ISLca5
- Family IS1595
- Group ISPna2
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| AFYK01000056 | ND | Lactobacillus casei | Lactobacillus casei 21/1 Bacillus cereus MB.18 |
DNA section
IS Length : 1918 bp
Ends
IR Length : 23/29
IRL : GGCTATGTTAAAGAACTCTGTTGATATTTGATAAAAGAGAACGTTTGTTC
IRR : GGCTATGTTAAATTATATTGTTGATTTTTTACGAGAATTAAGGGAACTTC
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| AGATGGTGGTTTTT | TAATTAAA | AAACCCGCACAG | 8 |
DNA sequence
GGCTATGTTAAAGAACTCTGTTGATATTTGATAAAAGAGAACGTTTGTTCCATAATATAAGAAAAGAGTGAGAGCGAGTGGGTTCCGAATGTCATTGAAG
GAGATTATCGAAGAGATTCGAAAGTTAAATACAGCCGACCAACATAGATTGAAACAGTTCTTTGTGAACTCGCTTGCATCTTATTCGGCAAGCGAACCTG
TCTTCAAAGAAGTGTCTGAACGAAAGCATAAAGAAGGTTATACGTGTACCCATTGCCATTCGACGAAGGTTGTCCGTTTCGGGAAATACTCAGTGAAAAT
GGATATGCGACTGATTGAACGTCAACGTTATCGCTGTAAAGATTGCGGAAAGACCTTCACCGATGTTACGAATACCCCACTTCATCGAACTCACTTGCCT
CACAAATGGTTAGGGTTCATCCAATGTATGATAGAAGGCTACTCCCTTCGAAAAGCCTCTGAATTGATTGAGGTTCATTATGTCACTCTCTTCTATTGGC
GACATAAACTGCTTTCTGCGATGAAGCAGATGGACTTTGAGAAGTTCGAAGGTATTGTCGAGATGGATGAAACCTATTTCCTGTATTCTGAGAAAGGAAA
GCGTAATCTCAAAGGTCGTAAACCAAGAAAACGTGGCGGCTCCTCGAAGAAACGGGGCATTAGTAAAGACCAAGTGTGCGTTCTTGTCGCAAGAGACCGC
CAAAAGATGACGTATTCAAAAATCATCGGTCAAGGACGTATCGTAAAGACTCGACTTGATGATGCCATCGGCTCCAAACTTTCTTCAACGAATGTTCTCT
GTACAGACGCTTGGAGAGCCTTTAAGACCTATGCGACCGATAAAGGTTTGGAACATTACCGATTCAAATCAGATGGATATCAACGTGTGAAAGGTGTTTA
TCACATCCAGAACGTTAATAGTTATCATAGCCGACTCAAAGGCTGGATTGACCGTTTCAATGGCGTCGCTACAAAGTACCTCGACCATTATTTGAGTTGG
TTCCGATTCCTCGATAGCATCAGACATCGCAACAACGATGCAACCGTCAGCAAAATGATAATAGAAAGTTGTTTATTCTCAACAGTAGAAACCTATGATA
AACTTAGATTATCAGAATTTTCCAAATGATAATCTAAGTTGTTCAAGAATAGGGGCTGTATGATTTGGTCTATAAATTATGACCATTTAGGAAGTTTTAA
TCATATCAAAAGTAGAGAAAAAATATTCAAAGCTATATTTTCTTAAAATTTCCATTAGTCAAAGAGAGGAGATTAACTATGATAGACAAAAATGAGAAAG
AGATTTTATTATTGAATAGCGAGAAAAATTTCAAAGAACTACTTGAAATAATTGAATCAGTACCGAAAAGTAAAAGAGGAATTTCTATTGAAACTCCAGA
GAGAGATAAAAATTTTAGAGATGTTTTAATGCATCTTTATGAATGGCACGCAATGCTTGAGAGATGGTATAGAGAGGGTATGGATGGGGATATTCCTTTT
ATGCCAGCACCTGGTTATAAGTGGAGTAGTATTAAACTCCTGAATATACGGATTTGGGAGAACTATCAAGATGTGACATTAAATCAAGCTATAAAAAAAT
TGAAGTTAAGTCACCAAAGAGTAACAGACTTAATAAAAATGCATACGAATGAAGAAATCGTGACAAAAAATATTATAAGTGGACGAAAACAAGTAATTTG
TTCAGTTATTTTACAGCGAACACATCTAATCATTACATTTGGGCTGTGAAAAAATGTGAAGTTATCGCAAAGGCTTTAAAAGAAATAGAAAATGAAAATT
TTGTAAAAACGTCTACTTAAACGAACGAGGACTTTAATAGAGTAAACAAAAGGGAACTTCTAAAAATGGAAGTTCCCTTAATTCTCGTAAAAAATCAACA
ATATAATTTAACATAGCC
GAGATTATCGAAGAGATTCGAAAGTTAAATACAGCCGACCAACATAGATTGAAACAGTTCTTTGTGAACTCGCTTGCATCTTATTCGGCAAGCGAACCTG
TCTTCAAAGAAGTGTCTGAACGAAAGCATAAAGAAGGTTATACGTGTACCCATTGCCATTCGACGAAGGTTGTCCGTTTCGGGAAATACTCAGTGAAAAT
GGATATGCGACTGATTGAACGTCAACGTTATCGCTGTAAAGATTGCGGAAAGACCTTCACCGATGTTACGAATACCCCACTTCATCGAACTCACTTGCCT
CACAAATGGTTAGGGTTCATCCAATGTATGATAGAAGGCTACTCCCTTCGAAAAGCCTCTGAATTGATTGAGGTTCATTATGTCACTCTCTTCTATTGGC
GACATAAACTGCTTTCTGCGATGAAGCAGATGGACTTTGAGAAGTTCGAAGGTATTGTCGAGATGGATGAAACCTATTTCCTGTATTCTGAGAAAGGAAA
GCGTAATCTCAAAGGTCGTAAACCAAGAAAACGTGGCGGCTCCTCGAAGAAACGGGGCATTAGTAAAGACCAAGTGTGCGTTCTTGTCGCAAGAGACCGC
CAAAAGATGACGTATTCAAAAATCATCGGTCAAGGACGTATCGTAAAGACTCGACTTGATGATGCCATCGGCTCCAAACTTTCTTCAACGAATGTTCTCT
GTACAGACGCTTGGAGAGCCTTTAAGACCTATGCGACCGATAAAGGTTTGGAACATTACCGATTCAAATCAGATGGATATCAACGTGTGAAAGGTGTTTA
TCACATCCAGAACGTTAATAGTTATCATAGCCGACTCAAAGGCTGGATTGACCGTTTCAATGGCGTCGCTACAAAGTACCTCGACCATTATTTGAGTTGG
TTCCGATTCCTCGATAGCATCAGACATCGCAACAACGATGCAACCGTCAGCAAAATGATAATAGAAAGTTGTTTATTCTCAACAGTAGAAACCTATGATA
AACTTAGATTATCAGAATTTTCCAAATGATAATCTAAGTTGTTCAAGAATAGGGGCTGTATGATTTGGTCTATAAATTATGACCATTTAGGAAGTTTTAA
TCATATCAAAAGTAGAGAAAAAATATTCAAAGCTATATTTTCTTAAAATTTCCATTAGTCAAAGAGAGGAGATTAACTATGATAGACAAAAATGAGAAAG
AGATTTTATTATTGAATAGCGAGAAAAATTTCAAAGAACTACTTGAAATAATTGAATCAGTACCGAAAAGTAAAAGAGGAATTTCTATTGAAACTCCAGA
GAGAGATAAAAATTTTAGAGATGTTTTAATGCATCTTTATGAATGGCACGCAATGCTTGAGAGATGGTATAGAGAGGGTATGGATGGGGATATTCCTTTT
ATGCCAGCACCTGGTTATAAGTGGAGTAGTATTAAACTCCTGAATATACGGATTTGGGAGAACTATCAAGATGTGACATTAAATCAAGCTATAAAAAAAT
TGAAGTTAAGTCACCAAAGAGTAACAGACTTAATAAAAATGCATACGAATGAAGAAATCGTGACAAAAAATATTATAAGTGGACGAAAACAAGTAATTTG
TTCAGTTATTTTACAGCGAACACATCTAATCATTACATTTGGGCTGTGAAAAAATGTGAAGTTATCGCAAAGGCTTTAAAAGAAATAGAAAATGAAAATT
TTGTAAAAACGTCTACTTAAACGAACGAGGACTTTAATAGAGTAAACAAAAGGGAACTTCTAAAAATGGAAGTTCCCTTAATTCTCGTAAAAAATCAACA
ATATAATTTAACATAGCC
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1041 bp | 346 aa | 89 | 1129 | + | No |
Chemistry : DDE
ORF sequence :
MSLKEIIEEIRKLNTADQHRLKQFFVNSLASYSASEPVFKEVSERKHKEGYTCTHCHSTKVVRFGKYSVKMDMRLIERQRYRCKDCGKTFTDVTNTPLHR
THLPHKWLGFIQCMIEGYSLRKASELIEVHYVTLFYWRHKLLSAMKQMDFEKFEGIVEMDETYFLYSEKGKRNLKGRKPRKRGGSSKKRGISKDQVCVLV
ARDRQKMTYSKIIGQGRIVKTRLDDAIGSKLSSTNVLCTDAWRAFKTYATDKGLEHYRFKSDGYQRVKGVYHIQNVNSYHSRLKGWIDRFNGVATKYLDH
YLSWFRFLDSIRHRNNDATVSKMIIESCLFSTVETYDKLRLSEFSK
THLPHKWLGFIQCMIEGYSLRKASELIEVHYVTLFYWRHKLLSAMKQMDFEKFEGIVEMDETYFLYSEKGKRNLKGRKPRKRGGSSKKRGISKDQVCVLV
ARDRQKMTYSKIIGQGRIVKTRLDDAIGSKLSSTNVLCTDAWRAFKTYATDKGLEHYRFKSDGYQRVKGVYHIQNVNSYHSRLKGWIDRFNGVATKYLDH
YLSWFRFLDSIRHRNNDATVSKMIIESCLFSTVETYDKLRLSEFSK
Blast result :
Comments
ISLca5 is 89% aa similar to ISBce20.
References
1] ISfinder annotation (2016)
2] Broadbent,J.R., Neeno-Eckwall,E.C., Stahl,B., Tandee,K., Cai,H., Morovic,W., Horvath,P., Heidenreich,J., Perna,N.T., Barrangou,R. and Steele,J.L. (2012) BMC Genomics 13 (1), 533.
3] Crovadore,J., Calmin,G., Tonacini,J., Chablais,R., Schnyder,B., Messelhausser,U. and Lefort,F. (2016) Genome Announc 4 (3).
2] Broadbent,J.R., Neeno-Eckwall,E.C., Stahl,B., Tandee,K., Cai,H., Morovic,W., Horvath,P., Heidenreich,J., Perna,N.T., Barrangou,R. and Steele,J.L. (2012) BMC Genomics 13 (1), 533.
3] Crovadore,J., Calmin,G., Tonacini,J., Chablais,R., Schnyder,B., Messelhausser,U. and Lefort,F. (2016) Genome Announc 4 (3).