ISStin6
- Family IS3
- Group IS150
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| CP017952 | ND | Streptococcus iniae | Streptococcus iniae 89353 Streptococcus iniae YSFST01-82 Streptococcus iniae ISNO Streptococcus iniae ISET0901 Streptococcus iniae SF1 Streptococcus iniae |
DNA section
IS Length : 1219 bp
Ends
IR Length : 29/41
IRL : TGAATCGGTTACACTAAACTAGACAGAATTTATAAAGTGTTCTACACTAA
IRR : TGAAAAGGCAACAGTTTTCTGTACAAGATTTATAAAGTGTTTTTATTAGA
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| CCAGAATTCA | AAT | CATGAAGCAA | 3 |
| CTTTCCTGTT | ATC | CTAACGGATA | 3 |
DNA sequence
TGAATCGGTTACACTAAACTAGACAGAATTTATAAAGTGTTCTACACTAAAGAAAATAGGAGAATATGATGTCTAGAAAAATACGTCGCCACTTCACTGA
TGACTTTAAGCAACAAATCGTTGACCTTCACCATGCAGGGATGAAACGAAGTGAGCTTATCAAAGAATATGAGTTAACCCCATCAACCTTCGATAAGTGG
GTTCGTCAGGCAAAAACAACTGGGTCATTTAAAACGATTGATAATCTGACAGATGAACAGCGTGAACTGATTGAACTCCGAAAACGCAATAAAGAACTCG
AAATGCAGCTAGATATCCTAAAGCAAGCGGCAGTGATTATGGCACGAAAAGAGAAGTAATCACTGCTAATAAGGATAAATATAGCATTTCAGCTATGTGT
CGTTGGTTGAACATTCCTCGTTCTAGCTATTACTACAAGACTGTTAAACCAGTCTCTGAAGCGGAACTTGAAGAAAATATCAAAGCTATTTTTCTCGAAA
GTAAGGCCAGATACGGTGCTAGGAAAATCAAGAAATGTTTGGAAAATGAAGGTATCCAGCGGTCTCGTCGTCGGATTCGTCGCATCATGCACAGACTCAA
CTTAGTATCTGTTTATCAGAAAGCAGTCTTCAAGCCACATTCAAAAGGTAAGAATGAGGTAGCTATTCCTAATCACTTAGCCAGACAATTTCATCAAGAA
AAGCCTCTAAAAGCTTTAGTGACAGACTTAACCTACGTTCGTGTCGGTAATGGCTGGGCTTATGTTTGTTTCATTATGGACCTCTACAACCGTGAAATCA
TTGGCTTATCGGTTGGTTGGCACAAGACAGCAGAGCTGGTGAAACAAGCGATTCAGAGTATTCCTTACGCTCTGACCAAGGTCAAGATATTCCATTCGGA
CAGAGGAAAAGAGTTCGACAATGCCTTGATAGATGATGTGCTTACAGCCTTTGATATCAAACGCTCACTTAGTCAAGCAGGTTGTCCTTACGACAATGCC
GTAGCTGAGAGCACCTATCGCGCTTTCAAAATAGAATTTGTCCACCAGGAAACTTTCCAGACGCTAGAAGAATTGGCCCTTAAAACCAAGGACTATGTCC
ACTGGTGGAATCACCACCGCATTCATGGTAGTCTAAACTATCAGACGCCCATGACCAAAAGATTAACGGTCTAATAAAAACACTTTATAAATCTTGTACA
GAAAACTGTTGCCTTTTCA
TGACTTTAAGCAACAAATCGTTGACCTTCACCATGCAGGGATGAAACGAAGTGAGCTTATCAAAGAATATGAGTTAACCCCATCAACCTTCGATAAGTGG
GTTCGTCAGGCAAAAACAACTGGGTCATTTAAAACGATTGATAATCTGACAGATGAACAGCGTGAACTGATTGAACTCCGAAAACGCAATAAAGAACTCG
AAATGCAGCTAGATATCCTAAAGCAAGCGGCAGTGATTATGGCACGAAAAGAGAAGTAATCACTGCTAATAAGGATAAATATAGCATTTCAGCTATGTGT
CGTTGGTTGAACATTCCTCGTTCTAGCTATTACTACAAGACTGTTAAACCAGTCTCTGAAGCGGAACTTGAAGAAAATATCAAAGCTATTTTTCTCGAAA
GTAAGGCCAGATACGGTGCTAGGAAAATCAAGAAATGTTTGGAAAATGAAGGTATCCAGCGGTCTCGTCGTCGGATTCGTCGCATCATGCACAGACTCAA
CTTAGTATCTGTTTATCAGAAAGCAGTCTTCAAGCCACATTCAAAAGGTAAGAATGAGGTAGCTATTCCTAATCACTTAGCCAGACAATTTCATCAAGAA
AAGCCTCTAAAAGCTTTAGTGACAGACTTAACCTACGTTCGTGTCGGTAATGGCTGGGCTTATGTTTGTTTCATTATGGACCTCTACAACCGTGAAATCA
TTGGCTTATCGGTTGGTTGGCACAAGACAGCAGAGCTGGTGAAACAAGCGATTCAGAGTATTCCTTACGCTCTGACCAAGGTCAAGATATTCCATTCGGA
CAGAGGAAAAGAGTTCGACAATGCCTTGATAGATGATGTGCTTACAGCCTTTGATATCAAACGCTCACTTAGTCAAGCAGGTTGTCCTTACGACAATGCC
GTAGCTGAGAGCACCTATCGCGCTTTCAAAATAGAATTTGTCCACCAGGAAACTTTCCAGACGCTAGAAGAATTGGCCCTTAAAACCAAGGACTATGTCC
ACTGGTGGAATCACCACCGCATTCATGGTAGTCTAAACTATCAGACGCCCATGACCAAAAGATTAACGGTCTAATAAAAACACTTTATAAATCTTGTACA
GAAAACTGTTGCCTTTTCA
Recoding section
- Recoding by frameshift
- Frame -1
- Type translational
- Experimentally demonstrated No
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 291 bp | 96 aa | 69 | 359 | + | No |
Description : First part of the transposase
ORF sequence :
MSRKIRRHFTDDFKQQIVDLHHAGMKRSELIKEYELTPSTFDKWVRQAKTTGSFKTIDNLTDEQRELIELRKRNKELEMQLDILKQAAVIMARKEK
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 882 bp | 293 aa | 293 | 1174 | + | No |
Description : Second part of the transposase
ORF sequence :
RTRNAARYPKASGSDYGTKREVITANKDKYSISAMCRWLNIPRSSYYYKTVKPVSEAELEENIKAIFLESKARYGARKIKKCLENEGIQRSRRRIRRIMH
RLNLVSVYQKAVFKPHSKGKNEVAIPNHLARQFHQEKPLKALVTDLTYVRVGNGWAYVCFIMDLYNREIIGLSVGWHKTAELVKQAIQSIPYALTKVKIF
HSDRGKEFDNALIDDVLTAFDIKRSLSQAGCPYDNAVAESTYRAFKIEFVHQETFQTLEELALKTKDYVHWWNHHRIHGSLNYQTPMTKRLTV
RLNLVSVYQKAVFKPHSKGKNEVAIPNHLARQFHQEKPLKALVTDLTYVRVGNGWAYVCFIMDLYNREIIGLSVGWHKTAELVKQAIQSIPYALTKVKIF
HSDRGKEFDNALIDDVLTAFDIKRSLSQAGCPYDNAVAESTYRAFKIEFVHQETFQTLEELALKTKDYVHWWNHHRIHGSLNYQTPMTKRLTV
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1106 bp | 368 aa | 69 | 1174 | + | Yes |
Chemistry : DDE
ORF sequence :
MSRKIRRHFTDDFKQQIVDLHHAGMKRSELIKEYELTPSTFDKWVRQAKTTGSFKTIDNLTDEQRELIELRKRNKELEMQLDILKQAAVIMARKREVITA
NKDKYSISAMCRWLNIPRSSYYYKTVKPVSEAELEENIKAIFLESKARYGARKIKKCLENEGIQRSRRRIRRIMHRLNLVSVYQKAVFKPHSKGKNEVAI
PNHLARQFHQEKPLKALVTDLTYVRVGNGWAYVCFIMDLYNREIIGLSVGWHKTAELVKQAIQSIPYALTKVKIFHSDRGKEFDNALIDDVLTAFDIKRS
LSQAGCPYDNAVAESTYRAFKIEFVHQETFQTLEELALKTKDYVHWWNHHRIHGSLNYQTPMTKRLTV
NKDKYSISAMCRWLNIPRSSYYYKTVKPVSEAELEENIKAIFLESKARYGARKIKKCLENEGIQRSRRRIRRIMHRLNLVSVYQKAVFKPHSKGKNEVAI
PNHLARQFHQEKPLKALVTDLTYVRVGNGWAYVCFIMDLYNREIIGLSVGWHKTAELVKQAIQSIPYALTKVKIFHSDRGKEFDNALIDDVLTAFDIKRS
LSQAGCPYDNAVAESTYRAFKIEFVHQETFQTLEELALKTKDYVHWWNHHRIHGSLNYQTPMTKRLTV
Blast result :
Comments
ISStin6 is 94% aa similar to ISSdy1.
References
1] Alsheikh-Hussain A. (2017) Direct submission to ISfinder