ISBm2
- Family IS3
- Group IS407
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| AF047478 | ND | Brucella melitensis | Brucella melitensis 16M |
DNA section
IS Length : 1274 bp
Ends
IR Length : 11/12
IRL : TGACCTTGCCCCGTTGAAATAATCCATTTTGAAGTAAGCTCTGGCCCATT
IRR : TGACGTTGCCCCCCAAGTTTTATCCAGTTTTGAGTTCGCGCCAGCGGTTT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|
DNA sequence
TGACCTTGCCCCGTTGAAATAATCCATTTTGAAGTAAGCTCTGGCCCATTGAGAGGACCAGAGAATGAAGTGCAACCGTTTTACAGACGGAACAGATCAT
CGGCATTCTGAAGGAGCACGAGGCGGGCACGCCTGTCGCGGAGCTTTGTCGCAAGCACGGCGTCAGCGATGCCAGCATCTATAAATGGAAAGCCAAGTTC
GGCGGCATGGACGTGTCCGAGGCCAAGCGGTTGAAGACGCTGGAAGACGAAAACACAAAGCTGAAACGGCTCCTGGCAGATGCGATGCTCGACAACGCCG
CTTTGAAAGACCTTTTGGGAAAGAAGTGGTGACACCCGCAGCAAGCCGGAAAGCTGTCGCTCATCTGATGGATCATCACCAGATGAGCGAACGGCGGACG
TGTAAAGCCATGGGCTTTTGCTGTATGGCGATCCGTTACGAAACCAGGCGCAGCGATGACCATGACCTTCGCGAGCGGATGAAGGCGCTAGCGCATGAAC
GTCGCTGCTTTGGATATAGACGTCTTCAAGTGCTGCTCAGGCGTGAGGGTCACCTTGTGAACCACAAGTGGCTCTTCCGGCTCTATCGGGAGGAGAAGTT
GACGGTACGCAAGCGTGGCGGGCGTAAGCGGGCGATAGGCACCCGAGCGCCGATGTTGATCCCGCCGGCTGCCAATGTTCGCTGGTTGCTGGACTTCGTG
TCAGATCAGCTCACCGATGGCCGCAGGTTCCGGGTGCTTGCGGTCGTCGATGATTGCACAAGAGAATGCCTGCCTCTTGTCGCCGATACGTCCCTTTCCG
GCTTGCGGGTTGCCCGTGAGTTGGACCGGATCATCGAAAGACGTGGCAAGCCGAAGATGATCGTCAGTGACAACGGCAGTGAGTTCACCAGTAACGCAAT
CCTGCATTGGGTGGATCGGACCAAAATGGAATGGCACTATATAGCGCCTGGCAAACCGATCCAGAACGCCTTCATCGAAAGCTTCAATGGACAGCTACGA
GACGAGCTCCTGAATGAAACGTTCTTCTCGTCACTAACCCATGCATGATCAGCGCTTTTAAACTGGCGCAGCGATTACAACGATCATCGACCACACTCTG
AACTCGGCTGGATGACGCCTGCCGAGTTCGCTCAGACAATCAACCCGCGACGTGATGCGGTGCTGCGCAGCCCGGACGACTCCGCACCGCAACCCGCCGC
TACCGCCCCGAATACAACAACCCAAAACCGCTGGCGCGAACTCAAAACTGGATAAAACTTGGGGGGCAACGTCA
CGGCATTCTGAAGGAGCACGAGGCGGGCACGCCTGTCGCGGAGCTTTGTCGCAAGCACGGCGTCAGCGATGCCAGCATCTATAAATGGAAAGCCAAGTTC
GGCGGCATGGACGTGTCCGAGGCCAAGCGGTTGAAGACGCTGGAAGACGAAAACACAAAGCTGAAACGGCTCCTGGCAGATGCGATGCTCGACAACGCCG
CTTTGAAAGACCTTTTGGGAAAGAAGTGGTGACACCCGCAGCAAGCCGGAAAGCTGTCGCTCATCTGATGGATCATCACCAGATGAGCGAACGGCGGACG
TGTAAAGCCATGGGCTTTTGCTGTATGGCGATCCGTTACGAAACCAGGCGCAGCGATGACCATGACCTTCGCGAGCGGATGAAGGCGCTAGCGCATGAAC
GTCGCTGCTTTGGATATAGACGTCTTCAAGTGCTGCTCAGGCGTGAGGGTCACCTTGTGAACCACAAGTGGCTCTTCCGGCTCTATCGGGAGGAGAAGTT
GACGGTACGCAAGCGTGGCGGGCGTAAGCGGGCGATAGGCACCCGAGCGCCGATGTTGATCCCGCCGGCTGCCAATGTTCGCTGGTTGCTGGACTTCGTG
TCAGATCAGCTCACCGATGGCCGCAGGTTCCGGGTGCTTGCGGTCGTCGATGATTGCACAAGAGAATGCCTGCCTCTTGTCGCCGATACGTCCCTTTCCG
GCTTGCGGGTTGCCCGTGAGTTGGACCGGATCATCGAAAGACGTGGCAAGCCGAAGATGATCGTCAGTGACAACGGCAGTGAGTTCACCAGTAACGCAAT
CCTGCATTGGGTGGATCGGACCAAAATGGAATGGCACTATATAGCGCCTGGCAAACCGATCCAGAACGCCTTCATCGAAAGCTTCAATGGACAGCTACGA
GACGAGCTCCTGAATGAAACGTTCTTCTCGTCACTAACCCATGCATGATCAGCGCTTTTAAACTGGCGCAGCGATTACAACGATCATCGACCACACTCTG
AACTCGGCTGGATGACGCCTGCCGAGTTCGCTCAGACAATCAACCCGCGACGTGATGCGGTGCTGCGCAGCCCGGACGACTCCGCACCGCAACCCGCCGC
TACCGCCCCGAATACAACAACCCAAAACCGCTGGCGCGAACTCAAAACTGGATAAAACTTGGGGGGCAACGTCA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 225 bp | 74 aa | 108 | 332 | + | No |
Description : First part of the transposase
ORF sequence :
MKEHEAGTPVAELCRKHGVSDASIYKWKAKFGGMDVSEAKRLKTLEDENTKLKRLLADAMLDNAALKDLLGKKW
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 864 bp | 286 aa | 185 | 1048 | + | No |
Description : Second part of the transposase
ORF sequence :
MESQVRRHGRVRGQAVEDARRKHKAETAPGRCDARQRRFERPFGKEVVTPAASRKAVAHLMDHHQMSERRTCKAMGFCCMAIRYETRRSDDHDLRERMKA
LAHERRCFGYRRLQVLLRREGHLVNHKWLFRLYREEKLTVRKRGGRKRAIGTRAPMLIPPAANVRWLLDFVSDQLTDGRRFRVLAVVDDCTRECLPLVAD
TSLSGLRVARELDRIIERRGKPKMIVSDNGSEFTSNAILHWVDRTKMEWHYIAPGKPIQNAFIESFNGQLRDELLNETFFSSLTHA
LAHERRCFGYRRLQVLLRREGHLVNHKWLFRLYREEKLTVRKRGGRKRAIGTRAPMLIPPAANVRWLLDFVSDQLTDGRRFRVLAVVDDCTRECLPLVAD
TSLSGLRVARELDRIIERRGKPKMIVSDNGSEFTSNAILHWVDRTKMEWHYIAPGKPIQNAFIESFNGQLRDELLNETFFSSLTHA
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 941 bp | 313 aa | 108 | 1048 | + | Yes |
Chemistry : DDE
ORF sequence :
MKEHEAGTPVAELCRKHGVSDASIYKWKAKFGGMDVSEAKRLKTLEDENTKLKRLLADAMLDNAALKDLLGKEVVTPAASRKAVAHLMDHHQMSERRTCK
AMGFCCMAIRYETRRSDDHDLRERMKALAHERRCFGYRRLQVLLRREGHLVNHKWLFRLYREEKLTVRKRGGRKRAIGTRAPMLIPPAANVRWLLDFVSD
QLTDGRRFRVLAVVDDCTRECLPLVADTSLSGLRVARELDRIIERRGKPKMIVSDNGSEFTSNAILHWVDRTKMEWHYIAPGKPIQNAFIESFNGQLRDE
LLNETFFSSLTHA
AMGFCCMAIRYETRRSDDHDLRERMKALAHERRCFGYRRLQVLLRREGHLVNHKWLFRLYREEKLTVRKRGGRKRAIGTRAPMLIPPAANVRWLLDFVSD
QLTDGRRFRVLAVVDDCTRECLPLVADTSLSGLRVARELDRIIERRGKPKMIVSDNGSEFTSNAILHWVDRTKMEWHYIAPGKPIQNAFIESFNGQLRDE
LLNETFFSSLTHA
Blast result :
Comments
The ORF1 of ISBm2 is 87% aa similar to ISR1, 80% to IS3 (ORFA) and 83% to IS407 (ORFA), and the ORF2 of this IS is 60% similar to ISRm6, 62% to IS1389 (ORFB), 57% to IS1222 (ORFB).
References
1] Godfroid,F., Taminiau,B., Danese,I., Denoel,P., Tibor,A.,Weynants,V., Cloeckaert,A., Godfroid,J. and Letesson,J.-J.(1998) Infect. Immun. 66 (11) (1998) In press
2] Godfroid,F. and Letesson,J.-J. (1998) Direct Submission
2] Godfroid,F. and Letesson,J.-J. (1998) Direct Submission