ISfinder Mirror Site

ISPels1

Family IS1595
Group ISPna2

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
LMDC01000023	ND	Pelomonas sp.	Pelomonas sp. Root1217

DNA section

IS Length : 1166 bp

Ends

IR Length : 21/24

IRL : GGCTCTTTGCGCATTGATTCGTGATGGCTCACGTGTTGAGCCTGTGGGNA
IRR : GGCTCTTTGCGCATTGCTTGATGACGCATCAGGCACCGACAGCCAGAGCC

Insertion site

Left flank	Direct repeat	Right flank	DR Length
CCTTTGGGCCGCT	TTTGTTTT	TGCGATGCACAATCC	8

DNA sequence

GGCTCTTTGCGCATTGATTCGTGATGGCTCACGTGTTGAGCCTGTGGGNAGGGATATTGGCTCGCACGGCCCCGGAGTCTGGGGCGACGGGAGTCACAGA
TGCGCAGCCGAGAACTCAAGAGGATGCTGGCCGAGGTATCGAAGCTGACGCCGGGGCAGCGGCAGGAGTTGATGCAGGCNTTGAGCGGCGCCGACGAGGT
CGTGCAGGTCGTACAGACGGTGCAATCGCGACCGCTGGCCTGTCCGCACTGCCAAGGTGAGCGCGTGGTTCGCAATGGACACGCCAGCGGGTTGCAACGC
TACAAATGCCGCAGNTGCACCAGGACCTTNAACTCGCTGACCGANACGCCGCTGGCTCGGCTGCGGCAAAAGGGGAAATGGGAGCGCCAAGCCCAAGTGC
TGCGCCAGGGCTTGAGCGTGCATCAGGCCGCGGACANCCTGTCGGTGGCTCCCAGCACGGCCTTCCGCTGGCGTCACCGCTTCCTGCAACTGGCCCAGGG
CGTGAAGGCCCGAGTGCTGCAAGGCGTGGTGGAGGCCGACGAGACCTACTTCCTGCGCTCCAGCAAGGGCCAACACGTGCAGGCCCGCAAGGCTCGACGC
CGAGGCGGCAGCGGAGCCAAGCGCAGGCTGTCCGACGAACAAGAGCCGGTGTTGGTGGCGCGAGACCGCTCCGGCGCAACGGCCGATTTCATCCTGCAAC
GCGCCGGCAAGGCTCAGACCATCGCGGCGTTGGCGCCGGTACTGGCCCCGGACGCTGTGCTGTGCACGGACTGCAGTGGCTCGTTAGGAGCAGCAGCCAA
GCATTTGGGCGTTGAGCACCATGCGCTGAATCTCTCCCGAGGTGAGCGGGTGCAGGGCGCTTGGCACATCCAGAACGCCAACGCGTATCACGCCAGGCTC
AAGAACTGGATACGCCGCTTCAATGGGGTGGCCAGTTCGTACCTGGCCAGCTACCTGGGCTGGTTCCGCGCCTTGGACCGGTCAGCCCAAACTCACCGAC
AGCCCGCGCCGTTCCTGGCTCTGGCTGTCGGTGCCTGATGCGTCATCAAGCAATGCGCAAAGAGCCTTTNCCTTGGACCGGTCAGCCCAAACTCACCGAC
AGCCCGCGCCGTTCCTGGCTCTGGCTGTCGGTGCCTGATGCGTCATCAAGCAATGCGCAAAGAGCC

Protein section

ORF number : 1

ORF 1

Length		Begin	End	Strand	Fusion ORF
939 bp	312 aa	100	1038	+	No

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MRSRELKRMLAEVSKLTPGQRQELMQALSGADEVVQVVQTVQSRPLACPHCQGERVVRNGHASGLQRYKCRSCTRTFNSLTETPLARLRQKGKWERQAQV
LRQGLSVHQAADLSVAPSTAFRWRHRFLQLAQGVKARVLQGVVEADETYFLRSSKGQHVQARKARRRGGSGAKRRLSDEQEPVLVARDRSGATADFILQR
AGKAQTIAALAPVLAPDAVLCTDCSGSLGAAAKHLGVEHHALNLSRGERVQGAWHIQNANAYHARLKNWIRRFNGVASSYLASYLGWFRALDRSAQTHRQ
PAPFLALAVGA

Blast result :

Comments

ISPels1 is 71% aa similar to ISBeb2.

References

1] ISfinder annotation (2016)
2] Bai,Y., Muller,D.B., Srinivas,G., Garrido-Oter,R., Potthoff,E., Rott,M., Dombrowski,N., Munch,P.C., Spaepen,S., Remus-Emsermann,M., Huttel,B., McHardy,A.C., Vorholt,J.A. and Schulze-Lefert,P. (2015) Nature 528 (7582), 364-369.