ISBs1
- Family IS982
- Group
Isoform Synonym(s) RSBst-alpha
Accession number | Transposition | Origin | Host |
---|---|---|---|
Z21626 | ND | Bacillus stearothermophilus | Bacillus stearothermophilus CU21 |
DNA section
IS Length : 996 bp
Ends
IR Length : 18/21
IRL : ACCCGTGGTGCTAGATAAACTCAAACAAGCATAAAAATAGCCCTTGCATG
IRR : ACCCGTGGTGCTAGTTGAGCTTTAGCGCTCAACTAGCCCAAGTGTAACCA
Insertion site
Left flank | Direct repeat | Right flank | DR Length |
---|
DNA sequence
ACCCGTGGTGCTAGATAAACTCAAACAAGCATAAAAATAGCCCTTGCATGAGGATCCCCTTAAAATGAAAGTGCAACCAACCATTAAAAAGGAGGATCCC
TATGCAAGAGCACTTTCATTTTACTACAGATCGAGCCAAGATTCAAAAGCAATATGCCGCCATTTTCGTTTTTGTTTCTGCTCAACTTTCATGCATTCAG
GTGCATCTTCATCGTCGAAATCGCCATTTGGTCAAGCAAGAGGACGCTGTCATCATCGCCATTCATCTTTTAGGAAAGCTGCTCGGTTTTACTTCACGGG
CATGGCATCGTTTTGTCACGGGAAATTTGTTCACAAACGTCTCGTTTCTCGAACGTTCCCGGTATAACCGCCGCTGCCGGGCGCTTGGCTTCGCTATCAA
ATGGATTGCCGTCGTGAGCAAAACGCGGCCAACATCATGCCTATGCCGTCGTGGACAGCTTGCCGCTCCCGTTGTGCCATACGGCAAGAATGCATCGCGT
CAAACGGTTTCAAGAGATCGCCGACATCGGGTATTGCGCTTCCAAAAAGCAATGGTACTACGGGTTGAAGCTGCACCTTCAAGTGACCGATCAAGGGCTG
CCAATGGGGTATGTGGTGACGGAAGCATCTTGCCACGATCGAATCGCAGCCGAAAGCGTGATGACCCAAATTCCCCACCCGTATAACTTTGGGGACAAAG
GATTCATTAGCCGTGACTTGCAAAAAAGGCTGTACGAAGAATACCAAATGGCGCTTTGGACTCCGTCTCGAAAAAACCAGAAACATCGTGCGTCCGAGGC
ATGGGAGAAATGGATCCAACAAAAACGCAAAGTGATCGAGACGGTGTTCTCGGTTCTGGTTGACCACTATCGCATCACGGGGATCCGAGCCAATTCGATT
ATCGGATTTGAAGTGGCACTAGACGGTATATTGTTAGCTTATTCCCTGGTTACACTTGGGCTAGTTGAGCGCTAAAGCTCAACTAGCACCACGGGT
TATGCAAGAGCACTTTCATTTTACTACAGATCGAGCCAAGATTCAAAAGCAATATGCCGCCATTTTCGTTTTTGTTTCTGCTCAACTTTCATGCATTCAG
GTGCATCTTCATCGTCGAAATCGCCATTTGGTCAAGCAAGAGGACGCTGTCATCATCGCCATTCATCTTTTAGGAAAGCTGCTCGGTTTTACTTCACGGG
CATGGCATCGTTTTGTCACGGGAAATTTGTTCACAAACGTCTCGTTTCTCGAACGTTCCCGGTATAACCGCCGCTGCCGGGCGCTTGGCTTCGCTATCAA
ATGGATTGCCGTCGTGAGCAAAACGCGGCCAACATCATGCCTATGCCGTCGTGGACAGCTTGCCGCTCCCGTTGTGCCATACGGCAAGAATGCATCGCGT
CAAACGGTTTCAAGAGATCGCCGACATCGGGTATTGCGCTTCCAAAAAGCAATGGTACTACGGGTTGAAGCTGCACCTTCAAGTGACCGATCAAGGGCTG
CCAATGGGGTATGTGGTGACGGAAGCATCTTGCCACGATCGAATCGCAGCCGAAAGCGTGATGACCCAAATTCCCCACCCGTATAACTTTGGGGACAAAG
GATTCATTAGCCGTGACTTGCAAAAAAGGCTGTACGAAGAATACCAAATGGCGCTTTGGACTCCGTCTCGAAAAAACCAGAAACATCGTGCGTCCGAGGC
ATGGGAGAAATGGATCCAACAAAAACGCAAAGTGATCGAGACGGTGTTCTCGGTTCTGGTTGACCACTATCGCATCACGGGGATCCGAGCCAATTCGATT
ATCGGATTTGAAGTGGCACTAGACGGTATATTGTTAGCTTATTCCCTGGTTACACTTGGGCTAGTTGAGCGCTAAAGCTCAACTAGCACCACGGGT
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 1
ORF 1
Length | Begin | End | Strand | Fusion ORF | |
---|---|---|---|---|---|
874 bp | 290 aa | 102 | 975 | + | Yes |
Chemistry : DDE
ORF sequence :
MQEHFHFTTDRAKIQKQYAAIFVFVSAQLSCIQVHLHRRNRHLVKQEDAVIIAIHLLGKLLGFTSRAWHRFVTGNLFTNVSFLERSRYNRRCRALGFAIK
WIAVVSKTRPTSCLCVVDSLPLPLCHTARMHRVKRFQEIADIGYCASKKQWYYGLKLHLQVTDQGLPMGYVVTEASCHDRIAAESVMTQIPHPYNFGDKG
FISRDLQKRLYEEYQMALWTPSRKNQKHRASEAWEKWIQQKRKVIETVFSVLVDHYRITGIRANSIIGFEVALDGILLAYSLVTLGLVER
WIAVVSKTRPTSCLCVVDSLPLPLCHTARMHRVKRFQEIADIGYCASKKQWYYGLKLHLQVTDQGLPMGYVVTEASCHDRIAAESVMTQIPHPYNFGDKG
FISRDLQKRLYEEYQMALWTPSRKNQKHRASEAWEKWIQQKRKVIETVFSVLVDHYRITGIRANSIIGFEVALDGILLAYSLVTLGLVER
Blast result :
Comments
ISBs1 is interrupting the glgB (glycogen branching enzyme) gene in strain CU21 of Bacillus stearothermophilus (Kiel et al., 1993). A fusion between the two ORFs (103-449 and 449-976) is considered here. This fusion is simply based on the homology with the IS982 transposase (Yu et al., 1995). This suggestion remains to be confirmed. As for IS982, the limits of ISBs1 were deduced from comparison between all the IS982 family members.
References
1] Kiel, J.A.K., Boels, J.M., Ten Berge, A.M., and Venema, G. (1993) DNA Sequencing Mapping 4, 1-9.
2] Yu, W., Mierau, I., Mars, A., Johnson, E., Dunny, G., and McKay, L.L. (1995) Plasmid 33, 218-225.
2] Yu, W., Mierau, I., Mars, A., Johnson, E., Dunny, G., and McKay, L.L. (1995) Plasmid 33, 218-225.