ISBli8
- Family IS5
- Group IS427
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| CP017150 | ND | Brevibacterium linens | Brevibacterium linens SMQ-1335 Brevibacterium linens Brevibacterium aurantiacum |
DNA section
IS Length : 971 bp
Ends
IR Length : 18/20
IRL : GGCGTGTCTCCTTTATTGACCATTCCAGGACTGGCACGGTGGAGACGTGA
IRR : GGCATGTCTCCTTTATTGGCGGAGCCATGTCAGGATCGCGCTGATCACGA
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| ACGACTCTGGTT | CTAG | GCGGTCCTCAT | 4 |
DNA sequence
GGCGTGTCTCCTTTATTGACCATTCCAGGACTGGCACGGTGGAGACGTGACTACACAGCAACGACACCGAGTCTTCACTGATGAGCAGTGGGAGAAGATC
GAACCACTTCTGCCCTCAAACGTCGGCAAAAGAGCCCGACCGTTTGAGAACAACCGGCGAATCGTCGAAGGAATCGTCTACCGCTACCGCGCTGGCATCG
CCTGGCGTGACCTGCCGCGCGAGCATTTCGGGCCGTGGCAGACAGTATGGAAACGTCACCGCCGCTACGCCGCCGACGGCACCTGGGACCGAGTCTTAGC
TCGGGTGCTTTCCGATGCTGATGCTGCCGGTGACATCGATTGGAACATCTCCGTCGATGGCACCATCAATCGTGCTCACCAGCACGCGACGAACACGACA
CGCCTCGATCAGGACACAGGGGGCTACGTCGAATTACAAAAATCTGCCTGATTCGGAATGTGAACCCGCCGGGCACGGCATCGGTCGCTCACGTGGTGGG
CTGACGACGAAGATCCATCATGCCGTCGACGGCAAAGGCCGGCCTTTGGCTGTCGTGGTCACCGGCGGACAGCGCCACGACGGGGTGATCCTGCCGCAGG
TGCTGGCTGACATTCGAGTACCGCGGGTCGGCGGTGGTCGTCCTCGCACGTGTCCGGACGCTGTTCTGGCGGATCGAGCGTATGGGTCCAGAGCCAACCG
CGACTATCTGCGTTCGCGTGGTATTCGGGCGGTGATCCCGGAGAAGAAAGACCAGATCGCGACTCGTAAGAAGCGCGGTAGCAAAGGTGGGCGGCCGCCG
GCGTTCGACGCCGGTGTCTACCGGAACCGCAACGTGGTGGAGCGTTCGTTTGCCTACGTCAAGCAATGGCGGGGGTTAGCGACGAGATACGACAAGCTCG
CCATCACCTATCGAGCGGCCGTCGTGATCAGCGCGATCCTGACATGGCTCCGCCAATAAAGGAGACATGCC
GAACCACTTCTGCCCTCAAACGTCGGCAAAAGAGCCCGACCGTTTGAGAACAACCGGCGAATCGTCGAAGGAATCGTCTACCGCTACCGCGCTGGCATCG
CCTGGCGTGACCTGCCGCGCGAGCATTTCGGGCCGTGGCAGACAGTATGGAAACGTCACCGCCGCTACGCCGCCGACGGCACCTGGGACCGAGTCTTAGC
TCGGGTGCTTTCCGATGCTGATGCTGCCGGTGACATCGATTGGAACATCTCCGTCGATGGCACCATCAATCGTGCTCACCAGCACGCGACGAACACGACA
CGCCTCGATCAGGACACAGGGGGCTACGTCGAATTACAAAAATCTGCCTGATTCGGAATGTGAACCCGCCGGGCACGGCATCGGTCGCTCACGTGGTGGG
CTGACGACGAAGATCCATCATGCCGTCGACGGCAAAGGCCGGCCTTTGGCTGTCGTGGTCACCGGCGGACAGCGCCACGACGGGGTGATCCTGCCGCAGG
TGCTGGCTGACATTCGAGTACCGCGGGTCGGCGGTGGTCGTCCTCGCACGTGTCCGGACGCTGTTCTGGCGGATCGAGCGTATGGGTCCAGAGCCAACCG
CGACTATCTGCGTTCGCGTGGTATTCGGGCGGTGATCCCGGAGAAGAAAGACCAGATCGCGACTCGTAAGAAGCGCGGTAGCAAAGGTGGGCGGCCGCCG
GCGTTCGACGCCGGTGTCTACCGGAACCGCAACGTGGTGGAGCGTTCGTTTGCCTACGTCAAGCAATGGCGGGGGTTAGCGACGAGATACGACAAGCTCG
CCATCACCTATCGAGCGGCCGTCGTGATCAGCGCGATCCTGACATGGCTCCGCCAATAAAGGAGACATGCC
Recoding section
- Recoding by frameshift
- Frame +1
- Type
- Experimentally demonstrated No
Stimulators :
- Shine-Dalgarno sequence : No
- Secondary structure : No-structure
Recoding motif :
TCAGGACACAGGGGGCTACGTCGAATTAC
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 405 bp | 134 aa | 47 | 451 | + | No |
Description : First part of the transposase
ORF sequence :
MTTQQRHRVFTDEQWEKIEPLLPSNVGKRARPFENNRRIVEGIVYRYRAGIAWRDLPREHFGPWQTVWKRHRRYAADGTWDRVLARVLSDADAAGDIDWN
ISVDGTINRAHQHATNTTRLDQDTGGYVELQKSA
ISVDGTINRAHQHATNTTRLDQDTGGYVELQKSA
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 626 bp | 219 aa | 334 | 959 | + | No |
Description : Second part of the transposase
ORF sequence :
LGCFPMLMLPVTSIGTSPSMAPSIVLTSTRRTRHASIRTQGATSNYKNLPDSECEPAGHGIGRSRGGLTTKIHHAVDGKGRPLAVVVTGGQRHDGVILPQ
VLADIRVPRVGGGRPRTCPDAVLADRAYGSRANRDYLRSRGIRAVIPEKKDQIATRKKRGSKGGRPPAFDAGVYRNRNVVERSFAYVKQWRGLATRYDKL
AITYRAAVVISAILTWLRQ
VLADIRVPRVGGGRPRTCPDAVLADRAYGSRANRDYLRSRGIRAVIPEKKDQIATRKKRGSKGGRPPAFDAGVYRNRNVVERSFAYVKQWRGLATRYDKL
AITYRAAVVISAILTWLRQ
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 913 bp | 304 aa | 47 | 959 | + | Yes |
Chemistry : DDE
ORF sequence :
MTTQQRHRVFTDEQWEKIEPLLPSNVGKRARPFENNRRIVEGIVYRYRAGIAWRDLPREHFGPWQTVWKRHRRYAADGTWDRVLARVLSDADAAGDIDWN
ISVDGTINRAHQHATNTTRLDQDTGGATSNYKNLPDSECEPAGHGIGRSRGGLTTKIHHAVDGKGRPLAVVVTGGQRHDGVILPQVLADIRVPRVGGGRP
RTCPDAVLADRAYGSRANRDYLRSRGIRAVIPEKKDQIATRKKRGSKGGRPPAFDAGVYRNRNVVERSFAYVKQWRGLATRYDKLAITYRAAVVISAILT
WLRQ
ISVDGTINRAHQHATNTTRLDQDTGGATSNYKNLPDSECEPAGHGIGRSRGGLTTKIHHAVDGKGRPLAVVVTGGQRHDGVILPQVLADIRVPRVGGGRP
RTCPDAVLADRAYGSRANRDYLRSRGIRAVIPEKKDQIATRKKRGSKGGRPPAFDAGVYRNRNVVERSFAYVKQWRGLATRYDKLAITYRAAVVISAILT
WLRQ
Blast result :
Comments
ISBli8 is 60% aa similar to IS1650.
The third ORf is the putative ORFAB transposase reconstructed in silico by possible +1 frameshift.
The third ORf is the putative ORFAB transposase reconstructed in silico by possible +1 frameshift.
References
1] Sebastien Levesque (2017) Direct submission.
2] de Melo,A.G., Labrie,S.J., Dumaresq,J., Roberts,R.J., Tremblay,D.M. and Moineau,S. (2016) Direct GenBank submission.
2] de Melo,A.G., Labrie,S.J., Dumaresq,J., Roberts,R.J., Tremblay,D.M. and Moineau,S. (2016) Direct GenBank submission.