ISBs2
- Family IS630
- Group
Isoform Synonym(s) RSBst-beta
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NZ_ACWF01000061 | ND | Bacillus stearothermophilus | Bacillus stearothermophilus CU21 Bacillus smithii |
DNA section
IS Length : 1133 bp
Ends
IR Length : 14/17
IRL : AATTGAAATAAAGGTTTTCTCCCGTTCCACGGCGAATAAATACAAAGAGA
IRR : AGTTGAATTAAAGATTTACAACTGAACACACAAACGATCGATCGTTTGTT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| TTAATATATA | TA | TATAGATGCA | 2 |
DNA sequence
AATTGAAATAAAGGTTTTCTCCCGTTCCACGGCGAATAAATACAAAGAGAAAATAGGGGAACGGAGAGAAAATATGATGCCAAATCACAAAGACGAGATC
GAAAAGTTGTCCACTGCCATGAAAGAAGCAAAAAGTAAACGAGCATATGAACGCTACCAAGCGATTTATCTTCATTTGCAGGGATATACCAAAGGAGAAA
TCGCAACGATTATTGGTCGATCCAAGAAAACTATTTATAACTATATTCATGCCTACGCCCAGCGCGGTCTTGATGGACTGGAGATGAAATACTCACCTGG
CGCCCCACGTCGATTGACCCCTGAGCAGGAAAAAGAGCTGGCTTTGATCATTGAACATCAGCTCCCCGTAGATGTGGGATTCGAAGCAAAATATAATTGG
ACGCTTGCGATAATCGCTGAACTCATTCAACAAAAGTGGGGGCCAACATACACGCTTCGCGGAACAAGTGACATTCTGCATCGGTTAGGGCTAAGCTATA
CGAAACCGACCTATACACTAGCCAATGCCGATGAAGAGAAACAAAAAGAATTCGTCGAAATCACCTTCCCTGAAGTAAAAAAAACTGGTAGATGGGAACA
TCGCCCATGTCCTCTTTCAAGATGAGTCGATGATTCGTGATTACCAAGCGATTCAAAAAACATGGTTTGTCAAAGGAAAACAACGAATCATTCCGACGTT
TGGAAAACATCAAGGGCTGAAGCTGATTGGCACGTTGAACTACGAAACAGGGGAAGTGTTTTGCATCGAAGAAGAATGCTATGACGCGGAAACATTTCTT
CGATTTCTTCAACTTGTGTTAGAACGCTATCCCACAGGCAAAATAGTGATGATTTTAGATAACGCTCGAATTCACCATGCCAAACTCATTCAGCCATTTT
TAAAAGAACACGAAGATCGGTTAGAGCTCGTCTTTTTGCCACCATACAGTCCGCAATTGAACTTGATTGAAGGGCTATGGAAATGGCTGAAATCAGACGT
GATTTACAACGTGTTCTATTCGAGTGTGCAAGAAATTAGAAAGAATGTCCAAGCCTTTATTCAGCGAATCAACCAGAAACCAGAACAAACGATCGATCGT
TTGTGTGTTCAGTTGTAAATCTTTAATTCAACT
GAAAAGTTGTCCACTGCCATGAAAGAAGCAAAAAGTAAACGAGCATATGAACGCTACCAAGCGATTTATCTTCATTTGCAGGGATATACCAAAGGAGAAA
TCGCAACGATTATTGGTCGATCCAAGAAAACTATTTATAACTATATTCATGCCTACGCCCAGCGCGGTCTTGATGGACTGGAGATGAAATACTCACCTGG
CGCCCCACGTCGATTGACCCCTGAGCAGGAAAAAGAGCTGGCTTTGATCATTGAACATCAGCTCCCCGTAGATGTGGGATTCGAAGCAAAATATAATTGG
ACGCTTGCGATAATCGCTGAACTCATTCAACAAAAGTGGGGGCCAACATACACGCTTCGCGGAACAAGTGACATTCTGCATCGGTTAGGGCTAAGCTATA
CGAAACCGACCTATACACTAGCCAATGCCGATGAAGAGAAACAAAAAGAATTCGTCGAAATCACCTTCCCTGAAGTAAAAAAAACTGGTAGATGGGAACA
TCGCCCATGTCCTCTTTCAAGATGAGTCGATGATTCGTGATTACCAAGCGATTCAAAAAACATGGTTTGTCAAAGGAAAACAACGAATCATTCCGACGTT
TGGAAAACATCAAGGGCTGAAGCTGATTGGCACGTTGAACTACGAAACAGGGGAAGTGTTTTGCATCGAAGAAGAATGCTATGACGCGGAAACATTTCTT
CGATTTCTTCAACTTGTGTTAGAACGCTATCCCACAGGCAAAATAGTGATGATTTTAGATAACGCTCGAATTCACCATGCCAAACTCATTCAGCCATTTT
TAAAAGAACACGAAGATCGGTTAGAGCTCGTCTTTTTGCCACCATACAGTCCGCAATTGAACTTGATTGAAGGGCTATGGAAATGGCTGAAATCAGACGT
GATTTACAACGTGTTCTATTCGAGTGTGCAAGAAATTAGAAAGAATGTCCAAGCCTTTATTCAGCGAATCAACCAGAAACCAGAACAAACGATCGATCGT
TTGTGTGTTCAGTTGTAAATCTTTAATTCAACT
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 552 bp | 183 aa | 74 | 625 | + | No |
Description : First part of the transposase
ORF sequence :
MMPNHKDEIEKLSTAMKEAKSKRAYERYQAIYLHLQGYTKGEIATIIGRSKKTIYNYIHAYAQRGLDGLEMKYSPGAPRRLTPEQEKELALIIEHQLPVD
VGFEAKYNWTLAIIAELIQQKWGPTYTLRGTSDILHRLGLSYTKPTYTLANADEEKQKEFVEITFPEVKKTGRWEHRPCPLSR
VGFEAKYNWTLAIIAELIQQKWGPTYTLRGTSDILHRLGLSYTKPTYTLANADEEKQKEFVEITFPEVKKTGRWEHRPCPLSR
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 540 bp | 179 aa | 579 | 1118 | + | No |
Description : Second part of the transposase
ORF sequence :
KKLVDGNIAHVLFQDESMIRDYQAIQKTWFVKGKQRIIPTFGKHQGLKLIGTLNYETGEVFCIEEECYDAETFLRFLQLVLERYPTGKIVMILDNARIHH
AKLIQPFLKEHEDRLELVFLPPYSPQLNLIEGLWKWLKSDVIYNVFYSSVQEIRKNVQAFIQRINQKPEQTIDRLCVQL
AKLIQPFLKEHEDRLELVFLPPYSPQLNLIEGLWKWLKSDVIYNVFYSSVQEIRKNVQAFIQRINQKPEQTIDRLCVQL
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1045 bp | 347 aa | 74 | 1118 | + | Yes |
Chemistry : DDE
ORF sequence :
MMPNHKDEIEKLSTAMKEAKSKRAYERYQAIYLHLQGYTKGEIATIIGRSKKTIYNYIHAYAQRGLDGLEMKYSPGAPRRLTPEQEKELALIIEHQLPVD
VGFEAKYNWTLAIIAELIQQKWGPTYTLRGTSDILHRLGLSYTKPTYTLANADEEKQKEFVEITFPEVKKLVDGNIAHVLFQDESMIRDYQAIQKTWFVK
GKQRIIPTFGKHQGLKLIGTLNYETGEVFCIEEECYDAETFLRFLQLVLERYPTGKIVMILDNARIHHAKLIQPFLKEHEDRLELVFLPPYSPQLNLIEG
LWKWLKSDVIYNVFYSSVQEIRKNVQAFIQRINQKPEQTIDRLCVQL
VGFEAKYNWTLAIIAELIQQKWGPTYTLRGTSDILHRLGLSYTKPTYTLANADEEKQKEFVEITFPEVKKLVDGNIAHVLFQDESMIRDYQAIQKTWFVK
GKQRIIPTFGKHQGLKLIGTLNYETGEVFCIEEECYDAETFLRFLQLVLERYPTGKIVMILDNARIHHAKLIQPFLKEHEDRLELVFLPPYSPQLNLIEG
LWKWLKSDVIYNVFYSSVQEIRKNVQAFIQRINQKPEQTIDRLCVQL
Blast result :
Comments
ISBs2 is 48% aa similar to ISAzo32. The third ORF is the putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
September 20 2012 : the partial sequence was replaced by the most closely complete sequence (99% of DNA identity).
Old accession number : Z21626.
Previous comments : ISBs2 is located upstream of the glgB (glycogen branching enzyme) gene in strain CU21 of Bacillus stearothermophilus (Kiel et al., 1993). The left end of ISBs2 is purely speculative and needs confirmation.
September 20 2012 : the partial sequence was replaced by the most closely complete sequence (99% of DNA identity).
Old accession number : Z21626.
Previous comments : ISBs2 is located upstream of the glgB (glycogen branching enzyme) gene in strain CU21 of Bacillus stearothermophilus (Kiel et al., 1993). The left end of ISBs2 is purely speculative and needs confirmation.
References
1] Kiel, J.A.K., Boels, J.M., Ten Berge, A.M., and Venema, G. (1993) DNA Sequencing Mapping 4, 1-9.
2] Earl,A., Ward,D., Feldgarden,M., Gevers,D., Daigneault,M., Strauss,J., Allen-Vercoe,E., Young,S.K., Zeng,Q., Gargeya,S., Fitzgerald,M., Haas,B., Abouelleil,A., Alvarado,L., Arachchi,H.M., Berlin,A., Brown,A., Chapman,S.B., Chen,Z., Dunbar,C., Freedman,E., Gearin,G., Goldberg,J., Griggs,A., Gujja,S., Heiman,D., Howarth,C., Larson,L., Lui,A., MacDonald,P.J.P., Montmayeur,A., Murphy,C., Neiman,D., Pearson,M., Priest,M., Roberts,A., Saif,S., Shea,T., Shenoy,N., Sisk,P., Stolte,C., Sykes,S., Wortman,J., Nusbaum,C. and Birren,B. (2011) Direct submission GenBank.
2] Earl,A., Ward,D., Feldgarden,M., Gevers,D., Daigneault,M., Strauss,J., Allen-Vercoe,E., Young,S.K., Zeng,Q., Gargeya,S., Fitzgerald,M., Haas,B., Abouelleil,A., Alvarado,L., Arachchi,H.M., Berlin,A., Brown,A., Chapman,S.B., Chen,Z., Dunbar,C., Freedman,E., Gearin,G., Goldberg,J., Griggs,A., Gujja,S., Heiman,D., Howarth,C., Larson,L., Lui,A., MacDonald,P.J.P., Montmayeur,A., Murphy,C., Neiman,D., Pearson,M., Priest,M., Roberts,A., Saif,S., Shea,T., Shenoy,N., Sisk,P., Stolte,C., Sykes,S., Wortman,J., Nusbaum,C. and Birren,B. (2011) Direct submission GenBank.