ISfinder Mirror Site

ISTico1

Family IS1595
Group ISNha5

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
FWZX01000001	ND	Tistlia consotensis	Tistlia consotensis USBA 355

DNA section

IS Length : 4119 bp

Ends

IR Length : 21/23

IRL : CGGCATTATGCAGCAAGCACACCAAGGCTGCTGCACTGCCTCGCCGAGCC
IRR : CGGCATTATGCAGCAAACGCACCTTCCGCCCGATAGACCCTAAGTGGTTG

Insertion site

Left flank	Direct repeat	Right flank	DR Length
CGGCGGGGGTTTT	GCTTTTGG	GCAGCGCCATATC	8

DNA sequence

CGGCATTATGCAGCAAGCACACCAAGGCTGCTGCACTGCCTCGCCGAGCCTCTGGCCCAACCCCGCAAGCTAAGGAGGGAACGATGAGCTAGGGGCCGGG
GCGATGTTCGCCAAGGCGGCTGGGGCCGCGCTTGCGTTAATTGGACTTGTTGCCCTTTTCGCAGGGAACCAATTCGTTCTCGCCAGTCCGATCAATGTGG
AACGGGAGCCAATAACGGAAACGTGTAGTTCTCCGCCCTTTGAAAGCATCTTGATATTTGATTTCGCCAAAAACATACAGGTTATATGTTTTACTATCTG
CGAATCCCAGGAACATAGAGAGCAATTGACTCTTTATAGGAATTGTATGATAGATCTCTTGATTAAGAAAGATATCAATAACATTTTTAACACGCTCCCT
ATCAACCTCGAAAGTCTCCGATCCTACTTTGAAGACCGTGCTAGTTGCAAAATATTTTACTTTGTATGCCGGCGTAGATCCGTAGTTTTTAATGGATACA
ATAATATTAGCAGATTTCCCTCCATCATGGAGGGCCAAATTTACTTTCGAAAAGCCAACATAGGCGCGGATCTCTTTCTGGGTGTTCTTCCGCGCCTCTT
TAATGGAAACAACTGCAGCATTGGCAGTTCTTTTTGTCTCGTGCAATGTGTCTCGCACAAACAATACACCGACGAAGGTAAGAACTATCGTTATAAGCCC
GACGCTTCCCGCAACGATGACGGCGCCATACGTCCAATCAGCCATCGATTTCTGGGCATACAGATCCTCTTCGGCGCGCTGGGCCTCGCGATTGGCCTCT
ACCTTCAGTTTAAGGCAATCGAGAGCTTTCTGAGGTTCGCTGAGCCGCAGGCAGTCGCGTTGAAGTTCATACTGAGTACGCTGTTGTGTATTGGCAGCGG
CATAATCTTCGTACGCGCGGTTGTATTGCTGACTGGCCCCTGACCACCAAATGAACGGTGCTATGAAAGCAAGATAGACGACGAGAATCAGCCAATGCGC
ATTTCGCGAGTACCAATCGCTTCTAGGCATTGGAGCAGAAAGGCAGCGGTGAATTTTCCACGGCTGACCTTGTTCCTCAGGTTGCGTTCATCCTCGTGAA
CGCCAATTGATTCCAGCTTCTGCTGCAATTGGGAATAGGTAATCCCCTGACGCGCCATCTCGCTGCGGATGAGGCCGCGCGCCTTGGCTTCCCAATCGGT
CCTTTCTGGCATGGTCGCTGCTCCTATGATGCGATTTGTCATCATATAGCGTGCTTCTGCCCTTGAAAAGGTGATAGATCGTCATCATCTCTGATGCGTA
GGCACTGGAGACGATGACAATGACCCAACACTTCCTTCTCTCAGCGAAAGCCCGGACCCTCAGCGTGGTCCAGGTGGCTCGCATGTCCGAGGAAGAGGCT
CGGGCCGCATTCCGGGCGATCCGCTGGGCCACGACCGATGGCGACCCCGTCTGCCCGCGCTGCGGCTGCTGTGCTGTCTACGAGTACGCCAGCCGCCCGA
TCTTCAAGTGCAAGGCTTGCGGCCATCAGTTCAGCGTCACCAGCGGGACGATCTTCGCCAGCCGCAAGCTGCCGGTGCGCGACTACCTCCTGGCCATCGC
GATCTTCGTGAACGCCGCCAAGGGCATGTCCGCGCTGCAGCTCGGCCGCGACCTCGATGTGCAGTACCGCACGGCCTACGTCCTGGCGCACAAGCTGCGC
GAGGCGATGGCTGCCGAGAACGAGACCATGACCCTGAGCGGCACGGTCGAGGTTGATGGCGCCTACTTCGGCGGCTACGTCCGGCCGGAGAACCGCAAGG
AGGATCGCGTCGATCTCCGCCTCAAGGCCAACCAATCCGGCAAGCGCCGCGTCGTGGTCGTCATGCGCCAGCGTGGCGGCCGGACGCTGCCGTTCGTCTT
CCGCAGCGAGGGCGAGTCCCTGCCGTCGATCCTGGCCCGCGTCGAGCCGGGCAGCACGATCCACGCGGACGAAGCCCCGAGCTGGGATGAGCTGCACACC
CGGTACGAGACCCGCCGCATCAACCACAGCATTGCCTTCTCGGATGACGGCGCCTGCACCAATCAGGCGGAAAGCTACTTCTCCCGTCTGCGCCGCGCCG
AGTGGGGCCAGCACCACCACATCAGCGGCCGGTATCTCGGCTTCTATGCTGGCGGGGCCGCGTGGCGCGAGGACACGCGCCGGAAGCCCAATGGGGCGCT
CTACGGGCTGCTGATTGCCGCTGCCGCCGCCCATCCGGTTTCGCGGCAGTGGAAGGGGTACTGGCAACGGGCCCGCTAGTCTCGGGCGTACAGACACTTC
AATGCGAGGAATATGGCCATTTCCTCGCGTCTGGGAGATAGCAAGTAAACCATCAAGTCATGGAATAATTCAGGGGTCATTCTAAAGACAAAAATGTCTT
TTCTGTCGTCTCTTACACTCTCAAACCAGTTTATAACGTGTATTGTGAAGTGAAATGCGAGGTAAGTTCCGCGTCCTGCATTTAGGTCTAGCCTTGCTTC
TGCTTTCTCAGAATCGCTTCCATTGAAAATTATGCCATCAGCCGATGGGTCATACTCATAAATATCATTTACCCAATCCACTGTTGCTAGCTGGTATAGC
AATATAGCCATCATCTTTAAGTGGGAAAGTCCAGATGAATCAGACATGACCCACTCGCTTTCCCACAACTCGCAGCGGCTCCGCCAATTATCGTGGGCCA
CCTTCAGTGCTGATTCATCAAAATCAAAGTAATAACCAACAGCCTTTGCCATGATCTGGCAATGGCGGAGGAAATAATACTTAAATTCGGAAAAATTGCC
CAGCCACTCGTCTAGGCAATCCCGAGTAAGATGCTGATTGTGTGGAGATGCTTCGTTGGACATAAACGCCACTTACCCCATTTTAGGGTGTAGCGTCTAG
TCCAACAAGCAATGGTGGAAACGCGCTTTAGCTCGTCGCGGCCACTTTCTGCCGCTTACGTTCCCCAATCAAAACGCGAATCGTGCGCGTCTCCAGGGGA
GCGCTATAGCCCTCCGGAATGCTCGGACGGCCATTTGTGAAGAGGAACTTAAACCGCTCCTCATCGCGCTTAACTGGCTCGCCGCGACCTACGACGCCCT
GATGCTTCGCCATTGCACCACCCCTCCTCGCGCGCTCCGTGGAGTGTCCCGAAACGCTGCTTATTCGGGCTATATGCAGCATGCGGCCCGCCAGGTCAAA
CAAGGAGTGAGGGCTAAACCTCTTTTCCACTTGACAGCTCCGACGCGCCGGCGGGTGTTTCGCACACCGATTCGACGCTACTAAGACGTGCGTGTGACCA
CGAAAGTACGCCCAGGTCAACGGGCATTAAGTTAAGCCGTTCCCGATGTTTTTTCAAAAGACCTGTGACAATATGGCACGTGTACTACTCGATACAGCGC
CGGGAAGCCCGCAGCGCCTGCTTTCGCTTCTCGACCCTTCCGGCCGTCACGAGCTGGTCTGCCCGCTTGGCTTCGTCCGGATCGATCAGGCAATGCCAGT
AGCCGTCCGCCAGCTTGGCGAGGACGCGGGCCTCTAGGTCTTCCTCGGTGTCTGCCATGCGCTCCCTCTCGCATGAGAACGTAGAGGGGACAAGCGTGAT
TCACGCAAGGCCGCGTCGCACTGCCTACCGCTTCGGCTTCCACAGCACGACGCCATCCTCCGTCCAATACCGCCGGACGGTACCGCGCAGCTCCTGCCGC
TCCAGCATCTTGAGGACCTTGCGCCGTACCTCGATGTAGGCCGGTGAGGCGGGGTCGAGCGCCTTGCGCTCCAAGATGGCGTCGCGGAGATCGACGACGG
ACAGCGGGCCGCGCTCGCGCAGAAGATCGAAAATCAGGCGCACCAGCTCGCCGCGCTTGAAGTAGCCGCCGGGGTACGGGCGGATCGCCTTCACCGGCTT
GCCGTCCCGGTGCCCGAAGATGCCGAGCGTGGCCTCTACGTGGGCGACCTGGCGCTCAAGGACGTCCAGCTTCTCCCGCGTGACTTCCAGCTCGCCAAGC
AGCCTGGCGCGCTTGTCCATGAGCGCGGAGATGACGTGTGGCTCTCCCATGGCCCGATGGTAAAATTTTCAACCACTTAGGGTCTATCGGGCGGAAGGTG
CGTTTGCTGCATAATGCCG

Protein section

ORF number : 6

ORF 1

Length		Begin	End	Strand	Fusion ORF
840 bp	279 aa	104	943	+	No

ORF function : Passenger Gene
Annotation : Hypothetical proteinDescription :

ORF sequence :

MFAKAAGAALALIGLVALFAGNQFVLASPINVEREPITETCSSPPFESILIFDFAKNIQVICFTICESQEHREQLTLYRNCMIDLLIKKDINNIFNTLPI
NLESLRSYFEDRASCKIFYFVCRRRSVVFNGYNNISRFPSIMEGQIYFRKANIGADLFLGVLPRLFNGNNCSIGSSFCLVQCVSHKQYTDEGKNYRYKPD
ASRNDDGAIRPISHRFLGIQILFGALGLAIGLYLQFKAIESFLRFAEPQAVALKFILSTLLCIGSGIIFVRAVVLLTGP

Blast result :

ORF 2

Length		Begin	End	Strand	Fusion ORF
225 bp	74 aa	1212	988	-	No

ORF function : Passenger Gene
Annotation : Hypothetical proteinDescription :

ORF sequence :

MPERTDWEAKARGLIRSEMARQGITYSQLQQKLESIGVHEDERNLRNKVSRGKFTAAFLLQCLEAIGTREMRIG

Blast result :

ORF 3

Length		Begin	End	Strand	Fusion ORF
960 bp	319 aa	1320	2279	+	No

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MTQHFLLSAKARTLSVVQVARMSEEEARAAFRAIRWATTDGDPVCPRCGCCAVYEYASRPIFKCKACGHQFSVTSGTIFASRKLPVRDYLLAIAIFVNAA
KGMSALQLGRDLDVQYRTAYVLAHKLREAMAAENETMTLSGTVEVDGAYFGGYVRPENRKEDRVDLRLKANQSGKRRVVVVMRQRGGRTLPFVFRSEGES
LPSILARVEPGSTIHADEAPSWDELHTRYETRRINHSIAFSDDGACTNQAESYFSRLRRAEWGQHHHISGRYLGFYAGGAAWREDTRRKPNGALYGLLIA
AAAAHPVSRQWKGYWQRAR

Blast result :

ORF 4

Length		Begin	End	Strand	Fusion ORF
186 bp	61 aa	3113	2928	-	No

ORF function : Passenger Gene
Annotation : Hypothetical proteinDescription :

ORF sequence :

MAKHQGVVGRGEPVKRDEERFKFLFTNGRPSIPEGYSAPLETRTIRVLIGERKRQKVAATS

Blast result :

ORF 5

Length		Begin	End	Strand	Fusion ORF
174 bp	57 aa	3558	3385	-	No

ORF function : Passenger Gene
Annotation : Hypothetical proteinDescription :

ORF sequence :

MADTEEDLEARVLAKLADGYWHCLIDPDEAKRADQLVTAGRVEKRKQALRASRRCIE

Blast result :

ORF 6

Length		Begin	End	Strand	Fusion ORF
426 bp	141 aa	4050	3625	-	No

ORF function : Passenger Gene
Annotation : Hypothetical proteinDescription :

ORF sequence :

MGEPHVISALMDKRARLLGELEVTREKLDVLERQVAHVEATLGIFGHRDGKPVKAIRPYPGGYFKRGELVRLIFDLLRERGPLSVVDLRDAILERKALDP
ASPAYIEVRRKVLKMLERQELRGTVRRYWTEDGVVLWKPKR

Blast result :

Comments

ISTico1 is 86% aa similar to ISBmo1.

References

1] ISfinder annotation (2017)
2] Varghese,N. and Submissions,S. (2017) Direct GenBank submission.