ISPmi2

  • Family IS3
  • Group IS3
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
ND Proteus mirabilis
Proteus mirabilis
Proteus mirabilis Pm2CHAMA
DNA section
IS Length : 1592 bp

Ends


IR Length : 21/35

IRL : CCCTGTGTGTCAACATTAGCCTGAACCACTTGTTTTGCAGATAACATACA
IRR : CGGAACGTGTCAACCTCATTTGAACCACTTTCTTAAAAATTAAATTGTTT

Insertion site


Left flankDirect repeatRight flankDR Length
ATCAACCCATCGACCTTTACCATC4

DNA sequence

CCCTGTGTGTCAACATTAGCCTGAACCACTTGTTTTGCAGATAACATACAAATCAGGGTCGTCAGGAGAGTGAACATGCCAAAGCCAGCCACCGTCATTG
AGTCCAAGGTTGAACCCAACCCCGCGCTGGAAAAGCGTACCCGCCGTGTCTTTACGGCAGACTACAAGTTGTCCATCCTGCAACAGGCTGACGCCTGTCA
GCACGGTGAATTGGGTGACCTGCTGCGCCGGGAAAAGCTCTATTCAAACCAGCTCGCGCAGTGGCGTCGTGAGTTTGCCGATCAAGGGGTGGCGGGTCTG
GCCAAGTCCGCGCCCGGCCCCGCCTCCACGAAAAATCCCGATCATCAGCGTATTGCCCAACTGGAAAAAGAAAACGAGCGACTGCGCAAGCAGATTGCCA
TGAAAGATGGTTGCCTGATGCTCCAAAAAAAAGCCTTGGCAATGCTCGAGAGCTTCGAACAGAGCGCCTCATGAACCTGCTCATTGACGATCAGCTGCCC
GCGCACGTGTCGGAACGTCAGGCCTGTGACGCGTTGAACCTGTGCCGTAACAGTCTGCGTGCCGCCCGCGCCCGTGCCCGGTTTTGTGGCCCGGTCAGCC
CGCATCGCCACCGGCGCCGCCCGGCGCCACAACCGCGCGCGTTAAGCGAGGCGGAACGTACGCAGGTGGTGCAGGTTTTGACCAGCGATCACTACCAGGA
TCAACCCCCGGCGCAGGTGTATTACGACCTCTTGCAACAGGGCACGTACCTGTGTTCCGTGTCCACCATGCATCGGCTGATGCGTGCAGAGGGGCTTAAC
GGCGAACGTCGGCCACAGCGACCACGCAGTGCGCAACCGATACCGCGTTTGCTGGCAACCGCGCCCAATCAGGTCTGGACCTGGGACATTGCCAAACTGC
CGACCCAACGCCGTGGCGAGTATCTGTCACTGTATGTTGTGATGGACTTGTTCAGTCGTCATATTGTGGCCTGGATGCTGTCGCGCAAGGAGAACAGCGC
CCTGTCGAGCCAACTGATTGAGCAGGCGTACGAACGCTACGGTATTGCACCGAACAGCCTGACGTTGCACCAGGACCGCGGTGTGCCTATGACGGCGCAT
TGCTATCTGGATCTGTTGGGTGAGCTGGCCATTACGGCCAGTCATAGCCGTCCACGAGTGAGCAATGACAATGCCATGAGCGAGTCCCAGTTCAAAACCA
TGAAATATCAGCCCGACTACCCGCGCCGCTTTGACAACTACGGCCATGCCCTGCGCTGGTGCGATGACTATACGCAGTGGTACAGCCATCAACATCACCA
TAGCGCGCTGGCCGGGTTTACGCCGTATCAGGTGTTCAGTGGTGAATACCTTGAAATCGCTCAGGTCAGGCAGGCTGCGCTGGATGCCATGTATGAACGC
CATCCTGAACGCTTTACCCAGGGCCGACCCACGGTGCAGATGCCGCCGATTAAAGTGTGTATCAACCCGGTACCGGAGGATGCCGACCCAGCCACCCTTG
AAAAAGGCGTCAACTTCCCGACCCTGCCACGGGTCATGGCAAAAACAATTTAATTTTTAAGAAAGTGGTTCAAATGAGGTTGACACGTTCCG
Recoding section
  • Recoding by frameshift
  • Frame -1
  • Type translational
  • Experimentally demonstrated No

Stimulators :

  • Shine-Dalgarno sequence :
  • Secondary structure :

Recoding motif :

Protein section
ORF number : 3

 

ORF 1
LengthBeginEndStrandFusion ORF
399 bp132 aa76474+No
ORF function : Transposase
Description : First part of the transposase

ORF sequence :

MPKPATVIESKVEPNPALEKRTRRVFTADYKLSILQQADACQHGELGDLLRREKLYSNQLAQWRREFADQGVAGLAKSAPGPASTKNPDHQRIAQLEKEN
ERLRKQIAMKDGCLMLQKKALAMLESFEQSAS

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
1281 bp426 aa2731553+No
ORF function : Transposase
Description : Second part of the transposase

ORF sequence :

VCRSRGGGSGQVRARPRLHEKSRSSAYCPTGKRKRATAQADCHERWLPDAPKKSLGNARELRTERLMNLLIDDQLPAHVSERQACDALNLCRNSLRAARA
RARFCGPVSPHRHRRRPAPQPRALSEAERTQVVQVLTSDHYQDQPPAQVYYDLLQQGTYLCSVSTMHRLMRAEGLNGERRPQRPRSAQPIPRLLATAPNQ
VWTWDIAKLPTQRRGEYLSLYVVMDLFSRHIVAWMLSRKENSALSSQLIEQAYERYGIAPNSLTLHQDRGVPMTAHCYLDLLGELAITASHSRPRVSNDN
AMSESQFKTMKYQPDYPRRFDNYGHALRWCDDYTQWYSHQHHHSALAGFTPYQVFSGEYLEIAQVRQAALDAMYERHPERFTQGRPTVQMPPIKVCINPV
PEDADPATLEKGVNFPTLPRVMAKTI

 

Blast result :
ORF 3
LengthBeginEndStrandFusion ORF
1478 bp492 aa761553+Yes
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MPKPATVIESKVEPNPALEKRTRRVFTADYKLSILQQADACQHGELGDLLRREKLYSNQLAQWRREFADQGVAGLAKSAPGPASTKNPDHQRIAQLEKEN
ERLRKQIAMKDGCLMLQKKSLGNARELRTERLMNLLIDDQLPAHVSERQACDALNLCRNSLRAARARARFCGPVSPHRHRRRPAPQPRALSEAERTQVVQ
VLTSDHYQDQPPAQVYYDLLQQGTYLCSVSTMHRLMRAEGLNGERRPQRPRSAQPIPRLLATAPNQVWTWDIAKLPTQRRGEYLSLYVVMDLFSRHIVAW
MLSRKENSALSSQLIEQAYERYGIAPNSLTLHQDRGVPMTAHCYLDLLGELAITASHSRPRVSNDNAMSESQFKTMKYQPDYPRRFDNYGHALRWCDDYT
QWYSHQHHHSALAGFTPYQVFSGEYLEIAQVRQAALDAMYERHPERFTQGRPTVQMPPIKVCINPVPEDADPATLEKGVNFPTLPRVMAKTI

 

Blast result :
Comments
ISPmi2 is 55% aa similar to ISAnsp11 (41% aa identity to ISAnsp11).
The third ORF is the putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
The ISPmi2 ends are not classic for this ISs family (IS3).
This IS has unperfect IRs and begins with CC instead of TG.
References
1] Siebor E. (2017) Direct submission to ISfinder