ISBp1

  • Family IS3
  • Group IS407
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
AF287634 ND Burkholderia pseudomallei
Burkholderia pseudomallei
DNA section
IS Length : 1214 bp

Ends


IR Length : 7/9

IRL : TGGAATTTTCGGACCAGTTGAAACTTGAGAGAATGGCTTCCAACGGAGAA
IRR : TGGGTTTTTGACCCGCTGACCTGCCTGATTTCTTCGGGCCATTTCAATCT

Insertion site


Left flankDirect repeatRight flankDR Length

DNA sequence

TGGAATTTTCGGACCAGTTGAAACTTGAGAGAATGGCTTCCAACGGAGAAAAGGAAGTCATGAAGAAGAGCCGGTTTACCGAAGAACAGATGGTCACCAT
CCTGCGCGAGGCGGACAAGGCGCCGGTCGCGGAAGTTGCGAAGAAGCACGGGGTCAGCGAGCAAACCATTTACAACTGGCGACAACATTTCGGCGGGCTG
GAGGCTGCCGACGTGAAGCGGCTCAAGCAGCTCGAGCAAGAGAATGCGCGGCTGAAGAAGATGCTGGCCGAGCGCGATCTCGAGCTGGACGTGATGAAAG
AGATCAACGCAAAAAAGTGGTGAGCGCGCCCGCCCGACGCCAGCAGGTCACCTATGCGAAAGCACGGGGCCTGTCGGAGCGACGTGCGTGCGCGCTGATG
TCGGTCGCCCGATCGGCGCTGCATTACGAATCAACGCTGGCCGTGCGGGACGCGCCGGTGCTGGCAGCGATGAGCATTCTGTCGGCGCAATATCCGCGTT
ATGGTTACCGCCGCATCCAGATCTTTCTGGAGCGGCAAGGTCACCCGATGAATGACGACCGGGCTTGGCGGCTGTGGCGTCTTGCCGGGTTGCAGGTGCC
GCGCAAGCGACCCCGTCGACGCGTGTCGGTCCAACGCCCTCGGCCGCAGCCGGCGACGGCGGCACGACACGTTTGGGCGTACGACTTCGTATTCGACGCG
TGCGCCAACGGCCAGCAGTTGAAGTGTCTGACGGTGATCGATGAGTACACGCGTGAATGCCTGGCAATCGACGTGGCCGGATCGATTCGTTCCGGACGTG
TCATCGAAGTCCTGTCCCAACTGGTCAGCCTTCATGGTGCACCTCGTTACTTGCGTTCGGACAACGGCCCAGAGTTCGTATCGCGGGCCACCCTGAAGTG
GGCTGCACAGAACGGCATGGACATGGCGCTTAGCGATCCCGGCAAGCCTTGGCAGAACGGCGCGGACGAGAGCTTCAACGGCAAGTTCCGAGATGAATGC
TTGAGCCTTGAATGGTTTCGAACGCGCACGGAAGCGAAGGTTGTGATCGAGCAGTGGCGGCGTCACTACAATGCCATCCGACCGCATTCGAGTCTGGCCT
ATCTGACGCCCGACGAGTTCAAGCAGCGGTATTGTTCAACTGAAGCAACCGAGGCCGTTCTCCAAGATTGAAATGGCCCGAAGAAATCAGGCAGGTCAGC
GGGTCAAAAACCCA
Recoding section
  • Recoding by frameshift
  • Frame
  • Type
  • Experimentally demonstrated

Stimulators :

  • Shine-Dalgarno sequence :
  • Secondary structure :

Recoding motif :

Protein section
ORF number : 3

 

ORF 1
LengthBeginEndStrandFusion ORF
264 bp87 aa60323+No
ORF function : Transposase
Description : First part of the transposase

ORF sequence :

MKKSRFTEEQMVTILREADKAPVAEVAKKHGVSEQTIYNWRQHFGGLEAADVKRLKQLEQENARLKKMLAERDLELDVMKEINAKKW

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
1143 bp380 aa291171+No
ORF function : Transposase
Description : Second part of the transposase

ORF sequence :

ENGFQRRKGSHEEEPVYRRTDGHHPARGGQGAGRGSCEEARGQRANHLQLATTFRRAGGCRREAAQAARARECAAEEDAGRARSRAGRDERDQRKKVVSA
PARRQQVTYAKARGLSERRACALMSVARSALHYESTLAVRDAPVLAAMSILSAQYPRYGYRRIQIFLERQGHPMNDDRAWRLWRLAGLQVPRKRPRRRVS
VQRPRPQPATAARHVWAYDFVFDACANGQQLKCLTVIDEYTRECLAIDVAGSIRSGRVIEVLSQLVSLHGAPRYLRSDNGPEFVSRATLKWAAQNGMDMA
LSDPGKPWQNGADESFNGKFRDECLSLEWFRTRTEAKVVIEQWRRHYNAIRPHSSLAYLTPDEFKQRYCSTEATEAVLQD

 

Blast result :
ORF 3
LengthBeginEndStrandFusion ORF
1111 bp370 aa601170+Yes
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MKKSRFTEEQMVTILREADKAPVAEVAKKHGVSEQTIYNWRQHFGGLEAADVKRLKQLEQENARLKKMLAERDLELDVMKEINAKKVVSAPARRQQVTYA
KARGLSERRACALMSVARSALHYESTLAVRDAPVLAAMSILSAQYPRYGYRRIQIFLERQGHPMNDDRAWRLWRLAGLQVPRKRPRRRVSVQRPRPQPAT
AARHVWAYDFVFDACANGQQLKCLTVIDEYTRECLAIDVAGSIRSGRVIEVLSQLVSLHGAPRYLRSDNGPEFVSRATLKWAAQNGMDMALSDPGKPWQN
GADESFNGKFRDECLSLEWFRTRTEAKVVIEQWRRHYNAIRPHSSLAYLTPDEFKQRYCSTEATEAVLQD

 

Blast result :
Comments
ISBp1 is 76% (ORF1) aa similar to ISD1 (ORF1)and 59% (ORF2) to ISRso14 (ORF2).
References
1] Yuen,K.Y., Woo,P.C.Y. and Leung,P.K.L (2000) Direct submission GenBank