ISBst1
- Family IS481
- Group
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| AF098273 | ND | Bacillus stearothermophilus | Bacillus stearothermophilus T-6 |
DNA section
IS Length : 1461 bp
Ends
IR Length : 7
IRL : TGTTTCTCCAACGTCCGGATCCTGATTTTACATACATATCCTAAATTGGA
IRR : TGTTTCTTTCGCAAACGGCTCCCGCGAAAGGGAGTAAAACGATTTATACA
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|
DNA sequence
TGTTTCTCCAACGTCCGGATCCTGATTTTACATACATATCCTAAATTGGAATGGTTGAGGTTCCCACAAACCTTTCCTATCGACGGAGAAAAAGCGATCC
GGTACGATAAGGGCAGACGGACCGGAACGGTCCTGATTCCAAGTGAGACAAGGAGGAGATTCGAATGGATGAGTCGATGAGACACGACATTGCCCTGTTT
CGGTACGGGCTAATCGCTCCGTTGGTGAATGGGCAGGTGGAACCGAAGGCGTATCTGAAAGAGGTGAGCGAGCGAGTGCACCATGTTCCCCACCAAGGCG
ACAAACGAATCGCAGCCAAGACGATCCTCGATTGGTGCACCCGATACAAAAAAGGGGGCTTTGACGCCTTAAAGCCGAAGCGCCGTTCGGACCGCGGCCA
CTCAAGGCGTCTGTCGCCCGATGATGAGGATCATATTTTAGCCCTAAGAAAAGAACATCCCACCATGCCCGTGACGGTGTTCTACGAACACCTGATCGAG
CAGGGGGAAATCCCAAAAAACCATATCTCTTACTTTACTATATACCGATTGTTGAAAAAACACAACCTTGTCGGGAAAGAAATCTTGCCCATGCCGGAGC
GAAAGCGCTTTGCGTATGACCAGATCAATGAGCTTTGGCAAGGGGACTTATCCCATGGACCAACGATTCGAGTCAATGGGAAAGCCCAGAAAACGTTTTT
GATCGCCTATATCGATGACTGCTCGCGGTTTGTGCCCGTAACGTATAGTTTCCCTTCGGAGAAGTTTGACGGGCTGCGGATCGTCACGAAGGAAGCGGTG
CTTCGTTGCGGGAAGCCGAAGCGCATTTACTCGGACAACGGGAAAATTTATCGATCCGAGGTGCTGCAGTATGCGTGCGCCGAGATGGGAATTACGCTCA
TCCACACCCAGCCGTATGACCCGCAAAGCAAAGGGAAAATCGAACGGTTCTTCCGCACCGTACAGACGCGGTTTTATCCGCTGATGGAGTTGGATCCCCC
GAAGTCGCTCGAATACCTGAACGAGCGTTTTTGGAGGTGGCTTGAAGAGGAGTATCATCGAAAACCGCACGCCTCGCTGGACGGAAAAACGCCGCATGAG
GTGTTTCAATCGCAAGTGCATCTCGTGTCGTTCATCGAAGATGGCGATTGGCTGGATGCGATTTTTCTGAAGCGGGAGCAACGCAAGGTCAAAGCCGATG
GAACGATCACCTTGAACAAGCAGCTGTATGAAGTGCCGCCCCGGTTCATTGGGCAATCGATTGAACTCCGCTATGACGAACGGGGCGTCTATGTGTACGA
AGATGGAAGGAAGGTGGCGGAGGCCGTTTGGGTTCGTTTGGAGGACAACGCCTATGTAAAACGCCATCGGTCCCCGTTTGCGGCGATTCCGGCGAAGGAG
GGAGAACACGGTGTATAAATCGTTTTACTCCCTTTCGCGGGAGCCGTTTGCGAAAGAAACA
GGTACGATAAGGGCAGACGGACCGGAACGGTCCTGATTCCAAGTGAGACAAGGAGGAGATTCGAATGGATGAGTCGATGAGACACGACATTGCCCTGTTT
CGGTACGGGCTAATCGCTCCGTTGGTGAATGGGCAGGTGGAACCGAAGGCGTATCTGAAAGAGGTGAGCGAGCGAGTGCACCATGTTCCCCACCAAGGCG
ACAAACGAATCGCAGCCAAGACGATCCTCGATTGGTGCACCCGATACAAAAAAGGGGGCTTTGACGCCTTAAAGCCGAAGCGCCGTTCGGACCGCGGCCA
CTCAAGGCGTCTGTCGCCCGATGATGAGGATCATATTTTAGCCCTAAGAAAAGAACATCCCACCATGCCCGTGACGGTGTTCTACGAACACCTGATCGAG
CAGGGGGAAATCCCAAAAAACCATATCTCTTACTTTACTATATACCGATTGTTGAAAAAACACAACCTTGTCGGGAAAGAAATCTTGCCCATGCCGGAGC
GAAAGCGCTTTGCGTATGACCAGATCAATGAGCTTTGGCAAGGGGACTTATCCCATGGACCAACGATTCGAGTCAATGGGAAAGCCCAGAAAACGTTTTT
GATCGCCTATATCGATGACTGCTCGCGGTTTGTGCCCGTAACGTATAGTTTCCCTTCGGAGAAGTTTGACGGGCTGCGGATCGTCACGAAGGAAGCGGTG
CTTCGTTGCGGGAAGCCGAAGCGCATTTACTCGGACAACGGGAAAATTTATCGATCCGAGGTGCTGCAGTATGCGTGCGCCGAGATGGGAATTACGCTCA
TCCACACCCAGCCGTATGACCCGCAAAGCAAAGGGAAAATCGAACGGTTCTTCCGCACCGTACAGACGCGGTTTTATCCGCTGATGGAGTTGGATCCCCC
GAAGTCGCTCGAATACCTGAACGAGCGTTTTTGGAGGTGGCTTGAAGAGGAGTATCATCGAAAACCGCACGCCTCGCTGGACGGAAAAACGCCGCATGAG
GTGTTTCAATCGCAAGTGCATCTCGTGTCGTTCATCGAAGATGGCGATTGGCTGGATGCGATTTTTCTGAAGCGGGAGCAACGCAAGGTCAAAGCCGATG
GAACGATCACCTTGAACAAGCAGCTGTATGAAGTGCCGCCCCGGTTCATTGGGCAATCGATTGAACTCCGCTATGACGAACGGGGCGTCTATGTGTACGA
AGATGGAAGGAAGGTGGCGGAGGCCGTTTGGGTTCGTTTGGAGGACAACGCCTATGTAAAACGCCATCGGTCCCCGTTTGCGGCGATTCCGGCGAAGGAG
GGAGAACACGGTGTATAAATCGTTTTACTCCCTTTCGCGGGAGCCGTTTGCGAAAGAAACA
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1254 bp | 417 aa | 165 | 1418 | + | No |
Chemistry : DDE
ORF sequence :
MDESMRHDIALFRYGLIAPLVNGQVEPKAYLKEVSERVHHVPHQGDKRIAAKTILDWCTRYKKGGFDALKPKRRSDRGHSRRLSPDDEDHILALRKEHPT
MPVTVFYEHLIEQGEIPKNHISYFTIYRLLKKHNLVGKEILPMPERKRFAYDQINELWQGDLSHGPTIRVNGKAQKTFLIAYIDDCSRFVPVTYSFPSEK
FDGLRIVTKEAVLRCGKPKRIYSDNGKIYRSEVLQYACAEMGITLIHTQPYDPQSKGKIERFFRTVQTRFYPLMELDPPKSLEYLNERFWRWLEEEYHRK
PHASLDGKTPHEVFQSQVHLVSFIEDGDWLDAIFLKREQRKVKADGTITLNKQLYEVPPRFIGQSIELRYDERGVYVYEDGRKVAEAVWVRLEDNAYVKR
HRSPFAAIPAKEGEHGV
MPVTVFYEHLIEQGEIPKNHISYFTIYRLLKKHNLVGKEILPMPERKRFAYDQINELWQGDLSHGPTIRVNGKAQKTFLIAYIDDCSRFVPVTYSFPSEK
FDGLRIVTKEAVLRCGKPKRIYSDNGKIYRSEVLQYACAEMGITLIHTQPYDPQSKGKIERFFRTVQTRFYPLMELDPPKSLEYLNERFWRWLEEEYHRK
PHASLDGKTPHEVFQSQVHLVSFIEDGDWLDAIFLKREQRKVKADGTITLNKQLYEVPPRFIGQSIELRYDERGVYVYEDGRKVAEAVWVRLEDNAYVKR
HRSPFAAIPAKEGEHGV
Blast result :
Comments
ISBst1 is 43% aa similar to ISA0963-5, 44% to ISVch1, 42% to Tn552, 38% to ISSco1.
Note that the Irs terminate with TGT but are not typical of the familly. In addition, the ORF is longer by about 100 aa than expected.
Note that the Irs terminate with TGT but are not typical of the familly. In addition, the ORF is longer by about 100 aa than expected.
References
1] Gat,O., Lapidot,A., Alchanati,I., Regueros,C. and Shoham,Y.(1994) Appl. Environ. Microbiol. 60 (6), 1889-1896
2] Shulami,S., Gat,O., Sonenshein,A.L. and Shoham,Y. (1999) J. Bacteriol. 181 (12), 3695-3704
3] Shulami,S., Gat,O. and Shoham,Y.(1999) Direct Submission
2] Shulami,S., Gat,O., Sonenshein,A.L. and Shoham,Y. (1999) J. Bacteriol. 181 (12), 3695-3704
3] Shulami,S., Gat,O. and Shoham,Y.(1999) Direct Submission