TnBth1
- Family Tn3
- Group
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| CP009350 | ND | Bacillus thuringiensis | Bacillus thuringiensis HD-789 pBTHD789-3 Bacillus thuringiensis HD1002 plasmid 4 Bacillus thuringiensis serovar israelensis AM65-52 pAM65-52-5-100K Bacillus thuringiensis Bt18247 p113275 |
DNA section
IS Length : 3672 bp
Ends
IR Length : 49/57
IRL : GGCCTTTGTGTAGCAATGGAACAGGATAACGCAACAAGCATTAGCGGACA
IRR : GGGGTATGTGTAGCAATGGAACAAAATCACGCAACAAGCATTAGCGGACA
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| GAAACAAAAAAATCGAATAT | AACAGTTACGAGTTTACTTT | 0 |
DNA sequence
GGCCTTTGTGTAGCAATGGAACAGGATAACGCAACAAGCATTAGCGGACATCAGAAATGATAAAAATGCACTTTTTATAAGAAAATCGTTTAGATTTAGA
TACATTTTCATTCTTATGAAACAGAAAAGTTCAGTTTTGTAAAAAGATCAAAAGCATATTTTACTGGTAATGTCCTATAAGTATCATTAGCGGACATTAA
GTCATTACAAATAGCTATTTTATGTCCACTAATATACGAAATAATCCTTAAAATGAATGTCCGCTAATACTTATTGCGTGATTTGGTTCCATTGCTACAC
ATACCCCATATTCTTCAGAAGTATATTAGACTTGCAAAGTTAGAAGGATTTAGTGCCCATGATTTAAGGCATCGCTTTGGTTATGTGATGGCTGAACGCA
CACCATTACATCGTCTTGCACAAATTATGGGACACGATAACTTGAATACCACGATGATTTATGTAAGAGCTACACAAGAAGATTTACAGGGAGAAGTAGA
AAAGATTGCCTGGAACTAAAGAATGCACATCATCCTACTCATTTGGTCATGTGATACAAACTAAGAATTGTAACAGGAGGAACAAGGGTTATGCCTGTAG
ATTTTTTAACACCTGAACAAGAAGAAAAATATGGTTGTTTTTGTGACACTCCAACATCAGAGCAGTTAGCAAAATATTTTTGGTTAGATGATACAGACAA
AGAACTGATATGGAATCGTCGTGGAGAGCATAATCAACTTGGTTTCGCTGTTCAATTAGGAACCGTTAGGTTCTTAGGAACATTTTTATCTGATCCTACA
AATGTACCACAATCGGTTATTACATATATGGCAAATCAACTTCATCTAGATGCTCAAAGCTTTTCTCGTTATCGAAATAAACGAAGTCAGTGGGATCAAA
TGCAAGAGATACGTTCTGTTTATGGATATAAAAACTTTACAGATAAATCAACACATTGGCGATTCATCAGATGGCTATATGCACGTGCTTGGCTATATAA
TGAACGACCAAGTGTCTTATTTGATTTAGCAACAGCACGATGTATCGAACAAAAAATTTTACTACCTGGTGTATCTGTATTAACAAGGCTAGTATCAACG
GTTCGTGATCGTTCAGCAGAAAATATATGGAAAAAGCTCTCTAGTCTTCCGGATAATGTTCAGAAAAAACAATTAGAAAATCTTCTTCAGATAGATCAAA
AAACAAAGAAAACGTATTTAGAGCGTCTAAGTAATCCCCCTGTTACGATTAGTGTTACGGGCATTAAGAATACGCTGATTCGTTTACAAGAGCTTCGTCA
ATTGAACACTGAAAATTGGGATATGTCTAGAATTCCTTCGAAAAGATTACAACAATTCGCTCGTCACACAGTCGCTGTTAGAGCACAAGCAATTGCTAGA
ATGCCCGATCAACGACGTATGGCTATGTTAGTTGCATTTGCTAAAATGTATACACAAAGTGCTCAGGATGATGTCATTGATATTTTTGATAGATATTTAA
CAGATTTATTTGCTAAGACATATCGAAAGGAACAAAAAGAACGTCTTCGTACAATTAAGGATTTAGATAAGGCAGCGCGCCAATTACGGGAAGCTTGTGT
AATATTATTAGAACATACGGATCCTTCTATCCATCCAAAAACGGCAGTGTTTGAAAAAATCTCAGAAAAGGATTTGATACAAGCTGTCCAAATTGTTGAT
TCACTCACCTATTCACCAAATCAAACACTAGCCTATTCAGGATTGTTACAACATTATGGCACAATCCGAAAATTTCTTCCTTTACTCATGGAAGAAATTG
AATTACAAGCAACGCCTGCTGGATTACCCATCTTGCAAGCATGGAATTTTGTAAAAGAGCATGGGAAATCCAATAAGAAAAGATGGAAAAATGCTCCTCT
TGCCGGTTTGAATGCAAATTGGTCTAAGGTTGTAATTGATAAAGATTCCGGAACTGTAAATCATCGAGCATATACGTTTTGGATGCTCGAACAAGTATTA
GAAGCTTTGCACCGACACGATCTATATATAGTAGGAAGTGAAAAATATGGGGACCTTCGCGCACAATTATTACAAGGCGAAGAATGGAAAAGTATTCGCC
CTAGTATTCTTCGCTCATTAGACTGGTCAATAGATTCTTATGAATCATTGACACCGTTAAAAGAAGAGTTAGAGAAAACTTATCATCAAGTCATTGAGAA
TTGGGAGAATAATCCTGCGGTGCAAATAGACACATTTGCAGGTAAAGAGAGAATTGTTTTGACACCTTTAGACAAACAACCAGAACCTGAATCACTACAA
AAACTAAAACAACAAATACATACGATGTTGCCAAATATAGATATTCCTCAATTATTACTCGAAGTAAATCGTTGGACGGGATTTATGGATGGTTTTCGAC
ATATTAGTGAGGCTAAATCCAGAATTAACGAGTTACCTATAAGTATCTGTGCATTGCTTATATCTCAAGCATGCAATATCGGGTTAAGACCTTTAGTTCA
AGATGGGGTTCCTTCATTAGAACGTGATCGTCTTACATGGATTGAACAAAATTATTTTCGTGCAGAAACACTTTCAGAATCAAACGCGAAACTTGTAGAT
TTTCATAGTCAATTACAGCTGGCTAAAATGTGGGGTGGTGGAGAAATTGCTTCAGCTGATGGATTACGTTTCATCACACCAGTAAAATCCGTACACACTG
GTCCAAATCCTAAATATTTCGGTTCTGGTCGTGGTGTTACGTATTACAACTATACGAGCGATCAATTTACCGGACTCCACGGTTTGGTGATTCCAGGCAC
AATTCGTGATTCATTATACTTACTTCAATGTGTGTTAGAACAAAATACGAACTTACAGCCAAAAGAAATTATGACAGATACAGCTGGGTATAGTGATATT
ATTTTTGGGCTCTTTGGATTATTAGGATATCAATTTAGTCCTCGTTTAGCTGATATCAGTGAATCACGTCTTTGGCGTTTTGATGCGAACTCAGATTATA
GCATGTTAAATAATTTGTCTAAAAGTCGCATTCGTGAAGAACTCATACATCGTCATTGGGAAGACATGCTTCGTGTTGCGGGATCTTTGAAACTAAATAA
AATAAATGCAACACATCTTATCCAAGCACTTCAGTATAATGGGAAACCAACTATGTTAGGGCGAGCAATTGGAGAATTGGGGAGACTCTTTAAAACACGT
TATTTACTCTTATATTTACATGATGAAAATTATCGTCGTAAAATTTTAAATCAACTCAATAGAGGGGAAGCAAGGCATAGTTTAGCGAGGGCTGTATTTT
ACGGCAAACGTGGAGAACTTCATCAATCCTATCGAGAAGGACAAGAAGAGCAATTAGGCGCATTAGGTTTAGTAGTAAATGCAATTATTGTATGGAATAC
ACGATATATAGAATCTGCATTACAAGTACTCCGAAATCGCGGTCATACAATTGATAATGATGATATATCTAGACTTTCACCATTAGGCCATAAACACATT
AACATAGTAGGTCGGTATTCATTTGTTCTCCCAGAAGAAGTAAAAGATGGGCAATTGCGTACACTAACATATGAAGAAACAAACAAAAAGGAACCTGATT
CTTTATAAGAATAGGTTCCTAATGTCCGCTAATGCTTGTTGCGTGATTTTGTTCCATTGCTACACATACCCC
TACATTTTCATTCTTATGAAACAGAAAAGTTCAGTTTTGTAAAAAGATCAAAAGCATATTTTACTGGTAATGTCCTATAAGTATCATTAGCGGACATTAA
GTCATTACAAATAGCTATTTTATGTCCACTAATATACGAAATAATCCTTAAAATGAATGTCCGCTAATACTTATTGCGTGATTTGGTTCCATTGCTACAC
ATACCCCATATTCTTCAGAAGTATATTAGACTTGCAAAGTTAGAAGGATTTAGTGCCCATGATTTAAGGCATCGCTTTGGTTATGTGATGGCTGAACGCA
CACCATTACATCGTCTTGCACAAATTATGGGACACGATAACTTGAATACCACGATGATTTATGTAAGAGCTACACAAGAAGATTTACAGGGAGAAGTAGA
AAAGATTGCCTGGAACTAAAGAATGCACATCATCCTACTCATTTGGTCATGTGATACAAACTAAGAATTGTAACAGGAGGAACAAGGGTTATGCCTGTAG
ATTTTTTAACACCTGAACAAGAAGAAAAATATGGTTGTTTTTGTGACACTCCAACATCAGAGCAGTTAGCAAAATATTTTTGGTTAGATGATACAGACAA
AGAACTGATATGGAATCGTCGTGGAGAGCATAATCAACTTGGTTTCGCTGTTCAATTAGGAACCGTTAGGTTCTTAGGAACATTTTTATCTGATCCTACA
AATGTACCACAATCGGTTATTACATATATGGCAAATCAACTTCATCTAGATGCTCAAAGCTTTTCTCGTTATCGAAATAAACGAAGTCAGTGGGATCAAA
TGCAAGAGATACGTTCTGTTTATGGATATAAAAACTTTACAGATAAATCAACACATTGGCGATTCATCAGATGGCTATATGCACGTGCTTGGCTATATAA
TGAACGACCAAGTGTCTTATTTGATTTAGCAACAGCACGATGTATCGAACAAAAAATTTTACTACCTGGTGTATCTGTATTAACAAGGCTAGTATCAACG
GTTCGTGATCGTTCAGCAGAAAATATATGGAAAAAGCTCTCTAGTCTTCCGGATAATGTTCAGAAAAAACAATTAGAAAATCTTCTTCAGATAGATCAAA
AAACAAAGAAAACGTATTTAGAGCGTCTAAGTAATCCCCCTGTTACGATTAGTGTTACGGGCATTAAGAATACGCTGATTCGTTTACAAGAGCTTCGTCA
ATTGAACACTGAAAATTGGGATATGTCTAGAATTCCTTCGAAAAGATTACAACAATTCGCTCGTCACACAGTCGCTGTTAGAGCACAAGCAATTGCTAGA
ATGCCCGATCAACGACGTATGGCTATGTTAGTTGCATTTGCTAAAATGTATACACAAAGTGCTCAGGATGATGTCATTGATATTTTTGATAGATATTTAA
CAGATTTATTTGCTAAGACATATCGAAAGGAACAAAAAGAACGTCTTCGTACAATTAAGGATTTAGATAAGGCAGCGCGCCAATTACGGGAAGCTTGTGT
AATATTATTAGAACATACGGATCCTTCTATCCATCCAAAAACGGCAGTGTTTGAAAAAATCTCAGAAAAGGATTTGATACAAGCTGTCCAAATTGTTGAT
TCACTCACCTATTCACCAAATCAAACACTAGCCTATTCAGGATTGTTACAACATTATGGCACAATCCGAAAATTTCTTCCTTTACTCATGGAAGAAATTG
AATTACAAGCAACGCCTGCTGGATTACCCATCTTGCAAGCATGGAATTTTGTAAAAGAGCATGGGAAATCCAATAAGAAAAGATGGAAAAATGCTCCTCT
TGCCGGTTTGAATGCAAATTGGTCTAAGGTTGTAATTGATAAAGATTCCGGAACTGTAAATCATCGAGCATATACGTTTTGGATGCTCGAACAAGTATTA
GAAGCTTTGCACCGACACGATCTATATATAGTAGGAAGTGAAAAATATGGGGACCTTCGCGCACAATTATTACAAGGCGAAGAATGGAAAAGTATTCGCC
CTAGTATTCTTCGCTCATTAGACTGGTCAATAGATTCTTATGAATCATTGACACCGTTAAAAGAAGAGTTAGAGAAAACTTATCATCAAGTCATTGAGAA
TTGGGAGAATAATCCTGCGGTGCAAATAGACACATTTGCAGGTAAAGAGAGAATTGTTTTGACACCTTTAGACAAACAACCAGAACCTGAATCACTACAA
AAACTAAAACAACAAATACATACGATGTTGCCAAATATAGATATTCCTCAATTATTACTCGAAGTAAATCGTTGGACGGGATTTATGGATGGTTTTCGAC
ATATTAGTGAGGCTAAATCCAGAATTAACGAGTTACCTATAAGTATCTGTGCATTGCTTATATCTCAAGCATGCAATATCGGGTTAAGACCTTTAGTTCA
AGATGGGGTTCCTTCATTAGAACGTGATCGTCTTACATGGATTGAACAAAATTATTTTCGTGCAGAAACACTTTCAGAATCAAACGCGAAACTTGTAGAT
TTTCATAGTCAATTACAGCTGGCTAAAATGTGGGGTGGTGGAGAAATTGCTTCAGCTGATGGATTACGTTTCATCACACCAGTAAAATCCGTACACACTG
GTCCAAATCCTAAATATTTCGGTTCTGGTCGTGGTGTTACGTATTACAACTATACGAGCGATCAATTTACCGGACTCCACGGTTTGGTGATTCCAGGCAC
AATTCGTGATTCATTATACTTACTTCAATGTGTGTTAGAACAAAATACGAACTTACAGCCAAAAGAAATTATGACAGATACAGCTGGGTATAGTGATATT
ATTTTTGGGCTCTTTGGATTATTAGGATATCAATTTAGTCCTCGTTTAGCTGATATCAGTGAATCACGTCTTTGGCGTTTTGATGCGAACTCAGATTATA
GCATGTTAAATAATTTGTCTAAAAGTCGCATTCGTGAAGAACTCATACATCGTCATTGGGAAGACATGCTTCGTGTTGCGGGATCTTTGAAACTAAATAA
AATAAATGCAACACATCTTATCCAAGCACTTCAGTATAATGGGAAACCAACTATGTTAGGGCGAGCAATTGGAGAATTGGGGAGACTCTTTAAAACACGT
TATTTACTCTTATATTTACATGATGAAAATTATCGTCGTAAAATTTTAAATCAACTCAATAGAGGGGAAGCAAGGCATAGTTTAGCGAGGGCTGTATTTT
ACGGCAAACGTGGAGAACTTCATCAATCCTATCGAGAAGGACAAGAAGAGCAATTAGGCGCATTAGGTTTAGTAGTAAATGCAATTATTGTATGGAATAC
ACGATATATAGAATCTGCATTACAAGTACTCCGAAATCGCGGTCATACAATTGATAATGATGATATATCTAGACTTTCACCATTAGGCCATAAACACATT
AACATAGTAGGTCGGTATTCATTTGTTCTCCCAGAAGAAGTAAAAGATGGGCAATTGCGTACACTAACATATGAAGAAACAAACAAAAAGGAACCTGATT
CTTTATAAGAATAGGTTCCTAATGTCCGCTAATGCTTGTTGCGTGATTTTGTTCCATTGCTACACATACCCC
Protein section
ORF number : 2
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 246 bp | 81 aa | 274 | 519 | + | No |
AG : Tn3 resolvase
ORF sequence :
MRDLVPLLHIPHILQKYIRLAKLEGFSAHDLRHRFGYVMAERTPLHRLAQIMGHDNLNTTMIYVRATQEDLQGEVEKIAWN
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 3018 bp | 1005 aa | 591 | 3608 | + | No |
Chemistry : DDE
ORF sequence :
MPVDFLTPEQEEKYGCFCDTPTSEQLAKYFWLDDTDKELIWNRRGEHNQLGFAVQLGTVRFLGTFLSDPTNVPQSVITYMANQLHLDAQSFSRYRNKRSQ
WDQMQEIRSVYGYKNFTDKSTHWRFIRWLYARAWLYNERPSVLFDLATARCIEQKILLPGVSVLTRLVSTVRDRSAENIWKKLSSLPDNVQKKQLENLLQ
IDQKTKKTYLERLSNPPVTISVTGIKNTLIRLQELRQLNTENWDMSRIPSKRLQQFARHTVAVRAQAIARMPDQRRMAMLVAFAKMYTQSAQDDVIDIFD
RYLTDLFAKTYRKEQKERLRTIKDLDKAARQLREACVILLEHTDPSIHPKTAVFEKISEKDLIQAVQIVDSLTYSPNQTLAYSGLLQHYGTIRKFLPLLM
EEIELQATPAGLPILQAWNFVKEHGKSNKKRWKNAPLAGLNANWSKVVIDKDSGTVNHRAYTFWMLEQVLEALHRHDLYIVGSEKYGDLRAQLLQGEEWK
SIRPSILRSLDWSIDSYESLTPLKEELEKTYHQVIENWENNPAVQIDTFAGKERIVLTPLDKQPEPESLQKLKQQIHTMLPNIDIPQLLLEVNRWTGFMD
GFRHISEAKSRINELPISICALLISQACNIGLRPLVQDGVPSLERDRLTWIEQNYFRAETLSESNAKLVDFHSQLQLAKMWGGGEIASADGLRFITPVKS
VHTGPNPKYFGSGRGVTYYNYTSDQFTGLHGLVIPGTIRDSLYLLQCVLEQNTNLQPKEIMTDTAGYSDIIFGLFGLLGYQFSPRLADISESRLWRFDAN
SDYSMLNNLSKSRIREELIHRHWEDMLRVAGSLKLNKINATHLIQALQYNGKPTMLGRAIGELGRLFKTRYLLLYLHDENYRRKILNQLNRGEARHSLAR
AVFYGKRGELHQSYREGQEEQLGALGLVVNAIIVWNTRYIESALQVLRNRGHTIDNDDISRLSPLGHKHINIVGRYSFVLPEEVKDGQLRTLTYEETNKK
EPDSL
WDQMQEIRSVYGYKNFTDKSTHWRFIRWLYARAWLYNERPSVLFDLATARCIEQKILLPGVSVLTRLVSTVRDRSAENIWKKLSSLPDNVQKKQLENLLQ
IDQKTKKTYLERLSNPPVTISVTGIKNTLIRLQELRQLNTENWDMSRIPSKRLQQFARHTVAVRAQAIARMPDQRRMAMLVAFAKMYTQSAQDDVIDIFD
RYLTDLFAKTYRKEQKERLRTIKDLDKAARQLREACVILLEHTDPSIHPKTAVFEKISEKDLIQAVQIVDSLTYSPNQTLAYSGLLQHYGTIRKFLPLLM
EEIELQATPAGLPILQAWNFVKEHGKSNKKRWKNAPLAGLNANWSKVVIDKDSGTVNHRAYTFWMLEQVLEALHRHDLYIVGSEKYGDLRAQLLQGEEWK
SIRPSILRSLDWSIDSYESLTPLKEELEKTYHQVIENWENNPAVQIDTFAGKERIVLTPLDKQPEPESLQKLKQQIHTMLPNIDIPQLLLEVNRWTGFMD
GFRHISEAKSRINELPISICALLISQACNIGLRPLVQDGVPSLERDRLTWIEQNYFRAETLSESNAKLVDFHSQLQLAKMWGGGEIASADGLRFITPVKS
VHTGPNPKYFGSGRGVTYYNYTSDQFTGLHGLVIPGTIRDSLYLLQCVLEQNTNLQPKEIMTDTAGYSDIIFGLFGLLGYQFSPRLADISESRLWRFDAN
SDYSMLNNLSKSRIREELIHRHWEDMLRVAGSLKLNKINATHLIQALQYNGKPTMLGRAIGELGRLFKTRYLLLYLHDENYRRKILNQLNRGEARHSLAR
AVFYGKRGELHQSYREGQEEQLGALGLVVNAIIVWNTRYIESALQVLRNRGHTIDNDDISRLSPLGHKHINIVGRYSFVLPEEVKDGQLRTLTYEETNKK
EPDSL
Blast result :
Comments
TnBth1 is 66% (Transposase) aa similar to Tn3.
References
1] ISfinder annotation (2017)
2] Johnson,S.L., Daligault,H.E., Davenport,K.W., Jaissle,J., Frey,K.G., Ladner,J.T., Broomall,S.M., Bishop-Lilly,K.A., Bruce,D.C., Gibbons,H.S., Coyne,S.R., Lo,C.C., Meincke,L., Munk,A.C., Koroleva,G.I., Rosenzweig,C.N., Palacios,G.F., Redden,C.L., Minogue,T.D. and Chain,P.S. (2015) Genome Announc 3 (2).
2] Johnson,S.L., Daligault,H.E., Davenport,K.W., Jaissle,J., Frey,K.G., Ladner,J.T., Broomall,S.M., Bishop-Lilly,K.A., Bruce,D.C., Gibbons,H.S., Coyne,S.R., Lo,C.C., Meincke,L., Munk,A.C., Koroleva,G.I., Rosenzweig,C.N., Palacios,G.F., Redden,C.L., Minogue,T.D. and Chain,P.S. (2015) Genome Announc 3 (2).