ISfinder Mirror Site

ISTesp4

Family IS1595
Group ISSod11

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
LMIW01000008	ND	Terrabacter sp.	Terrabacter sp. Root85

DNA section

IS Length : 1005 bp

Ends

IR Length : 23/24

IRL : TCTGCATATCTGCTTGGGTGAAGTAGGATCTGTGGATGACCGCGAATCCG
IRR : TCTGCATATCTGCTTAGGTGAAGTCCGGTATGCCGGTTTCTGGCTTGAGC

Insertion site

Left flank	Direct repeat	Right flank	DR Length
GTAGAATCGA	ACACGTGT	TCGAAGGTGT	8

DNA sequence

TCTGCATATCTGCTTGGGTGAAGTAGGATCTGTGGATGACCGCGAATCCGGTTCGGGAAGTGGACTACCCGTCCACCTACCAGCAGCTGCTGGAGTGGTT
CCCGGACGACCAGTCCTGCCTGGACTACCTGGCCGGACTGCGCTGGCCCGACGGATTCGTGTGCCCGAAGTGCGAAGCCACTGAGTTCTGGCGCACCGGT
GCCGGGCTGTGGATGTGCCGGGCCTGCGGGCGGCGCACGTCGGTCACCGCGGGAACGATCTTCGACCGGACGCGGACGCCGTTGTCGACCTGGTTCGCGG
CGATCTGGTTCCTGACCTCGCAGAAGAACGGGATGTCCGCACTCGGCCTGCAACGGCTCCTCGGGTTCGGCTCCTACGAGACGGCGTGGGCCTGGATGCA
CAAGCTGCGCCGGGCGATGGTCCGCCCGGAGCGGGACAAGCTGTCCGGGATCGTGGAGCTCGACGAGACGATGATCGGCGGCGTCACCCGCGGCCGGACG
GGCGGCTACGGCGACAAGGTCGCGGTCATGATCGCCATAGAACACAACGAAACCCGCCGCCTGGGCCGAGTCCGGCTCGGCGTCGCCACCCAGCCCGGAA
CCACCGAGCTGGTCGACTTCGCCACCCGGGTCATCGAGCCCGGATCAACGATCCGCACCGACGGCGCCCGCACCCTGCGCCGGCTCGCCGACCTCGGCTA
CACCCACGAGTTCACCCTCGGCTACTCCGCGCCCGACAAGGCCAGCGTGCTGCCCGGAGTGCACCTGGTGGCCTCACTGCTCAAGCGCTGGCTGATCGGC
ACCCTGCACTTCCGCGTCGAGGAGGCACACCTGCCCTACTACCTCGACGAGTACACCTTCCGGTTCAACCGCCGCAACGCGAAGTCACGAGGACTGCTCT
TCTATCGGCTGCTCGAGCAAGCCGTCGACACCGACCCGCACCCGCTGAAGGGCCTGCTCAAGCCAGAAACCGGCATACCGGACTTCACCTAAGCAGATAT
GCAGA

Protein section

ORF number : 1

ORF 1

Length		Begin	End	Strand	Fusion ORF
957 bp	318 aa	36	992	+	No

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MTANPVREVDYPSTYQQLLEWFPDDQSCLDYLAGLRWPDGFVCPKCEATEFWRTGAGLWMCRACGRRTSVTAGTIFDRTRTPLSTWFAAIWFLTSQKNGM
SALGLQRLLGFGSYETAWAWMHKLRRAMVRPERDKLSGIVELDETMIGGVTRGRTGGYGDKVAVMIAIEHNETRRLGRVRLGVATQPGTTELVDFATRVI
EPGSTIRTDGARTLRRLADLGYTHEFTLGYSAPDKASVLPGVHLVASLLKRWLIGTLHFRVEEAHLPYYLDEYTFRFNRRNAKSRGLLFYRLLEQAVDTD
PHPLKGLLKPETGIPDFT

Blast result :

Comments

ISTesp4 is 77% aa similar to ISSat1.

References

1] ISfinder annotation (2017)
2] Bai,Y., Muller,D.B., Srinivas,G., Garrido-Oter,R., Potthoff,E., Rott,M., Dombrowski,N., Munch,P.C., Spaepen,S., Remus-Emsermann,M., Huttel,B., McHardy,A.C., Vorholt,J.A. and Schulze-Lefert,P. (2015) Nature 528 (7582), 364-369.