ISfinder Mirror Site

ISGli1

Family IS1595
Group ISPna2

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
JH594606	ND	Gillisia limnaea	Gillisia limnaea DSM 15749

DNA section

IS Length : 1072 bp

Ends

IR Length : 15/19

IRL : GGCTCTAGTTTAAACCAGCTATTATTTGTATATTGAGTTTATGGAAACAG
IRR : GGCTCTAGTTTAGCACTGCGTTGATATTAATTTTTCATAGTTTGGCTGGA

Insertion site

Left flank	Direct repeat	Right flank	DR Length
TGAATAGTTA	ATAAGTAT	TTAAATTCAG	8

DNA sequence

GGCTCTAGTTTAAACCAGCTATTATTTGTATATTGAGTTTATGGAAACAGAAAAGCTTAAAGAACAAATACTCTCACTGCCAGTTGCGCAGCGTGAGCGT
TTATTGAAGGAAATCGAAGATTCGACCAAAAACATAGACCTGTCCGCGATTGTCTCACGCCGGCACGAGTTTGACAACAATCTCGGTGCCTGTCCGCACT
GCTCTCACCCTAAGTATGTCCGCTTTGGTATGGACAAAGGTGCGCAGCGCTACAAGTGCAAGTCTTGCCACAGGAGTTTTACTGAGTACACTGGCACTTG
GATGGCAGGCCTTCAAAGGAAGGACAAGATTGCAGATTACCTAAAGCTAATGGTCGAAGAAAAGAGCCTTGACAAAATTGTCTCGGCATTGGGCATCAAC
AAAAAGACAGCCTTTGACTGGCGTCACAAGATATTGGCCTCACTGGGCGCGGACGACGGGGATGACTTCACCGGTATCACTGAAAGTGATGAGGCTTTCT
TCCTCAGGTCTGAAAAGGGTATGGTGGTCAAAAACCGAAAACCAAGGAAAAGGGGTGGCAAGTCCAGCAAGAGGGGCATAAGCAATGATCAGGTTGCGGT
CATCGTGACCCAGGACAGAAATTCTGGAATAGATTTGAGCGTGGCCACGCTTGGACGGATTGGGAAGGCCGATATTGCAAATGCCATTGGCAAACGCATA
AAAGAGGGAAGCACAATATTGTGTAGTGATTCCCGCCACAGCTATAAAGGCTTCGCCAAGGACACCAAGGTGGAGTTCCATCCCATAAATGTTTCCAAAG
ATGAAAGGGTAAAAGGTGCATTCCATGTGCAACATGTAAACTCGACACACAACAGGATAAAAAAGTGGATTGGCAGTACGTTCTGGGGCGTGTCCACCAA
ATACCTGCAACAGTATCTAAACTGGCATGCCCTAAAAGAAAAGATAAAATTAAATAGGGACACCACTGCTGCCTTCGCACAACAAACACTGGGTGTGGGG
GCTTTGAAGCGTTTTGCGAACATCCAGCCAAACTATGAAAAATTAATATCAACGCAGTGCTAAACTAGAGCC

Protein section

ORF number : 1

ORF 1

Length		Begin	End	Strand	Fusion ORF
1023 bp	345 aa	41	1063	+	No

ORF function : Transposase
Chemistry : DDE

ORF sequence :

METEKLKEQILSLPVAQRERLLKEIEDSTKNIDLSAIVSRRHEFDNNLGACPHCSHPKYVRFGMDKGAQRYKCKSCHRSFTEYTGTWMAGLQRKDKIADY
LKLMVEEKSLDKIVSALGINKKTAFDWRHKILASLGADDGDDFTGITESDEAFFLRSEKGMVVKNRKPRKRGGKSSKRGISNDQVAVIVTQDRNSGIDLS
VATLGRIGKADIANAIGKRIKEGSTILCSDSRHSYKGFAKDTKVEFHPINVSKDERVKGAFHVQHVNSTHNRIKKWIGSTFWGVSTKYLQQYLNWHALKE
KIKLNRDTTAAFAQQTLGVGALKRFANIQPNYEKLISTQC

Blast result :

Comments

ISGli1 is 80% aa similar to ISPto1.

References

1] ISfinder annotation (2016)
2] Lucas,S., Copeland,A., Lapidus,A., Bruce,D., Goodwin,L., Pitluck,S., Peters,L., Mikhailova,N., Held,B., Kyrpides,N., Mavromatis,K., Detter,J.C., Han,C., Land,M., Hauser,L., Markowitz,V., Cheng,J.-F., Hugenholtz,P., Woyke,T., Wu,D., Tindall,B., Pomrenke,H., Brambilla,E., Klenk,H.-P. and Eisen,J.A. (2011) Direct GenBank submission.