ISPa82

  • Family IS66
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
ND Pseudomonas aeruginosa
Pseudomonas aeruginosa
DNA section
IS Length : 2017 bp

Ends


IR Length : 19/25

IRL : GTATGCGCTCCATAGACCCCATATTCAACTGACCTCTCCGTATCCCGGAT
IRR : GTAACCGCTCCATAAACCCCGTCCTGGCGGACGGGTTTTTTCAGGAAGCA

Insertion site


Left flankDirect repeatRight flankDR Length
AG0

DNA sequence

GTATGCGCTCCATAGACCCCATATTCAACTGACCTCTCCGTATCCCGGATGCTGGGCTCCCTATTTTCCCGTGGAGTTCCGTCATGATGCGTCCCGACGC
CAAAGTGCAGAAGGTCTATCTCTACCCAAAGCCCGTCGACTTCCGCAAATCCATCAATGGACTGGCCGCGCTGGTCGAGCTGGACATCAAGGTGGAGGTG
TTCAACCCGGTGCTGTTCGTGTTCCTCAATCGCACGCGCAGCCAGGTCAAGATTCTCTACTGGGAGCGCAATGGCTTCTGCCTGTGGCTCAAGCGACTGG
AAGCCGAGCGTTTCAAGACCAAACCCGATGCCGGTGACGAAGCCATCGAACTGACGGTCGACGAACTGAACTGGCTGCTCGACGGTATCGACCTGTGGCG
TAACCGTCCGCATCAGGTTCTGACGCCGCGTTACGTGGCCTGAGCCGGTATAATCCGGCGCCATGAAATTTGGCGCCGACAACCTTCCCGACGATCCCGT
CCTGCTCAAGGCTCTGATTCTGCAGTTGCAGGAAGAGAACGCCTTGCTGCGCCACAAGCTGTTCTCGCCCAAGTCCGAGCGCAGCCCTGAAGACGCCGAC
TCGCCGCAGTTGGCCATGTTCAACGAGGTCGAAGAGCTGATCGAAGCGGCCGCTGCGCCAAGCGAAGCCGAAGCCGAAGCCAAAGAAGTCGTTGCGCCGG
TCAAGCGCCGTGGCAAACGCAAGCCGCTGCCGGCCAACCTGCCGCGTATGGAGGTCATCCATGAGCTGCCCGAGCACGAGCGCACCTGCGCCTGCGGTTC
CTGCAAGCAGGTGATCGGCGAGGAGACCAGCGAGCAGCTGGAGATCATCCCGATGCAGGTGCGGGTCATCCGCCACATCCGCAAGACCTATGCCTGCAAG
GCCTGTGAAACCGCGCCGGTCACCGCCGATAAACCGGCACAACTGATCGAGAAGAGCCTGGCCAGCCCCAGCGTGCTGGCGATGCTGCTGACCACCAAGT
ACGCCGACGGCATTCCGCTGTACCGCTTCGAAAAGATGCTCAGCCGCCACGGCGTCGACATCCCACGGCAGACCCTGGCGCGTTGGGTGATCCAAAGTGG
CGAGCAACTGCAACCGCTGCTCAACCTGATGCGCGACAAGCTGCTGGACTACCCCGTGCTGCACTGCGACGAAACGCGCTTGCAGGTGCTGCATGAACCG
GGGCGCGACCCCTCGGCACAATCCTGGATGTGGGTGCAAAGCGGTGGGCCGCCGGACAAGCCGGTGATCCTTTTCGACTACACAGCCAGCCGCGCGCAGG
AGGTGCCGCTACGCCTGCTCGATGGCTACCGCGGCTACCTGATGACCGACGACTACGCCGGCTACAACGCCGTGGCTGCACGCGACGGCATCGAACGCCT
GGGCTGCTGGGCGCATGCGCGACGCAAGTTCGTCGAAGCCCAAAAGGTGCAGCCCAAGGGCAAGACTGGGCGTGCCGACGTGGCGCTGAGCCTGATCAAC
AAGCTCTACGGCATCGAGCGTGACCTGAAGGACGCTGACCATAGCGAACGCCTCGACGTCCGCCAGCAACGCAGCCAGCCCCTGATCGACCAACTCAAGG
CCTGGCTGGACAAAACCCAGCCGCAGGTCGCTGGGCAGACGGCCCTGGGCAAGGCGGTAAACTACTTGGCCAGAAACTGGAGCAGGCTGGTGCGCTACAT
CGAAGGCGGGCATCTACCGATCGACAACAACCGCGCCGAGAACGCCATCCGTCCCTTCGTCATCGGCCGCAAGAACTGGCTGTTCAGCGACACGCCGAAA
GGCGCTACCGCCAGCGCGCAGATCTACAGCCTGATCGAAACTGCCAAAGCCAACGGGCAAGAGCCTTACGCCTGGTTGCGCCACATCCTTGAACGCCTGC
CGACTGCCAGTAGCGTCGAGGATTACGAAGCGCTGCTGCCGTGGAACAGCTCGCCAGCTTCTGCCTCTGCTTCCTGAAAAAACCCGTCCGCCAGGACGGG
GTTTATGGAGCGGTTAC
Protein section
ORF number : 2

 

ORF 1
LengthBeginEndStrandFusion ORF
360 bp119 aa84443+No
ORF function : Accessory Gene
AG : IS66 TnpA

ORF sequence :

MMRPDAKVQKVYLYPKPVDFRKSINGLAALVELDIKVEVFNPVLFVFLNRTRSQVKILYWERNGFCLWLKRLEAERFKTKPDAGDEAIELTVDELNWLLD
GIDLWRNRPHQVLTPRYVA

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
1515 bp504 aa4631977+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MKFGADNLPDDPVLLKALILQLQEENALLRHKLFSPKSERSPEDADSPQLAMFNEVEELIEAAAAPSEAEAEAKEVVAPVKRRGKRKPLPANLPRMEVIH
ELPEHERTCACGSCKQVIGEETSEQLEIIPMQVRVIRHIRKTYACKACETAPVTADKPAQLIEKSLASPSVLAMLLTTKYADGIPLYRFEKMLSRHGVDI
PRQTLARWVIQSGEQLQPLLNLMRDKLLDYPVLHCDETRLQVLHEPGRDPSAQSWMWVQSGGPPDKPVILFDYTASRAQEVPLRLLDGYRGYLMTDDYAG
YNAVAARDGIERLGCWAHARRKFVEAQKVQPKGKTGRADVALSLINKLYGIERDLKDADHSERLDVRQQRSQPLIDQLKAWLDKTQPQVAGQTALGKAVN
YLARNWSRLVRYIEGGHLPIDNNRAENAIRPFVIGRKNWLFSDTPKGATASAQIYSLIETAKANGQEPYAWLRHILERLPTASSVEDYEALLPWNSSPAS
ASAS

 

Blast result :
Comments
ISPa82 is 84% (ORF2 : the transposase) aa similar to ISPpu13.
References
1] Dongsheng Zhou (2018) Direct submission.