ISCfr25
- Family IS3
- Group IS3
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| ND | Citrobacter freundii | Citrobacter freundii Citrobacter freundii strain CAV1741 Citrobacter freundii strain CAV1741 plasmid pCAV1741-16 Serratia marcescens strain CAV1492. Klebsiella oxytoca strain CAV1374 |
DNA section
IS Length : 1260 bp
Ends
IR Length : 20/26
IRL : TGTAGTGGCATAGTGAATTTGGCCACCTGATTAAATCGCGTGATAGGATC
IRR : TGTAGTGGTCAAGAAAAATTGGCCACGGTCTTATGCTCTAACCAGAACAA
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| GAATACCTTT | GCC | CTGGTGATCT | 3 |
DNA sequence
TGTAGTGGCATAGTGAATTTGGCCACCTGATTAAATCGCGTGATAGGATCACGCCCTCATAGACAGGTGGAAAAATGACCGAAAAACACAGACCCTCTTT
TAGCCCCGAATTCAAACTTGAAGCGGCCCAACTGGTCACCGAAAAGGGATACAGCACCCGAGAGGCTGCGGAGGTCATGGGCGTCAGCCATAGCGCCATG
CGGACCTGGGTGAAACAGCTTAGGAGTGAGCGCCAAGGCATCAGTCCCAAGGCCTCGCCGCTGACGCCAGAACAACAAAAAATCCGAGAATTAGAGAGAA
AAATCAAACGGATAGAAGAGGAAAAAGAAATTTTAAAAAAGGCTACCGCTCTCTTGATGTCGGACTCCCTGAACAATTCTCGCTAGTCGAGCGACTCAAG
AAGAGCTTTAGCGTCAAACGCATCTGCGATGTATTCGGGATCCATCGCAGCAGTTATAAGTACTGGGCGGCGCGTCCTCAAGCCATCTCTTCGGAGAAGG
TAAAGCTGCAAGCCGAAGTCCGTAAGGCCCACAACGACAGCAAGGGCTCAGCGGGTGCCAGGACGATTGCAGACATTGTCACGTCCCGCGGCCAGTCTTT
AAGCCGCTATCGGGCAAGCAAACTGATGAAGGAGCTGGGGTTGGTCAGTTGCCAGCCACCCAAGCATGCTTACAAAAAGGCGCATCAAGAGCATGTTGCC
ATCCCTAACCTATTGGATAGGCAGTTCGCGGTGACGGCGCCGAATCAAGTCTGGTGTGGTGATGTGACCTACATCTGGACCGGAACGCAATGGGCCTATC
TGGCGGTGGTGCTGGACCTGTTTGCCCGCAAGCCAGTGGGGTGGGCGATGTCGTTGTCCCCGGATAGCGAGTTGACCAGCAAGGCATTGATGGTGGCTTT
TGAGGCCCGAGGCCGACCCAAGGGGCTACTGTTTCATTCTGACCAGGGAAGCCATTACACCAGCCGTCGATTCCGGCAGCAGCTTTGGCGCTACCAGATC
AGACAGAGCATGAGCCGACGCGGTAACTGCTGGGACAATGCTCCGATGGAGCGATTGTTTAGAAGCTTAAAGACCGAGTGGATGCCCACGACCGGTTACC
GCAGTGTTAACGAAGCCAAACAGGCAATCACGGATTATCTGGTGGGCTACTACAGCCAGGTTAGACCGCACTCCTACAATGGTGGTCTGACACCGAATGA
ATCCGAACGATTGTTCTGGTTAGAGCATAAGACCGTGGCCAATTTTTCTTGACCACTACA
TAGCCCCGAATTCAAACTTGAAGCGGCCCAACTGGTCACCGAAAAGGGATACAGCACCCGAGAGGCTGCGGAGGTCATGGGCGTCAGCCATAGCGCCATG
CGGACCTGGGTGAAACAGCTTAGGAGTGAGCGCCAAGGCATCAGTCCCAAGGCCTCGCCGCTGACGCCAGAACAACAAAAAATCCGAGAATTAGAGAGAA
AAATCAAACGGATAGAAGAGGAAAAAGAAATTTTAAAAAAGGCTACCGCTCTCTTGATGTCGGACTCCCTGAACAATTCTCGCTAGTCGAGCGACTCAAG
AAGAGCTTTAGCGTCAAACGCATCTGCGATGTATTCGGGATCCATCGCAGCAGTTATAAGTACTGGGCGGCGCGTCCTCAAGCCATCTCTTCGGAGAAGG
TAAAGCTGCAAGCCGAAGTCCGTAAGGCCCACAACGACAGCAAGGGCTCAGCGGGTGCCAGGACGATTGCAGACATTGTCACGTCCCGCGGCCAGTCTTT
AAGCCGCTATCGGGCAAGCAAACTGATGAAGGAGCTGGGGTTGGTCAGTTGCCAGCCACCCAAGCATGCTTACAAAAAGGCGCATCAAGAGCATGTTGCC
ATCCCTAACCTATTGGATAGGCAGTTCGCGGTGACGGCGCCGAATCAAGTCTGGTGTGGTGATGTGACCTACATCTGGACCGGAACGCAATGGGCCTATC
TGGCGGTGGTGCTGGACCTGTTTGCCCGCAAGCCAGTGGGGTGGGCGATGTCGTTGTCCCCGGATAGCGAGTTGACCAGCAAGGCATTGATGGTGGCTTT
TGAGGCCCGAGGCCGACCCAAGGGGCTACTGTTTCATTCTGACCAGGGAAGCCATTACACCAGCCGTCGATTCCGGCAGCAGCTTTGGCGCTACCAGATC
AGACAGAGCATGAGCCGACGCGGTAACTGCTGGGACAATGCTCCGATGGAGCGATTGTTTAGAAGCTTAAAGACCGAGTGGATGCCCACGACCGGTTACC
GCAGTGTTAACGAAGCCAAACAGGCAATCACGGATTATCTGGTGGGCTACTACAGCCAGGTTAGACCGCACTCCTACAATGGTGGTCTGACACCGAATGA
ATCCGAACGATTGTTCTGGTTAGAGCATAAGACCGTGGCCAATTTTTCTTGACCACTACA
Recoding section
- Recoding by frameshift
- Frame -1
- Type translational
- Experimentally demonstrated No
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 312 bp | 103 aa | 75 | 386 | + | No |
Description : First part of the transposase
ORF sequence :
MTEKHRPSFSPEFKLEAAQLVTEKGYSTREAAEVMGVSHSAMRTWVKQLRSERQGISPKASPLTPEQQKIRELERKIKRIEEEKEILKKATALLMSDSLN
NSR
NSR
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1023 bp | 340 aa | 230 | 1252 | + | No |
Description : Second part of the transposase
ORF sequence :
APRHQSQGLAADARTTKNPRIREKNQTDRRGKRNFKKGYRSLDVGLPEQFSLVERLKKSFSVKRICDVFGIHRSSYKYWAARPQAISSEKVKLQAEVRKA
HNDSKGSAGARTIADIVTSRGQSLSRYRASKLMKELGLVSCQPPKHAYKKAHQEHVAIPNLLDRQFAVTAPNQVWCGDVTYIWTGTQWAYLAVVLDLFAR
KPVGWAMSLSPDSELTSKALMVAFEARGRPKGLLFHSDQGSHYTSRRFRQQLWRYQIRQSMSRRGNCWDNAPMERLFRSLKTEWMPTTGYRSVNEAKQAI
TDYLVGYYSQVRPHSYNGGLTPNESERLFWLEHKTVANFS
HNDSKGSAGARTIADIVTSRGQSLSRYRASKLMKELGLVSCQPPKHAYKKAHQEHVAIPNLLDRQFAVTAPNQVWCGDVTYIWTGTQWAYLAVVLDLFAR
KPVGWAMSLSPDSELTSKALMVAFEARGRPKGLLFHSDQGSHYTSRRFRQQLWRYQIRQSMSRRGNCWDNAPMERLFRSLKTEWMPTTGYRSVNEAKQAI
TDYLVGYYSQVRPHSYNGGLTPNESERLFWLEHKTVANFS
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1178 bp | 392 aa | 75 | 1252 | + | Yes |
Chemistry : DDE
ORF sequence :
MTEKHRPSFSPEFKLEAAQLVTEKGYSTREAAEVMGVSHSAMRTWVKQLRSERQGISPKASPLTPEQQKIRELERKIKRIEEEKEILKKGYRSLDVGLPE
QFSLVERLKKSFSVKRICDVFGIHRSSYKYWAARPQAISSEKVKLQAEVRKAHNDSKGSAGARTIADIVTSRGQSLSRYRASKLMKELGLVSCQPPKHAY
KKAHQEHVAIPNLLDRQFAVTAPNQVWCGDVTYIWTGTQWAYLAVVLDLFARKPVGWAMSLSPDSELTSKALMVAFEARGRPKGLLFHSDQGSHYTSRRF
RQQLWRYQIRQSMSRRGNCWDNAPMERLFRSLKTEWMPTTGYRSVNEAKQAITDYLVGYYSQVRPHSYNGGLTPNESERLFWLEHKTVANFS
QFSLVERLKKSFSVKRICDVFGIHRSSYKYWAARPQAISSEKVKLQAEVRKAHNDSKGSAGARTIADIVTSRGQSLSRYRASKLMKELGLVSCQPPKHAY
KKAHQEHVAIPNLLDRQFAVTAPNQVWCGDVTYIWTGTQWAYLAVVLDLFARKPVGWAMSLSPDSELTSKALMVAFEARGRPKGLLFHSDQGSHYTSRRF
RQQLWRYQIRQSMSRRGNCWDNAPMERLFRSLKTEWMPTTGYRSVNEAKQAITDYLVGYYSQVRPHSYNGGLTPNESERLFWLEHKTVANFS
Blast result :
Comments
ISCfr25 is 87% aa similar to ISSlo2.
The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References
1] Dongsheng ZHOU (2017) Direct submission to ISfinder