ISBli33

  • Family IS3
  • Group IS3
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
CP014869 ND Brevibacterium linens
Brevibacterium linens
Brevibacterium linens BS258
Brevibacterium epidermidis BS258
DNA section
IS Length : 1276 bp

Ends


IR Length : 26/28

IRL : GGGACTGCTGAAGGTTTGGTTGACTTCACGACTGGATAAATTCCAGGAAG
IRR : GGGACTGCTGAAGGTTTAGTTGACTCCAGGGTTTATGCCGCCAGAGCGAC

Insertion site


Left flankDirect repeatRight flankDR Length
CTGGATATCGGGACGCGATACCG3
GCCCGGGCTGAAGCGACCCGTTT3

DNA sequence

GGGACTGCTGAAGGTTTGGTTGACTTCACGACTGGATAAATTCCAGGAAGGATGGAAGTCATGCCAAAGATCTACACCGATGAGTTCAAGCAATCAGCGC
TCGAGCTCCTGGGCGAGGGGATGACCCAGAAGCAGGTCTGCGCCGACCTCGGCATCTCGAAGTCAGCTCTCCAGGCCTGGGTACGTGACTCGCGGCTACG
CGAGCACGGACTCGAGCCCTCCCGTGATGTCGAGGAGTCTCGCGCCCAGGCGGCAGCGCTGAAACGCATTCGCGAGCTCGAGAGGGAGAACAAGATCCTC
CGCGAGGCTGCAGCGTATCTCTCGCAAGCGAACTTGAGGCTCGGTGGTCATCACCCCAAATGATGTACCCGCTGGTCCTCGACCCTGCTGCCACCGGGGT
GCCGGTGGCAGTGGCCTGCCGGGTCCTCGGCTTTACCAAGCAGGGCTTCTACAAGTGGTGCGCGAAACCTATCTCTGACCGGGATCTCGAGGAGGCGCAC
TTGATCAATGCCGCCTACGACGTGCATACGGACGATCCCGAGTTCGGGCACCGGTTCATCGCTGATGAACTCCACGCGTGCGGATACGAGGCGTCAGAGC
GTCGTGTGTGGCGGCTGTGCTCGCAGCAGGAACTGTTCTCCCTCACGATCAAACGGGCCCGGAAGCACGGTAAGAAGCCCGGCCCGCCAGTGTGTGATGA
CCTCGTCGAACGCGACTTCTCAGCGTCAGGCATCAACGAACTATGGTTGACCGACATTACCGAGCATCACACCAGTGAGGGGAAACTGTACATGTGCGCG
GTCAAGGATGTGTTCAGCAACAGAATCGTCGGTTATGCCATCGATTCGAGAATGAAATCTCGCCTGGCTGTGGCTGCGATTGATGATGCTGTAGCCCGTC
GTGGCGGACAGCGAGCCGTGGCCGGTTGCGTGGTGCATTCGGATCGCGGGTCGCAATTTCGTTCTCGGAAAATCCAGCAGGTTCTACGGCATTACGGGCT
CGTGGGGTCGATGGGAAGGGTTGGTGCTGCTGGTGATAATGCGGCGATGGAATCGTTCTTCGCCGTGTTGCAGAAGAACGTCCTCGACCGGGGCCGTTGG
GACACGCGAGCGAGGTTGCGCACAGCGATCGTGCACTGGGTCGAACGAACCTATCACCGGAAGCGTCGACAGAGGAGACTGGATAAACTGACCCCGGTTG
AATACGAGCTTGTTCACCACAAGGCCGTCGCTCTGGCGGCATAAACCCTGGAGTCAACTAAACCTTCAGCAGTCCC
Recoding section
  • Recoding by frameshift
  • Frame -1
  • Type translational
  • Experimentally demonstrated No

Stimulators :

  • Shine-Dalgarno sequence :
  • Secondary structure :

Recoding motif :

Protein section
ORF number : 3

 

ORF 1
LengthBeginEndStrandFusion ORF
303 bp100 aa61363+No
ORF function : Transposase
Description : First part of the transposase

ORF sequence :

MPKIYTDEFKQSALELLGEGMTQKQVCADLGISKSALQAWVRDSRLREHGLEPSRDVEESRAQAAALKRIRELERENKILREAAAYLSQANLRLGGHHPK

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
1017 bp338 aa2281244+No
ORF function : Transposase
Description : Second part of the transposase

ORF sequence :

CRGVSRPGGSAETHSRAREGEQDPPRGCSVSLASELEARWSSPQMMYPLVLDPAATGVPVAVACRVLGFTKQGFYKWCAKPISDRDLEEAHLINAAYDVH
TDDPEFGHRFIADELHACGYEASERRVWRLCSQQELFSLTIKRARKHGKKPGPPVCDDLVERDFSASGINELWLTDITEHHTSEGKLYMCAVKDVFSNRI
VGYAIDSRMKSRLAVAAIDDAVARRGGQRAVAGCVVHSDRGSQFRSRKIQQVLRHYGLVGSMGRVGAAGDNAAMESFFAVLQKNVLDRGRWDTRARLRTA
IVHWVERTYHRKRRQRRLDKLTPVEYELVHHKAVALAA

 

Blast result :
ORF 3
LengthBeginEndStrandFusion ORF
1184 bp395 aa611244+Yes
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MPKIYTDEFKQSALELLGEGMTQKQVCADLGISKSALQAWVRDSRLREHGLEPSRDVEESRAQAAALKRIRELERENKILREAAAYLSQANLRLGGHHPP
QMMYPLVLDPAATGVPVAVACRVLGFTKQGFYKWCAKPISDRDLEEAHLINAAYDVHTDDPEFGHRFIADELHACGYEASERRVWRLCSQQELFSLTIKR
ARKHGKKPGPPVCDDLVERDFSASGINELWLTDITEHHTSEGKLYMCAVKDVFSNRIVGYAIDSRMKSRLAVAAIDDAVARRGGQRAVAGCVVHSDRGSQ
FRSRKIQQVLRHYGLVGSMGRVGAAGDNAAMESFFAVLQKNVLDRGRWDTRARLRTAIVHWVERTYHRKRRQRRLDKLTPVEYELVHHKAVALAA

 

Blast result :
Comments
ISBli33 is 76% aa similar to ISAar45.
The third ORF is the putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References
1] Sebastien Levesque (2017) Direct submission.
2] Zhu,Y. and Sun,C. (2016) Direct GenBank submission.