ISBt2
- Family IS3
- Group IS150
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| CP001186 | ND | Bacillus thuringiensis | Bacillus thuringiensis subsp. thuringiensis HD770 Bacillus thuringiensis YBT-226 Bacillus thuringiensis subsp. galleriae 916 Bacillus thuringiensis subsp. thuringiensis HD201 Bacillus cereus G9842 |
DNA section
IS Length : 1293 bp
Ends
IR Length : 16/30
IRL : TGACTGAGTATTGAAGTGAACCCGAATAGTGGTACATGAAAAAAACACCT
IRR : TGAAGTGAACCCCGAAAACGGGACACCAATCAAAACACCTAAACCACTTG
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| TATATAAAAAGC | GTATAGTCAG | 0 |
DNA sequence
TGACTGAGTATTGAAGTGAACCCGAATAGTGGTACATGAAAAAAACACCTCCTGAATTCGCATACTAAGTATGCAAATTCATTGGAGGTGTTTTTCGTTT
GAAGACAAGAGTTCATTACCCAGAAGAAGTAAAGTGGAAAGTGATTGAAATGAAAAAGGATGGATATTCCAATTGGACAATTATGGAGAAGCTTGGAATT
AAAAATGTTTCCCAAATTAAGACGTGGATGAAGTGGTATCGTACCGATCAAACGTACCGTTTTCAACAACCTGTAGGAAAACAATATTCTTATGGAAAAG
GACCAAAAGAGCTCAGTGAATTAGAACAGCTACGTTTAGAAAATAAACATCTAAAAACAAAATTACTTGTATGGGGAAAGTATCTGGAAATCGAAAGGGG
CTGAAACCAGAAACTGTAGTTACTTTATGGAGAAATGTACAAACAAAAATAACGGTAAAAGACTTGTGTAACGTATTAGAATTACCCCGTTCTACATTTT
ATCGTTGGTTACAACGAACGGAAGATCTGAAAGATGATATAGAAGAAAAAGTAAAGGACGTCTGTCTTAGGCATAAATTCCGGTATGGATATCGAAGAGT
GACCGCTACTCTTCGAAAAATGGGACTGTGTGTCAATCATAAAAAAGTATTACGAATCATGAGACAAAATCATATTCTTTCCAAAGTGCGTCGAAAGAAA
AAGAAATATATCAATGGTGCGGAACCAATGGTAGCCCCTCATCGATTGGAGCGCCAATTCGATGCATCCAAACCAAATGAAAAGTGGTTTACGGATGTGA
CGTATCTCTTATTTGGAGAGCGTACCTTGTATGTATCAACGATCATGGATGCCTTTAATCGTGAAATTATTAGTTGTGTCATCAGCGAATCACAAACACT
GACATTAGCGATGAAAACATTGAAACAAGCAATGAGAGGGCGAAAAGTAAAAGATGTCCTTCTTCACTCGGATCAAGGAAGTATTTATACAGCAAAAGAA
TTTCAAGCGTATGCCAAAGAAAACGGCATGATCACCAGCATGTCCCGGAGAGGAAATTGCCATGATAATGCCGTCATGGAAAGCTTCTTTGGTCATTTAA
AAAGTGAAGCTTTCTATTCACAAAAGATAACAAAAGTATCCAACACAACTGTGCGAAAGATTGTGCTAGAATACATTCATTACTACAATTGTGTGCGAAT
TCAAGAAAAATTAAACCACCTATCCCCTAAAGAATTTAGGGAACAAGTGGTTTAGGTGTTTTGATTGGTGTCCCGTTTTCGGGGTTCACTTCA
GAAGACAAGAGTTCATTACCCAGAAGAAGTAAAGTGGAAAGTGATTGAAATGAAAAAGGATGGATATTCCAATTGGACAATTATGGAGAAGCTTGGAATT
AAAAATGTTTCCCAAATTAAGACGTGGATGAAGTGGTATCGTACCGATCAAACGTACCGTTTTCAACAACCTGTAGGAAAACAATATTCTTATGGAAAAG
GACCAAAAGAGCTCAGTGAATTAGAACAGCTACGTTTAGAAAATAAACATCTAAAAACAAAATTACTTGTATGGGGAAAGTATCTGGAAATCGAAAGGGG
CTGAAACCAGAAACTGTAGTTACTTTATGGAGAAATGTACAAACAAAAATAACGGTAAAAGACTTGTGTAACGTATTAGAATTACCCCGTTCTACATTTT
ATCGTTGGTTACAACGAACGGAAGATCTGAAAGATGATATAGAAGAAAAAGTAAAGGACGTCTGTCTTAGGCATAAATTCCGGTATGGATATCGAAGAGT
GACCGCTACTCTTCGAAAAATGGGACTGTGTGTCAATCATAAAAAAGTATTACGAATCATGAGACAAAATCATATTCTTTCCAAAGTGCGTCGAAAGAAA
AAGAAATATATCAATGGTGCGGAACCAATGGTAGCCCCTCATCGATTGGAGCGCCAATTCGATGCATCCAAACCAAATGAAAAGTGGTTTACGGATGTGA
CGTATCTCTTATTTGGAGAGCGTACCTTGTATGTATCAACGATCATGGATGCCTTTAATCGTGAAATTATTAGTTGTGTCATCAGCGAATCACAAACACT
GACATTAGCGATGAAAACATTGAAACAAGCAATGAGAGGGCGAAAAGTAAAAGATGTCCTTCTTCACTCGGATCAAGGAAGTATTTATACAGCAAAAGAA
TTTCAAGCGTATGCCAAAGAAAACGGCATGATCACCAGCATGTCCCGGAGAGGAAATTGCCATGATAATGCCGTCATGGAAAGCTTCTTTGGTCATTTAA
AAAGTGAAGCTTTCTATTCACAAAAGATAACAAAAGTATCCAACACAACTGTGCGAAAGATTGTGCTAGAATACATTCATTACTACAATTGTGTGCGAAT
TCAAGAAAAATTAAACCACCTATCCCCTAAAGAATTTAGGGAACAAGTGGTTTAGGTGTTTTGATTGGTGTCCCGTTTTCGGGGTTCACTTCA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 306 bp | 101 aa | 99 | 404 | + | No |
Description : First part of the transposase
ORF sequence :
LKTRVHYPEEVKWKVIEMKKDGYSNWTIMEKLGIKNVSQIKTWMKWYRTDQTYRFQQPVGKQYSYGKGPKELSELEQLRLENKHLKTKLLVWGKYLEIER
G
G
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 909 bp | 302 aa | 347 | 1255 | + | No |
Description : Second part of the transposase
ORF sequence :
TSKNKITCMGKVSGNRKGLKPETVVTLWRNVQTKITVKDLCNVLELPRSTFYRWLQRTEDLKDDIEEKVKDVCLRHKFRYGYRRVTATLRKMGLCVNHKK
VLRIMRQNHILSKVRRKKKKYINGAEPMVAPHRLERQFDASKPNEKWFTDVTYLLFGERTLYVSTIMDAFNREIISCVISESQTLTLAMKTLKQAMRGRK
VKDVLLHSDQGSIYTAKEFQAYAKENGMITSMSRRGNCHDNAVMESFFGHLKSEAFYSQKITKVSNTTVRKIVLEYIHYYNCVRIQEKLNHLSPKEFREQ
VV
VLRIMRQNHILSKVRRKKKKYINGAEPMVAPHRLERQFDASKPNEKWFTDVTYLLFGERTLYVSTIMDAFNREIISCVISESQTLTLAMKTLKQAMRGRK
VKDVLLHSDQGSIYTAKEFQAYAKENGMITSMSRRGNCHDNAVMESFFGHLKSEAFYSQKITKVSNTTVRKIVLEYIHYYNCVRIQEKLNHLSPKEFREQ
VV
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1157 bp | 385 aa | 99 | 1255 | + | Yes |
Chemistry : DDE
ORF sequence :
LKTRVHYPEEVKWKVIEMKKDGYSNWTIMEKLGIKNVSQIKTWMKWYRTDQTYRFQQPVGKQYSYGKGPKELSELEQLRLENKHLKNKITCMGKVSGNRK
GLKPETVVTLWRNVQTKITVKDLCNVLELPRSTFYRWLQRTEDLKDDIEEKVKDVCLRHKFRYGYRRVTATLRKMGLCVNHKKVLRIMRQNHILSKVRRK
KKKYINGAEPMVAPHRLERQFDASKPNEKWFTDVTYLLFGERTLYVSTIMDAFNREIISCVISESQTLTLAMKTLKQAMRGRKVKDVLLHSDQGSIYTAK
EFQAYAKENGMITSMSRRGNCHDNAVMESFFGHLKSEAFYSQKITKVSNTTVRKIVLEYIHYYNCVRIQEKLNHLSPKEFREQVV
GLKPETVVTLWRNVQTKITVKDLCNVLELPRSTFYRWLQRTEDLKDDIEEKVKDVCLRHKFRYGYRRVTATLRKMGLCVNHKKVLRIMRQNHILSKVRRK
KKKYINGAEPMVAPHRLERQFDASKPNEKWFTDVTYLLFGERTLYVSTIMDAFNREIISCVISESQTLTLAMKTLKQAMRGRKVKDVLLHSDQGSIYTAK
EFQAYAKENGMITSMSRRGNCHDNAVMESFFGHLKSEAFYSQKITKVSNTTVRKIVLEYIHYYNCVRIQEKLNHLSPKEFREQVV
Blast result :
Comments
ISBt2 is 66% aa similar to ISHaha4. The third ORF is the putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
August 2 2012 : the file with partial sequence (Accession number : S68409 [P]) was replaced by the most closely related sequence (99% of DNA identity).
ISBt2 is related to IS150. ISBt2 is located downstream of the CryIIB gene on a 6.5 kb HindIII fragment (Hodgman et al., 1993). The 45-aa ORF is 41.8 % identical to the C-terminal end of IS150 ORF2.
August 2 2012 : the file with partial sequence (Accession number : S68409 [P]) was replaced by the most closely related sequence (99% of DNA identity).
ISBt2 is related to IS150. ISBt2 is located downstream of the CryIIB gene on a 6.5 kb HindIII fragment (Hodgman et al., 1993). The 45-aa ORF is 41.8 % identical to the C-terminal end of IS150 ORF2.
References
1] Ahmad, W., Nicholls, C., and Ellar, D.J. (1989) FEMS Microbiol. Lett. 59, 197-202.
2] Hodgman, T.C., Yu, Z., Shen, J., and Ellar, D.J. (1993) FEMS Microbiol. Lett. 114, 23-30.
3] Dodson,R.J., Durkin,A.S., Rosovitz,M.J., Rasko,D.A., Hoffmaster,A., Ravel,J. and Sutton,G. (2008) Direct submission GenBank).
2] Hodgman, T.C., Yu, Z., Shen, J., and Ellar, D.J. (1993) FEMS Microbiol. Lett. 114, 23-30.
3] Dodson,R.J., Durkin,A.S., Rosovitz,M.J., Rasko,D.A., Hoffmaster,A., Ravel,J. and Sutton,G. (2008) Direct submission GenBank).