ISPa79

  • Family IS3
  • Group IS150
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
LT969520 ND Pseudomonas aeruginosa
Pseudomonas aeruginosa RW109
Pseudomonas aeruginosa p1160
DNA section
IS Length : 1473 bp

Ends


IR Length : 35/41

IRL : TGGTGTCCACCTATTTATGATCTGACCCCGAAATGTTGGACATCAACATT
IRR : TGGTGTCCACTTAATGATCTGACCCCGAAATGTTGGACGCCTTCTATGCC

Insertion site


Left flankDirect repeatRight flankDR Length
CGCCAAAGGCGACTTCTAGCCTT0

DNA sequence

TGGTGTCCACCTATTTATGATCTGACCCCGAAATGTTGGACATCAACATTGGGGGGTGTCATGAAAAAATACACAGAGCAGACGAAATTGTCGGTTGTTG
AAGATTACTGCTCGGGGAGCGCAGGTCACAGAGAGGTCGCGCATCGTCATGGGGTAGATGCTTCATCGCTTCGAAAGTGGATCGCGGCATATCAGGCGCT
TGGTGCCGCAGGCCTCAAAAGTAAGAGAAAGAAGAACTACAGCCCTGAGTTCAAGCTGAATGTTTTGCAGCGCATGCGAGAGGAAGAGCTGTCCTATCGC
CAAGTCGCTGCCCTGTTTGATATCCGGAAATCCGACATCATTGGCGAATGGGAGCGCAGGTATGATGAAGGCGGTTTGGAGGCCTTGTCCCGGCAGCCAG
GAAGCGGACTTCACAAAAAAATGACAAACCCCATCCCGTCCATTCAGCTTGAATCTTCGGACGATGAAGCTCGTACGCGCGATGATTTGCTGGCCGAATT
GAGCCAGTTGCGGATGGAAATTGCTTATTTAAAAAAGCTCGATGCCTTGGTTCAAGCGAAGGAACGAGCAGCGCAGCGGAAAAAGCGCAAATCGTGATTG
AACTGAGGCAGGAGCACCCTCTGGAAGGCCTTCTGAAATTGGCTGGCCTGGCCCGTAGCACTTTTTACTATCAACAAAAGGTGCAGCAATCCGTTGATAA
ATACGCGGTGCTGAAGTCCAGAATTTGCTCAATTTTCGACGAGCACAAGGGCCGATATGGCTATCGCCGTATCACTGCTGCCGTTCGGCGAGCTGGCCAC
CTCGTGAACCACAAGACTGTTCAGCGCCTCATGGGCGAGCTTCAGTTGAAAAGCCGTGTGCGCATAAAGAAATACCGGGCCTACAGAGGCGAAGAGGGTC
AGGTAGCGCCGAACCTGCTGAAACGGAAGTTCGATGCCAAACGACCGAACCAGAAATGGGTAACCGATGTGACGGAGTTTCGGGTCGGGGGGCAGAAGCT
TTATCTCTCGCCGATCCTTGACCTCTATAACGGCGAAATAATCGCCTTCAAGACCGCGAGGCGCCCGCTGTTCGACATGGTTGGTGCGATGCTACGGCAA
GCTTTTGAGAGGCTGCGGCCACGCGATAAACCAATTTTGCATTCCGACCAGGGATGGCAGTACCGGATGCCTATCTACCGCCGCGTGCTGAACGAGCGTG
CGGTAAAGCCAAGCATGTCCCGCAAAGGAAATTGCTATGACAATGCGGCTATGGAAAGCTTTTTTGGCACGTTGAAATCCGAGTTCTTTTACCTGAACAA
ATTCAACAGTCTCGATGAGCTTGAAGCAGGTATAGAGGACTACATACACTACTACAATCATTCGCGAATCAGGCTGAAGCTAAGTGGCCTGAGCCCTGTG
GAATACAGGACTCAAGCACGGCGGGCATAGAAGGCGTCCAACATTTCGGGGTCAGATCATTAAGTGGACACCA
Recoding section
  • Recoding by frameshift
  • Frame -1
  • Type translational
  • Experimentally demonstrated No

Stimulators :

  • Shine-Dalgarno sequence :
  • Secondary structure :

Recoding motif :

Protein section
ORF number : 3

 

ORF 1
LengthBeginEndStrandFusion ORF
537 bp178 aa61597+No
ORF function : Transposase
Description : First part of the transposase

ORF sequence :

MKKYTEQTKLSVVEDYCSGSAGHREVAHRHGVDASSLRKWIAAYQALGAAGLKSKRKKNYSPEFKLNVLQRMREEELSYRQVAALFDIRKSDIIGEWERR
YDEGGLEALSRQPGSGLHKKMTNPIPSIQLESSDDEARTRDDLLAELSQLRMEIAYLKKLDALVQAKERAAQRKKRKS

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
945 bp314 aa4861430+No
ORF function : Transposase
Description : Second part of the transposase

ORF sequence :

FAGRIEPVADGNCLFKKARCLGSSEGTSSAAEKAQIVIELRQEHPLEGLLKLAGLARSTFYYQQKVQQSVDKYAVLKSRICSIFDEHKGRYGYRRITAAV
RRAGHLVNHKTVQRLMGELQLKSRVRIKKYRAYRGEEGQVAPNLLKRKFDAKRPNQKWVTDVTEFRVGGQKLYLSPILDLYNGEIIAFKTARRPLFDMVG
AMLRQAFERLRPRDKPILHSDQGWQYRMPIYRRVLNERAVKPSMSRKGNCYDNAAMESFFGTLKSEFFYLNKFNSLDELEAGIEDYIHYYNHSRIRLKLS
GLSPVEYRTQARRA

 

Blast result :
ORF 3
LengthBeginEndStrandFusion ORF
1370 bp456 aa611430+Yes
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MKKYTEQTKLSVVEDYCSGSAGHREVAHRHGVDASSLRKWIAAYQALGAAGLKSKRKKNYSPEFKLNVLQRMREEELSYRQVAALFDIRKSDIIGEWERR
YDEGGLEALSRQPGSGLHKKMTNPIPSIQLESSDDEARTRDDLLAELSQLRMEIAYLKKARCLGSSEGTSSAAEKAQIVIELRQEHPLEGLLKLAGLARS
TFYYQQKVQQSVDKYAVLKSRICSIFDEHKGRYGYRRITAAVRRAGHLVNHKTVQRLMGELQLKSRVRIKKYRAYRGEEGQVAPNLLKRKFDAKRPNQKW
VTDVTEFRVGGQKLYLSPILDLYNGEIIAFKTARRPLFDMVGAMLRQAFERLRPRDKPILHSDQGWQYRMPIYRRVLNERAVKPSMSRKGNCYDNAAMES
FFGTLKSEFFYLNKFNSLDELEAGIEDYIHYYNHSRIRLKLSGLSPVEYRTQARRA

 

Blast result :
Comments
ISPa79 is 81% aa similar to ISPsy9.
The third ORF is the putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References
1] Dongsheng Zhou (2017) Direct submission.
2] Zeng,L., Zhan,Z., Liang,L., Feng,J., Zhao,Y., Luo,W., Jiang,X. and Zhou,D. (2017) Direct GenBank submission.
3] Green,A. (2017) Direct GenBank submission.