ISAbc1

  • Family IS1595
  • Group ISSod11
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
MNKO01000038 ND Acidobacteria bacterium
Acidobacteria bacterium 13_1_20CM_2_57_8
DNA section
IS Length : 1056 bp

Ends


IR Length : 30/34

IRL : GTTGGCTTATGCTTATACGGTTGTTTCAGCAAACCACGACCGGTAGTATG
IRR : GTCGCCTTCTGCTTATACGGTTGTTTCAGCAGACCCCAATAGATTGCCGC

Insertion site


Left flankDirect repeatRight flankDR Length
GCGAACACGATAATAGAGCGAGGGT0

DNA sequence

GTTGGCTTATGCTTATACGGTTGTTTCAGCAAACCACGACCGGTAGTATGAGCACGTGTCTCGCCCGCCGTTTCCGAAGACGTTGCGCGAATTCCAGACG
AAGTTCGCCAGCGATGAAGCGTGCCAGCAATACCTGGCCGCGTGTCGCTGGCCCGATGGCTTCGTTTGTCCACGATGCGGACAACGGCGAGCATACGAGT
TGGTGCAGCTGCGGCGTTGGCAGTGCGCCGGTTGTCGGCATCAAGTTTCGCTGACGGCAGGAACGATCCTGCATAACACGAAAATTGCGCTGACGGTTTG
GTTCTGGGCCGCCTACCTGATGACGACGGATAAGCGCGGCCTCTCCGCCCTGCTCTTGCAACGGCAGCTTGCCTTGGGACGTTATGAGACGGCCTGGATG
ATGCTCCATAAATTCCGGCGCGCAATGGTCAATCTGGTACGGGAGCCACTGCAAGGCGAAGTGGAAGTTGATGATACGTGGATTGGAGGTGAGCAGGCAG
GCTTGCGCGGGAGCCGGCAACTGAAAGGTCGGAAAGCGGCGCTCGTCCTTGTTGCAGTGGAGAAACGAGGCCGCGCAACCGGCCGCGCTCGGATGGCCGT
CATCCCGGATTTCAAGAGCGCCACTCTACTCGCTTTCCTCAAACAAAACGTGGCACCAGGTGCAACCGTATACACCGATGGCCTCAAAAGCTTCACGGGA
TTACAGGAAGCTGGATTCGAGCACGTGCCTCGAATTCAACCGCTGCGTATTGATCTGCGTAAAGGCGCAAAATCCGTGGTGCCCTTAGCTGACCGCGCAA
TTGGCAATCTGCAACAATGGCTGATCGGAACCCATCACGGCGTGAGCCGCGGTCAGCTTCCGGTTTATCTCGACGAATTCGTCTTCCGGCATAATCGCCG
AAAGCACCCCATGGCTGCATTCCAGACCTTGCTCGGTCTGGGAACCGCGCGCAATCCAACGCCTTACGATAAAATACGCGGCGCGGCTGATCTTTCTGCA
AACTAAGCGGCAATCTATTGGGGTCTGCTGAAACAACCGTATAAGCAGAAGGCGAC
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
951 bp316 aa561006+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MSRPPFPKTLREFQTKFASDEACQQYLAACRWPDGFVCPRCGQRRAYELVQLRRWQCAGCRHQVSLTAGTILHNTKIALTVWFWAAYLMTTDKRGLSALL
LQRQLALGRYETAWMMLHKFRRAMVNLVREPLQGEVEVDDTWIGGEQAGLRGSRQLKGRKAALVLVAVEKRGRATGRARMAVIPDFKSATLLAFLKQNVA
PGATVYTDGLKSFTGLQEAGFEHVPRIQPLRIDLRKGAKSVVPLADRAIGNLQQWLIGTHHGVSRGQLPVYLDEFVFRHNRRKHPMAAFQTLLGLGTARN
PTPYDKIRGAADLSAN

 

Blast result :
Comments
ISAbc1 is 65% aa similar to ISRop3.
References
1] ISfinder annotation (2017)
2] Butterfield,C.N., Li,Z., Andeer,P.F., Spaulding,S., Thomas,B.C., Singh,A., Hettich,R.L., Suttle,K.B., Probst,A.J., Tringe,S.G., Northen,T., Pan,C. and Banfield,J.F. ( 2016) PeerJ 4, E2687.