ISBm3
- Family IS481
- Group
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| AF047478 | ND | Brucella melitensis | Brucella melitensis 16M |
DNA section
IS Length : 1022 bp
Ends
IR Length : 18/21
IRL : TGTGAGCTTTCAGGAGGTTGTCCATTCGGAGCGGATCGAGGAGACTGGAG
IRR : TGTGGAGCGTCAACAGGTTGTCTCGGTTGAGGCCGAGGCGACTGATTGGT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| AGGTAAAGGC | TCTAGC | AGGAGAATGA | 6 |
DNA sequence
TGTGAGCTTTCAGGAGGTTGTCCATTCGGAGCGGATCGAGGAGACTGGAGTTACCACACCTCAGTTTTCCAGGATTGCTCCGAATGAACATCCACAAGAA
TGCCCGACTGACGCCATTGCGTCGAGAAGAAATGGCGGTCGCAGTTTTCGGCGGCAGGCTGACGAAGGCGCAGGCTGCACGCCTTTATGGGGTCTCGCTG
AAAATTGTTTCTCGTTGGACCGAACGGTTCAGGATATCTGGCCGGGCGGCGATGACGGATCGCTCGTCGCGCCCCACCCGCAGCCCCCGTCAAATGGGCC
AGGACATTGTCGAACGGATACTCCATCTTCGCAGACAACGCCTGACCGGTAAGCATATCGCCATGGAAACCGGCGTTTCTCCCGCCACCGTCAGCCGCAT
TCTCAGGCGCGCAAAGCTGTCGCGGATGAAAGACATCGACCCGGTCGAACCCGTCATCCGCTACGAATATGCCGAGCCGGGAGGCCTGATCCATCTCGAC
ATCAAGCGGCTTGGGCGTTTCGAGCGCGTCGGCCATCGGATCACAGGCAACAGGACGAGACAAAGCAACACGCGCGGGGTTGGCTGGGAATATGTCCATG
TCTGCATCGATGATGTTTCCCGCATCGCCTTCACTGACATATTCCCGGATGAAAAAGCCATCATGCCATAGCCGTTCTCAAAGCTGCCGTCGCCTACTAT
GCAAGCCTTGGCGTCACCGTCACGCGGGTGATGACGGACAACGGGGCCTGCTATATCGCCAAGGCCTGCAAGGCGCTCGATCTCAAGCACATTCGAACCA
AGCCCTATACGCCAAAAACCAACGGCAAGGCCGAGCGCTTCATTCAGACCGCATTACGGGAGTGGGCCTATGCACGTGCATACCCATCGTCACAGATCCG
CAAAGCGCATCTGCCAAACTGGACACATATGTACAATTGGCATCGACCTCATAGCGGGCTAAAATCAAAAACACCAATCAGTCGCCTCGGCCTCAACCGA
GACAACCTGTTGACGCTCCACA
TGCCCGACTGACGCCATTGCGTCGAGAAGAAATGGCGGTCGCAGTTTTCGGCGGCAGGCTGACGAAGGCGCAGGCTGCACGCCTTTATGGGGTCTCGCTG
AAAATTGTTTCTCGTTGGACCGAACGGTTCAGGATATCTGGCCGGGCGGCGATGACGGATCGCTCGTCGCGCCCCACCCGCAGCCCCCGTCAAATGGGCC
AGGACATTGTCGAACGGATACTCCATCTTCGCAGACAACGCCTGACCGGTAAGCATATCGCCATGGAAACCGGCGTTTCTCCCGCCACCGTCAGCCGCAT
TCTCAGGCGCGCAAAGCTGTCGCGGATGAAAGACATCGACCCGGTCGAACCCGTCATCCGCTACGAATATGCCGAGCCGGGAGGCCTGATCCATCTCGAC
ATCAAGCGGCTTGGGCGTTTCGAGCGCGTCGGCCATCGGATCACAGGCAACAGGACGAGACAAAGCAACACGCGCGGGGTTGGCTGGGAATATGTCCATG
TCTGCATCGATGATGTTTCCCGCATCGCCTTCACTGACATATTCCCGGATGAAAAAGCCATCATGCCATAGCCGTTCTCAAAGCTGCCGTCGCCTACTAT
GCAAGCCTTGGCGTCACCGTCACGCGGGTGATGACGGACAACGGGGCCTGCTATATCGCCAAGGCCTGCAAGGCGCTCGATCTCAAGCACATTCGAACCA
AGCCCTATACGCCAAAAACCAACGGCAAGGCCGAGCGCTTCATTCAGACCGCATTACGGGAGTGGGCCTATGCACGTGCATACCCATCGTCACAGATCCG
CAAAGCGCATCTGCCAAACTGGACACATATGTACAATTGGCATCGACCTCATAGCGGGCTAAAATCAAAAACACCAATCAGTCGCCTCGGCCTCAACCGA
GACAACCTGTTGACGCTCCACA
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 939 bp | 313 aa | 84 | 1022 | + | No |
Chemistry : DDE
ORF sequence :
MNIHKNARLTPLRREEMAVAVFGGRLTKAQAARLYGVSLKIVSRWTERFRISGRAAMTDRSSRPTRSPRQMGQDIVERILHLRRQRLTGKHIAMETGVSP
ATVSRILRRAKLSRMKDIDPVEPVIRYEYAEPGGLIHLDIKRLGRFERVGHRITGNRTRQSNTRGVGWEYVHVCIDDVSRIAFTDIFPDEKAIMPIAVLK
AAVAYYASLGVTVTRVMTDNGACYIAKACKALDLKHIRTKPYTPKTNGKAERFIQTALREWAYARAYPSSQIRKAHLPNWTHMYNWHRPHSGLKSKTPIS
RLGLNRDNLLTLH
ATVSRILRRAKLSRMKDIDPVEPVIRYEYAEPGGLIHLDIKRLGRFERVGHRITGNRTRQSNTRGVGWEYVHVCIDDVSRIAFTDIFPDEKAIMPIAVLK
AAVAYYASLGVTVTRVMTDNGACYIAKACKALDLKHIRTKPYTPKTNGKAERFIQTALREWAYARAYPSSQIRKAHLPNWTHMYNWHRPHSGLKSKTPIS
RLGLNRDNLLTLH
Blast result :
Comments
There is a -1 frameshift at position 668. The last amino acid and the codon stop were in the insertion site.
The transposase is 55% aa similar to IS5564, IS1652, IS1649 and ISSco1, 54% to IS1122, 49% to IS1122 and IS1121 and 50% to ISCgl1.
The transposase is 55% aa similar to IS5564, IS1652, IS1649 and ISSco1, 54% to IS1122, 49% to IS1122 and IS1121 and 50% to ISCgl1.
References
1] Godfroid,F., Taminiau,B., Danese,I., Denoel,P., Tibor,A., Weynants,V., Cloeckaert,A., Godfroid,J. and Letesson,J.-J. (1998) Infect. Immun. 66 (11)