ISPa74
- Family IS3
- Group IS3
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| ND | Pseudomonas aeruginosa | Pseudomonas aeruginosa |
DNA section
IS Length : 1654 bp
Ends
IR Length : 15
IRL : CCCCCTCTGGCAGGATAGTTGTCACCCTCTGATTTCAACCAGATAGGCGG
IRR : CGGTGTTTTTCAAGATAGTTGTCACTGTTTTCTTGCTAAGCCGCTTGTTT
Comments : caagatagttgtcac -- gtgacaactatcctg
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| GGTAC | 5 |
DNA sequence
CCCCCTCTGGCAGGATAGTTGTCACCCTCTGATTTCAACCAGATAGGCGGACAAGGATACATAGTGGCCCGTTATTCCGAAGAGCGTAAGGAAGCCGTAT
TGAGCAAGCTGCTACCTCCCTACAACATGACCGTGGCCGAGCTAGCCCGTCAGGAAGGCATCTCCGAGCCGACCCTTTATACTTGGCGTAACAAAGCCAA
GTCAGAAGGACGCCCCGTGCCAGGTCGCAAAGCCACATCAGAGCAATGGTCAGCTGAAGCCAAGCTCGCTACCGTCATCGAAACCGCAGCTCTTTCCGAG
GCCGAGCTCAGCCAGTATTGCCGGGAAAAGGGCCTTTATCCTGACCAGGTCAAGCAGTGGAAGGTGGAGTCCTTACAGGGCTTCCAGCGCAGTGCGGAGC
AGGATAAGGCCATACGCCAACAGGCCCGCAGCGACAAACAGGAAATAAAGCAGTTACGCCGAGAGTTGCGTCATAAGGAGAAAGCGCTGGCTGAAGCGGC
TGCCTTGTTGGTACTGCGAAAAAAGCTCAATGCGCTCTGGGACAACGACAGCAACGAGGACGAATGACCTCCATCCCGGAGCGCCAAGCCATCGTCAATC
TGATCCAGCAAGCGAGCTGTGATGGTGCCCGTCTGGCCAGGGCCTGTGCTGAAGCCGATATCTGTCTGCGTACCTACCGTCGCTGGTATCGCGACGGGAT
TGTGCAAGACGACAAGCGCCCCACAGCGGTCAGACCAGTCCCTGCCAACAAGCTGACAGCGACGGAGCAGGAGCAGATTGTCGAGCTGTGCAACAGCCCG
GCCTACAGCCAGCTGCCACCGAGCCAGATCGTGCCTGACCTGCTGGACAAGGGAGTTTATCTCGCATCGGAGTCCACCTTCTACCGTGTGCTCAAGGAGG
CAGGTCAGCTGCATCATCGGGGACAGTCACTGGCACCCAGGCTCAGTCCCGCACCAACGACTCATACAGCCAGCGGCCCCTGCGAAGTCTGGTGCTGGGA
CATTACCTACTGCCCTTCGACGGTGCGTGGGCAGTTCTACTACCTGTACATGTTCGAGGATCTCTACAGCCGCAAGATCGTCGGTTATGAAGTCCATGAG
ACCGAGAGTGGCGAGTATGCCGCCCAGCTCCTGCAACGTTGCCAGTTGCGTGAACAGTGTCTGCACAAGCCGCTGGTGCTGCACTCGGATAACGGTGCGC
CGATGAAGGCGCAGACCATGAAGGCCAAGCTGGAAGAACTGGGCGTGCTGCCCTCGTACAGCCGTCCCCGGGTCAGCAACGATAATGCCTTCGTTGAATC
GCTGTTCCGGGTACTGAAGTACCGGCCTCAGTGGCCCTCCCGTGGCTTTACCTCGCTGGAGGAAGCACGGCGGTGGGTAGACCGGTTCGTGCGCTGGTAC
AACACCGAGCATCGGCACAGCAAGCTGCGCTTCGTGACACCGGAGCAACGCCACAACGGCGAGGATGCGGTGCTGCTGGAACAGCGCAAGTGGGTATTGG
AGCAGGCCAAACAGAGTATGCCGTCACGCTGGGGTACGCGCCCCGTCAGAAACTGCGAGCCTGTTGGGCCGACGACATTGAACCCGGAAAAGGAACAGCT
TATGAAACAAGCGGCTTAGCAAGAAAACAGTGACAACTATCTTGAAAAACACCG
TGAGCAAGCTGCTACCTCCCTACAACATGACCGTGGCCGAGCTAGCCCGTCAGGAAGGCATCTCCGAGCCGACCCTTTATACTTGGCGTAACAAAGCCAA
GTCAGAAGGACGCCCCGTGCCAGGTCGCAAAGCCACATCAGAGCAATGGTCAGCTGAAGCCAAGCTCGCTACCGTCATCGAAACCGCAGCTCTTTCCGAG
GCCGAGCTCAGCCAGTATTGCCGGGAAAAGGGCCTTTATCCTGACCAGGTCAAGCAGTGGAAGGTGGAGTCCTTACAGGGCTTCCAGCGCAGTGCGGAGC
AGGATAAGGCCATACGCCAACAGGCCCGCAGCGACAAACAGGAAATAAAGCAGTTACGCCGAGAGTTGCGTCATAAGGAGAAAGCGCTGGCTGAAGCGGC
TGCCTTGTTGGTACTGCGAAAAAAGCTCAATGCGCTCTGGGACAACGACAGCAACGAGGACGAATGACCTCCATCCCGGAGCGCCAAGCCATCGTCAATC
TGATCCAGCAAGCGAGCTGTGATGGTGCCCGTCTGGCCAGGGCCTGTGCTGAAGCCGATATCTGTCTGCGTACCTACCGTCGCTGGTATCGCGACGGGAT
TGTGCAAGACGACAAGCGCCCCACAGCGGTCAGACCAGTCCCTGCCAACAAGCTGACAGCGACGGAGCAGGAGCAGATTGTCGAGCTGTGCAACAGCCCG
GCCTACAGCCAGCTGCCACCGAGCCAGATCGTGCCTGACCTGCTGGACAAGGGAGTTTATCTCGCATCGGAGTCCACCTTCTACCGTGTGCTCAAGGAGG
CAGGTCAGCTGCATCATCGGGGACAGTCACTGGCACCCAGGCTCAGTCCCGCACCAACGACTCATACAGCCAGCGGCCCCTGCGAAGTCTGGTGCTGGGA
CATTACCTACTGCCCTTCGACGGTGCGTGGGCAGTTCTACTACCTGTACATGTTCGAGGATCTCTACAGCCGCAAGATCGTCGGTTATGAAGTCCATGAG
ACCGAGAGTGGCGAGTATGCCGCCCAGCTCCTGCAACGTTGCCAGTTGCGTGAACAGTGTCTGCACAAGCCGCTGGTGCTGCACTCGGATAACGGTGCGC
CGATGAAGGCGCAGACCATGAAGGCCAAGCTGGAAGAACTGGGCGTGCTGCCCTCGTACAGCCGTCCCCGGGTCAGCAACGATAATGCCTTCGTTGAATC
GCTGTTCCGGGTACTGAAGTACCGGCCTCAGTGGCCCTCCCGTGGCTTTACCTCGCTGGAGGAAGCACGGCGGTGGGTAGACCGGTTCGTGCGCTGGTAC
AACACCGAGCATCGGCACAGCAAGCTGCGCTTCGTGACACCGGAGCAACGCCACAACGGCGAGGATGCGGTGCTGCTGGAACAGCGCAAGTGGGTATTGG
AGCAGGCCAAACAGAGTATGCCGTCACGCTGGGGTACGCGCCCCGTCAGAAACTGCGAGCCTGTTGGGCCGACGACATTGAACCCGGAAAAGGAACAGCT
TATGAAACAAGCGGCTTAGCAAGAAAACAGTGACAACTATCTTGAAAAACACCG
Recoding section
- Recoding by frameshift
- Frame -1
- Type translational
- Experimentally demonstrated No
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 504 bp | 167 aa | 64 | 567 | + | No |
Description : First part of the transposase
ORF sequence :
VARYSEERKEAVLSKLLPPYNMTVAELARQEGISEPTLYTWRNKAKSEGRPVPGRKATSEQWSAEAKLATVIETAALSEAELSQYCREKGLYPDQVKQWK
VESLQGFQRSAEQDKAIRQQARSDKQEIKQLRRELRHKEKALAEAAALLVLRKKLNALWDNDSNEDE
VESLQGFQRSAEQDKAIRQQARSDKQEIKQLRRELRHKEKALAEAAALLVLRKKLNALWDNDSNEDE
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1125 bp | 374 aa | 495 | 1619 | + | No |
Description : Second part of the transposase
ORF sequence :
SGCLVGTAKKAQCALGQRQQRGRMTSIPERQAIVNLIQQASCDGARLARACAEADICLRTYRRWYRDGIVQDDKRPTAVRPVPANKLTATEQEQIVELCN
SPAYSQLPPSQIVPDLLDKGVYLASESTFYRVLKEAGQLHHRGQSLAPRLSPAPTTHTASGPCEVWCWDITYCPSTVRGQFYYLYMFEDLYSRKIVGYEV
HETESGEYAAQLLQRCQLREQCLHKPLVLHSDNGAPMKAQTMKAKLEELGVLPSYSRPRVSNDNAFVESLFRVLKYRPQWPSRGFTSLEEARRWVDRFVR
WYNTEHRHSKLRFVTPEQRHNGEDAVLLEQRKWVLEQAKQSMPSRWGTRPVRNCEPVGPTTLNPEKEQLMKQAA
SPAYSQLPPSQIVPDLLDKGVYLASESTFYRVLKEAGQLHHRGQSLAPRLSPAPTTHTASGPCEVWCWDITYCPSTVRGQFYYLYMFEDLYSRKIVGYEV
HETESGEYAAQLLQRCQLREQCLHKPLVLHSDNGAPMKAQTMKAKLEELGVLPSYSRPRVSNDNAFVESLFRVLKYRPQWPSRGFTSLEEARRWVDRFVR
WYNTEHRHSKLRFVTPEQRHNGEDAVLLEQRKWVLEQAKQSMPSRWGTRPVRNCEPVGPTTLNPEKEQLMKQAA
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1556 bp | 518 aa | 64 | 1619 | + | Yes |
Chemistry : DDE
ORF sequence :
VARYSEERKEAVLSKLLPPYNMTVAELARQEGISEPTLYTWRNKAKSEGRPVPGRKATSEQWSAEAKLATVIETAALSEAELSQYCREKGLYPDQVKQWK
VESLQGFQRSAEQDKAIRQQARSDKQEIKQLRRELRHKEKALAEAAALLVLRKKAQCALGQRQQRGRMTSIPERQAIVNLIQQASCDGARLARACAEADI
CLRTYRRWYRDGIVQDDKRPTAVRPVPANKLTATEQEQIVELCNSPAYSQLPPSQIVPDLLDKGVYLASESTFYRVLKEAGQLHHRGQSLAPRLSPAPTT
HTASGPCEVWCWDITYCPSTVRGQFYYLYMFEDLYSRKIVGYEVHETESGEYAAQLLQRCQLREQCLHKPLVLHSDNGAPMKAQTMKAKLEELGVLPSYS
RPRVSNDNAFVESLFRVLKYRPQWPSRGFTSLEEARRWVDRFVRWYNTEHRHSKLRFVTPEQRHNGEDAVLLEQRKWVLEQAKQSMPSRWGTRPVRNCEP
VGPTTLNPEKEQLMKQAA
VESLQGFQRSAEQDKAIRQQARSDKQEIKQLRRELRHKEKALAEAAALLVLRKKAQCALGQRQQRGRMTSIPERQAIVNLIQQASCDGARLARACAEADI
CLRTYRRWYRDGIVQDDKRPTAVRPVPANKLTATEQEQIVELCNSPAYSQLPPSQIVPDLLDKGVYLASESTFYRVLKEAGQLHHRGQSLAPRLSPAPTT
HTASGPCEVWCWDITYCPSTVRGQFYYLYMFEDLYSRKIVGYEVHETESGEYAAQLLQRCQLREQCLHKPLVLHSDNGAPMKAQTMKAKLEELGVLPSYS
RPRVSNDNAFVESLFRVLKYRPQWPSRGFTSLEEARRWVDRFVRWYNTEHRHSKLRFVTPEQRHNGEDAVLLEQRKWVLEQAKQSMPSRWGTRPVRNCEP
VGPTTLNPEKEQLMKQAA
Blast result :
Comments
ISPa74 is 80% aa similar to ISSde10.
The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
The ISPa74 ends are not classic for this ISs family.
The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
The ISPa74 ends are not classic for this ISs family.
References
1] Petitjean Marie (2017) Direct submission to ISfinder