ISStin7

  • Family IS3
  • Group IS150
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
ND Streptococcus iniae
Streptococcus iniae 89353
Streptococcus iniae YSFST01-82
Streptococcus iniae ISNO
Streptococcus iniae ISET0901
Streptococcus iniae SF1
Streptococcus iniae
DNA section
IS Length : 1427 bp

Ends


IR Length : 20/25

IRL : TGAACTGCACCCCAAAAGTTAGACACAAAATCTAACAGTTGGGGTGTTTT
IRR : TGTACTGACCCCCAGAAGTTGGACAATTAATTATTGCAAGGATTTGGTTC

Insertion site


Left flankDirect repeatRight flankDR Length
AAAAGACTGCTCGTAAGGCTTTT3
TTTAGAAAAAATCTAAGGCTTTT3
AAAAAGACTAGATGTTCCAAAAC3
AAAGCCTATGAAATCAACGTTTC3
AAAGACTCGGGATTGCTTAGCAA3

DNA sequence

TGAACTGCACCCCAAAAGTTAGACACAAAATCTAACAGTTGGGGTGTTTTTCTTATGAAATTAAGTTATGAAGATAAACTAGAAATATATGAGCTGAAAA
AGAGCGGCGTGTCGTGGACCAATCTTAGCCAGACATACAAGGTAACCATTGCCAATCTCACATACATGATAAAACTCATGGATCGGTATGGCTTAGAAAT
CGTTGAAAAAGGTAAAAATAGGTATTATTCACTCGAATTAAAGCAGGAAATGATAGATAAAGTCTTGATTCATGGTTGCTCTCAACTCTCAGTTTCTCTT
GATTATACCTTGCCAAATCGAGGGATGCTCCCAAATTGGATAGCGCAATACAAGAAAAACGGTTATACTATTCTTGAAAAACCAAGAGGGAGACCTGCTA
AGATGGGACGAAAGCCTAAGAAAAAGCTAGAAGAGATGACTGAATTGGAACGTCTTCAGAAAGAATTAGAATACTTAAGAGCGGAGAATGCTGTGCTAAA
AAAGCTGAGAGAATACCGCTTGAGGGACGAAGCAAAGCTCAAAGAGCAACAGAAATCATTCAAGCATTAACGAATCGGTTTTCCCTAGAGACGCTACTTG
AAATCCTTGATTTACCGCGCTCCACCTATTACTATCAAGTGAAGCGACTAGCTCAAGGAGATAAGGATATAGAACTAAAGAATTTGATTCGAGAGATTTA
TGATGAACATAAAGGAAACTATGGCTATCGTCGGATTTATTTAGAACTCAGAAATCGAGGATTCCTCATCAATCACAAAAAGGTGCAACGCTTGATGACA
GTTATGGGCTTAGCGGCTCGCATTCGTCGTAAGCGCAAGTACTCTTCTTACAAAGGTGAGGTTGGTAAGAAGGCTGATAATCTGATTAAACGTCAATTCG
AAGGTTCTAAACCCTATGAGAAGTGTTATACCGATGTGACAGAGTTTGCCTTACCTGAAGGGAAGCTTTATCTATCGCCAGTTTTTGATGGCTATAACTG
TGAGATTATTGACTTTACCTTATCTCGCTCGCCTGACTTGAAACAGGTTCAAACGATGCTTGAGAAGGCTTTTCCAGCGGATTCATACAGTGGAACTATT
CTCCACAGCGACCAAGGGTGGCAGTACCAGCACCAGTCTTACCATCAGTTTTTGGAAACCAAAGGGATTCGTCCCTCTATGTCTCGAAAGGGAAATAGTC
CAGATAATGGGATGATGGAATCCTTCTTTGGCATTCTCAAATCGGAGATGTTCTATGGACTTGAGAAGTCTTACAAAACCCTTGATGAGCTTGAACAAGC
TATCACAGATTACATTTTTTACTACAACAACAAACGAATCAAAGCAAAACTAAAAGGACTTAGTCCTGTTCAATACAGAACCAAATCCTTGCAATAATTA
ATTGTCCAACTTCTGGGGGTCAGTACA
Recoding section
  • Recoding by frameshift
  • Frame -1
  • Type translational
  • Experimentally demonstrated No

Stimulators :

  • Shine-Dalgarno sequence :
  • Secondary structure :

Recoding motif :

Protein section
ORF number : 3

 

ORF 1
LengthBeginEndStrandFusion ORF
516 bp171 aa55570+No
ORF function : Transposase
Description : First part of the transposase

ORF sequence :

MKLSYEDKLEIYELKKSGVSWTNLSQTYKVTIANLTYMIKLMDRYGLEIVEKGKNRYYSLELKQEMIDKVLIHGCSQLSVSLDYTLPNRGMLPNWIAQYK
KNGYTILEKPRGRPAKMGRKPKKKLEEMTELERLQKELEYLRAENAVLKKLREYRLRDEAKLKEQQKSFKH

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
954 bp317 aa4441397+No
ORF function : Transposase
Description : Second part of the transposase

ORF sequence :

IGTSSERIRILKSGECCAKKAERIPLEGRSKAQRATEIIQALTNRFSLETLLEILDLPRSTYYYQVKRLAQGDKDIELKNLIREIYDEHKGNYGYRRIYL
ELRNRGFLINHKKVQRLMTVMGLAARIRRKRKYSSYKGEVGKKADNLIKRQFEGSKPYEKCYTDVTEFALPEGKLYLSPVFDGYNCEIIDFTLSRSPDLK
QVQTMLEKAFPADSYSGTILHSDQGWQYQHQSYHQFLETKGIRPSMSRKGNSPDNGMMESFFGILKSEMFYGLEKSYKTLDELEQAITDYIFYYNNKRIK
AKLKGLSPVQYRTKSLQ

 

Blast result :
ORF 3
LengthBeginEndStrandFusion ORF
1343 bp447 aa551397+Yes
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MKLSYEDKLEIYELKKSGVSWTNLSQTYKVTIANLTYMIKLMDRYGLEIVEKGKNRYYSLELKQEMIDKVLIHGCSQLSVSLDYTLPNRGMLPNWIAQYK
KNGYTILEKPRGRPAKMGRKPKKKLEEMTELERLQKELEYLRAENAVLKKAERIPLEGRSKAQRATEIIQALTNRFSLETLLEILDLPRSTYYYQVKRLA
QGDKDIELKNLIREIYDEHKGNYGYRRIYLELRNRGFLINHKKVQRLMTVMGLAARIRRKRKYSSYKGEVGKKADNLIKRQFEGSKPYEKCYTDVTEFAL
PEGKLYLSPVFDGYNCEIIDFTLSRSPDLKQVQTMLEKAFPADSYSGTILHSDQGWQYQHQSYHQFLETKGIRPSMSRKGNSPDNGMMESFFGILKSEMF
YGLEKSYKTLDELEQAITDYIFYYNNKRIKAKLKGLSPVQYRTKSLQ

 

Blast result :
Comments
ISStin7 is 92% aa similar to ISSeq3.
References
1] Alsheikh-Hussain A. (2017) Direct submission to ISfinder