ISStrsp1
- Family IS3
- Group IS150
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| CP006776 | ND | Streptococcus sp. | Streptococcus sp. I-P16 |
DNA section
IS Length : 1467 bp
Ends
IR Length : 21/26
IRL : TGATATGTACCCTCTTTACTGGACACCCAGTAAGGAGGGTATTTTCATGC
IRR : TGATATGTACCCAGAATCCTGGACACATGTTATGAACTAAGTGGAACACA
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| ACCTGTCTGT | AAAA | CACAGACAGG | 4 |
DNA sequence
TGATATGTACCCTCTTTACTGGACACCCAGTAAGGAGGGTATTTTCATGCGTTACAGTCATGAGTATAAGTTAGAGTGTATTGAGCTATATAGAAAAGGA
ATATGGCCTGAAACACCAGAAGGAATAAAAACATCAAACTTTCATCTAATGGTTCGATACTGGGCTCGTATCGAAGAAGAAAATGGACCAGATGCATTAA
AGCACTTAGGTAATAACAAAGTGTGGACCCCTGAAGCGAAATATGAATTAGTTGCTAAAGTATTAGCAGGACAATCCAACAAGTCAGTTGCTGTTTCTGC
AGGTATAAATAATGGAATGCTATATACCTGGGTTCGCAAATACAAAGAATTAGGTTACAATGGTCTTGTGAATAAAAAGAAAGGTCGCAAGAGTAAGAAC
CCTGTTATGAAAAAGAAAACGATTAAGCCCAAACCTTTAACTGAATCAGAACGTGAAGAGTTAATCAGACTAAGAGAAGAAATTGCGGCAATGAAAGCGG
AGATTGAAGTAGTAAAAAAAAGGATCGCCTTGAGGCAAGAAAGATGGGCTGCGCAACTCAAGGCGAAAAAGCAGCAATCATCAAAGCGCTCAGAGAAGAA
GGATACCAATTAAAGCACCTACTAAAAGGTTTAAATATGCCTAAATCAACTTACTACTACGAAATCAGTAAAGTTGATACTGTAGACTTTAGAAATATTA
AGCTCACTGAAGAAATCAAGAAAATATTTAGCCAGCATAAAGGTAGATATGGTGTGAGAAGAGTGTATAGAGAGCTCTTAAGACGTGGAAATTCTGTCAA
TCATAAAAGAGTACAACGTATCATGCATTCACTTGGATTGCAAGGCAAAAGACCTAAAGAAAAGTATCACTCATTCAAGGGTGAAGTAGGTAAAGTAGCA
CCAAACATAATAGATAGAGACTTTACAGCAACAGCACCACTTCAGAAGTGGACAACTGATGTATCTCAGTTTAACTTTTCATGGGGAAAGTGTTACCTTT
CTCCGATAATTGATATGTACACAAATGAAGTTATTTCATATGACTTGTCGATGAGTCCAAACCTAAATCAAATCAAGAGAATGCTAGAGATGGCGTTTAA
AAAGTTCAAATCACTTACAGGATTAATATTTCATTCAGATCAAGGATGGCAATATCAACACAACTACTATCGTCAGGAACTAAAAAACAGGGGAATTATC
CAATCAATGTCTAGACGTGGCAATTGTATAGATAATTGCATTATGGAAACATTCTTTGGACGTCTAAAGAATGAAATGTACTACAGCTACGAGAAAGAAT
ATGAATCATTTGAGAGCTTTTCAAAAGCACTAGATGAATACATAAGTTATTACAACAATGAAAGAATACAAGGAAAAACAAAATGGATGCCACCTGTAAT
GTACAGGATAGCATCCATGTGTTCCACTTAGTTCATAACATGTGTCCAGGATTCTGGGTACATATCA
ATATGGCCTGAAACACCAGAAGGAATAAAAACATCAAACTTTCATCTAATGGTTCGATACTGGGCTCGTATCGAAGAAGAAAATGGACCAGATGCATTAA
AGCACTTAGGTAATAACAAAGTGTGGACCCCTGAAGCGAAATATGAATTAGTTGCTAAAGTATTAGCAGGACAATCCAACAAGTCAGTTGCTGTTTCTGC
AGGTATAAATAATGGAATGCTATATACCTGGGTTCGCAAATACAAAGAATTAGGTTACAATGGTCTTGTGAATAAAAAGAAAGGTCGCAAGAGTAAGAAC
CCTGTTATGAAAAAGAAAACGATTAAGCCCAAACCTTTAACTGAATCAGAACGTGAAGAGTTAATCAGACTAAGAGAAGAAATTGCGGCAATGAAAGCGG
AGATTGAAGTAGTAAAAAAAAGGATCGCCTTGAGGCAAGAAAGATGGGCTGCGCAACTCAAGGCGAAAAAGCAGCAATCATCAAAGCGCTCAGAGAAGAA
GGATACCAATTAAAGCACCTACTAAAAGGTTTAAATATGCCTAAATCAACTTACTACTACGAAATCAGTAAAGTTGATACTGTAGACTTTAGAAATATTA
AGCTCACTGAAGAAATCAAGAAAATATTTAGCCAGCATAAAGGTAGATATGGTGTGAGAAGAGTGTATAGAGAGCTCTTAAGACGTGGAAATTCTGTCAA
TCATAAAAGAGTACAACGTATCATGCATTCACTTGGATTGCAAGGCAAAAGACCTAAAGAAAAGTATCACTCATTCAAGGGTGAAGTAGGTAAAGTAGCA
CCAAACATAATAGATAGAGACTTTACAGCAACAGCACCACTTCAGAAGTGGACAACTGATGTATCTCAGTTTAACTTTTCATGGGGAAAGTGTTACCTTT
CTCCGATAATTGATATGTACACAAATGAAGTTATTTCATATGACTTGTCGATGAGTCCAAACCTAAATCAAATCAAGAGAATGCTAGAGATGGCGTTTAA
AAAGTTCAAATCACTTACAGGATTAATATTTCATTCAGATCAAGGATGGCAATATCAACACAACTACTATCGTCAGGAACTAAAAAACAGGGGAATTATC
CAATCAATGTCTAGACGTGGCAATTGTATAGATAATTGCATTATGGAAACATTCTTTGGACGTCTAAAGAATGAAATGTACTACAGCTACGAGAAAGAAT
ATGAATCATTTGAGAGCTTTTCAAAAGCACTAGATGAATACATAAGTTATTACAACAATGAAAGAATACAAGGAAAAACAAAATGGATGCCACCTGTAAT
GTACAGGATAGCATCCATGTGTTCCACTTAGTTCATAACATGTGTCCAGGATTCTGGGTACATATCA
Recoding section
- Recoding by frameshift
- Frame -1
- Type translational
- Experimentally demonstrated No
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 567 bp | 188 aa | 47 | 613 | + | No |
Description : First part of the transposase
ORF sequence :
MRYSHEYKLECIELYRKGIWPETPEGIKTSNFHLMVRYWARIEEENGPDALKHLGNNKVWTPEAKYELVAKVLAGQSNKSVAVSAGINNGMLYTWVRKYK
ELGYNGLVNKKKGRKSKNPVMKKKTIKPKPLTESEREELIRLREEIAAMKAEIEVVKKRIALRQERWAAQLKAKKQQSSKRSEKKDTN
ELGYNGLVNKKKGRKSKNPVMKKKTIKPKPLTESEREELIRLREEIAAMKAEIEVVKKRIALRQERWAAQLKAKKQQSSKRSEKKDTN
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 924 bp | 307 aa | 508 | 1431 | + | No |
Description : Second part of the transposase
ORF sequence :
SSKKKDRLEARKMGCATQGEKAAIIKALREEGYQLKHLLKGLNMPKSTYYYEISKVDTVDFRNIKLTEEIKKIFSQHKGRYGVRRVYRELLRRGNSVNHK
RVQRIMHSLGLQGKRPKEKYHSFKGEVGKVAPNIIDRDFTATAPLQKWTTDVSQFNFSWGKCYLSPIIDMYTNEVISYDLSMSPNLNQIKRMLEMAFKKF
KSLTGLIFHSDQGWQYQHNYYRQELKNRGIIQSMSRRGNCIDNCIMETFFGRLKNEMYYSYEKEYESFESFSKALDEYISYYNNERIQGKTKWMPPVMYR
IASMCST
RVQRIMHSLGLQGKRPKEKYHSFKGEVGKVAPNIIDRDFTATAPLQKWTTDVSQFNFSWGKCYLSPIIDMYTNEVISYDLSMSPNLNQIKRMLEMAFKKF
KSLTGLIFHSDQGWQYQHNYYRQELKNRGIIQSMSRRGNCIDNCIMETFFGRLKNEMYYSYEKEYESFESFSKALDEYISYYNNERIQGKTKWMPPVMYR
IASMCST
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1385 bp | 461 aa | 47 | 1431 | + | Yes |
Chemistry : DDE
ORF sequence :
MRYSHEYKLECIELYRKGIWPETPEGIKTSNFHLMVRYWARIEEENGPDALKHLGNNKVWTPEAKYELVAKVLAGQSNKSVAVSAGINNGMLYTWVRKYK
ELGYNGLVNKKKGRKSKNPVMKKKTIKPKPLTESEREELIRLREEIAAMKAEIEVVKKKDRLEARKMGCATQGEKAAIIKALREEGYQLKHLLKGLNMPK
STYYYEISKVDTVDFRNIKLTEEIKKIFSQHKGRYGVRRVYRELLRRGNSVNHKRVQRIMHSLGLQGKRPKEKYHSFKGEVGKVAPNIIDRDFTATAPLQ
KWTTDVSQFNFSWGKCYLSPIIDMYTNEVISYDLSMSPNLNQIKRMLEMAFKKFKSLTGLIFHSDQGWQYQHNYYRQELKNRGIIQSMSRRGNCIDNCIM
ETFFGRLKNEMYYSYEKEYESFESFSKALDEYISYYNNERIQGKTKWMPPVMYRIASMCST
ELGYNGLVNKKKGRKSKNPVMKKKTIKPKPLTESEREELIRLREEIAAMKAEIEVVKKKDRLEARKMGCATQGEKAAIIKALREEGYQLKHLLKGLNMPK
STYYYEISKVDTVDFRNIKLTEEIKKIFSQHKGRYGVRRVYRELLRRGNSVNHKRVQRIMHSLGLQGKRPKEKYHSFKGEVGKVAPNIIDRDFTATAPLQ
KWTTDVSQFNFSWGKCYLSPIIDMYTNEVISYDLSMSPNLNQIKRMLEMAFKKFKSLTGLIFHSDQGWQYQHNYYRQELKNRGIIQSMSRRGNCIDNCIM
ETFFGRLKNEMYYSYEKEYESFESFSKALDEYISYYNNERIQGKTKWMPPVMYRIASMCST
Blast result :
Comments
ISStrsp1 is 62% aa similar to IS1221A.
The third ORF is the putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
The third ORF is the putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References