ISfinder Mirror Site

ISBce21

Family IS1595
Group ISPna2

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
AHDJ01000072	ND	Bacillus cereus	Bacillus cereus BAG5X1-1

DNA section

IS Length : 1763 bp

Ends

IR Length : 25/29

IRL : GGCTGTTTTCGCATAGATTGTTGCTTTTTTGACCATACTATTTAGAGGTT
IRR : GGCTGTTTTCGTAAAGTTTGTTGCTCTTTAATATAATAAAATTTGATTAT

Insertion site

Left flank	Direct repeat	Right flank	DR Length
ACTTTATCTTT	T/ATAATTAA	AGACTTCTAT	8

DNA sequence

GGCTGTTTTCGCATAGATTGTTGCTTTTTTGACCATACTATTTAGAGGTTGATATAATGCGGTCTAAGGAGGCGTTCAAGTATGTGGAAAGATGTGTATG
AAGAGTTCAGTGAATTAACAAAATCAGAGAAGATGGCATTGTTTAATGCAATGAAGCAAGATTTGTTCCCAGATGAACCAGACAAAATAACAAAACTACT
AAAAAGTATTCGAGAAGCCCGCTTTGCATCTGGTATGGGGTGTGTTCATTGCGGAAGCACATCTGTAAAACGAAACGGAAAATATCGTTCAAGACAACGT
TATCTATGTAATGATTGCGGGAAATCGTTTAATGATATGACCAATACGCCATTTTCAGGCTCACGCTATCCTGAAAAATGGGTGAAATATATTGAACTTA
TGGTAGAGGGATATACCTTACCTAAAATCGCTGAACGTCTGAAAATTCATATCTCCACAGCATTTTATTGGAGACATAAAACCTTGAATGCATTAGGAAG
TCTTGGTTTCAATCAATTACAAGGTATCGTTGAAAGTGACGAAACTTTCTTTCGTGAGTCTATGAAAGGTCGTGAAATCACTCATAGAAAACCTAAGAAA
CGTGGCGAAAAAGACGAGAAGAGAGGGATTTCTAATCAAAAAATTGCTGTTGTAGTTGCACAAGATAGAAGCGGAAATGTGATTGCTCGCAAAGCGGGAA
CAGGTCGTGTAAGAGCCGAAGAAATAGATACTGTAATAGGTGAGTATATTCACCCTTTTTCTTTGCTATGTACAGATACAGCAACCAACTATAAAAAATT
TGCCAAAATTAAGGGATTACAGCACGAAACAGTTAATGAACGACTAAAACAACGTGTAAAGAAAGGTATTTACCATATACAACACGTCAACAATTTTCAT
AATCGCTTAAAAAAGTGGATGGAACGATTCCAAGGAGTTGCAACAAAGTATTTAGATAACTACCTCTATTGGTTTCGCTGGCTTGAAAATGGAAAACAAA
TAGCATTTGATGAACGTGTCAAACAAATGCTTGTTTCCGCCTGCCGAAAATCAAATCAATTTACAGTAGAGATGTTAAAAGCTACTTAAACGATTAATTT
TCAGGTATATATTTGTTACTTCCAGGGAGTTTCTTAATCTTGTTCCAAGCTTCAATCGCTTCTGCACGACTCTTCCCTTTATTTTTTGAGTCAGAGAAAA
AGTCACGAATGAATTGATTATATTCAAACTGAGGAGCAAGTTTCTTCTGGTAAGATGGGTCTTTCTTACGTTTTTCTTCTTCATTCCAAGCATTTACAAC
ATCATGATACGTTTTTCCAATATTGTTTTTAAAGTAGTTTTGGATATAGGTAGAAAAATGAAACTTTGGAATTACTGTCTTGAAAAAGGCTCTCACATCT
TGACTACAACGATGATTTTCCGTAATGACTGTATCAAGACTTAGTTCACTGTTAGTTGGAGGTTTATTATTAGCCTTTGAATTTCTTTGTGGTTTTTTTA
TCTCTCCTGTCCCAAGAAATGTTTCAATTCTATCAGAAATCTCTATCTTAGAGCCTGACGCACTTATTCCATTTTCTCTACAAAATGATTGCAATTCTTC
TTTTAACCAATAAAAATCTTTAAAATCCTGAATACTAATTTCTTTTGTAAGAATAGGTCTCATTCATAACACCTCCATATATACATATTCATATTATAGA
ACGTTTGTTCTTAATAATCAAATTTTATTATATTAAAGAGCAACAAACTTTACGAAAACAGCC

Protein section

ORF number : 2

ORF 1

Length		Begin	End	Strand	Fusion ORF
1008 bp	335 aa	82	1089	+	No

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MWKDVYEEFSELTKSEKMALFNAMKQDLFPDEPDKITKLLKSIREARFASGMGCVHCGSTSVKRNGKYRSRQRYLCNDCGKSFNDMTNTPFSGSRYPEKW
VKYIELMVEGYTLPKIAERLKIHISTAFYWRHKTLNALGSLGFNQLQGIVESDETFFRESMKGREITHRKPKKRGEKDEKRGISNQKIAVVVAQDRSGNV
IARKAGTGRVRAEEIDTVIGEYIHPFSLLCTDTATNYKKFAKIKGLQHETVNERLKQRVKKGIYHIQHVNNFHNRLKKWMERFQGVATKYLDNYLYWFRW
LENGKQIAFDERVKQMLVSACRKSNQFTVEMLKAT

Blast result :

ORF 2

Length		Begin	End	Strand	Fusion ORF
570 bp	189 aa	1663	1094	+	No

ORF function : Passenger Gene
Annotation : Hypothetical proteinDescription :

ORF sequence :

MRPILTKEISIQDFKDFYWLKEELQSFCRENGISASGSKIEISDRIETFLGTGEIKKPQRNSKANNKPPTNSELSLDTVITENHRCSQDVRAFFKTVIPK
FHFSTYIQNYFKNNIGKTYHDVVNAWNEEEKRKKDPSYQKKLAPQFEYNQFIRDFFSDSKNKGKSRAEAIEAWNKIKKLPGSNKYIPEN

Blast result :

Comments

ISBce21 is 80% (transposase) aa similar to ISBsp1.

References

1] ISfinder annotation (2016)
2] Feldgarden,M., Van der Auwera,G.A., Mahillon,J., Duprez,V., Timmery,S., Mattelet,C., Dierick,K., Sun,M., Yu,Z., Zhu,L., Hu,X., Shank,E.B., Swiecicka,I., Hansen,B.M., Andrup,L., Young,S.K., Zeng,Q., Gargeya,S., Fitzgerald,M., Haas,B., Abouelleil,A., Alvarado,L., Arachchi,H.M., Berlin,A., Chapman,S.B., Goldberg,J., Griggs,A., Gujja,S., Hansen,M., Howarth,C., Imamovic,A., Larimer,J., McCowen,C., Montmayeur,A., Murphy,C., Neiman,D., Pearson,M., Priest,M., Roberts,A., Saif,S., Shea,T., Sisk,P., Sykes,S., Wortman,J., Nusbaum,C. and Birren,B. (2012) Direct GenBank submission.