ISfinder Mirror Site

ISNov3

Family IS1595
Group ISNha5

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
NKIS01000002	ND	Novosphingobium sp.	Novosphingobium sp. PASSN1 NODE_24

DNA section

IS Length : 3622 bp

Ends

IR Length : 25

IRL : CGGCATTATGTAGCAAACGCACCAAGCCTGAGGCGAACGTCATCGCCGGT
IRR : CGGCATTATGTAGCAAACGCACCAACGCAATGCTAGATGTAATTGCCTGC

Insertion site

Left flank	Direct repeat	Right flank	DR Length
CACGTGGAAC	ATCACTTG	CCGATGCAGA	8

DNA sequence

CGGCATTATGTAGCAAACGCACCAAGCCTGAGGCGAACGTCATCGCCGGTGATGGCAACGGTGGTTGCTGTGATGATGCCTTGATCGTGAGGCACGCATG
ATTACCGCAATTCTTTTAGCGCTAAGCTGTATCGAAGGAGCTGGTGTGATCACCCCCACCAGTGACCCCATTCATTACTGCCTCAATGCCTCTCCACGCA
TCTGGATCCGTGATAATTTCCAAAGGAAAGCAGTGCACAGTTTTTCGCCTATCCTGCGAAAAAACATCATTATAGAAGATGGAAAAATAAACAAACAATC
TAGTTTCTCTGTGGAACGCTTTTCTCACGGATTCCATCGGGATGCAGCAATATTGAGTTTCGATTTGGGTTTGCGGAAAAAGAGGAGCACCGAACCCCAA
GTCCGCATTTTCTATCATTGGGGGCTTGCCGCTATCCGAGAAAGCCGAGAGTACAGAACATTGAACAGCCGGAGTATTTCCGACATTTCTGAATTGAGCA
GAAACCACCAAGTAGTTCCCAAAAACACCTTCAACTATGCTGTCGGTTGCAAGGCCCTTGTTCAGGCTGTGAAGCGATACGTAAGCTCGGTTTTGCGCCG
AGTGTTGCTCACTGGCGATTTGAATCGCTTGGGTAGCCAAGTCGCCAGCCCGTTTGGCCTCATTCGCGCTCGTTTCAGTATGTCTGGCAGCCTCGCGGGC
CCAGTGAGCCGCGCGCCAAGCAGCCGCGAGGGTGAAGCTCCCTATAGCCAACCCAATAATGCCAGCCCAAAACGTCCGAAAAGCCCACAATGCAGAATCA
GCAGCGGCATCTGCGGCCTTCCATTGGGCGCAAAGATCGCTTTGACGTTCATCCTGTCCGGGCTGGCAACCACCATCTGGTCGCGCAGCTTTTGGGAGTT
CTTCGACCGCCGCAGCAATCCGCTCAAGCTGGCTGGTGGCGTTCTGTTGGGATGGGGTCTGATCGCCTTGAGTTTCTTTGCATTCGCATGGACTGGATAG
GATCAGTCCAAGTGCAAGGATGAGCAGCCGATAGCTTGAAGACATTGGACCAAGAACACCGCTGTGAATTTGCCACGGCTGATCTTGTTCCTGATATTCG
GCTCAGAGTCCATCACGCCAACGTCTGCCAGCTTACCGACAAGCTGGGCGTAGGTAATCCCCTTGCGGGCAAGCTCAGCCTTGAGCAGCCGCTTCACCTT
GTCTTCCCATTCCTTTTCCTGAACCGGCATGTGCGCTCCTATGATGGTCAATGTCACCATATAGAGTGCATTTGCCATTGACAAGCGTGCATTACGTCAT
CATATAAAGTGCATAAGCACTGGAAACGATGACATGCAACACTTTCTCCTCTCTTCCGCAGCCCGCACACTGAGCCTCAAGGCCGTGTTCCGCATGGGCG
AGGACAAGGCGTATCGGACCTTCTGCGAAATGCGCTGGCCTGAGACGGACGGCGAAGCCGTGTGCCCGCGCTGCGGCTGCACTGAGACGTACAACATCAC
CTCGCGCCGCAAGTTCAAGTGCGTCGCCTGCTATCACCAGTTCAGCGTGACCAGCGGCACGATTTTTGCCTCGCGCAAAATGAGCTTCACCGATCTGCTC
GCCGCCATCGTGATCTTTGTGAACGGCGCGAAGGGCGTTGCCGCGCTTCACCTAAGCCGCGATTTGGATTGCCAGTATAAGACGGCCTTTGTCCTTACGC
ACAAGCTGCGCGAGGCGATGGCGCGCGAGGATGCCACGCAGACCCTGCAGGGCGAGATCGAAGTCGATGGCGCTTACTTTGGCGGCTACGTGAAACCTGC
CAACGAGAAGGAAAACCGCCGCGACCGTCGCAAGGTCGTCAACCAGAATGGCAAGCGCCAGGTTGTCGTGGTTGGCCGCGAGCGCGATGGGGAATCATTC
ACCGTGGTTGCCTCGACGGAAGCCAAAGGTGGCGAAGTGGTCGCCGCTCGCATCCATCATATGTCGACTGTCCACGCCGACGAAGCTTCGCACTGGGATG
GGTTGCATGCTAAGTTTGATACGCGCCGCATCAACCACACGGTCGAGTATTCGAATGGTCATGCTTGCACCAATCAGGCCGAATCGTTCTTCTCACGCCT
TCGCCGTATGGAAATCGGTACACACCACCACATAGCAGGGCCGTACCTCGCCAACTACGCTGCTGAGGCTTCTTGGCGCGAGGACAATCGCCGCATTGCC
AACGGCGCTCAAACTGCGATGGTTGGTGTTGCTGCTCTGGATGCGCCTGTCAGCCGCCAGTGGAAGGGGTACTGGCAGCGGTAGTTAGCCGAGGTTCAAT
ATAAACTGACCCTCAGCCCTAAATTCAAAATCAAAATTCTGTAGTACGTCCATGCCGAGAATCACATCAAACGAGTGATTGTCTGCAATATTGATTGCCT
CGACAGGGAACTCAACTCCAAAAAAGGAACGAATTGGTTCGCCGCCATCCAGCCTATCCGTCTCAAAAATAAAACCGACATTTATCCAATAAAGTAGGTG
CGCCTGAACACTATGAACATTCTGGATTGGTCGTTTTGCATGGCTGGCTAGACCCTCACGGGCTATTGTCGCGTGGGAGAGGCAAGTGCGTTGAGCACCT
GTGTCAATAAGTGCGCGATATGCGTTTACGGGGAATGGATTGGCGCTGTTCTGCGATGGCACTGAGACCGGAGTCGGAACAAACTTCTGCAATCCGACAG
GGACGATTGCCTGCCTATTCTCAAGCCTGCAACGCACTGCCCGCATAGGAATAGAACCCCAGGTTATCGACCTGCTCGGTAACTTCCTGCACGGAAAATC
GTTGCTCTCCGAATTGGGTCGTACCGGCGACAAACGCAGCGATAGAATCGTCGTGCAATTCAATCAACCGCTCATCGTGCAGCAGTGCATACCTACCGGG
CGCACGCTCAAGTAGGTCAGGCAGCATATGCTGGAATGCCTCGAAATTCCGATCAACCTCTGTGTCGATAGAATGCTGCATAAAAGCTCTCCCCGCTCTA
CAGAAAAAACTCTTATCACACGCTTCCGATTCGTGTCACGCTGCGATCTTGACAAAACTGAGAGAATGAGTCCCCGGATTCTGTTTGGAATCCGATCGAC
TCGATTCTTGAAATATTATTTCAACGGCGCGCAAGCGACCATTTAGCCGGGTACTGACTGGCATCCAGAATCACCGCGCCATCCCTTCTCCGGCTGAAGG
TGCTGTGAATGGTATTGGCGAGCATCCCACGAGATTCTGGTGTGTCTTCCCTTCCCTGCTTGGCCAGTACGCGAATGGCAATTTCCATTGATGTGAGCGG
CTCATTGACCTCGCGCAGAATAGTGAGCGCTTCACGGCTGCCTGAGCCACGAGGTGTGCCAGCGGGATTCTTCGAAAAGCTGCGTTTGGTCGCAATCTTT
GAAATGTCGTAGCTTGGGCTGAACATTTTGATGACGGCATCAATATGCGCCAACTCGCACTCAAGACGCATGATCTGAAACCGCCGGGCTTGAATTTCCC
CGTCGATGCGAGCGCGCTTGTCAGTCAAAGCGCTGATTGCGTATGTGTCTGCCATATGCCTACATACGCATCGCAGGCAATTACATCTAGCATTGCGTTG
GTGCGTTTGCTACATAATGCCG

Protein section

ORF number : 4

ORF 1

Length		Begin	End	Strand	Fusion ORF
228 bp	75 aa	1230	1003	-	No

ORF function : Passenger Gene
Annotation : Hypothetical proteinDescription :

ORF sequence :

MPVQEKEWEDKVKRLLKAELARKGITYAQLVGKLADVGVMDSEPNIRNKISRGKFTAVFLVQCLQAIGCSSLHLD

Blast result :

ORF 2

Length		Begin	End	Strand	Fusion ORF
951 bp	316 aa	1334	2284	+	No

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MQHFLLSSAARTLSLKAVFRMGEDKAYRTFCEMRWPETDGEAVCPRCGCTETYNITSRRKFKCVACYHQFSVTSGTIFASRKMSFTDLLAAIVIFVNGAK
GVAALHLSRDLDCQYKTAFVLTHKLREAMAREDATQTLQGEIEVDGAYFGGYVKPANEKENRRDRRKVVNQNGKRQVVVVGRERDGESFTVVASTEAKGG
EVVAARIHHMSTVHADEASHWDGLHAKFDTRRINHTVEYSNGHACTNQAESFFSRLRRMEIGTHHHIAGPYLANYAAEASWREDNRRIANGAQTAMVGVA
ALDAPVSRQWKGYWQR

Blast result :

ORF 3

Length		Begin	End	Strand	Fusion ORF
261 bp	86 aa	2981	2721	-	No

ORF function : Passenger Gene
Annotation : Hypothetical proteinDescription :

ORF sequence :

MQHSIDTEVDRNFEAFQHMLPDLLERAPGRYALLHDERLIELHDDSIAAFVAGTTQFGEQRFSVQEVTEQVDNLGFYSYAGSALQA

Blast result :

ORF 4

Length		Begin	End	Strand	Fusion ORF
435 bp	144 aa	3555	3121	-	No

ORF function : Passenger Gene
Annotation : Hypothetical proteinDescription :

ORF sequence :

MADTYAISALTDKRARIDGEIQARRFQIMRLECELAHIDAVIKMFSPSYDISKIATKRSFSKNPAGTPRGSGSREALTILREVNEPLTSMEIAIRVLAKQ
GREDTPESRGMLANTIHSTFSRRRDGAVILDASQYPAKWSLARR

Blast result :

Comments

ISNov3 is 76% aa similar to ISBos1.

References

1] ISfinder annotation (2017)
2] Kojadinovic,M., Villain,A., Puppo,C., Fon Sing,S., Prioretti,L., Hubert,P., Gregori,G., Zhang,Y., Sassi,J.-F., Claverie,J.-M., Blanc,G. and Gontero,B. (2017) Direct GenBank submission.