ISBf2

  • Family IS21
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
AP006841 ND Bacteroides fragilis
Bacteroides fragilis YCH46
Bacteroides fragilis RBF49
DNA section
IS Length : 2544 bp

Ends


IR Length : 11/17

IRL : TGTAGGCTGTAACTTTATCTTTCAAAAAGTGGAAAAAGCAAAGGTACCAC
IRR : TGTACCGAGCAAATTTACAGGTCCCTTTTTTGTGCAATTTAACAGTATCC

Insertion site


Left flankDirect repeatRight flankDR Length
TCATGTATAATCCAATAATTATTTTTTT8

DNA sequence

TGTAGGCTGTAACTTTATCTTTCAAAAAGTGGAAAAAGCAAAGGTACCACTTTTATAGAGACGAATACAGTGTTTTTTGCATGTCGGGGAATGTCTATCT
TTGCCGCTCAAACAAAGCATAGACAGATACTGCAATGGAAATAAGGAAACAATATTTTAAGGAGAAACAATATATGTGGCATAAAGTAAGGGAGCTTCAG
TTGAAAGGACTGAACAAAACACAAATTGGAATATATCTGGGTGTGAACCGTAAGACTGTACGACGGTATCTGAATATGACTATGGAGGAGTTTGTTAAAA
AACAAAGTTCTCACCGCAAGTACAGGCTGAAACTGGAAAACTACGAGCAATATGTACGTGCAAACCTGGAAGAATACCCGTATATATCGGCCGCCAGGAT
ACATGACTGGCTGAAGGAATGCTATCCGGACTTCCCCCGTGTATGTAACAGAACCATATCCGGTTTCGTGGAAAGGGTACGCAAAAAATACGGCATCGGG
AAAAAGGTTGAAACGCATAAGCGAAACTACGAGAAGCAGCCTGATACTCCCTACGGGGAATACGCACAGGCGGATTTTGGGGAGAAATGTATGCGCACTG
AAAACGGGAAGTCCATCAAAGTGTACTTCTTTGCTATTGTCCTGTCCCGTTCACGATATAAATTTATCTATTTTAGCCGGAGGCCCTTTGACACCGGGCT
TGCGGTTTATGCCCATGAACTTGCCTTCGAATACTTCGGAGGCAGGCCGCAAAAGATCATTTACGACCAGGATAAAGTACTTATAGCACGGGCGAACATG
GGGGATTTGATACTGACCGGCAAATTTCAGGCATTTGTAAAAGAGCAGCATTTCCATCCCGTGTTCTGTCACAAGGCCGATCCGGAGTCAAAGGGGAGGG
TAGAGAATGTTGTGAAATATGTGAAGACGAATTTCCTCACGGCACGTATTTTTCAGAATGTAGACAGACTTAATGAAGAAGCACGTCTCTGGCTTGAAAG
AACGGGAAACGGGAAGGAATATGGTACCACACACCGGATTCCCCTTGAGGAATTTGCACAGGAAAGAGAATACCTTGTACCCTATCACGGTACTCCGCAC
TCACCCGGTGGAGAAATGAAGGAATATCATGTACGTAAAGACAATACCGTACAGTACAGGGGAAACTACTATAGCCTGCCATGCGGAACCTATCGGGGAG
GAGAAACGACAGTATGGCTCCATGAAACAGACGGATGCCTGGAACTTTATAATAAGGAGACGGGAAAGCTTGTCTGCCGGCATGATCTGTGCGAACTCAA
GGGAAAGACTATCTATGGTGAAGGACACAGAAGGCAAAGAAATATCGGAGCACAAAAGCTGGCTGAACGCATTCTTATCTATGTATCGTACAATAGAGAG
GTCGCCTTATGGCTTGAGAACCTGCAGAGAAGGAAGGAACGTTATTACAGGGAGAATCTGGAGGTAATTCTACGCATAATTCCCGGATATGACAAAGCCA
TCCTGACAGAAGCGGTTAGTGTATGTCTGGACAAGGGAATCTATAATGGTGAGTCCGTTAAAAGCCTGTGCGGACATATATGGAAGAAGAAAATGAGAGA
ATCGGATGTAGGAAAAAATCCTGCTTCCCGGACACAGTCAACCGGATTGGTAAAAACATATAATGAAATCTTTAGAAACAATGGCAAGGTATAAGAAAGA
ACTGACCGAATGTGCCCGCAACCTGAAGCTGCCATTCCTGGCAGAACACCTGGATGAAATACTACATGAAGCACAGGAAAAGCAACAGACTTACTCCGAG
TTTCTGTCAACTTGTCTGATGCGGGAACTTCGGGACAAGGAAAGGAGAAGTTATCTGACCAGGTTGAAATTTGCAGGATTGCCTGCAAGGTATGATCTGG
ATCTATATGATTTCTCACGTACCGAAGGGATTGACCAAAGGCAGATGCGCGAATTGCGTGAACTGGTATGGATAAGAAGGACATATAATCTTCTGCTGGT
AGGAGATTCCGGAACCGGAAAGACATTCATTGCTTCAGGACTTATCCATGAAGCAGTGAAAGCGGGTTATAAGGCATACCTGCTGACCTTGGAAGAACTG
TTTGTCTGTTTGAAGACTAAGGAGATATCACGACCCGCGATGAAAACATACAAACGAATAATGAAAGCGCAGCTGCTGGCAATCGATGATGTTACGCTGT
TTCCCCTGAAAGGAGAAGATGTACTGCTACTGTTTAAACTGGTGAATTGCGTTCAAGGTAAGACATCACTTATCATTACCGCAAGCCGGGATCTTACCGG
ATGGCTGGAGATGGCAGGAGACGAAGTTTGTGCGGCAGCTCTACTGGACAGACTACTCTATTGTTGTGAGATAATCAGGCTATCAGGAAAAAGCTACCGC
ATGGAAAACAGGAAAACAATTTTTAGCAATCAACAGATAGGGACTGCATCTTAAAAAAAAGTTAATGAAAGTAAAGAAGAGAACTAAGGAAAGTGGATAC
TGTTAAATTGCACAAAAAAGGGACCTGTAAATTTGCTCGGTACA
Protein section
ORF number : 2

 

ORF 1
LengthBeginEndStrandFusion ORF
1560 bp519 aa1351694+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MEIRKQYFKEKQYMWHKVRELQLKGLNKTQIGIYLGVNRKTVRRYLNMTMEEFVKKQSSHRKYRLKLENYEQYVRANLEEYPYISAARIHDWLKECYPDF
PRVCNRTISGFVERVRKKYGIGKKVETHKRNYEKQPDTPYGEYAQADFGEKCMRTENGKSIKVYFFAIVLSRSRYKFIYFSRRPFDTGLAVYAHELAFEY
FGGRPQKIIYDQDKVLIARANMGDLILTGKFQAFVKEQHFHPVFCHKADPESKGRVENVVKYVKTNFLTARIFQNVDRLNEEARLWLERTGNGKEYGTTH
RIPLEEFAQEREYLVPYHGTPHSPGGEMKEYHVRKDNTVQYRGNYYSLPCGTYRGGETTVWLHETDGCLELYNKETGKLVCRHDLCELKGKTIYGEGHRR
QRNIGAQKLAERILIYVSYNREVALWLENLQRRKERYYRENLEVILRIIPGYDKAILTEAVSVCLDKGIYNGESVKSLCGHIWKKKMRESDVGKNPASRT
QSTGLVKTYNEIFRNNGKV

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
792 bp263 aa16632454+No
ORF function : Accessory Gene
AG : IS21 helper

ORF sequence :

MKSLETMARYKKELTECARNLKLPFLAEHLDEILHEAQEKQQTYSEFLSTCLMRELRDKERRSYLTRLKFAGLPARYDLDLYDFSRTEGIDQRQMRELRE
LVWIRRTYNLLLVGDSGTGKTFIASGLIHEAVKAGYKAYLLTLEELFVCLKTKEISRPAMKTYKRIMKAQLLAIDDVTLFPLKGEDVLLLFKLVNCVQGK
TSLIITASRDLTGWLEMAGDEVCAAALLDRLLYCCEIIRLSGKSYRMENRKTIFSNQQIGTAS

 

Blast result :
Comments
The first founded ISBf2 sequence (U05886) is located 50 bp upstream the translation start codon of cepA, a chromosomal cephalosporinase gene, whose high expression level is apparently driven by an ISBf2 outward promoter.
June 20 2012 : This sequence will be replaced by the most closely related (98% DNA identity).
References
1] Rogers, M.B., Bennette, T.K., Payne, C.M., and Smith, C.J. (1994) J. Bacteriol. 176, 4376-4384.
2] Kuwahara,T., Yamashita,A., Hirakawa,H., Nakayama,H., Toh,H., Okada,N., Kuhara,S., Hattori,M., Hayashi,T. and Ohnishi,Y. (2004) Proc. Natl. Acad. Sci. U.S.A. 101 (41), 14919-14924