ISfinder Mirror Site

ISBos1

Family IS1595
Group ISNha5

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
CP014301	ND	Bosea sp.	Bosea sp. PAMC 26642

DNA section

IS Length : 3731 bp

Ends

IR Length : 22/23

IRL : CGGCATTAGGTAGCAAGCTCACCAACTGCTGAGTCCGTTAACCATAGAGT
IRR : CGGCATTGGGTAGCAAGCTCACCTAAGAGAACGTATGCCAACGTTTTCCG

Insertion site

Left flank	Direct repeat	Right flank	DR Length
TGTGCGCCCT	GGATTATC	CGCCGGCGAA	8

DNA sequence

CGGCATTAGGTAGCAAGCTCACCAACTGCTGAGTCCGTTAACCATAGAGTGCCTGTGGATAACTTTCTGAGACTTCCCAATCTTGGCTTGGGCGCTTGCC
TTGGCTGCCGACTCGACGTATGGGTTGACTCAAGGAGAGGCAGCCATGACTACGCTCCAAGAATTCTTACACGGCCGGATGAACGAGCTTGCAACCGACG
ATGCTAGCTTAAGGCGCAAGCTTGCGCTCAATGAACAAGAGCGAGAGCAGCTTCAAAAGGCTGCAAAAGCGGCAGGGCTGCACCTTAAGCCCATTACCGA
AGCGCCGCCACCGCCGCCGGCTACTTATGTCTTGTCGGTCGGAACCTCCCAAGGGCGCGCGACTACCCAAGGGGTAGGCATGACAGTCTCGCGGCGGCCA
ATCCCCGAGAAGACGATTAAGGAAGCTGTGTTGGAAGTCCTTGGGGTTCTAGGCACAGGCCTTACAGCGTTGGATCTGTTGTCGGCCATCAACGCCAAAT
TCGATACTGACTACCCTAGGACTTCGCTGAGCCCGCAGTTGTCGCGGCTGAAGGCAGAGGGGAAAATTACCCGCTTGGGTAACTTGTGGAGTTTGGCGCC
CGATGCGCCAGAAACGAATGAGCCCGCTCATCCAACGTCCGAGGGGACGAATGAGCGGGCTCAGGTTCCTGAAACCAACAGCACGACCGTGGAGCCGGTC
GGGGAGGTGGCACATGAGAAGACGTTGACCGTCGACCCACTTGAATAACCACGCGGCGGCTCGGGTGTTCGCGCATCCGGGTCGCTCTGCGGGGGTAGAT
GGTCGGGCTAACGGTCTCCCACCAGCGAGCCCAGGGGTTCGTGTCCCCTTACCTCCACCACAGCGCCCGCTTTGCAGGTAACTGCAAAGTGGGCGCCCTT
TATATAGCGAACCTAGGCAGGGGCGTCTAGCGCCTCAACGCTTTTCGTTGGCGGGGCGTAAGTGATTCCACTCGCTGTGAAATTGATCCCGCCGCCTTCG
AGGGCGCGCCTCATCGCGTTTAAATTATTGGCGATTGGGACGCGACGCGCCTTCTCATAATCCCGCACGGTCGAAAGCGATACGCTTGCAGCGCTCGCTA
GCTGATCTTGCGTCCAATCCAACCACCCGCGCGCGGCGCGGCTCTGTTCTGGCGACATACACGATGACTAGTGACAAAAAGTTTTTCAGTCAACCCCAAC
GAAAAGTGTTGACGTCGCTGATTTCCATGGATATCACTGATGTCAACGATATTCGTTGGCGTGGGCATGGCACAGCATTTCCTCCTTTCCGCAGCGGCCC
GCACCCTCTCTTTGGTGACGGTCGCCCGCATGTCGGACGACGAAGCGCATAGCGCCTTTCGCCAGATCCGTTGGTGCGACACTGGCGGCGAGCCGGTTTG
CCCGCGTTGCAACTGCGGTGCGGTCTACACGTTCAAGACCCGCAAGCTCTACAAGTGCAAAGCCTGCACGCACCAGTTCTCGGTCACGTCTGGTACGATC
TTCGCCAGCCGCAAACTGGCTATCCGCGACTACCTCCTGGCCATCGCGATCTTCGTCAACGGCGCCAAGGGCCATTCGGCCTTGCAGCTCTCCCGCGACA
TCAACGTCCAGTACAAGACGGCCTTCGTCTTGACGCACAAGCTGCGCGAGGCGATGTCCGCCGAAATGGCCGACATGACTGTCTCGGGTGAGGTCGAGAT
CGACGGCGCCTATTTCGGTGGTCACGTTCGCCCGGCCAACTTCAAAGAGAACCGCGTCGATCGCCGTCTCGCCAAGAACCAGAACGGCAAGCGCCGCGTC
GTGGTCATCATGCGCGAGCGCGCCGGTCGCACGTTGCCATTCGTATTTAAGTCTGAGGGCGCCTCGCTGGCGACCATCGGCCGACGCGTGCACCCCTCGG
CTACGGTTCACGCTGACGAGGCGTTGCATTGGGATGAGCTTCACACGTTCTATCTGACGAAGCGCATCAACCACAGCGAGGCCTATTCGGACGGCCAATC
CTGCACCAACATGGCGGAAAGCTTTTTCTCCCGCCTGCGTCGCGCCGAGATCGGCACGCATCACCACATCGCCGGCCCGTACCTCAACGCCTACTCGTCA
GAAATGGCGTGGCGCGAAGATCACCGTCGTGTCAGCAATGGCGAACAGTACCTGATGATCGCGAGCGCGGCGCTCGCGCACCCCGTCAGCCGGCTGTGGA
AAGGCTATTGGCAGCGCCGCACGGGTTGACATTTGCCCGGGGGGATTCCCAGGCGAATCACGCCGTGTTACCTTCAGCTAAATGAAGCGATTTGTACCCA
ATATTCCGGAATGGTTTGAACCGCGCCGAGCGGCGCAGGTGACGGCGTTTTTTGCGCTTAAATCCGGTGGCAAAATCAACATTCTTAAGGCGACCAAGCT
CGTCTACCTGTCTGACCGGCTCAGTATGTCCCGACGCGATCACCCGATTACGGGGGACAATTTCGTTTCGATGCCGTTTGGACCCGTCAATACGTTTACT
TACAGCTACATGGACGGGGCAGCTCACTCCGACGCTTGGACGGAATTCGTAGCGCCGCGAAACGGCAACGAGCTTGAGCTGACCAAGAGGATTGATATTG
GCGATCTTGACGAGCTGAGCCGGTCAGACCTCAAAATTCTTGACGATACGTGGGAGGAATTCAAAGAGGTAGATCGCTTTGAATTAGCCGAGTGGACGCA
TAGATATTGCCCTGAGTGGAGAGACCCTGGCGGCTCCTCTATACCAATCGATTTTTCGACGGTTTTCAAAAACTTGAGCAAAGAATCGCCTGCTGAACTG
GCAGATGACATCCAAGCCGAGAGAGAACTGTTTATTCATCTCGCCGGTAAGTGATGTCATATAAGCCGTATCAAGGCGCAACGCTGCTGATACCTTATAA
CAACGTCCCTCATCTATTTTTCGTTCTCAACGAGCCATGCAAGGATGGCTTCTGCCTCCTAGTAATGGTGACATCAATAAATCCTAAGAAGTTACACGAC
GGCGCATGCGTTTTGCAGGCTGGTGATCACCCATTTGTCGTTCATCCAAGCTACTTGCTGTATCGTCTTGCGACTCAATCGCCGGCGCATCACATTCAGA
AGATGGTCGATAAGAAGTATTATGTTCCTAAAGAAGACTTGAGCAAAGCTGTCCTTGAGAGGGTCATTAATGGACTATGGTCTTCCGATGATACAAGGCC
TTCGATGCTTAGGTATGCAAAGGGCATTGGCCTAGAGCTTGATCCCAGTCCATAACCGTACGCCATCGCGCTTACCCTCTGAGACGATCAGCCCGCGCTT
AAGCCGCATGCTCAACGTCTGCACGACGCGATGCGCGACGGTACTGCGAAGCACCTTGTCGCTTTCGTCCATGCCCTTCGCGCGCATCACTCGCAGCGCC
AACTCGCGCGTGTCCAGAGGCGATTCGATCGCCAGCGCCTCATGGCAGAGCTTCCCGATCTCGCGGGGCCTGAACAGCCTTCCTATGTCCATGTAGACCG
GGAATTGAAGCGGCTCGTCTCCCACCTCGAATAGCCGGATCGTCGCGGCGATATGGGCCAGGTCGCGGCGTGCTGTCTCTAGCTTGTCCTCATACGCCTT
GATCGCGTCGTTGATGGCGTCGCGGCGGCGGCGAAGGGTGAGGAGAACGTTGGGGTCCGACATCAGAAAACACTAGACCAACGGAAAACGTTGGCATACG
TTCTCTTAGGTGAGCTTGCTACCCAATGCCG

Protein section

ORF number : 4

ORF 1

Length		Begin	End	Strand	Fusion ORF
603 bp	200 aa	146	748	+	No

ORF function : Passenger Gene
Annotation : Hypothetical proteinDescription :

ORF sequence :

MTTLQEFLHGRMNELATDDASLRRKLALNEQEREQLQKAAKAAGLHLKPITEAPPPPPATYVLSVGTSQGRATTQGVGMTVSRRPIPEKTIKEAVLEVLG
VLGTGLTALDLLSAINAKFDTDYPRTSLSPQLSRLKAEGKITRLGNLWSLAPDAPETNEPAHPTSEGTNERAQVPETNSTTVEPVGEVAHEKTLTVDPLE

Blast result :

ORF 2

Length		Begin	End	Strand	Fusion ORF
963 bp	320 aa	1267	2229	+	No

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MAQHFLLSAAARTLSLVTVARMSDDEAHSAFRQIRWCDTGGEPVCPRCNCGAVYTFKTRKLYKCKACTHQFSVTSGTIFASRKLAIRDYLLAIAIFVNGA
KGHSALQLSRDINVQYKTAFVLTHKLREAMSAEMADMTVSGEVEIDGAYFGGHVRPANFKENRVDRRLAKNQNGKRRVVVIMRERAGRTLPFVFKSEGAS
LATIGRRVHPSATVHADEALHWDELHTFYLTKRINHSEAYSDGQSCTNMAESFFSRLRRAEIGTHHHIAGPYLNAYSSEMAWREDHRRVSNGEQYLMIAS
AALAHPVSRLWKGYWQRRTG

Blast result :

ORF 3

Length		Begin	End	Strand	Fusion ORF
516 bp	171 aa	2339	2854	+	No

ORF function : Passenger Gene
Annotation : Hypothetical proteinDescription :

ORF sequence :

MTAFFALKSGGKINILKATKLVYLSDRLSMSRRDHPITGDNFVSMPFGPVNTFTYSYMDGAAHSDAWTEFVAPRNGNELELTKRIDIGDLDELSRSDLKI
LDDTWEEFKEVDRFELAEWTHRYCPEWRDPGGSSIPIDFSTVFKNLSKESPAELADDIQAERELFIHLAGK

Blast result :

ORF 4

Length		Begin	End	Strand	Fusion ORF
432 bp	143 aa	3663	3232	-	No

ORF function : Passenger Gene
Annotation : Hypothetical proteinDescription :

ORF sequence :

MSDPNVLLTLRRRRDAINDAIKAYEDKLETARRDLAHIAATIRLFEVGDEPLQFPVYMDIGRLFRPREIGKLCHEALAIESPLDTRELALRVMRAKGMDE
SDKVLRSTVAHRVVQTLSMRLKRGLIVSEGKRDGVRLWTGIKL

Blast result :

Comments

ISBos1 is 84% (transposase) aa similar to ISNwi4.

References

1] ISfinder annotation (2017)
2] Park,H (2016) Direct GenBank submission.