ISStin8
- Family IS1182
- Group
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| CP017952 | ND | Streptococcus iniae | Streptococcus iniae 89353 Streptococcus iniae YSFST01-82 Streptococcus iniae ISNO Streptococcus iniae ISET0901 Streptococcus iniae SF1 Streptococcus iniae |
DNA section
IS Length : 1795 bp
Ends
IR Length : 24
IRL : GAGGCTGGGCAAAAAGTAGCTTCAGAATAGAAAAAACGAGCATTTCCGCA
IRR : GAGGCTGGGCAAAAAGTAGCTTCAAATAAAACCACGCAGAGATTTCAGTC
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| AATAGGCAAA | GTGAAGGAAA | 0 | |
| ATCATACAAA | TTTATAGTGC | 0 | |
| CATTTTAAAA | TTTTAGTGTA | 0 | |
| AAAACATTAT | AATCTTTAAA | 0 | |
| TATAAGAAGA | TTTTAAAGTA | 0 |
DNA sequence
GAGGCTGGGCAAAAAGTAGCTTCAGAATAGAAAAAACGAGCATTTCCGCAGTTGGAGATGCTCGTTTTCCTATGTCATGGTTTATAATTATAATAACGAC
AAAACAACTAGAAAGCCATGACTATGTATAAAGAGTATAACACAAATCAACTCAGTTTGGAATTAAATCTTGCCTACGATATTCCAATGAATCATGAGGT
TAGATTAATCAGTCTCTTTGTGGACAGTATTCCAAATCATATTTTGCTTGAAGAGACGTCTCACACTGGTCGTCCTGCCTTTCATCCTGCCATGCTTTTG
AAGATGACGCTCTTCGCCTATGCCCGCCAGGTCTTTTCTGGTCGCAAAATAGTTCAGATGAATGAAGAAGTCATTCCCATGAAATGGTTGAGCCAGGACA
CCTACGTTAGCTATAAAACCATCAATAATTTTCGTTCTAGTAAACATGCCAATAACCTAATTAAGACCGCCTTTATTTATTTCACCCTCCTCATGCGTGA
GAATGGCATGATTGAAGATGATGCTCTCTTTATCGATGGCACCAAGCTAGAAGCTGATGCTAATCTCTACTCTTTCACTTGGAAAAGAGCTGTTGATAAA
TACGAAGAGGCACTAAACGGAAAAATCTCAGAACTCTATGACCAGCTGGTTCAAGAGGGGGTAAACCTAGCTCTGTCTAAAGAAGAATGCGAAACCAGCG
AGGGGTTAGTGCGCCTTTTAGAAGACACTGAACAGGCCTTGGCGGAAGTTGAGAAATCAATTTCTGAGGAACCAAAAGTTATCAAGGGAGGTTCTGCCAA
TAAACAAAAGCGACGTCGTATTAAGAAACTGCGCAACCAGTTGAGAAAGGATTATCTCCCTCGTAAGCAACGCTATGAGGAGGCTAGAGAGATTCTAGAA
GAGCGTAACTCTTTCTCCAAAACGGACCACGATGCCACTTTTATGCGAATGAAAGAGGACCACATGATGAATGGCCAGCTCAAACCTGGCTATAATGTTC
AAGCTGCCACCAATGGTCAATATGTTCTTGCATATGATATATTTCCAAATCCAACGGACACCCGAACCCTGAAACCATTTCTCCAATCCATTCAAACTCT
GGACCTCTTCCAAAATATTGTCGCCGATGCCGGTTATGGCAGTGAGGAGAATTATAGTTTTATCATTGATGAGTTGGAGAAATCTCCTCTGATTCCCTAT
GGCATGTACCAGAAGGAATTGACTAAGAAATATAAAAACAGTCCTACTAATCCAAATAATTGGGACTACCTTGAAGAAACAGACCAATTCATTAAACCAG
ATGGTGTTGTTTATTCCTTTAAATATTACTCTCGTAGAAAAGATAAGTATGGCTTTGAACGGGATTTCAAGATTTATGAGGCAGAAAAAATACAAAGCAC
GGAAGAGTTGGAAAAACTTTCCAAAACAGAAAGTGGTCGCCAGAAACAGATCCATTACAATCCAACTTGGAATTACTTTAAAGAACTCATAAAGGAAGAA
TTACATAGCGAAGATGGCTCTCGAATCTATGCCAAACGGAAAATCGATGTCGAACCCGTTTTTGGCCGATTGAAGAGTGTTTTTGGCGTGCGTAGAGTCC
ATGTCAGAGGCAATCAAGCCGTCCAAACGGAGGTTGGATTCCTCTTCATGAGTATGAATCTCACGAAATTAGCTAAGAATCTCGACTCTAAAACTTCAAA
TACTCAAAAACCACACAGTGATTTTTTCATCTTGATTGTTTTCAAGACTGAAATCTCTGCGTGGTTTTATTTGAAGCTACTTTTTGCCCAGCCTC
AAAACAACTAGAAAGCCATGACTATGTATAAAGAGTATAACACAAATCAACTCAGTTTGGAATTAAATCTTGCCTACGATATTCCAATGAATCATGAGGT
TAGATTAATCAGTCTCTTTGTGGACAGTATTCCAAATCATATTTTGCTTGAAGAGACGTCTCACACTGGTCGTCCTGCCTTTCATCCTGCCATGCTTTTG
AAGATGACGCTCTTCGCCTATGCCCGCCAGGTCTTTTCTGGTCGCAAAATAGTTCAGATGAATGAAGAAGTCATTCCCATGAAATGGTTGAGCCAGGACA
CCTACGTTAGCTATAAAACCATCAATAATTTTCGTTCTAGTAAACATGCCAATAACCTAATTAAGACCGCCTTTATTTATTTCACCCTCCTCATGCGTGA
GAATGGCATGATTGAAGATGATGCTCTCTTTATCGATGGCACCAAGCTAGAAGCTGATGCTAATCTCTACTCTTTCACTTGGAAAAGAGCTGTTGATAAA
TACGAAGAGGCACTAAACGGAAAAATCTCAGAACTCTATGACCAGCTGGTTCAAGAGGGGGTAAACCTAGCTCTGTCTAAAGAAGAATGCGAAACCAGCG
AGGGGTTAGTGCGCCTTTTAGAAGACACTGAACAGGCCTTGGCGGAAGTTGAGAAATCAATTTCTGAGGAACCAAAAGTTATCAAGGGAGGTTCTGCCAA
TAAACAAAAGCGACGTCGTATTAAGAAACTGCGCAACCAGTTGAGAAAGGATTATCTCCCTCGTAAGCAACGCTATGAGGAGGCTAGAGAGATTCTAGAA
GAGCGTAACTCTTTCTCCAAAACGGACCACGATGCCACTTTTATGCGAATGAAAGAGGACCACATGATGAATGGCCAGCTCAAACCTGGCTATAATGTTC
AAGCTGCCACCAATGGTCAATATGTTCTTGCATATGATATATTTCCAAATCCAACGGACACCCGAACCCTGAAACCATTTCTCCAATCCATTCAAACTCT
GGACCTCTTCCAAAATATTGTCGCCGATGCCGGTTATGGCAGTGAGGAGAATTATAGTTTTATCATTGATGAGTTGGAGAAATCTCCTCTGATTCCCTAT
GGCATGTACCAGAAGGAATTGACTAAGAAATATAAAAACAGTCCTACTAATCCAAATAATTGGGACTACCTTGAAGAAACAGACCAATTCATTAAACCAG
ATGGTGTTGTTTATTCCTTTAAATATTACTCTCGTAGAAAAGATAAGTATGGCTTTGAACGGGATTTCAAGATTTATGAGGCAGAAAAAATACAAAGCAC
GGAAGAGTTGGAAAAACTTTCCAAAACAGAAAGTGGTCGCCAGAAACAGATCCATTACAATCCAACTTGGAATTACTTTAAAGAACTCATAAAGGAAGAA
TTACATAGCGAAGATGGCTCTCGAATCTATGCCAAACGGAAAATCGATGTCGAACCCGTTTTTGGCCGATTGAAGAGTGTTTTTGGCGTGCGTAGAGTCC
ATGTCAGAGGCAATCAAGCCGTCCAAACGGAGGTTGGATTCCTCTTCATGAGTATGAATCTCACGAAATTAGCTAAGAATCTCGACTCTAAAACTTCAAA
TACTCAAAAACCACACAGTGATTTTTTCATCTTGATTGTTTTCAAGACTGAAATCTCTGCGTGGTTTTATTTGAAGCTACTTTTTGCCCAGCCTC
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1671 bp | 557 aa | 124 | 1794 | + | No |
Chemistry : DDE
ORF sequence :
MYKEYNTNQLSLELNLAYDIPMNHEVRLISLFVDSIPNHILLEETSHTGRPAFHPAMLLKMTLFAYARQVFSGRKIVQMNEEVIPMKWLSQDTYVSYKTI
NNFRSSKHANNLIKTAFIYFTLLMRENGMIEDDALFIDGTKLEADANLYSFTWKRAVDKYEEALNGKISELYDQLVQEGVNLALSKEECETSEGLVRLLE
DTEQALAEVEKSISEEPKVIKGGSANKQKRRRIKKLRNQLRKDYLPRKQRYEEAREILEERNSFSKTDHDATFMRMKEDHMMNGQLKPGYNVQAATNGQY
VLAYDIFPNPTDTRTLKPFLQSIQTLDLFQNIVADAGYGSEENYSFIIDELEKSPLIPYGMYQKELTKKYKNSPTNPNNWDYLEETDQFIKPDGVVYSFK
YYSRRKDKYGFERDFKIYEAEKIQSTEELEKLSKTESGRQKQIHYNPTWNYFKELIKEELHSEDGSRIYAKRKIDVEPVFGRLKSVFGVRRVHVRGNQAV
QTEVGFLFMSMNLTKLAKNLDSKTSNTQKPHSDFFILIVFKTEISAWFYLKLLFAQP
NNFRSSKHANNLIKTAFIYFTLLMRENGMIEDDALFIDGTKLEADANLYSFTWKRAVDKYEEALNGKISELYDQLVQEGVNLALSKEECETSEGLVRLLE
DTEQALAEVEKSISEEPKVIKGGSANKQKRRRIKKLRNQLRKDYLPRKQRYEEAREILEERNSFSKTDHDATFMRMKEDHMMNGQLKPGYNVQAATNGQY
VLAYDIFPNPTDTRTLKPFLQSIQTLDLFQNIVADAGYGSEENYSFIIDELEKSPLIPYGMYQKELTKKYKNSPTNPNNWDYLEETDQFIKPDGVVYSFK
YYSRRKDKYGFERDFKIYEAEKIQSTEELEKLSKTESGRQKQIHYNPTWNYFKELIKEELHSEDGSRIYAKRKIDVEPVFGRLKSVFGVRRVHVRGNQAV
QTEVGFLFMSMNLTKLAKNLDSKTSNTQKPHSDFFILIVFKTEISAWFYLKLLFAQP
Blast result :
Comments
ISStin8 is 92% aa similar to ISSmi2.
There is no stop codon at the end of the element, the transposase use the first Stop codon after the end of the IRR.
The transposase in the database is cut at the end of the IS.
There is no stop codon at the end of the element, the transposase use the first Stop codon after the end of the IRR.
The transposase in the database is cut at the end of the IS.
References
1] Alsheikh-Hussain A. (2017) Direct submission to ISfinder