ISPsy43

  • Family IS66
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
CP012179 ND Pseudomonas syringae
Pseudomonas syringae pv. actinidiae
DNA section
IS Length : 4875 bp

Ends


IR Length : 17

IRL : GTAACCGTCCGGGCAACACACCTTCCTGACACCGTCGCTTGAGGCGATGG
IRR : CGTAACCGTCCGGGCAACTCATCTAGCGTAGCTGGCTCTATTGTTTTACG

Insertion site


Left flankDirect repeatRight flankDR Length
GTCATAGCAAGCTCTTCGACAAGCCAGT8

DNA sequence

GTAACCGTCCGGGCAACACACCTTCCTGACACCGTCGCTTGAGGCGATGGTGTCCACCCAAATATAGGTGGACACCATTTTTAGCCTTTTTAAGCGGACG
CCCATGCCACAAGAACGCCGTTCCTATTCCAAGTCCTTCAAGGCCCAGGTCATTGCTGAGTGCGCCCAACCTGACACTTCAATTGCCAATGTCGCCTTGA
CCCACAACCTCAATGCAAACCTCGTCCATAAATGGATTCGGGTGCATGCACAGAAAAACCTGACACTTCAAACCGCCTTCATCCCGGTCAAGACATCGCC
GCCGGTGCCGATGCATCAGGCCCTTCCTGCCACGATCCGAATCGAAGTGCCGCATTCAAAAGGCGTAGTCGTGGTGAGTTGGCCGGCAGAAAATGCAGCG
GCGTGTTCTGCTTTCCTGCGAGACCTGCTGCGATGATCCGCATCGACTCCATCTGGCTTGCCACCGAGCCCATGGACATGCGCGCAGGCACTGAAACTGC
GCTGGCGCGGGTCGTGGCGGTGTTCGGTGCGGCGAAGCCGCACTGTGCTTATCTGTTTGCCAACCGTCGTGCCAATCGCATGAAAGTGCTGGTGCATGAT
GGCTTGGGGATTTGGCTTGCTGCCCGGCGCTTGAATCAAGGACGTTTTTTTTGGCCGGGCGTGCGGCACGGCTCTGAAGTCGAGTTAGATGCAGAGCAAC
TTCAAGCCTTAGTGCTCGGTTTACCTTGGCAACGCGTCGGTTCCGGAGGAGCCATCACGGTGCTCTAACTCGTCTGCCATGGCGCCCTTGCCAGCGCAAT
TGGCCCATCAGTCTATCGCCGCCACTAGCCCTCTCTGGCAAAATCGGCGGCATGACTTCGCATCCGAATCTCGATCAATTAAACCCTGAAGAACTGCGTG
CCTTGGCGGCGCAGTTGATCCAGCGCGTCGAGACGATGGACAAGCAAATCACTCATCACAAGTCGGTCAACGAGAAACTGGCCCACGAGATCGCGCTGCT
CAAACGCTTCAAGTTTGCCAAGCGCAGCGAGCAGTTGAGCCCGGATCAAGCCAGCCTGCTCGACGACTTGATCGATACGGATATCGCCGCTATCGAAGCC
GAGCTTGAGGCGCTGCTACCGGCACCTGTCGAAGCCAAAGTGCGCCAGCAACCCAAGCGCGCACCGCTGCCACCGCAGTTTCCTCGCACACTGATCCATC
ACGAGCCTGACAATAGCCACTGCCAGTGCGGCTGCGCCCTCAAGCGCATCGGCGAAGATGCCAGCGAAAAGCTCGACTACACGCCCGGCGTGTTCACCGT
CGAGCGCCACATCCGTGGCAAGTGGGTCTGCGAACAGTGCGAAACGCTGATACAGGCGCCGGTACCGGCGCACGTCATCGATAAAGGCATCCCGACAGCG
GGCCTGCTGGCCCACATCATGGTGGCCAAGTTTGCCGACCATCTGCCGCTGTATCGCCAAGAGAAAATCTTCGGCCGCGCCGGGCTGCCCATCGCCCGTT
CGACCTTGGCGCAGTGGGTGGGCAACTGCGGCGTGCAATTACAGCCGCTGGTTGATGCGCTGCGCGAAGCCGTGCTGACGCACGACGTCGTTCACGCCGA
CGAGACGCCGGTACAAATGCTGACGCCTGGTGCGAAAAAAACTCATCGCGCTTACGTCTGGGCCTATGCCACCAGTCAGTTCTCCAACCTGGCGGCGGTC
GTTTACGACTTCAGCCCGAGCCGCGCTGGCGAACATGCCCGAGCCTTCTTGGGCAGTTGGAACGGCAAGCTGGTCTGCGATGACTTTGCCGGCTACAAGG
CAGGCTTTGAACTGGGCGTTACGGAGATCGGCTGCATGGCCCATGCCCGCCGGAAGTTCTTCGACTTGCACGCGACCAACAAGAGCCAGGTCGCCGAAAA
AGCGCTGAACTACATCGCGGCCTTGTACGAAGCTGAACGAGAAGTCCGAGAGCTGGAGCCGGGTGATCGGCAGCGAATACGGCAGGAAAAAGCAGCGCCA
ATCGCCGACGCACTTCATACCTGGATGATCGCCCAAAGACAGCTTGTGCCTGAGGGTTCGGCCATCGCCAAGGCGCTGGATTACAGTCTCAAACGCTGGA
TAGCGCTGACGCGCTATCTCGATGACGGTGCCGTGCCCATCGATAATAACTGGTGCGAGAATCAAATCCGGCCGTGGGCTCTTGGGCGTTCGAACTGGCT
GTTCGCGGGATCGTTACGCAGCGGTAAACGTGCAGCGGCGATCATGAGTTTGATCCAATCGGCGCGACTCAACGGCCATGATCCGTATGCTTATTTGAAG
GACGTACTCACGCGCCTGCCGACGCAGCGGGCAAGTGAGATTGCGGAGCTACTACCGCATAGGTGGCGACCGGTTTAGTTGCGCAAGACGGGATGCCCGG
ACGCACCTCCAACTGGTGCCATTACCGGTCTACTTAGCCTTTATGCTGGTTATCCATGCATTCCTCCCTACAGCTTCGCCCGGTTCAGCCGATAGTTTTC
GTACCAAGAGAAACGCGGCAATTCGATCCTGTAACAAAGGAGAGTAAGCGGCTTTTGAGGAAGATCTCTTGGAATGCTTTCATTACCTCATACCCATAAA
TGAGAGATGGGCACTTTTTAACGCCTAAGAAAGCACTCACAGACCTTGAGTGATCGAGAACCCGATGAATTTATTAGCGCCTGCCCTGTTCGTTACCAAC
CGTGTTCGATTCCCCATGAAATTCGCAATTTTGGGATTTATTGTCCTGATTCCTCTGCTCCTGCTAGGCACCCGGGCGATGCTTTCACTGAACACGAGCA
TCACGGGCATCAAGCATGAACAGGTGGGTCAACAATACTTGTTGGACGTCACCCCTATCCTGCGTCTAACCATGATTCAGAGGGCGCTGACACACGGCAT
GCTCAGCGGTGACACCAACGCAGTAGCCAATGCAGCACGCAATGCTGAAAAGCTTAACGATGCCTATGCGACTCTTGCTGCGCAGGACGCAAAATTTAGC
ACCCAACTGGCAACGACAGACCGAGTTCAGACGTTGCGTACCGCTAGTGTGCAGCTGGTTGAACGCGCCAAAGCAGGCGAAGCACCTTTGGTGATCTTTT
CCGCGTGGAACGACCAGTTAACCGACCTAATGAATTTCGTTTACTACATTACGGCCACTTCCGGCATGATTTTGGACGAAGATGCAGGTTCGTTGTACCT
CATCGATCTAAGCTCTATACGTCTGCCTCGGCAAATAAATCTGGTCGGACAGATTCGCGGTCTGGCCAGCGGTTTTAGCGCTGACCGTCCTCTGGACGAC
ACCACTCGTATTTTTGCCCAGACTTTGTTGAAACAGGAGCTGCTAATGCGTCAAGAGTTGCAGCAGAGCCTTTCCCTGCTTCGCCGAGAAGAGCCGAAAT
TATCTGGTGTAGTTCAGCCGTCTGTAACAACAGCCATTACCGACCTGGAAAATCTGCGTAAAGACCTGGTGGACTACCTTGATCCGAGCAAGCGCTCAAA
CGTGAATGCCAACACCTTGGGCGAGCGTGGAAACGCCGTCGTTGCTCAGTTTTACAAAGCTCAGGATCAGATGCAGGAATCGCTGCGAATTCGAATTGAT
GAGCGTCTGAATACACTGAGTAATGAGCGTACTTTCGGCACTGAGCTATTTATCGCCATTGCGATACTGCTGCTCTATGCGTTCGTCGGCATCTACAAGG
CTCTGCGGATTGGGGTTGATGAGCTCCTAGCTGTGACGGCACGTATTGCTCAAGGCGATCTGAGCGCGCGCGTCCGAATCAACAGCCACGATGAAATCGG
TGATGTAGGCGAGGGACTTAACCGCATGGTAACGGCCTTCTCTGGCTCACTGAGCCAGGTGGAGCGTAGCTCGCATTCAGTCTCAGATGCGGCAAAGCGA
CTAGAGATCTCCATAGGCCAAGCCAAAGCCTCAATGAACTCACAACAGGCTGAAACCGAGCAGGTTGCTACTGCCATCAACGAAATGACCGCGAGCGTTG
CCGACGTGGCCCAGAATACCGAGGGCGCAGCACGTGCCGCTGAATCTGCAAGCAGTGCGTCTAACGACGGCTTGAAAGTTATGATTGAAACAAGTGCCGC
TATTGAGGCGTTGGCCAACGAAGTGGAGCTCAGTGCAAGCAAAGTCGAAGCGTTAGCGACACACAGCAAAGAAATCGGTGGTGTCATCGAGGTCATCAGT
ACCATCGCGGATCAGACTAACCTGCTGGCCCTTAACGCGGCCATCGAGGCGGCGCGAGCTGGCGAGCAAGGCCGTGGTTTCGCGGTCGTGGCTGACGAAG
TGCGGACTCTGGCCTCGCGCACACAAAACTCCACAGAAGAAATTCGACGGATCATCCAACAGTTGCAAGGCGCTACTGATGCCGCTGTTCAACAGATGAA
AGCTGGGCAAACCCGAGCTAGGGAATGCATTCAGGCAGCGAGCCAAGCGTCTGGCAGCCTGAACCAGATCAATGAAGCAGTAGACGGAATTGTGGGCATG
AACACTCAGATTGCAAGTGCGGCGGTGCAGCAACATTCGGTCTCAGAGGATATCAATCGCAACGTCATCGGCATTCGAAACAGCAGCTTAATCGTCATGG
AAGGCGTAGAAGACAACGCCGTAACGGCTGACGAGCTTTCATTGCTCGCTAATGAACTTCGCAGCGTAGTCGGCAAGTTTAAGTTATCCAGCAACGCTTG
AAAGGAATCTCAAGCGTTTCAATTGTTGATAGCACGAGAAAGTGCTTTGATCGGGTCTGACTGGGGTGATGATGCCCGGCAGTCCCTGATCGACATTGAG
GGCTGAGCTTCATCGTTGCGCAATCTCGTAAAACAATAGAGCCAGCTACGCTAGATGAGTTGCCCGGACGGTTACG
Protein section
ORF number : 3

 

ORF 1
LengthBeginEndStrandFusion ORF
537 bp178 aa232768+No
ORF function : Accessory Gene
AG : IS66 TnpB

ORF sequence :

MDSGACTEKPDTSNRLHPGQDIAAGADASGPSCHDPNRSAAFKRRSRGELAGRKCSGVFCFPARPAAMIRIDSIWLATEPMDMRAGTETALARVVAVFGA
AKPHCAYLFANRRANRMKVLVHDGLGIWLAARRLNQGRFFWPGVRHGSEVELDAEQLQALVLGLPWQRVGSGGAITVL

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
1527 bp508 aa8522378+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MTSHPNLDQLNPEELRALAAQLIQRVETMDKQITHHKSVNEKLAHEIALLKRFKFAKRSEQLSPDQASLLDDLIDTDIAAIEAELEALLPAPVEAKVRQQ
PKRAPLPPQFPRTLIHHEPDNSHCQCGCALKRIGEDASEKLDYTPGVFTVERHIRGKWVCEQCETLIQAPVPAHVIDKGIPTAGLLAHIMVAKFADHLPL
YRQEKIFGRAGLPIARSTLAQWVGNCGVQLQPLVDALREAVLTHDVVHADETPVQMLTPGAKKTHRAYVWAYATSQFSNLAAVVYDFSPSRAGEHARAFL
GSWNGKLVCDDFAGYKAGFELGVTEIGCMAHARRKFFDLHATNKSQVAEKALNYIAALYEAEREVRELEPGDRQRIRQEKAAPIADALHTWMIAQRQLVP
EGSAIAKALDYSLKRWIALTRYLDDGAVPIDNNWCENQIRPWALGRSNWLFAGSLRSGKRAAAIMSLIQSARLNGHDPYAYLKDVLTRLPTQRASEIAEL
LPHRWRPV

 

Blast result :
ORF 3
LengthBeginEndStrandFusion ORF
2034 bp678 aa26654698+No
ORF function : Passenger Gene
Annotation : methyl-accepting chemotaxis proteinDescription :

ORF sequence :

MNLLAPALFVTNRVRFPMKFAILGFIVLIPLLLLGTRAMLSLNTSITGIKHEQVGQQYLLDVTPILRLTMIQRALTHGMLSGDTNAVANAARNAEKLNDA
YATLAAQDAKFSTQLATTDRVQTLRTASVQLVERAKAGEAPLVIFSAWNDQLTDLMNFVYYITATSGMILDEDAGSLYLIDLSSIRLPRQINLVGQIRGL
ASGFSADRPLDDTTRIFAQTLLKQELLMRQELQQSLSLLRREEPKLSGVVQPSVTTAITDLENLRKDLVDYLDPSKRSNVNANTLGERGNAVVAQFYKAQ
DQMQESLRIRIDERLNTLSNERTFGTELFIAIAILLLYAFVGIYKALRIGVDELLAVTARIAQGDLSARVRINSHDEIGDVGEGLNRMVTAFSGSLSQVE
RSSHSVSDAAKRLEISIGQAKASMNSQQAETEQVATAINEMTASVADVAQNTEGAARAAESASSASNDGLKVMIETSAAIEALANEVELSASKVEALATH
SKEIGGVIEVISTIADQTNLLALNAAIEAARAGEQGRGFAVVADEVRTLASRTQNSTEEIRRIIQQLQGATDAAVQQMKAGQTRARECIQAASQASGSLN
QINEAVDGIVGMNTQIASAAVQQHSVSEDINRNVIGIRNSSLIVMEGVEDNAVTADELSLLANELRSVVGKFKLSSNA

 

Blast result :
Comments
ISPsy43 is also known as Tn6211.
ISPsy43 is 95% (orfA) and 88% (orfB, the transposase) aa similar to ISPpu19
References
1] Butler M. (2017) Direct submission to ISfinder
2] Butler et al., 2013, PLoS ONE, 8(2): e57464, Epub 2013 Feb 27