ISStin5
- Family IS3
- Group IS150
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| CP017952 | ND | Streptococcus iniae | Streptococcus iniae SF1 Streptococcus iniae Streptococcus iniae strain 89353 Streptococcus iniae strain YSFST01-82 |
DNA section
IS Length : 1248 bp
Ends
IR Length : 30/38
IRL : GGCACTGCCCCCATCAAATGAGACAGAAGAAAAACACTCCTGAAGAAATC
IRR : GGCGCTGCACCCCAATAGTGGGACAGAAGAAAAACACTTTCACGCGATCA
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| AATAGAGAGA | ATC | ACCTCTCTTT | 3 |
| AAACAGCTAC | GAT | TGCGTGGCTG | 3 |
DNA sequence
GGCACTGCCCCCATCAAATGAGACAGAAGAAAAACACTCCTGAAGAAATCTAGTAAACTAGAAACAGGAGTGTTTTATTATGGTCAAAAAAGCTTATTCA
TTAGAAACGAAGTTATCTTGCATCGAGATGAAGAATGTAGGCAAGTCAAACAAGGTCATTATGGAGACCTTAGGTATCAAAAATGATAGTCAAATCTATA
CTTGGATGAAATGGTATGAAAAGGATGAATTGCACCGTTTCCACCAACCTGTAGGTAAACAATATACTTACGGACAAGGTATGGAACAACTCTCTGAAAT
AGAACAGTTACGACTTCAGGTGGATCTCTTAAAAAAGTATCGAAGCTTGATAAGAAAATCGACAAAGTGACCCTTATCAAGCTTGTGGAAGACTATAAGA
ATATTTACCCCGTTTCCGTTATTCTAAATTGTTTTGGTGTCAGACGGTCTACCTTTTACCGTTGGAAAAAGAAAAGGGAGACGCCACAGAAAAGAGATGT
CATAGTTGAAACCATTGAGCTACTCTGCATGGAGAATCACTTTATTTATGGTTATCGTACCATTACACGATTGCTCAAGAAGACCTATGGATTGACTATT
AATGCTAAGAAGGTCTATCGTATCATGAAAACTAATGGCTGGCTTTGTCGTACACGTCCAAAGAAATCTCCCAATCTGGGCAAACCCTATTATGTGACGG
GAAATAAATTAAATCGGAATTTCCAAGCCGATAAGCCATTGGAAAAGCTTGTAACGGATATTACCTACCTATATTTCGGCAACTGTAAATTGTATCTCTC
TTCAATTATGGACCTCTATAACCGAGAGATTATAGCTTATACTATCTCAGATTGTCAGGACACAGATTTTGTACTAGACACCCTTAATCAACTGAAATTA
CCCAATGGAGCACTCTTACACAGCGATCAGGGCTCAGTCTACACTTCTAAGGCTTACTATCAGGCTTGCACGGAAAAAGGCATCACCCGCTCTATGTCCC
GTAAGGGAACACCAGCAGATAATGCCTGTATTGAATGTTTTCACTCCGTCCTAAAGACTGAAACCTTTTATCTCCATGATAGGAGAAAATACAACAAGGA
TAGTATAACAAATATTGTTAAAAATTACATTATATTTTATAATGAAACTAGAATTCAACAGAGATTAAACGACCAGCCTCCTGTACAGTACAGGAAACTG
ATCGCGTGAAAGTGTTTTTCTTCTGTCCCACTATTGGGGTGCAGCGCC
TTAGAAACGAAGTTATCTTGCATCGAGATGAAGAATGTAGGCAAGTCAAACAAGGTCATTATGGAGACCTTAGGTATCAAAAATGATAGTCAAATCTATA
CTTGGATGAAATGGTATGAAAAGGATGAATTGCACCGTTTCCACCAACCTGTAGGTAAACAATATACTTACGGACAAGGTATGGAACAACTCTCTGAAAT
AGAACAGTTACGACTTCAGGTGGATCTCTTAAAAAAGTATCGAAGCTTGATAAGAAAATCGACAAAGTGACCCTTATCAAGCTTGTGGAAGACTATAAGA
ATATTTACCCCGTTTCCGTTATTCTAAATTGTTTTGGTGTCAGACGGTCTACCTTTTACCGTTGGAAAAAGAAAAGGGAGACGCCACAGAAAAGAGATGT
CATAGTTGAAACCATTGAGCTACTCTGCATGGAGAATCACTTTATTTATGGTTATCGTACCATTACACGATTGCTCAAGAAGACCTATGGATTGACTATT
AATGCTAAGAAGGTCTATCGTATCATGAAAACTAATGGCTGGCTTTGTCGTACACGTCCAAAGAAATCTCCCAATCTGGGCAAACCCTATTATGTGACGG
GAAATAAATTAAATCGGAATTTCCAAGCCGATAAGCCATTGGAAAAGCTTGTAACGGATATTACCTACCTATATTTCGGCAACTGTAAATTGTATCTCTC
TTCAATTATGGACCTCTATAACCGAGAGATTATAGCTTATACTATCTCAGATTGTCAGGACACAGATTTTGTACTAGACACCCTTAATCAACTGAAATTA
CCCAATGGAGCACTCTTACACAGCGATCAGGGCTCAGTCTACACTTCTAAGGCTTACTATCAGGCTTGCACGGAAAAAGGCATCACCCGCTCTATGTCCC
GTAAGGGAACACCAGCAGATAATGCCTGTATTGAATGTTTTCACTCCGTCCTAAAGACTGAAACCTTTTATCTCCATGATAGGAGAAAATACAACAAGGA
TAGTATAACAAATATTGTTAAAAATTACATTATATTTTATAATGAAACTAGAATTCAACAGAGATTAAACGACCAGCCTCCTGTACAGTACAGGAAACTG
ATCGCGTGAAAGTGTTTTTCTTCTGTCCCACTATTGGGGTGCAGCGCC
Recoding section
- Recoding by frameshift
- Frame -1
- Type transcriptional
- Experimentally demonstrated No
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 291 bp | 96 aa | 80 | 370 | + | No |
Description : First part of the transposase
ORF sequence :
MVKKAYSLETKLSCIEMKNVGKSNKVIMETLGIKNDSQIYTWMKWYEKDELHRFHQPVGKQYTYGQGMEQLSEIEQLRLQVDLLKKYRSLIRKSTK
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 912 bp | 0 aa | 298 | 1209 | + | No |
Description : Second part of the transposase
ORF sequence :
NRTVTTSGGSLKKVSKLDKKIDKVTLIKLVEDYKNIYPVSVILNCFGVRRSTFYRWKKKRETPQKRDVIVETIELLCMENHFIYGYRTITRLLKKTYGLT
INAKKVYRIMKTNGWLCRTRPKKSPNLGKPYYVTGNKLNRNFQADKPLEKLVTDITYLYFGNCKLYLSSIMDLYNREIIAYTISDCQDTDFVLDTLNQLK
LPNGALLHSDQGSVYTSKAYYQACTEKGITRSMSRKGTPADNACIECFHSVLKTETFYLHDRRKYNKDSITNIVKNYIIFYNETRIQQRLNDQPPVQYRK
LIA
INAKKVYRIMKTNGWLCRTRPKKSPNLGKPYYVTGNKLNRNFQADKPLEKLVTDITYLYFGNCKLYLSSIMDLYNREIIAYTISDCQDTDFVLDTLNQLK
LPNGALLHSDQGSVYTSKAYYQACTEKGITRSMSRKGTPADNACIECFHSVLKTETFYLHDRRKYNKDSITNIVKNYIIFYNETRIQQRLNDQPPVQYRK
LIA
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1130 bp | 376 aa | 80 | 1209 | + | Yes |
Chemistry : DDE
ORF sequence :
MVKKAYSLETKLSCIEMKNVGKSNKVIMETLGIKNDSQIYTWMKWYEKDELHRFHQPVGKQYTYGQGMEQLSEIEQLRLQVDLLKKVSKLDKKIDKVTLI
KLVEDYKNIYPVSVILNCFGVRRSTFYRWKKKRETPQKRDVIVETIELLCMENHFIYGYRTITRLLKKTYGLTINAKKVYRIMKTNGWLCRTRPKKSPNL
GKPYYVTGNKLNRNFQADKPLEKLVTDITYLYFGNCKLYLSSIMDLYNREIIAYTISDCQDTDFVLDTLNQLKLPNGALLHSDQGSVYTSKAYYQACTEK
GITRSMSRKGTPADNACIECFHSVLKTETFYLHDRRKYNKDSITNIVKNYIIFYNETRIQQRLNDQPPVQYRKLIA
KLVEDYKNIYPVSVILNCFGVRRSTFYRWKKKRETPQKRDVIVETIELLCMENHFIYGYRTITRLLKKTYGLTINAKKVYRIMKTNGWLCRTRPKKSPNL
GKPYYVTGNKLNRNFQADKPLEKLVTDITYLYFGNCKLYLSSIMDLYNREIIAYTISDCQDTDFVLDTLNQLKLPNGALLHSDQGSVYTSKAYYQACTEK
GITRSMSRKGTPADNACIECFHSVLKTETFYLHDRRKYNKDSITNIVKNYIIFYNETRIQQRLNDQPPVQYRKLIA
Blast result :
Comments
ISStin5 is 66% aa similar to ISLmo1.
References
1] Alsheikh-Hussain A. (2017) Direct submission to ISfinder