ISVa18
- Family IS4
- Group IS50
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| WP_053411098 | ND | Vibrio anguillarum | Vibrio anguillarum serovar O2 VIB12 Vibrio hepatarius |
DNA section
IS Length : 1504 bp
Ends
IR Length : 16/21
IRL : cctgtctcttatacacaaatccccaagcctctgcttggggatttgtttga
IRR : ctgtctcttgatcacaagtctggtgaatcaagagacttagccagttcata
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| aataaaggaa | gcgttggg | cttcctttat | 8 |
| caaaaaggaa | ggtttgtc | cttccttttt | 8 |
| caaaagggct | ggcttatggc | caaccctttt | 10 |
| caaaaagctt | ggtgatgag | catccaatgt | 9 |
| acc | accctagc | cctccccttg | 8 |
| agaaagggat | gcctcag | catccctttg | 7 |
| aaaaagggaa | gcctaag | cttccctttt | 7 |
DNA sequence
cctgtctcttatacacaaatccccaagcctctgcttggggatttgtttgaactaattttatgtttcatgatctgatcatctaagcaataacaaggatgat
tagaatgacttatatagaaccaaccctctgggcacaaaagcaatttggtcaagctcacctcaatgacccaagacgtactcaaagactcgttgccctagca
gcctctttggccgagcaacctggcgtaccggtctcgaaactcatcatctcccctgcagatatggaaggggcttatcgctttattcgtaatgagcaaatca
aagcagaagatatcgctgaagcgggcttttatgtcacagcgcaagaggctttagaacaacaaacacttcttgcattagaagatacgacttctctaagcta
ctcccatcgcagcatacaagatgaacttggacactccaatcaaggcaatcgaaatcgagctatgttcatacactcgaccttactttttgctcccgaaact
caagtggttgttggcttaattgaacaacaacgctggactcgagatattgaaaagcgtggacaaggacaccagtacgccactcgaccatacaaagagaaag
aaagctataaatgggagcaagcttctcgccatgtcgcagagcgacttggcgagaaaatctcagatgttatttctgtgtgcgatagagaagccgacctatt
tgaatacctcacttacaagcaagagcaacaacagcgattcctcgttcgctcaatgcaaagtcgctgtataaaagagcatgataatcgtctttatgactac
gcttccaagctgcaatcagcaggagaaagagtgctcgacataccgcaaaaaggcggccgtaaagctcgcacggttcacctagacatcaaatatgcccccg
tgacactcaaatctcccgcgaataaaaaagagttcagtaacattcctctttactacgtgggatgcatagaacaaggagagagtaacgataagctcgcatg
gcacttactgacctcagagccagtaacgagcagggaagaggcacttaaaatcgtcagttattatgagctgcgctggctaattgaagattttcataaagtc
tggaaaagtgaaggtactcaagtcgagcaattgagaatgcaaagcaaagataacttagaaaggctcagcgttattctggcttttatcgcgactcggttgc
tccagttgagatttatgaatgaatcagatgagttatctaagagcagttgtgagccgatattaaaaggcaaagcatggaagctaatgtggctaaagctaga
aagaaaagggctaccgaaggaagctccggacatatcatgggcttacagaggtattgctcgattagggggttggaagaatacaaagcgaacgggtcgtgct
tctataaagacattatggcaaggatggtttaggttacaaaccatccttgaagggtatgaactggctaagtctcttgattcaccagacttgtgatcaagag
acag
tagaatgacttatatagaaccaaccctctgggcacaaaagcaatttggtcaagctcacctcaatgacccaagacgtactcaaagactcgttgccctagca
gcctctttggccgagcaacctggcgtaccggtctcgaaactcatcatctcccctgcagatatggaaggggcttatcgctttattcgtaatgagcaaatca
aagcagaagatatcgctgaagcgggcttttatgtcacagcgcaagaggctttagaacaacaaacacttcttgcattagaagatacgacttctctaagcta
ctcccatcgcagcatacaagatgaacttggacactccaatcaaggcaatcgaaatcgagctatgttcatacactcgaccttactttttgctcccgaaact
caagtggttgttggcttaattgaacaacaacgctggactcgagatattgaaaagcgtggacaaggacaccagtacgccactcgaccatacaaagagaaag
aaagctataaatgggagcaagcttctcgccatgtcgcagagcgacttggcgagaaaatctcagatgttatttctgtgtgcgatagagaagccgacctatt
tgaatacctcacttacaagcaagagcaacaacagcgattcctcgttcgctcaatgcaaagtcgctgtataaaagagcatgataatcgtctttatgactac
gcttccaagctgcaatcagcaggagaaagagtgctcgacataccgcaaaaaggcggccgtaaagctcgcacggttcacctagacatcaaatatgcccccg
tgacactcaaatctcccgcgaataaaaaagagttcagtaacattcctctttactacgtgggatgcatagaacaaggagagagtaacgataagctcgcatg
gcacttactgacctcagagccagtaacgagcagggaagaggcacttaaaatcgtcagttattatgagctgcgctggctaattgaagattttcataaagtc
tggaaaagtgaaggtactcaagtcgagcaattgagaatgcaaagcaaagataacttagaaaggctcagcgttattctggcttttatcgcgactcggttgc
tccagttgagatttatgaatgaatcagatgagttatctaagagcagttgtgagccgatattaaaaggcaaagcatggaagctaatgtggctaaagctaga
aagaaaagggctaccgaaggaagctccggacatatcatgggcttacagaggtattgctcgattagggggttggaagaatacaaagcgaacgggtcgtgct
tctataaagacattatggcaaggatggtttaggttacaaaccatccttgaagggtatgaactggctaagtctcttgattcaccagacttgtgatcaagag
acag
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1389 bp | 462 aa | 105 | 1493 | + | No |
Chemistry : DDE
ORF sequence :
MTYIEPTLWAQKQFGQAHLNDPRRTQRLVALAASLAEQPGVPVSKLIISPADMEGAYRFIRNEQIKAEDIAEAGFYVTAQEALEQQTLLALEDTTSLSYS
HRSIQDELGHSNQGNRNRAMFIHSTLLFAPETQVVVGLIEQQRWTRDIEKRGQGHQYATRPYKEKESYKWEQASRHVAERLGEKISDVISVCDREADLFE
YLTYKQEQQQRFLVRSMQSRCIKEHDNRLYDYASKLQSAGERVLDIPQKGGRKARTVHLDIKYAPVTLKSPANKKEFNNIPLYYVGCIEQGESNDKLAWH
LLTSEPVTSREEALKIVSYYEQRWLIEDFHKVWKSEGTQVEQLRMQSKDNLERLSVILAFIATRLLQLRFMNESDELSKSSCEPILKGKAWKLMWLKLER
KGLPKEAPDISWAYRGIARLGGWKNTKRTGRASIKTLWQGWFRLQTILEGYELAKSLDSPDL
HRSIQDELGHSNQGNRNRAMFIHSTLLFAPETQVVVGLIEQQRWTRDIEKRGQGHQYATRPYKEKESYKWEQASRHVAERLGEKISDVISVCDREADLFE
YLTYKQEQQQRFLVRSMQSRCIKEHDNRLYDYASKLQSAGERVLDIPQKGGRKARTVHLDIKYAPVTLKSPANKKEFNNIPLYYVGCIEQGESNDKLAWH
LLTSEPVTSREEALKIVSYYEQRWLIEDFHKVWKSEGTQVEQLRMQSKDNLERLSVILAFIATRLLQLRFMNESDELSKSSCEPILKGKAWKLMWLKLER
KGLPKEAPDISWAYRGIARLGGWKNTKRTGRASIKTLWQGWFRLQTILEGYELAKSLDSPDL
Blast result :Comments : Transposase, common in Vibrios
Comments
ISVa18 is 83% aa similar to ISUnCu8.
Nucleotide variations in positions: 45 (k: g or t), 937 (r: g or a), 947 (k), 971 (r: g or a), 1069 (w: a or t) and 1115 (y: t or c).
Nucleotide variations in positions: 45 (k: g or t), 937 (r: g or a), 947 (k), 971 (r: g or a), 1069 (w: a or t) and 1115 (y: t or c).
References
1] Holm K.O. (2016) Direct submission
2] Busschaert P. et al (2014) Journal of Fish Diseases (doi:10.1111/jfd.12290)
3] VIB12 in Austin B. et al (1995) Systematic and Applied Microbiology 18: 285-302
2] Busschaert P. et al (2014) Journal of Fish Diseases (doi:10.1111/jfd.12290)
3] VIB12 in Austin B. et al (1995) Systematic and Applied Microbiology 18: 285-302