ISPa79
- Family IS3
- Group IS150
Isoform Synonym(s)
Accession number | Transposition | Origin | Host |
---|---|---|---|
LT969520 | ND | Pseudomonas aeruginosa | Pseudomonas aeruginosa RW109 Pseudomonas aeruginosa p1160 |
DNA section
IS Length : 1473 bp
Ends
IR Length : 35/41
IRL : TGGTGTCCACCTATTTATGATCTGACCCCGAAATGTTGGACATCAACATT
IRR : TGGTGTCCACTTAATGATCTGACCCCGAAATGTTGGACGCCTTCTATGCC
Insertion site
Left flank | Direct repeat | Right flank | DR Length |
---|---|---|---|
CGCCAAAGGCGA | CTTCTAGCCTT | 0 |
DNA sequence
TGGTGTCCACCTATTTATGATCTGACCCCGAAATGTTGGACATCAACATTGGGGGGTGTCATGAAAAAATACACAGAGCAGACGAAATTGTCGGTTGTTG
AAGATTACTGCTCGGGGAGCGCAGGTCACAGAGAGGTCGCGCATCGTCATGGGGTAGATGCTTCATCGCTTCGAAAGTGGATCGCGGCATATCAGGCGCT
TGGTGCCGCAGGCCTCAAAAGTAAGAGAAAGAAGAACTACAGCCCTGAGTTCAAGCTGAATGTTTTGCAGCGCATGCGAGAGGAAGAGCTGTCCTATCGC
CAAGTCGCTGCCCTGTTTGATATCCGGAAATCCGACATCATTGGCGAATGGGAGCGCAGGTATGATGAAGGCGGTTTGGAGGCCTTGTCCCGGCAGCCAG
GAAGCGGACTTCACAAAAAAATGACAAACCCCATCCCGTCCATTCAGCTTGAATCTTCGGACGATGAAGCTCGTACGCGCGATGATTTGCTGGCCGAATT
GAGCCAGTTGCGGATGGAAATTGCTTATTTAAAAAAGCTCGATGCCTTGGTTCAAGCGAAGGAACGAGCAGCGCAGCGGAAAAAGCGCAAATCGTGATTG
AACTGAGGCAGGAGCACCCTCTGGAAGGCCTTCTGAAATTGGCTGGCCTGGCCCGTAGCACTTTTTACTATCAACAAAAGGTGCAGCAATCCGTTGATAA
ATACGCGGTGCTGAAGTCCAGAATTTGCTCAATTTTCGACGAGCACAAGGGCCGATATGGCTATCGCCGTATCACTGCTGCCGTTCGGCGAGCTGGCCAC
CTCGTGAACCACAAGACTGTTCAGCGCCTCATGGGCGAGCTTCAGTTGAAAAGCCGTGTGCGCATAAAGAAATACCGGGCCTACAGAGGCGAAGAGGGTC
AGGTAGCGCCGAACCTGCTGAAACGGAAGTTCGATGCCAAACGACCGAACCAGAAATGGGTAACCGATGTGACGGAGTTTCGGGTCGGGGGGCAGAAGCT
TTATCTCTCGCCGATCCTTGACCTCTATAACGGCGAAATAATCGCCTTCAAGACCGCGAGGCGCCCGCTGTTCGACATGGTTGGTGCGATGCTACGGCAA
GCTTTTGAGAGGCTGCGGCCACGCGATAAACCAATTTTGCATTCCGACCAGGGATGGCAGTACCGGATGCCTATCTACCGCCGCGTGCTGAACGAGCGTG
CGGTAAAGCCAAGCATGTCCCGCAAAGGAAATTGCTATGACAATGCGGCTATGGAAAGCTTTTTTGGCACGTTGAAATCCGAGTTCTTTTACCTGAACAA
ATTCAACAGTCTCGATGAGCTTGAAGCAGGTATAGAGGACTACATACACTACTACAATCATTCGCGAATCAGGCTGAAGCTAAGTGGCCTGAGCCCTGTG
GAATACAGGACTCAAGCACGGCGGGCATAGAAGGCGTCCAACATTTCGGGGTCAGATCATTAAGTGGACACCA
AAGATTACTGCTCGGGGAGCGCAGGTCACAGAGAGGTCGCGCATCGTCATGGGGTAGATGCTTCATCGCTTCGAAAGTGGATCGCGGCATATCAGGCGCT
TGGTGCCGCAGGCCTCAAAAGTAAGAGAAAGAAGAACTACAGCCCTGAGTTCAAGCTGAATGTTTTGCAGCGCATGCGAGAGGAAGAGCTGTCCTATCGC
CAAGTCGCTGCCCTGTTTGATATCCGGAAATCCGACATCATTGGCGAATGGGAGCGCAGGTATGATGAAGGCGGTTTGGAGGCCTTGTCCCGGCAGCCAG
GAAGCGGACTTCACAAAAAAATGACAAACCCCATCCCGTCCATTCAGCTTGAATCTTCGGACGATGAAGCTCGTACGCGCGATGATTTGCTGGCCGAATT
GAGCCAGTTGCGGATGGAAATTGCTTATTTAAAAAAGCTCGATGCCTTGGTTCAAGCGAAGGAACGAGCAGCGCAGCGGAAAAAGCGCAAATCGTGATTG
AACTGAGGCAGGAGCACCCTCTGGAAGGCCTTCTGAAATTGGCTGGCCTGGCCCGTAGCACTTTTTACTATCAACAAAAGGTGCAGCAATCCGTTGATAA
ATACGCGGTGCTGAAGTCCAGAATTTGCTCAATTTTCGACGAGCACAAGGGCCGATATGGCTATCGCCGTATCACTGCTGCCGTTCGGCGAGCTGGCCAC
CTCGTGAACCACAAGACTGTTCAGCGCCTCATGGGCGAGCTTCAGTTGAAAAGCCGTGTGCGCATAAAGAAATACCGGGCCTACAGAGGCGAAGAGGGTC
AGGTAGCGCCGAACCTGCTGAAACGGAAGTTCGATGCCAAACGACCGAACCAGAAATGGGTAACCGATGTGACGGAGTTTCGGGTCGGGGGGCAGAAGCT
TTATCTCTCGCCGATCCTTGACCTCTATAACGGCGAAATAATCGCCTTCAAGACCGCGAGGCGCCCGCTGTTCGACATGGTTGGTGCGATGCTACGGCAA
GCTTTTGAGAGGCTGCGGCCACGCGATAAACCAATTTTGCATTCCGACCAGGGATGGCAGTACCGGATGCCTATCTACCGCCGCGTGCTGAACGAGCGTG
CGGTAAAGCCAAGCATGTCCCGCAAAGGAAATTGCTATGACAATGCGGCTATGGAAAGCTTTTTTGGCACGTTGAAATCCGAGTTCTTTTACCTGAACAA
ATTCAACAGTCTCGATGAGCTTGAAGCAGGTATAGAGGACTACATACACTACTACAATCATTCGCGAATCAGGCTGAAGCTAAGTGGCCTGAGCCCTGTG
GAATACAGGACTCAAGCACGGCGGGCATAGAAGGCGTCCAACATTTCGGGGTCAGATCATTAAGTGGACACCA
Recoding section
- Recoding by frameshift
- Frame -1
- Type translational
- Experimentally demonstrated No
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
Length | Begin | End | Strand | Fusion ORF | |
---|---|---|---|---|---|
537 bp | 178 aa | 61 | 597 | + | No |
Description : First part of the transposase
ORF sequence :
MKKYTEQTKLSVVEDYCSGSAGHREVAHRHGVDASSLRKWIAAYQALGAAGLKSKRKKNYSPEFKLNVLQRMREEELSYRQVAALFDIRKSDIIGEWERR
YDEGGLEALSRQPGSGLHKKMTNPIPSIQLESSDDEARTRDDLLAELSQLRMEIAYLKKLDALVQAKERAAQRKKRKS
YDEGGLEALSRQPGSGLHKKMTNPIPSIQLESSDDEARTRDDLLAELSQLRMEIAYLKKLDALVQAKERAAQRKKRKS
Blast result :ORF 2
Length | Begin | End | Strand | Fusion ORF | |
---|---|---|---|---|---|
945 bp | 314 aa | 486 | 1430 | + | No |
Description : Second part of the transposase
ORF sequence :
FAGRIEPVADGNCLFKKARCLGSSEGTSSAAEKAQIVIELRQEHPLEGLLKLAGLARSTFYYQQKVQQSVDKYAVLKSRICSIFDEHKGRYGYRRITAAV
RRAGHLVNHKTVQRLMGELQLKSRVRIKKYRAYRGEEGQVAPNLLKRKFDAKRPNQKWVTDVTEFRVGGQKLYLSPILDLYNGEIIAFKTARRPLFDMVG
AMLRQAFERLRPRDKPILHSDQGWQYRMPIYRRVLNERAVKPSMSRKGNCYDNAAMESFFGTLKSEFFYLNKFNSLDELEAGIEDYIHYYNHSRIRLKLS
GLSPVEYRTQARRA
RRAGHLVNHKTVQRLMGELQLKSRVRIKKYRAYRGEEGQVAPNLLKRKFDAKRPNQKWVTDVTEFRVGGQKLYLSPILDLYNGEIIAFKTARRPLFDMVG
AMLRQAFERLRPRDKPILHSDQGWQYRMPIYRRVLNERAVKPSMSRKGNCYDNAAMESFFGTLKSEFFYLNKFNSLDELEAGIEDYIHYYNHSRIRLKLS
GLSPVEYRTQARRA
Blast result :ORF 3
Length | Begin | End | Strand | Fusion ORF | |
---|---|---|---|---|---|
1370 bp | 456 aa | 61 | 1430 | + | Yes |
Chemistry : DDE
ORF sequence :
MKKYTEQTKLSVVEDYCSGSAGHREVAHRHGVDASSLRKWIAAYQALGAAGLKSKRKKNYSPEFKLNVLQRMREEELSYRQVAALFDIRKSDIIGEWERR
YDEGGLEALSRQPGSGLHKKMTNPIPSIQLESSDDEARTRDDLLAELSQLRMEIAYLKKARCLGSSEGTSSAAEKAQIVIELRQEHPLEGLLKLAGLARS
TFYYQQKVQQSVDKYAVLKSRICSIFDEHKGRYGYRRITAAVRRAGHLVNHKTVQRLMGELQLKSRVRIKKYRAYRGEEGQVAPNLLKRKFDAKRPNQKW
VTDVTEFRVGGQKLYLSPILDLYNGEIIAFKTARRPLFDMVGAMLRQAFERLRPRDKPILHSDQGWQYRMPIYRRVLNERAVKPSMSRKGNCYDNAAMES
FFGTLKSEFFYLNKFNSLDELEAGIEDYIHYYNHSRIRLKLSGLSPVEYRTQARRA
YDEGGLEALSRQPGSGLHKKMTNPIPSIQLESSDDEARTRDDLLAELSQLRMEIAYLKKARCLGSSEGTSSAAEKAQIVIELRQEHPLEGLLKLAGLARS
TFYYQQKVQQSVDKYAVLKSRICSIFDEHKGRYGYRRITAAVRRAGHLVNHKTVQRLMGELQLKSRVRIKKYRAYRGEEGQVAPNLLKRKFDAKRPNQKW
VTDVTEFRVGGQKLYLSPILDLYNGEIIAFKTARRPLFDMVGAMLRQAFERLRPRDKPILHSDQGWQYRMPIYRRVLNERAVKPSMSRKGNCYDNAAMES
FFGTLKSEFFYLNKFNSLDELEAGIEDYIHYYNHSRIRLKLSGLSPVEYRTQARRA
Blast result :
Comments
ISPa79 is 81% aa similar to ISPsy9.
The third ORF is the putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
The third ORF is the putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References
1] Dongsheng Zhou (2017) Direct submission.
2] Zeng,L., Zhan,Z., Liang,L., Feng,J., Zhao,Y., Luo,W., Jiang,X. and Zhou,D. (2017) Direct GenBank submission.
3] Green,A. (2017) Direct GenBank submission.
2] Zeng,L., Zhan,Z., Liang,L., Feng,J., Zhao,Y., Luo,W., Jiang,X. and Zhou,D. (2017) Direct GenBank submission.
3] Green,A. (2017) Direct GenBank submission.