ISBli26

  • Family IS3
  • Group IS51
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
ND Brevibacterium linens
Brevibacterium linens
Brevibacterium linens ATCC 19391
DNA section
IS Length : 1315 bp

Ends


IR Length : 12/23

IRL : GCCCATAATGGGCCGGAGAATGCGAGTAGAAACCATGCCAAAGAAGAAGT
IRR : GGGCGTGAACCCCTGGAAGTGGACACATCAATGTGTACTATCAGGCGGCA

Comments : Atypical ends for an IS3 family member.

Insertion site


Left flankDirect repeatRight flankDR Length
CCTCAACTGCGAAGAATCAGACAGGACCAGCGA0

DNA sequence

GCCCATAATGGGCCGGAGAATGCGAGTAGAAACCATGCCAAAGAAGAAGTACTCCGATGAGTTCAAAGCCGACGCCGTCGCGCTGTACGAGACTCATCCC
GATATGTCTTATTCCCAAGTCGCGGCCGATCTTGGCATCTCCCGTGGCGCGATCAAGTCGTGGGTGTTCCAAGCGCGGAAGGAAGCTGGCAAGGTGTCCA
CGAGCTCGCCTGATCGGCCCGAGACACCCGAGGAGGAGCTTGCTCGCCTGCGGGTCGAGAACGATTCGTTGCGGGCCGATAAGCGGTCTCTGCGGTCGGA
TGTGGAGCGGCTGGAAGAAGAGCGAGCGATCCTGCGGAAAGCCACGAAATATTTCGCGGCAGAGACGACCTGGTGATCCGTTTCCAGTTCGTTGACGACC
ACCGAAACACCCATGAGGTGAAGCGGATGTGTTCCGTGCTGGGTATCCAGCGCTCAAGCTTTTATAAGTGGTGTGCCTCCCGGCCGGCACGGGCGGCCAG
GCAGGCTGCTGATGATGCTCTGTGCGAGCGGATTGAGCACCACTTCGAATTCTGGGACCACACGTACGGGTACCGACGGATCACGGCCGAACTCGCCGAT
GACCCGCACGTGGACGAGCCGGTCAACCGTAAGCGGGTTGCCCGCCTGATGCGGAACAACGGCATGGTCGGTATCCACTTGCGTAAGCCGAAGACTACGA
CGATCTCGGACCCAGATGCTCAGGTGTTTGCCGACCTGGTCGGCAGGGACTTCTCGGCCGCTGAGTTGGGCACGACCTACGTCGGCGACATCACCTACTT
ACCCTATGGCGATGAGGGGAAGTTCTTGTATTTGGCCACGGTCATCGACTTGTATTCGCGGCGGATCGTGGGCTGGTCGATCGCCGATCACATGCGGTCC
AGTCTCGTCGAGGATGCGTTACAGAATGCAGTAAACAACAGAGAAACACTGGAAGGTGCGATTTTTCACAGCGACCATGGTCGTCAATATACGTCCTCGT
CGTTTCAGGCGATGTGTCGTCGGTTCGGGGTGGTCCAGTCGATGGGCAGGGTCGGGTCGAGTGCTGATAATGCCCTGGCGGAGTCGGTCAACGCGGCGTT
GAAGCGGGAGACGCTGCAAGGAGCGAAGCGGTGGGTCTCGGACCTCGAGTGTCGTCGTGAGGTGTTTCGGTGGTTGGTTCGATACAACTCGAGGCGGAGA
CATTCGAGTCTGGGTTATCGAGCACCGGTTGACTTCGAGGTCGATTACACATTTATGATGGACATTGCCGCCTGATAGTACACATTGATGTGTCCACTTC
CAGGGGTTCACGCCC
Recoding section
  • Recoding by frameshift
  • Frame -1
  • Type translational
  • Experimentally demonstrated No

Stimulators :

  • Shine-Dalgarno sequence :
  • Secondary structure :

Recoding motif :

Protein section
ORF number : 3

 

ORF 1
LengthBeginEndStrandFusion ORF
342 bp113 aa35376+No
ORF function : Transposase
Description : First part of the transposase

ORF sequence :

MPKKKYSDEFKADAVALYETHPDMSYSQVAADLGISRGAIKSWVFQARKEAGKVSTSSPDRPETPEEELARLRVENDSLRADKRSLRSDVERLEEERAIL
RKATKYFAAETTW

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
993 bp330 aa2831275+No
ORF function : Transposase
Description : Second part of the transposase

ORF sequence :

AVSAVGCGAAGRRASDPAESHEIFRGRDDLVIRFQFVDDHRNTHEVKRMCSVLGIQRSSFYKWCASRPARAARQAADDALCERIEHHFEFWDHTYGYRRI
TAELADDPHVDEPVNRKRVARLMRNNGMVGIHLRKPKTTTISDPDAQVFADLVGRDFSAAELGTTYVIDLYSRRIVGWSIADHMRSVGDITYLPYGDEGK
FLYLATSLVEDALQNAVNNRETLEGAIFHSDHGRQYTSSSFQAMCRRFGVVQSMGRVGSSADNALAESVNAALKRETLQGAKRWVSDLECRREVFRWLVR
YNSRRRHSSLGYRAPVDFEVDYTFMMDIAA

 

Blast result :
ORF 3
LengthBeginEndStrandFusion ORF
1241 bp413 aa351275+Yes
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MPKKKYSDEFKADAVALYETHPDMSYSQVAADLGISRGAIKSWVFQARKEAGKVSTSSPDRPETPEEELARLRVENDSLRADKRSLRSDVERLEEERAIL
RKATKYFRGRDDLVIRFQFVDDHRNTHEVKRMCSVLGIQRSSFYKWCASRPARAARQAADDALCERIEHHFEFWDHTYGYRRITAELADDPHVDEPVNRK
RVARLMRNNGMVGIHLRKPKTTTISDPDAQVFADLVGRDFSAAELGTTYVIDLYSRRIVGWSIADHMRSVGDITYLPYGDEGKFLYLATSLVEDALQNAV
NNRETLEGAIFHSDHGRQYTSSSFQAMCRRFGVVQSMGRVGSSADNALAESVNAALKRETLQGAKRWVSDLECRREVFRWLVRYNSRRRHSSLGYRAPVD
FEVDYTFMMDIAA

 

Blast result :
Comments
ISBli26 is 59% aa similar to IS1206.
The third ORF is the putative ORFAB transposase re constructed in silico by possible -1 frameshift.
References
1] Sebastien Levesque (2017) Direct submission.