGCCGAAGCCACTTGGTCGCAGCAGTTACCGGACTGGCTGGGCTCCCATGTCCGCTGCTTCGCCTTTCTCGGCGGCGTGCCGGAA
ATCGTGGTGCCGGACAACCTGCGAAGCGCGGTGAGCAAGAGCCATCGCTACGAGCCGGACATCAATCCCAGCTACCGCGACCTGGCCGAGCACTATGGCG
TGGCGGTGGTGCCGGCGCGGGCGCGTAAGCCGCGCGACAAGGCTAAGGCCGAGGTCGGCGTGCAGGTGGTCGAGCGCTGGATCCTCGCCGCGCTGAGGAA
TCGCCAGTTCTTCTCCCTGGACGAACTCAACAGCGCCATCGCCTTATTGCTGGAGCGCCTCAACCGACGACCGTTTCGCAAGCTGCCGGGCTCCCGGCAC
TCGGCCTTCGAAGCCCTGGATCGTCCAGCGCTGCGCCCACTGCCGGAGCAGCCCTACGTCTATGCCGAGTGGAAGAAGGCGCGGGTGCACATCGACTACC
ACGTCGAGGTCGATGGTCATTACTACTCGGTGCCGTACCAACTGGTGAAGAAACAACTGGAAGTACGCCTGACAGCGCGCACGGTGGAGTGCTTCCACGC
CAATCAGCGGGTGGCCAGCCACCTGCGCTCACCGCACAAGGGTCGGCACAGCACACAGACCGAGCACATGCCCAAGAGCCATCGCGAGCATGCCGAGTGG
ACGCCGCAACGGCTGATCCGCTGGGCCGAGCAGACCGGGCCGAACACGGCCGGCGTGATCAGCCACATCCTCGAACGGCGCATCCACCCAACCCAAGGCT
ACCGGGCCTGCCTAGGCATCCTGCGCCTCGGCAAGACCCATGGCGAAGTGCGCTTGGAGTTGGCCTGCCGTCGCGCCCTCAGCCTCGGTGCGTGCAGCTA
CAAGAGCCTCGAATCGATCCTGCGCCAGGGGCTGGAAAACCTGCCGCTAGCTCAAGCCAACCTGCCGCTGCTACCGGACGACCACGCCAACCTGCGCGGT
TCCGCCTACTACCACTGACCCAAGGAATCCCACCATGCTGCCCCATCCGACCCTGGACAAGCTCCAGACCTTGCGCCTGCACGGCATGCTCAAGGCCCTG
AGCGAACAACAAAAGACCCCGGACATCGACAGCTTGAGCTTCGAGGAACGCCTTGGTCTGCTGGTCGACCGCGAACTGACCGAGCGCGACGACAAGCGCC
TCAGCAGCCGCCTGCGTCAGGCCCGGCTCAAGCACAACGCCTGCCTCGAAGACATCGACTACCGCAGCCCGCGCGGACTCGACAAGGCGCTGATCCTCCA
GCTGAGCAGCGGTCAGTGGTTACGCGACGGACTCAACCTGATTATCAACGGCCCGACTGGCGTCGGTAAGACCTGGCTGGCCTGCGCCCTGGCCCACCAG
GCCTGCCGAGAGGGTTACAGCGTGCGTTACCTGCGCTTGCCGCGCCTGTTGGAAGAGTTGGGCCTAGCCCACGGCGACGGGCGCTTCGCCAAGCTGATGA
GCGGCTATGCCAAGACCGACCTGCTGATCCTTGATGACTGGGGTCTGGCCCCGTTCAACGCGGAACAGCGGCGCGACATGCTGGAGCTACTGGACGACCG
CTACGGCCAGCGCTCGACCCTCGTGACCAGCCAAATGCCCGTGGACAACTGGCACGAACTGATCGGCGATCCAACCCTGGCCGATGCCATCCTCGACCGC
CTGGTGCACAACGCTTATCGGATCAACCTCAAGGGTGAGTCGATGCGCAAACAGACGAAGAAATTGACGACACCGGGCACCTCAGACTAACAATGCAACC
CCTGCGTCGCTGCGCTCCGACTGCCTGTCCGAATGGCCGTGGAACAGGTGTCCGGATCAGCGTGGGCTGAGTGTCCGAATGGCGTGGAATCCGCA
Protein section
ORF number : 2
ORF 1
Length | Begin | End | Strand | Fusion ORF | |
---|---|---|---|---|---|
1515 bp | 504 aa | 204 | 1718 | + | No |
Chemistry : DDE
ORF sequence :
MRKIREVLRLKFDCGLSVRKISRSLGIGHSSAGDYLCRFAASGLSWPCSLSDAELEQQLFPPPPAVPSEQRPLPDWAMIHAELRRPGVTLALLWQEYRLS
QPQGFQYSWFCEHYRAWQGKLDVVMRQEHRVGEKLFVDYAGQTVPVIDRHSGEIRQAQVFVAVLGASSYTFAEATWSQQLPDWLGSHVRCFAFLGGVPEI
VVPDNLRSAVSKSHRYEPDINPSYRDLAEHYGVAVVPARARKPRDKAKAEVGVQVVERWILAALRNRQFFSLDELNSAIALLLERLNRRPFRKLPGSRHS
AFEALDRPALRPLPEQPYVYAEWKKARVHIDYHVEVDGHYYSVPYQLVKKQLEVRLTARTVECFHANQRVASHLRSPHKGRHSTQTEHMPKSHREHAEWT
PQRLIRWAEQTGPNTAGVISHILERRIHPTQGYRACLGILRLGKTHGEVRLELACRRALSLGACSYKSLESILRQGLENLPLAQANLPLLPDDHANLRGS
AYYH
QPQGFQYSWFCEHYRAWQGKLDVVMRQEHRVGEKLFVDYAGQTVPVIDRHSGEIRQAQVFVAVLGASSYTFAEATWSQQLPDWLGSHVRCFAFLGGVPEI
VVPDNLRSAVSKSHRYEPDINPSYRDLAEHYGVAVVPARARKPRDKAKAEVGVQVVERWILAALRNRQFFSLDELNSAIALLLERLNRRPFRKLPGSRHS
AFEALDRPALRPLPEQPYVYAEWKKARVHIDYHVEVDGHYYSVPYQLVKKQLEVRLTARTVECFHANQRVASHLRSPHKGRHSTQTEHMPKSHREHAEWT
PQRLIRWAEQTGPNTAGVISHILERRIHPTQGYRACLGILRLGKTHGEVRLELACRRALSLGACSYKSLESILRQGLENLPLAQANLPLLPDDHANLRGS
AYYH
Blast result :ORF 2
Length | Begin | End | Strand | Fusion ORF | |
---|---|---|---|---|---|
756 bp | 251 aa | 1735 | 2490 | + | No |
AG : IS21 helper
ORF sequence :
MLPHPTLDKLQTLRLHGMLKALSEQQKTPDIDSLSFEERLGLLVDRELTERDDKRLSSRLRQARLKHNACLEDIDYRSPRGLDKALILQLSSGQWLRDGL
NLIINGPTGVGKTWLACALAHQACREGYSVRYLRLPRLLEELGLAHGDGRFAKLMSGYAKTDLLILDDWGLAPFNAEQRRDMLELLDDRYGQRSTLVTSQ
MPVDNWHELIGDPTLADAILDRLVHNAYRINLKGESMRKQTKKLTTPGTSD
NLIINGPTGVGKTWLACALAHQACREGYSVRYLRLPRLLEELGLAHGDGRFAKLMSGYAKTDLLILDDWGLAPFNAEQRRDMLELLDDRYGQRSTLVTSQ
MPVDNWHELIGDPTLADAILDRLVHNAYRINLKGESMRKQTKKLTTPGTSD
Blast result :
Comments
ISPa85 is 97% (transposase) aa similar to ISPpu23, and has two copies in plasmid pA681-IMP from Pseudomonas aeruginosa strain A681.
References
1] Dongsheng Zhou (2018) Direct submission.