ISfinder Mirror Site

ISPa72

Family IS1595
Group IS1595

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
MPBP01000001	ND	Pseudomonas aeruginosa	Pseudomonas aeruginosa AR_0103

DNA section

IS Length : 1048 bp

Ends

IR Length : 21/23

IRL : CCTGATTAGAAACCTGCCTCAGCGAGGCTCAACCCATGAGCTTGTGACCC
IRR : CCTGATTAGAAACGTGTCTCAGCATGCGACCTGACCGCCTTTTCCTGGAT

Insertion site

Left flank	Direct repeat	Right flank	DR Length
AGGCTGGCTT	TTTCTTGT	TATCGCAATA	8

DNA sequence

CCTGATTAGAAACCTGCCTCAGCGAGGCTCAACCCATGAGCTTGTGACCCACTAATCTGGAGTCCTCTTACAGTGGGGTGAGCATCATGGCCTTCAACCA
GATCCAGTTCCAGCACGGCATGTCGATTCCGGAATTCCTTCGTAGATTCGGCACTGAGGCACAGTGCGCCGAAGCGATCAAGGCCGCGCGCTGGCCTGAT
GGATTTCGCTGTCCGCGCTGCGCCAGCAGTGATCACTATGTGGTCGGCCACGGCGCCCGCAAGCTCTTCCAGTGCAATGGCTGTCGGCATCAGACCTCCC
TGACCGCAGGCAGTCTCATGGCGCACACCAAACTGCCCCTGACGACCTGGTTCTTGGCCATCTACCTCATCAGTCAGGCTAAGACAGGGCTGTCGGCCCT
GGCGCTCAAGCGTCAGCTTGGTGTGAGTTACCCCACGGCCTGGCTGTTGCATCAGAAGATCAACCGGGCCATGGCCGCGCAAGACGCCACCCACCAACTC
AGCGGCACGGTGCAGCTTGATGACGCTTACCTCGGAGGCGAGCGTACAGGCGGCAAAGTCGGTCGCGGCTCCGAGAACAAGGTTCCCTTTGTCGCGGCGG
TCTCGGTCAATGATCACGGGCATCCGATTTTCATCAAGCTCAATGTGGTCAGAGGGTTCACATCCGAAGCGATCAGCAAGTGGGCCAAGTCCAATCTGGC
ACCGGGCACCAGCGTGCACAGCGATGGTTTGGCCTGCTTCAGTGCGGTGGCCGATGCCGGTTGCCTTCACCTTCCGATGGTGGTCGGCGGCCTCAAGCCG
CGCGACCTGCCCAGCTTCAAGTGGGTCAACACCGTGCTTGGCAATCTCAAGACCACGTTGGCCGGAGCATTTCATGCCCTGAACTACCGCAAGTACGCCG
AGCACTACCTCGCAGGCTTCGCCTACAGGTTCAACCGCCGCTTCGATTTGCGGGGCCTGGTCGCAAGCCTCATCGTCGATGTCGTGAGAGCAAAGCCCAT
CCAGGAAAAGGCGGTCAGGTCGCATGCTGAGACACGTTTCTAATCAGG

Protein section

ORF number : 1

ORF 1

Length		Begin	End	Strand	Fusion ORF
957 bp	318 aa	87	1043	+	No

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MAFNQIQFQHGMSIPEFLRRFGTEAQCAEAIKAARWPDGFRCPRCASSDHYVVGHGARKLFQCNGCRHQTSLTAGSLMAHTKLPLTTWFLAIYLISQAKT
GLSALALKRQLGVSYPTAWLLHQKINRAMAAQDATHQLSGTVQLDDAYLGGERTGGKVGRGSENKVPFVAAVSVNDHGHPIFIKLNVVRGFTSEAISKWA
KSNLAPGTSVHSDGLACFSAVADAGCLHLPMVVGGLKPRDLPSFKWVNTVLGNLKTTLAGAFHALNYRKYAEHYLAGFAYRFNRRFDLRGLVASLIVDVV
RAKPIQEKAVRSHAETRF

Blast result :

Comments

ISPa72 is 80% aa similar to ISCap3.

References

1] ISfinder annotation (2017)
2] Earl,A., Limbago,B., Yoo,B., Ma,P., Battacharyya,R., Hung,D., Mccowan,C., Young,S., Abouelleil,A., Bochicchio,J., Cao,P., Chapman,S., Cusick,C., Shea,T., Neafsey,D., Nusbaum,C. and Birren,B. (2016) Direct GenBank submission.