ISPsy40

  • Family IS21
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
CP012179 ND Pseudomonas syringae
Pseudomonas syringae pv. actinidiae
DNA section
IS Length : 2644 bp

Ends


IR Length : 24

IRL : TGGGGATTCCACGTGCACTTGGACACCCATTCCACGAATACTTGGACAGT
IRR : TGCGGATTCCACGACACTTGGACACTCAGCCCACGATCATTTGGACACTC

Insertion site


Left flankDirect repeatRight flankDR Length

DNA sequence

TGGGGATTCCACGTGCACTTGGACACCCATTCCACGAATACTTGGACAGTGATTCCACGCTGACTTGGACACTCATTCCACGGCAACTTGGACACTGATT
CCACGAGCATCTGGACAGTGATTTCTCACTGACCAGCCCCAAGCTCAGGCAGGCAGCAACGCAGGATTATTCACTACCATCGACCTCTTTTTTCGAAGCA
AAGGGGTCGTCGTGGAGCGTTTATCCATGCGTAAAGTCCGAGAGGTACTACGCCTAAAGTTCGACGCCGGCCTGTCCGTGCGCAAAATCGCCGCCAGCCT
ACGCATCAGCAGCGGCAGTGCCGGCAATTACCTGCATCGGTTCAACGCCTGCGGTTTGAGTTGGCCTACGTCATTGTCCGATGCCGAGCTGGAGCGGCTT
TTATTCCCACCAGCTCCAACCGTGCCAAGCGATCAAAGGCCGATGCCTGACTGGGCGTGGGTACACGCTGAGTCGCGCCGTCCCGGCGTCACCCTGGCGC
TGCTCTGGCAGGAATATCGACTCGCCCACCCGCAAGGCTTTCAATACAGCTGGTTCTGCGAGCACTACCGCCTCTGGGCCGCCAAAGTCGATGTGGTCAT
GCGCCAGGAACACCGTGCCGGCGAAAAGCTGTTCGTCGATTACGCAGGACAGACCGCCCCAATCATCGACAGGAGCACTGGGGAGATCCGTCAGGCTCAG
ATCTTCGTCGCTGTCCTCGGCGCATCCAGTTACACCTTCGCCGAGGCCACCTGGTCGCAGAAACTGCCCGACTGGCTGGGCTCGCACGTCCGATGCTTTG
CTTTTTTCGGCGGCACAACGCAGATCCTGGTGCCCGATAATCTGCGCAGTGGCGTGACTAAAGCGCACCGTTACGAGCCGGATATCAACCCCAGCTACCG
CGATCTGGCTGAGCATTACGGCGTCGCCGTTTTGCCTGCTCGCTCGCGCAAGCCCAAGGACAAAGCCAAGGTCGAAGTCGGTGTTCAAGTGGTCGAGCGC
TGGATCCTCGCGGTGCTGCGCAACCGCCAGTTCTTCTCGCTCGGAGAACTCAACACAGCAATCAGCCTGCTACTCGAACGCCTCAATCACAAGCCCTTCA
AAAAGCTGCCCGGCTCGCGCCGGTCAGCCTTCGAAAGCATCGACCAACCTGCGCTGCAAGGGCTACCGGAGCATCCGTACGTCTACGCCGAATGGAAAAA
AGTGCGAGTCCATATCGACTATCACGTTGAGGTCGATGGCCACTTTTACTCGGTGCCGTACCAGTTGGTGAGGCACCAACTCGAAGTACGACTCACCGCC
CAGACCGTCGAGTGCTTCCACGCCAACCAGCGCGTGGCCAGCCACCTTCGCTCGCAGCACAAAGGCCGGCACACCACCCAAACCGAGCACATGCCCAAGA
GCCACCGCGAGCACGCCGAATGGACTCCACAGCGGTTGATCCGCTGGGCAGAACAGACCGGGCCTAACACCGCTGGCGTCATCGCCTACATCCTCGAGCG
CCGAATCCATCCGCAACACGGCTTCCGCGCCTGCCTGGGCATCCTGCGCCTGAGCAAACAGCACGGCGAAGCGCGGCTGGAAGCCGCGTGCCAGCGTGCG
CTGGCACTGGGCGCATGCAGTTACAAAAGCCTCGAATCGATCCTGCGCCAAGGGCTGGAAAAGCTACCGCTGTCCCAGCAAAACCTCCCGCTGCTGCCCG
ACGAGCACATCAACCTGCGCGGCCCTGGCTACTACCACTGACCTGAAAAAGGAGCTTCGAAATGCTACCGAATCCAACGCTCGACAAGCTGCAAACCCTG
CGCCTGCACGGCATGATCAAGGCGCTTGGCGAACAACACGCAACACCTGACATCAACGATCTCAGCTTCGACGAGCGCCTTGGCCTGATGGTCGACCGCG
AGCTGACCGAGCGCGAAGACGCCCGCATGACCACTCGCCTCAAAGCCGCGCGACTGCGCCACAACGCCTGCCTGGAAGACATCGACTACCGCAGCCCACG
CGGCCTGGACAAGGCCTTGATCCTGCAACTCGGCAGCGGCCAATGGCTGCGCGATGGCCTGAACCTGATCATCGGCGGGCCAACTGGTGTAGGCAAAACC
TGGCTCGCCTGCGCGCTGGCCCACAAGGCCTGCCGCGACGGCTACAGCGTCCGTTACCTGCGTCTACCGCGCTTGATGGAGGAGCTGGGCCTGGCCCACG
GCGACGGCCGCTTCGCCAAGCTGATGGCTGGCTACGCCAAGACTGACCTGCTGATCCTGGACGACTGGGGCCTGGCACCGTTTACCGCCGCCCAACGGCG
CGACATGCTTGAGCTGCTGGATGACCGCTACGGCAACCGCTCGACGCTGGTCACCAGCCAGATGCCGGTGGACAAATGGCATGCGCTGATCGGCGATCCG
ACCTTGGGCGACGCGATCCTCGACCGGCTGGTACATAACGCTTATCGGATCGAACTGAAGGGTGAATCGATGCGCAGGCGCGCAACAAAATTGACGTCGG
CAGGTGCTTCAGACTAACAATGCAAACCTGCGTCGCTGCGCTCCGACTGCCTGTCCAAGTGGACGTGGGGCAGGTGTCCAAGTGAGCTTGGAATGAGTGT
CCAAATGATCGTGGGCTGAGTGTCCAAGTGTCGTGGAATCCGCA
Protein section
ORF number : 2

 

ORF 1
LengthBeginEndStrandFusion ORF
1515 bp504 aa2271741+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MRKVREVLRLKFDAGLSVRKIAASLRISSGSAGNYLHRFNACGLSWPTSLSDAELERLLFPPAPTVPSDQRPMPDWAWVHAESRRPGVTLALLWQEYRLA
HPQGFQYSWFCEHYRLWAAKVDVVMRQEHRAGEKLFVDYAGQTAPIIDRSTGEIRQAQIFVAVLGASSYTFAEATWSQKLPDWLGSHVRCFAFFGGTTQI
LVPDNLRSGVTKAHRYEPDINPSYRDLAEHYGVAVLPARSRKPKDKAKVEVGVQVVERWILAVLRNRQFFSLGELNTAISLLLERLNHKPFKKLPGSRRS
AFESIDQPALQGLPEHPYVYAEWKKVRVHIDYHVEVDGHFYSVPYQLVRHQLEVRLTAQTVECFHANQRVASHLRSQHKGRHTTQTEHMPKSHREHAEWT
PQRLIRWAEQTGPNTAGVIAYILERRIHPQHGFRACLGILRLSKQHGEARLEAACQRALALGACSYKSLESILRQGLEKLPLSQQNLPLLPDEHINLRGP
GYYH

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
756 bp251 aa17622517+No
ORF function : Accessory Gene
AG : IS21 helper

ORF sequence :

MLPNPTLDKLQTLRLHGMIKALGEQHATPDINDLSFDERLGLMVDRELTEREDARMTTRLKAARLRHNACLEDIDYRSPRGLDKALILQLGSGQWLRDGL
NLIIGGPTGVGKTWLACALAHKACRDGYSVRYLRLPRLMEELGLAHGDGRFAKLMAGYAKTDLLILDDWGLAPFTAAQRRDMLELLDDRYGNRSTLVTSQ
MPVDKWHALIGDPTLGDAILDRLVHNAYRIELKGESMRRRATKLTSAGASD

 

Blast result :
Comments
ISPsy40 is 95% (ORFA : the transposase) and 99% (ORFB : the helper of transposition) aa similar to ISPsy14.
References
1] Butler M. (2017) Direct submission to ISfinder
2] Butler M.I., Stockwell P.A., Black M.A., Day R.C., Lamont I.L. and Poulter R.T. (2013) PLoS ONE 8 (2), E57464