ISSsu11
- Family IS3
- Group IS150
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| CP006246 | ND | Streptococcus suis | Streptococcus suis T15 |
DNA section
IS Length : 1311 bp
Ends
IR Length : 31/39
IRL : TGAAAAGAACCGCTTATAATGGACAGTATAAAAAAGTTATATACTGTTCA
IRR : TGTCAAGCACCAGATAAAATGGACAGTTAAAAAAAGTTAGTTTTTTATAA
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| AAAAAGGGAGTGGAC | GTCAGAACTCGACC | 0 |
DNA sequence
TGAAAAGAACCGCTTATAATGGACAGTATAAAAAAGTTATATACTGTTCATATAAGGAGGTTCTTTTTTCATGGCTAAAAAAGGAAGCAAGTTCACCAAA
TATCCACCCGAGTTCAAACTACAGGTAGTTGAAGACTACCTCAGCGGAAAGAGCGGAGGGATGCCTTCTATTGTCAAAAAATATGGGTTAAAATCAGACA
ACCAAGTTTTAACTTGGACAAGAAAATACCGAGAAGACCCTACTTTAATCTCCCAAGATTTACGAGGTAGAAAGTCCACTGGTCGTCCCAAAACAAGGAA
TCTAGAAGAGATGACCCTTGAGGAACAGAATACTTATCTACGAATGGAGAATGCAATTTTAAAGACCTTAAGGACTCTCCTCAATCCGTGAGAAGAGTCC
ACCTCTATCAAGTCGTGGAGCAGTTGAAAGATCGTTACTCTGTCTCCTCTTTATGCGCCTATTTCGGCTTCCCCAGGTCTTCTTACTACCTTTGGTTGAG
CAAGGGGAGACCGGTCTATAAACAGTTTGATGCAAACTTAGCCCAAAAGATTACAGCTATCTTCCATGACACTCAAAAAGGCTATCGCTTCATCTCTTAT
CAACTGAGAAGACACTATCAACTTTCTCCTAACCCTAAGACTGTCTTGCGTTATATGCGTATTCTTGGTCACCAGTCTCCGATTCGGAAGAAGCGGTATC
AATCCTGCACGCAACGAGAGATAAATGAAAAGGCAAGACACGTCCACTACAACGTGCTTGTCCGAAACTTTAAAGCAGAACGTCCTCTCCAAAAACTGAC
GACGGATGTCAGCTACGTTTACCACAAACAAGGAAGGATGTTCTTAAGTGTCATAAAAGATTGTTATGACAATTCCATTTTAGCCTATACGCTATCAGAT
TTCAATGATAACAAGTTAGTTTTTGACAATATAGACCTGGTTTTTCACGATTCTTGGGATTCAACCAAAGTCTGTATCCTTCATTCTGACCAAGGTTTTC
AATATACCAATCAACAGTATCTCCGAAAATTAGACCAATATGGTGTGACCATATCTCATTCTGGCAAGGGAAATTGTTATGATAATGCGGCATGTGAGAA
TTTCTTTTCCCACTTGAAGAGTGAATCCTTGAGACTATTTCCGCCTGAGGATAGGGAAGCCCTCATCCAACAAATCAGGGAATACATCGACTGGTATAAT
TGGCACAGACCACAAGAAAAATTAAAAGGTATGACTCCCTTAGAATTTAGGGAAGCATACCTTATAAAAAACTAACTTTTTTTAACTGTCCATTTTATCT
GGTGCTTGACA
TATCCACCCGAGTTCAAACTACAGGTAGTTGAAGACTACCTCAGCGGAAAGAGCGGAGGGATGCCTTCTATTGTCAAAAAATATGGGTTAAAATCAGACA
ACCAAGTTTTAACTTGGACAAGAAAATACCGAGAAGACCCTACTTTAATCTCCCAAGATTTACGAGGTAGAAAGTCCACTGGTCGTCCCAAAACAAGGAA
TCTAGAAGAGATGACCCTTGAGGAACAGAATACTTATCTACGAATGGAGAATGCAATTTTAAAGACCTTAAGGACTCTCCTCAATCCGTGAGAAGAGTCC
ACCTCTATCAAGTCGTGGAGCAGTTGAAAGATCGTTACTCTGTCTCCTCTTTATGCGCCTATTTCGGCTTCCCCAGGTCTTCTTACTACCTTTGGTTGAG
CAAGGGGAGACCGGTCTATAAACAGTTTGATGCAAACTTAGCCCAAAAGATTACAGCTATCTTCCATGACACTCAAAAAGGCTATCGCTTCATCTCTTAT
CAACTGAGAAGACACTATCAACTTTCTCCTAACCCTAAGACTGTCTTGCGTTATATGCGTATTCTTGGTCACCAGTCTCCGATTCGGAAGAAGCGGTATC
AATCCTGCACGCAACGAGAGATAAATGAAAAGGCAAGACACGTCCACTACAACGTGCTTGTCCGAAACTTTAAAGCAGAACGTCCTCTCCAAAAACTGAC
GACGGATGTCAGCTACGTTTACCACAAACAAGGAAGGATGTTCTTAAGTGTCATAAAAGATTGTTATGACAATTCCATTTTAGCCTATACGCTATCAGAT
TTCAATGATAACAAGTTAGTTTTTGACAATATAGACCTGGTTTTTCACGATTCTTGGGATTCAACCAAAGTCTGTATCCTTCATTCTGACCAAGGTTTTC
AATATACCAATCAACAGTATCTCCGAAAATTAGACCAATATGGTGTGACCATATCTCATTCTGGCAAGGGAAATTGTTATGATAATGCGGCATGTGAGAA
TTTCTTTTCCCACTTGAAGAGTGAATCCTTGAGACTATTTCCGCCTGAGGATAGGGAAGCCCTCATCCAACAAATCAGGGAATACATCGACTGGTATAAT
TGGCACAGACCACAAGAAAAATTAAAAGGTATGACTCCCTTAGAATTTAGGGAAGCATACCTTATAAAAAACTAACTTTTTTTAACTGTCCATTTTATCT
GGTGCTTGACA
Recoding section
- Recoding by frameshift
- Frame -1
- Type translational
- Experimentally demonstrated No
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 321 bp | 106 aa | 71 | 391 | + | No |
Description : First part of the transposase
ORF sequence :
MAKKGSKFTKYPPEFKLQVVEDYLSGKSGGMPSIVKKYGLKSDNQVLTWTRKYREDPTLISQDLRGRKSTGRPKTRNLEEMTLEEQNTYLRMENAILKTL
RTLLNP
RTLLNP
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 954 bp | 317 aa | 322 | 1275 | + | No |
Description : Second part of the transposase
ORF sequence :
GTEYLSTNGECNFKDLKDSPQSVRRVHLYQVVEQLKDRYSVSSLCAYFGFPRSSYYLWLSKGRPVYKQFDANLAQKITAIFHDTQKGYRFISYQLRRHYQ
LSPNPKTVLRYMRILGHQSPIRKKRYQSCTQREINEKARHVHYNVLVRNFKAERPLQKLTTDVSYVYHKQGRMFLSVIKDCYDNSILAYTLSDFNDNKLV
FDNIDLVFHDSWDSTKVCILHSDQGFQYTNQQYLRKLDQYGVTISHSGKGNCYDNAACENFFSHLKSESLRLFPPEDREALIQQIREYIDWYNWHRPQEK
LKGMTPLEFREAYLIKN
LSPNPKTVLRYMRILGHQSPIRKKRYQSCTQREINEKARHVHYNVLVRNFKAERPLQKLTTDVSYVYHKQGRMFLSVIKDCYDNSILAYTLSDFNDNKLV
FDNIDLVFHDSWDSTKVCILHSDQGFQYTNQQYLRKLDQYGVTISHSGKGNCYDNAACENFFSHLKSESLRLFPPEDREALIQQIREYIDWYNWHRPQEK
LKGMTPLEFREAYLIKN
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1205 bp | 401 aa | 71 | 1275 | + | Yes |
Chemistry : DDE
ORF sequence :
MAKKGSKFTKYPPEFKLQVVEDYLSGKSGGMPSIVKKYGLKSDNQVLTWTRKYREDPTLISQDLRGRKSTGRPKTRNLEEMTLEEQNTYLRMENAILKDL
KDSPQSVRRVHLYQVVEQLKDRYSVSSLCAYFGFPRSSYYLWLSKGRPVYKQFDANLAQKITAIFHDTQKGYRFISYQLRRHYQLSPNPKTVLRYMRILG
HQSPIRKKRYQSCTQREINEKARHVHYNVLVRNFKAERPLQKLTTDVSYVYHKQGRMFLSVIKDCYDNSILAYTLSDFNDNKLVFDNIDLVFHDSWDSTK
VCILHSDQGFQYTNQQYLRKLDQYGVTISHSGKGNCYDNAACENFFSHLKSESLRLFPPEDREALIQQIREYIDWYNWHRPQEKLKGMTPLEFREAYLIK
N
KDSPQSVRRVHLYQVVEQLKDRYSVSSLCAYFGFPRSSYYLWLSKGRPVYKQFDANLAQKITAIFHDTQKGYRFISYQLRRHYQLSPNPKTVLRYMRILG
HQSPIRKKRYQSCTQREINEKARHVHYNVLVRNFKAERPLQKLTTDVSYVYHKQGRMFLSVIKDCYDNSILAYTLSDFNDNKLVFDNIDLVFHDSWDSTK
VCILHSDQGFQYTNQQYLRKLDQYGVTISHSGKGNCYDNAACENFFSHLKSESLRLFPPEDREALIQQIREYIDWYNWHRPQEKLKGMTPLEFREAYLIK
N
Blast result :
Comments
ISSsu11 is 53% (transposase) aa similar to ISSth1b.
The third ORF is the putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
The third ORF is the putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References