ISfinder Mirror Site

ISCausp4

Family IS1595
Group ISNha5

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
AKKF01000138	ND	Caulobacter sp.	Caulobacter sp. AP07

DNA section

IS Length : 3070 bp

Ends

IR Length : 24

IRL : CGGTATTATGTAGCAAACACACCTGCTGCGATGCCGGGTCTCTAGAGGAG
IRR : CGGTATTATGTAGCAAACACACCTAGCCCAGCATCGTTCAGAAGTGAATG

Insertion site

Left flank	Direct repeat	Right flank	DR Length
CACCAGCAAC	CACTTCCG	CGCCGAAGCC	8

DNA sequence

CGGTATTATGTAGCAAACACACCTGCTGCGATGCCGGGTCTCTAGAGGAGACCTGAGTTGACGAAACAGCCTCACGGTTGCGCTTCTGCGGGACTCCCGG
TCATTGTCTCGGGATGGGTGCGCCAGCCTCGGCATACGAGAAAATCACGGCCATCACGACGGGCGCGTTGGCGTTCGCGGTTAGCGGCGTGCTGACTGTT
CTCGATCACATGGGAGTCGAAGTCCCCAATTGGGCTTGGATGGCCTACGGCTGGGGATGCGGGTTCCTGGCGCTCGTGTGTGTCTCGCTGTCGATTCACA
TGGTCTTGGAGGGGGCGTTCAGCCGCAAGCGCCCCAAGGCTCCACCGTTGGTCCTAGAGCCCGTCACGCCGCCCGATCCCCAGGCGGCGCTTCTTGAGGT
GCAAGAGGCGATTGCGAATGAGCTATGGCTTCAGAGAGCGCATCGCGAGGGGGCTGACCGCCTGACGCGGGCAAGCTGGATGGTGTTCGGAAGACCCCCT
CACGAAATCAATCCAGCCGAAGAGTCGTCACCCCGATTGCCTGCAAAGCGGCGAGGAAGAACCAAGCGGGGAAGCCGCCCCGATTGATCTTGGCTTGAAC
TCCCGTCTCGGTCTCGGGAAGCCCCATCTCAGACAGCTTACGCGCCAGGTCGGCATTCTTCATGCCTGCCCGGACCATCTCGGCTTTCAGATGGCGCTTG
GCCTTGTCGGCCCATTCGTCGCTAGTCGCGGCCATCCGATTTTCTCCACTTGTGAATGCCAATCAAACATATCTGTCGCTTTTCCCATTGCAAGCGTCGA
TGGCATTTCCTATCTATCCGTTGTCGAACCATACGGATATGTCGGTTGTCACAACACTTCCTCCTCTCTGCCGCCGCCCGGACCCTGTCGCTCGGGAAGG
TGATGCGCATGTCGGAAGACGAGGCGTTCGAGACGTTCAAGACCATCCGTTGGGACGAGAACGAGGGTCAGCCCTATTGCGGCAAGTGCGGTTGCACGGC
CGTCTACACGTTCAAGGCTCGTCGCATCTTCAAGTGCAAGAGCTGCGAGGCTCAGTTCTCGGTGACGAGCGGGACCATCTTCGCCAGCCGCAAGCTCGCC
GTGCGCGACATTCTCGCGGCTATCGCGATCTTCGCCAACGGCGCCAAGGGCTATAGCGCCCTCCAATTGAGCCGGGATCTCGACGTCCAGTACAAGACGG
CGTTCGTGATGGCGCACAAGCTGCGCGAAGCCCTGGGCAAGGTGGCGGACCGGACCAAGCTCGATGGCGTGGTCGAGATCGATGGCGCCTACTTCGGCGG
CTACGTGAAGCCCGCGAACGAGAAGTGGGCTCGTCAGGACCGGCGCCTCCTCGAAAACCAGACGGGTAAGCGCCAGGTTGTCGTGGTGTTGCGCGAGCGT
GGCGGCCGGACGTTGCCGTTCGTCACCAAGGCTGAGCGTGAGGGTGTCCCCCTGGTGACCGCCAACGTGTCCCCGGGGACAACCGTCCACGCGGACGAGG
CTCCGCATTGGGACGTCCTGTCGGCCAAGTTCGCGACCAAGCGGATCAACCACTCGGAGGCCTATAGCCTCGATGGCGCCTGCACCAACTGGGCCGAGAG
CTTCTTCAGCCGCATCCGCCGGGCCGAAGCCGGCGTCCATCACAAGATCGCCGGCCGCTATCTGGAAGCCTATGCGGGCGAAATGGCGTGGCGCGAGGAT
CACCGCCGCATGAGCAATGGTGTCCAGTTCGCCCAGATCGTCGATGCGGCCATGTCGGCCCCCGTCTCGCGCCAGTGGAAGGGCTATTGGCAACGGCCCG
CTTAGGGCGGATAAAACACATGCCTCGTCCGAAGTTTGGTCACGGAAGACGAGGTGCGCTGTTGCGCGTGAACGCGGGCCCGCTCTGTCGGGCGGCCTGC
TCAATAAAATACCTTCGGGGAATAAGCAGGACGCGCTTCCCCGTGTTCGCGCGTGACCAACCGTCCCGGCACTCCGCCGGGCTTGGGAGCACGGGGGACT
ATGAGGAATCACGTCTGTATGGGGATGGCGCTTGCCATCGCCTTGAGCGCTGTCACGTCGATAGCTCACGCTCAGACCGCCACGACGCCGGGTGTGACGC
CCGAGACGGGAAAGTGGCGCTACACCGAAACGGCCTCCGCCCTGGACGGAGCTAAGAGCCGGATCGCCACCCTGACGGCGGAGACGCAAGTCGCGAACAT
CTTGGGCCGCATGGAGGCTCCGACCTTGGGCCTCACCTGCGACAAGAACGGCCTGGGCGTGGTCATGTCGTGGCCCGACTTCGTGGGCGAGGCCGGATTC
CTGACGCTTCCGGTCAAGTGGAAGATCGATGACGGCAAGGTCTACAAGACCGGGTGGTTCCCGGGGACCACGAGCGTCACTCTCATGGGCCCAGGAGCCC
AGGGATGGATAAGGCAGGTCAAGGACGCCAAGACCCTCGTGGTCGCCGTCCCTGATCGCCACGGAGGCCAGGAGGCCACCTTCGACCTCACGGGGATCGA
GGCCATCGGCCCGCGCTTCTCCGAGGTGTCCTGTGGCGGCGGAGCTGGCGCGCGCTAGACCGCCAGCCGCCAGACGAATTCACACCGCTTGGTGTCGTTC
TCCGAGATCGCAAGGCCTGCGTCGCGCATCTGGCGTAGCGCCTTGCTGATCCGCCGGACGACGTCGAGCATCATCCGGCGGTCCCGGCCGTCCTTGCCCT
CATAGTTGATGATCGTCAGGGCGAGTTGCCGGGCGGTCTGCGGCCCCTTCTCGCGAAGCTGGCCAATGAGGAACTGGCGGAGCTCGCCCCGGTAGAACAG
GACGATGCGCGGCGCCCTGACGGTCAGCCGGACCTCGCCGTCATACCCCAGGGTCTCTAGGACCCGGTCGACCGCCTCGACGTCGTTCGAGAGAACCGCC
AGCCTCTCCCGAGTGATGGCGATCTCCTCCATCATCTCCTCCCGCTTCTTGAGCAAGCCGGAGATGGCGTGAGCGAAAGTCTCGGTTCTGGCGGGTTTCA
TGCCCCAGAACGTACAAAATCATTCACTTCTGAACGATGCTGGGCTAGGTGTGTTTGCTACATAATACCG

Protein section

ORF number : 4

ORF 1

Length		Begin	End	Strand	Fusion ORF
228 bp	75 aa	735	508	-	No

ORF function : Passenger Gene
Annotation : Hypothetical proteinDescription :

ORF sequence :

MAATSDEWADKAKRHLKAEMVRAGMKNADLARKLSEMGLPETETGVQAKINRGGFPAWFFLAALQAIGVTTLRLD

Blast result :

ORF 2

Length		Begin	End	Strand	Fusion ORF
903 bp	300 aa	903	1805	+	No

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MRMSEDEAFETFKTIRWDENEGQPYCGKCGCTAVYTFKARRIFKCKSCEAQFSVTSGTIFASRKLAVRDILAAIAIFANGAKGYSALQLSRDLDVQYKTA
FVMAHKLREALGKVADRTKLDGVVEIDGAYFGGYVKPANEKWARQDRRLLENQTGKRQVVVVLRERGGRTLPFVTKAEREGVPLVTANVSPGTTVHADEA
PHWDVLSAKFATKRINHSEAYSLDGACTNWAESFFSRIRRAEAGVHHKIAGRYLEAYAGEMAWREDHRRMSNGVQFAQIVDAAMSAPVSRQWKGYWQRPA

Blast result :

ORF 3

Length		Begin	End	Strand	Fusion ORF
540 bp	179 aa	2019	2558	+	No

ORF function : Passenger Gene
Annotation : Hypothetical proteinDescription :

ORF sequence :

MGMALAIALSAVTSIAHAQTATTPGVTPETGKWRYTETASALDGAKSRIATLTAETQVANILGRMEAPTLGLTCDKNGLGVVMSWPDFVGEAGFLTLPVK
WKIDDGKVYKTGWFPGTTSVTLMGPGAQGWIRQVKDAKTLVVAVPDRHGGQEATFDLTGIEAIGPRFSEVSCGGGAGAR

Blast result :

ORF 4

Length		Begin	End	Strand	Fusion ORF
447 bp	148 aa	3001	2555	-	No

ORF function : Passenger Gene
Annotation : Hypothetical proteinDescription :

ORF sequence :

MKPARTETFAHAISGLLKKREEMMEEIAITRERLAVLSNDVEAVDRVLETLGYDGEVRLTVRAPRIVLFYRGELRQFLIGQLREKGPQTARQLALTIINY
EGKDGRDRRMMLDVVRRISKALRQMRDAGLAISENDTKRCEFVWRLAV

Blast result :

Comments

ISCausp3 is 79% (transposase) aa similar to ISNwi4.

References

1] ISfinder annotation (2017)
2] Brown,S.D., Utturkar,S.M., Klingeman,D.M., Johnson,C.M., Martin,S.L., Land,M.L., Lu,T.Y., Schadt,C.W., Doktycz,M.J. and Pelletier,D.A. (2012) J. Bacteriol. 194 (21), 5991-5993 .