ISBli25
- Family IS3
- Group IS51
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| ND | Brevibacterium linens | Brevibacterium linens Brevibacterium linens ATCC 19391 |
DNA section
IS Length : 1328 bp
Ends
IR Length : 18/26
IRL : TGAATCGCCCCGGGGATAATAGAGGCAGGGAGATTGGAAGAAGGAGATTC
IRR : TGTCAAGCCCCGGGTTTCGTAGAGGCTCCTTTTCTTGGTTTCCTAAGCAG
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| CGAGGGTGGGGGTGGGTG | CTCTCTCCCCCGCGTGC | 0 | |
| GCCGCGGGCAATCCA | CAG | CTCTATGTCCACGCTGTCG | 3 |
DNA sequence
TGAATCGCCCCGGGGATAATAGAGGCAGGGAGATTGGAAGAAGGAGATTCTCTCTTGCCAGTGAAATACACCGACGAACTCAAGGCTCGTGCCGTCGAGC
TCGTCATCCATGCCCAGGCCGACCCCGACACCGCGAACGGAGCGATCACCCGTGTTGCGAACGAACTCGGTCTGAGTAAAGAAACCCTGCGAGTCTGGGT
GCGAAAGCACAAGGACTCCGGCAAAGCCACACCGGCCGAGTCGGTCGACCTGGAAGCGGAGAACCGTCGGCTCCGGGCTGAGCTGGCCGAAGCGAAGCGG
GCCAACGAGATTTTGAAGAGAGCGTCGGCTTTCTTCGCGGCGGAGCTCGACCGCCCATCGAAGTGATCGTCGCTTTCATCGACGATCATCGTCACGAGTT
CGGAGTCGAGCCAATCGTGCGCGCCCTGTCCGGGACTGCTGCACGGATTGCTGTGAGCTCGTATTACGCCTACAAGAAGCGTCAACCATCAGCCAGGGCC
CGTCGGGACCAAGCACTGATGGTGGTCATCCAGGATGTCTATGAGGCGAACTATTCCTGCTATGGAGTGCGGAAGATGTGGAAGGCGATCAACCGCGACT
ACGCCGACCAGTTCGGTCCGGTGGCCCGCTGCACAGTGGAGCGGTTGATGCGCCAGTTGGGCATTGACGGGATCCGGCGGAAGCGGAAGCGGCCGAAGAC
GGCCTCAGCACGTGCTGAGGAGTGTCCGGATGATCTCGTCGAACGTGAGTTCGCAGCACCGGCACCCAACTGTTTGTGGGTTGCGGACATCACGTATGTT
CCGACGTCTGCTGGGTGGGTGTACACGACGTTCATCCTTGATGTCTTCCACCGGGAGATCGTCGGCTGGCAGGTGACGAACCATATGCGGGAGTCGCTGG
CCAGGGACGCACTCACGATGGCTCTCGCTGCGAAGTATCGGGCCGGCGAAGACGTCTCCGGGCTGGTGCATCACTCGGATCGCGGGGTGCAATTCCGGTC
GATTCGCTATGGCGAGACCCTGGCGGAGTCCGAAATTGTGGCATCTGTGGGGTCGCGTGGGGACTCGTATGACAACGCGATGGCGGAGGCGCTGAACTCG
GTATACAAGGCCGAGCTTATCGACCGGAAAGTGTGGTCGGGGTTGATTGAGGTGATGGCGGAGACGTCGAAGTGGGTGGCTTGGTACAACCAGGAACGGC
TGCATTCAGCGACCGGGTACCGACCACCGTTCGAGGTCCATTCCGAATGGATCAACCAGTCTGCGACAGAGTCCGCGGCTGCTTAGGAAACCAAGAAAAG
GAGCCTCTACGAAACCCGGGGCTTGACA
TCGTCATCCATGCCCAGGCCGACCCCGACACCGCGAACGGAGCGATCACCCGTGTTGCGAACGAACTCGGTCTGAGTAAAGAAACCCTGCGAGTCTGGGT
GCGAAAGCACAAGGACTCCGGCAAAGCCACACCGGCCGAGTCGGTCGACCTGGAAGCGGAGAACCGTCGGCTCCGGGCTGAGCTGGCCGAAGCGAAGCGG
GCCAACGAGATTTTGAAGAGAGCGTCGGCTTTCTTCGCGGCGGAGCTCGACCGCCCATCGAAGTGATCGTCGCTTTCATCGACGATCATCGTCACGAGTT
CGGAGTCGAGCCAATCGTGCGCGCCCTGTCCGGGACTGCTGCACGGATTGCTGTGAGCTCGTATTACGCCTACAAGAAGCGTCAACCATCAGCCAGGGCC
CGTCGGGACCAAGCACTGATGGTGGTCATCCAGGATGTCTATGAGGCGAACTATTCCTGCTATGGAGTGCGGAAGATGTGGAAGGCGATCAACCGCGACT
ACGCCGACCAGTTCGGTCCGGTGGCCCGCTGCACAGTGGAGCGGTTGATGCGCCAGTTGGGCATTGACGGGATCCGGCGGAAGCGGAAGCGGCCGAAGAC
GGCCTCAGCACGTGCTGAGGAGTGTCCGGATGATCTCGTCGAACGTGAGTTCGCAGCACCGGCACCCAACTGTTTGTGGGTTGCGGACATCACGTATGTT
CCGACGTCTGCTGGGTGGGTGTACACGACGTTCATCCTTGATGTCTTCCACCGGGAGATCGTCGGCTGGCAGGTGACGAACCATATGCGGGAGTCGCTGG
CCAGGGACGCACTCACGATGGCTCTCGCTGCGAAGTATCGGGCCGGCGAAGACGTCTCCGGGCTGGTGCATCACTCGGATCGCGGGGTGCAATTCCGGTC
GATTCGCTATGGCGAGACCCTGGCGGAGTCCGAAATTGTGGCATCTGTGGGGTCGCGTGGGGACTCGTATGACAACGCGATGGCGGAGGCGCTGAACTCG
GTATACAAGGCCGAGCTTATCGACCGGAAAGTGTGGTCGGGGTTGATTGAGGTGATGGCGGAGACGTCGAAGTGGGTGGCTTGGTACAACCAGGAACGGC
TGCATTCAGCGACCGGGTACCGACCACCGTTCGAGGTCCATTCCGAATGGATCAACCAGTCTGCGACAGAGTCCGCGGCTGCTTAGGAAACCAAGAAAAG
GAGCCTCTACGAAACCCGGGGCTTGACA
Recoding section
- Recoding by frameshift
- Frame -1
- Type translational
- Experimentally demonstrated No
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 306 bp | 101 aa | 61 | 366 | + | No |
Description : First part of the transposase
ORF sequence :
VKYTDELKARAVELVIHAQADPDTANGAITRVANELGLSKETLRVWVRKHKDSGKATPAESVDLEAENRRLRAELAEAKRANEILKRASAFFAAELDRPS
K
K
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1004 bp | 334 aa | 282 | 1285 | + | No |
Description : Second part of the transposase
ORF sequence :
AGRSEAGQRDFEESVGFLRGGARPPIEVIVAFIDDHRHEFGVEPIVRALSGTAARIAVSSYYAYKKRQPSARARRDQALMVVIQDVYEANYSCYGVRKMW
KAINRDYADQFGPVARCTVERLMRQLGIDGIRRKRKRPKTASARAEECPDDLVEREFAAPAPNCLWVADITYVPTSAGWVYTTFILDVFHREIVGWQVTN
HMRESLARDALTMALAAKYRAGEDVSGLVHHSDRGVQFRSIRYGETLAESEIVASVGSRGDSYDNAMAEALNSVYKAELIDRKVWSGLIEVMAETSKWVA
WYNQERLHSATGYRPPFEVHSEWINQSATESAAA
KAINRDYADQFGPVARCTVERLMRQLGIDGIRRKRKRPKTASARAEECPDDLVEREFAAPAPNCLWVADITYVPTSAGWVYTTFILDVFHREIVGWQVTN
HMRESLARDALTMALAAKYRAGEDVSGLVHHSDRGVQFRSIRYGETLAESEIVASVGSRGDSYDNAMAEALNSVYKAELIDRKVWSGLIEVMAETSKWVA
WYNQERLHSATGYRPPFEVHSEWINQSATESAAA
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1225 bp | 408 aa | 61 | 1285 | + | Yes |
Chemistry : DDE
ORF sequence :
VKYTDELKARAVELVIHAQADPDTANGAITRVANELGLSKETLRVWVRKHKDSGKATPAESVDLEAENRRLRAELAEAKRANEILKRASAFLRGGARPPI
EVIVAFIDDHRHEFGVEPIVRALSGTAARIAVSSYYAYKKRQPSARARRDQALMVVIQDVYEANYSCYGVRKMWKAINRDYADQFGPVARCTVERLMRQL
GIDGIRRKRKRPKTASARAEECPDDLVEREFAAPAPNCLWVADITYVPTSAGWVYTTFILDVFHREIVGWQVTNHMRESLARDALTMALAAKYRAGEDVS
GLVHHSDRGVQFRSIRYGETLAESEIVASVGSRGDSYDNAMAEALNSVYKAELIDRKVWSGLIEVMAETSKWVAWYNQERLHSATGYRPPFEVHSEWINQ
SATESAAA
EVIVAFIDDHRHEFGVEPIVRALSGTAARIAVSSYYAYKKRQPSARARRDQALMVVIQDVYEANYSCYGVRKMWKAINRDYADQFGPVARCTVERLMRQL
GIDGIRRKRKRPKTASARAEECPDDLVEREFAAPAPNCLWVADITYVPTSAGWVYTTFILDVFHREIVGWQVTNHMRESLARDALTMALAAKYRAGEDVS
GLVHHSDRGVQFRSIRYGETLAESEIVASVGSRGDSYDNAMAEALNSVYKAELIDRKVWSGLIEVMAETSKWVAWYNQERLHSATGYRPPFEVHSEWINQ
SATESAAA
Blast result :
Comments
ISBli25 is 61% aa similar to ISPmi1.
The third ORF is the putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
There are two copies in the genome, the second is inserted into a copy of ISBli23 (IS481 family mmeber).
The third ORF is the putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
There are two copies in the genome, the second is inserted into a copy of ISBli23 (IS481 family mmeber).
References
1] Sebastien Levesque (2017) Direct submission.