ISAni1

  • Family IS481
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
X97015 ND Arthrobacter nicotinovorans
Arthrobacter nicotinovorans plasmid pAO1
DNA section
IS Length : 1075 bp

Ends


IR Length : 23/27

IRL : TGTATTGACCACGGACGTTAGTGACGCTCGGCGTGGTTTGGTGACATGAA
IRR : TGTATTGACCTGAGACGTTGGTGACGCGGCTGGCTGGCGTCTGACCTTTG

Insertion site


Left flankDirect repeatRight flankDR Length
TGTGGATCTTACTAGTTGCTGCCGGG6

DNA sequence

TGTATTGACCACGGACGTTAGTGACGCTCGGCGTGGTTTGGTGACATGAAGAAGACCTCCGGGTGGAGTGGGGCTTGTCTAGAGTCCTTTTCCACACACG
GAGGTCTTCGTGTCCCACCCTAACGCTTTCCTCACTCCTCGTGGCCGGTTGCAGCTCGCGCAGTGCGTTGTTGATCAGTGCTGGAGCCTGCGCCGTGCCG
GAGCGGTTTCAGGGGTCTCGATCCCGACCGCTGAACGTTGGGCGAAGCGCTACCTCGAGGAGGGACCCGAAGGAATGGAGGACCGTTCGAGTCGGCCACA
TTCCTCGCCCCGCGGGACTGCTACGCGTACCGAGCGGAGGGTCATCGCCGTGCGCGTGAACCGCCGGTGGGGCCCTGCCAGGATCGCGGCTATTTGCTGG
GCCTGCACCCCTCAACCGTCCACCGGGTACTGTCCCGCCACCGGCTGGCGAAACTAACCTGGCTCGATCGCGGCACAGGACGAGTGATCCATCGCTATGA
ACACGACAGGCCCGGGGATTTGGTCCACCTGGACATCAAGAAGCTGGGCCGGATCCCCGACGGCGGCGGACACCGAGTCATGGGCAGGACCGCCGGCTGG
AAGAACAAGACCGGCACCAAGACCAACCGCCGGCCTGGCTACCACTACCTCCACAATGCCGAGGATGACCACTCACGACTGGCTTACACCGAGATCCTCA
CTGACGAAACCAAGGAAACAGCGGCAGACTTCTGGGTGCGGGCAAACGCCTGGTTCAAAGAACACGGAGTTACCGTCCAGCGTGTTCTGGCTGACAACGG
GAACTGCTACCGGTCTCATGCTTACGCCGAAGCGCTAGGGCCGGACATGAAACACAAACGCACCCGCCCCTACAGGCCACAAACCAACGGCAAAGTCGAA
CGGTCCAACGGCACCATGCTCGAGGAATGGGCCTACATCCGCCCCTACCGCTCAGAAGCCGAGCGGGTTGCTGCTTTCCCCGACTGGCTCCATGCCTACA
ATCACCATCGAGCCAACACTGCGCTCAAAGGTCAGACGCCAGCCAGCCGCGTCACCAACGTCTCAGGTCAATACA
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
971 bp323 aa1101080+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MSHPNAFLTPRGRLQLAQCVVDQCWSLRRAGAVSGVSIPTAERWAKRYLEEGPEGMEDRSSRPHSSPRGTATRTERRVIAVRVNRRWGPCQDRGYLLGLH
PSTVHRVLSRHRLAKLTWLDRGTGRVIHRYEHDRPGDLVHLDIKKLGRIPDGGGHRVMGRTAGWKNKTGTKTNRRPGYHYLHNAEDDHSRLAYTEILTDE
TKETAADFWVRANAWFKEHGVTVQRVLADNGNCYRSHAYAEALGPDMKHKRTRPYRPQTNGKVERSNGTMLEEWAYIRPYRSEAERVAAFPDWLHAYNHH
RANTALKGQTPASRVTNVSGQYN

 

Blast result :
Comments
ISAni1 is 63% similar to IS1652 and IS1649, 64% to IS5564, 61% to ISSco1and 57% to U85507.
There is a -1 frameshift at position 375 which has been "corrected" in the translation of the transposase.
Note that the transposase termination codon is generated by sequence specific insertion.
References
1] Menendez,C.U. (1996) Direct Submission GenBank.