ISPa85

  • Family IS21
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
MF344570 ND Pseudomonas aeruginosa
Pseudomonas aeruginosa
Pseudomonas aeruginosa A681
DNA section
IS Length : 2595 bp

Ends


IR Length : 40/48

IRL : TGCGGATTCCACGCTGACTCGGACACCCATTCCACGCACATCCGGACAGT
IRR : TGCGGATTCCACGCCATTCGGACACTCAGCCCACGCTGATCCGGACACCT

Insertion site


Left flankDirect repeatRight flankDR Length
CCACCGCGTACCCACAGTCCAATCGCAC8
GGATAAATGGACGAAGGTAGCAGGGCCA8

DNA sequence

TGCGGATTCCACGCTGACTCGGACACCCATTCCACGCACATCCGGACAGTGATTCCACGCTGATCCGGACACTCATTCCACGAGCATCCGGACACCGATT
CCACGGCCATCCGGACACTTTCCAGGCAGGCAGCTACGCAGGATTTATTCACTACCATCGACCTCTTTTTCGAAGCAGAGAGGTCGTCGTGGAGCGTTTG
TCCATGCGTAAAATCCGTGAGGTGTTGCGCCTCAAGTTCGATTGCGGGCTGTCTGTCCGTAAGATCTCCCGCAGTCTGGGCATAGGCCACAGCAGTGCCG
GTGATTACCTCTGTCGCTTTGCCGCCAGCGGCCTCAGTTGGCCTTGCTCGTTGTCCGATGCGGAGTTGGAGCAGCAGCTGTTCCCGCCACCGCCGGCGGT
ACCCTCCGAGCAACGACCGCTGCCCGATTGGGCCATGATCCACGCCGAGCTACGCCGGCCGGGCGTGACCTTGGCGCTGCTCTGGCAGGAGTATCGCCTG
AGCCAGCCGCAGGGCTTTCAGTACAGCTGGTTCTGCGAGCACTACCGGGCCTGGCAGGGCAAGCTGGACGTGGTGATGCGCCAGGAGCATCGCGTCGGCG
AGAAGTTGTTCGTCGACTACGCCGGGCAGACGGTGCCGGTGATCGACCGCCACAGCGGCGAGATCCGCCAGGCGCAGGTGTTCGTCGCGGTGCTCGGCGC
ATCCAGCTACACCTTCGCCGAAGCCACTTGGTCGCAGCAGTTACCGGACTGGCTGGGCTCCCATGTCCGCTGCTTCGCCTTTCTCGGCGGCGTGCCGGAA
ATCGTGGTGCCGGACAACCTGCGAAGCGCGGTGAGCAAGAGCCATCGCTACGAGCCGGACATCAATCCCAGCTACCGCGACCTGGCCGAGCACTATGGCG
TGGCGGTGGTGCCGGCGCGGGCGCGTAAGCCGCGCGACAAGGCTAAGGCCGAGGTCGGCGTGCAGGTGGTCGAGCGCTGGATCCTCGCCGCGCTGAGGAA
TCGCCAGTTCTTCTCCCTGGACGAACTCAACAGCGCCATCGCCTTATTGCTGGAGCGCCTCAACCGACGACCGTTTCGCAAGCTGCCGGGCTCCCGGCAC
TCGGCCTTCGAAGCCCTGGATCGTCCAGCGCTGCGCCCACTGCCGGAGCAGCCCTACGTCTATGCCGAGTGGAAGAAGGCGCGGGTGCACATCGACTACC
ACGTCGAGGTCGATGGTCATTACTACTCGGTGCCGTACCAACTGGTGAAGAAACAACTGGAAGTACGCCTGACAGCGCGCACGGTGGAGTGCTTCCACGC
CAATCAGCGGGTGGCCAGCCACCTGCGCTCACCGCACAAGGGTCGGCACAGCACACAGACCGAGCACATGCCCAAGAGCCATCGCGAGCATGCCGAGTGG
ACGCCGCAACGGCTGATCCGCTGGGCCGAGCAGACCGGGCCGAACACGGCCGGCGTGATCAGCCACATCCTCGAACGGCGCATCCACCCAACCCAAGGCT
ACCGGGCCTGCCTAGGCATCCTGCGCCTCGGCAAGACCCATGGCGAAGTGCGCTTGGAGTTGGCCTGCCGTCGCGCCCTCAGCCTCGGTGCGTGCAGCTA
CAAGAGCCTCGAATCGATCCTGCGCCAGGGGCTGGAAAACCTGCCGCTAGCTCAAGCCAACCTGCCGCTGCTACCGGACGACCACGCCAACCTGCGCGGT
TCCGCCTACTACCACTGACCCAAGGAATCCCACCATGCTGCCCCATCCGACCCTGGACAAGCTCCAGACCTTGCGCCTGCACGGCATGCTCAAGGCCCTG
AGCGAACAACAAAAGACCCCGGACATCGACAGCTTGAGCTTCGAGGAACGCCTTGGTCTGCTGGTCGACCGCGAACTGACCGAGCGCGACGACAAGCGCC
TCAGCAGCCGCCTGCGTCAGGCCCGGCTCAAGCACAACGCCTGCCTCGAAGACATCGACTACCGCAGCCCGCGCGGACTCGACAAGGCGCTGATCCTCCA
GCTGAGCAGCGGTCAGTGGTTACGCGACGGACTCAACCTGATTATCAACGGCCCGACTGGCGTCGGTAAGACCTGGCTGGCCTGCGCCCTGGCCCACCAG
GCCTGCCGAGAGGGTTACAGCGTGCGTTACCTGCGCTTGCCGCGCCTGTTGGAAGAGTTGGGCCTAGCCCACGGCGACGGGCGCTTCGCCAAGCTGATGA
GCGGCTATGCCAAGACCGACCTGCTGATCCTTGATGACTGGGGTCTGGCCCCGTTCAACGCGGAACAGCGGCGCGACATGCTGGAGCTACTGGACGACCG
CTACGGCCAGCGCTCGACCCTCGTGACCAGCCAAATGCCCGTGGACAACTGGCACGAACTGATCGGCGATCCAACCCTGGCCGATGCCATCCTCGACCGC
CTGGTGCACAACGCTTATCGGATCAACCTCAAGGGTGAGTCGATGCGCAAACAGACGAAGAAATTGACGACACCGGGCACCTCAGACTAACAATGCAACC
CCTGCGTCGCTGCGCTCCGACTGCCTGTCCGAATGGCCGTGGAACAGGTGTCCGGATCAGCGTGGGCTGAGTGTCCGAATGGCGTGGAATCCGCA
Protein section
ORF number : 2

 

ORF 1
LengthBeginEndStrandFusion ORF
1515 bp504 aa2041718+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MRKIREVLRLKFDCGLSVRKISRSLGIGHSSAGDYLCRFAASGLSWPCSLSDAELEQQLFPPPPAVPSEQRPLPDWAMIHAELRRPGVTLALLWQEYRLS
QPQGFQYSWFCEHYRAWQGKLDVVMRQEHRVGEKLFVDYAGQTVPVIDRHSGEIRQAQVFVAVLGASSYTFAEATWSQQLPDWLGSHVRCFAFLGGVPEI
VVPDNLRSAVSKSHRYEPDINPSYRDLAEHYGVAVVPARARKPRDKAKAEVGVQVVERWILAALRNRQFFSLDELNSAIALLLERLNRRPFRKLPGSRHS
AFEALDRPALRPLPEQPYVYAEWKKARVHIDYHVEVDGHYYSVPYQLVKKQLEVRLTARTVECFHANQRVASHLRSPHKGRHSTQTEHMPKSHREHAEWT
PQRLIRWAEQTGPNTAGVISHILERRIHPTQGYRACLGILRLGKTHGEVRLELACRRALSLGACSYKSLESILRQGLENLPLAQANLPLLPDDHANLRGS
AYYH

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
756 bp251 aa17352490+No
ORF function : Accessory Gene
AG : IS21 helper

ORF sequence :

MLPHPTLDKLQTLRLHGMLKALSEQQKTPDIDSLSFEERLGLLVDRELTERDDKRLSSRLRQARLKHNACLEDIDYRSPRGLDKALILQLSSGQWLRDGL
NLIINGPTGVGKTWLACALAHQACREGYSVRYLRLPRLLEELGLAHGDGRFAKLMSGYAKTDLLILDDWGLAPFNAEQRRDMLELLDDRYGQRSTLVTSQ
MPVDNWHELIGDPTLADAILDRLVHNAYRINLKGESMRKQTKKLTTPGTSD

 

Blast result :
Comments
ISPa85 is 97% (transposase) aa similar to ISPpu23, and has two copies in plasmid pA681-IMP from Pseudomonas aeruginosa strain A681.
References
1] Dongsheng Zhou (2018) Direct submission.