ISCfr14

  • Family IS66
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
NC_019360 ND Citrobacter freundii
Citrobacter freundii
Citrobacter freundii plasmid pNDM-CIT
DNA section
IS Length : 2442 bp

Ends


IR Length : 21/22

IRL : GTAAGCGTACAGCGTGAGCCGTCTGGTCATAATCCGAAGCATCCGACAAA
IRR : GTAAGCGTACAGCGAGAGCCGTATTGACGGGTACGGGTTATTCAGTTTGC

Insertion site


Left flankDirect repeatRight flankDR Length
GGCCCTGGTAATAAAGTCTGGCCCAGTA8

DNA sequence

GTAAGCGTACAGCGTGAGCCGTCTGGTCATAATCCGAAGCATCCGACAAAGTGGTGTCCACCAAATAAGTAGTGGGAACCAAAGTGTCAGATATGCAGAA
AAATGTGACTCCCGGCAGACGTAAAGGCTGTCCTAATTATTCTCCAGAATTTAAGCAGCAACTTGTTGCTGCCTCCTGCGAACCAGGAATATCCATTTCA
AAACTTGCGCTTGAAAATGGCATTAACGCCAATCTGCTGTTCAAATGGCGCCAACAATGGCGCGAGGGAAAGCTGCTATTACCTTCTTCAGAGAGTCCTC
AGCTACTTCCTGTGACTCTCGATGCCGCTGACGTACAGCCAGAACCGCCCACTGAGAGCCCGGAAGCCCTCAGTATCAGCTGTGAGATAACGTTCCGGCA
CGGGACGCTCCGCCTCAACGGCACTGTCAGCGAAAAGTTCCTGGCTCTGCTGATACAGGAGCTGAAGCGATGATCCCGTTACCAACAGGTACCAAAATCT
GGCTGGTTGCCGGTACCACTGATATGAGAAATGGCTTCAACGGTCTTGCCGCAAAAGTACAGACGGCGCTGAAAGACGATCCGATGTCCGGCCACGTCTT
CATCTTCCGGGGTCGCAGCGGCAACCAGGTCAAACTGCTGTGGTCCACCGGCGACGGGCTGTGCCTGCTGACCAAACGACTGGAGCGCGGTCGCTTCGCC
TGGCCCTCTGCCCGTGATGGCAAAGTATTTCTGACTCCGGCACAGCTGGCGATGCTGATGGAAGGCATCGACTGGCGACAACCTAAACGGTTGCTGACAT
CTCTGACCATGTTGTAGGCCTCTTTATCCTGGTTGTTGCTGAATAAGCCTGGTAAAATACGGGCTTATGAACAACACCTCTTCTGACGAAATCCTTCGGC
TGAAACAGCGGCTTGCCGAACAGGAGGCGCTGAACCGCGCCCTGCTGGAAAAGCTGGCCGACCGTGAGCGCGAAATAGACCACCTGCAGGCGCAGCTGGA
TAAACTCCGCCGGATGAACTTCGGCAGCCGTTCCGAAAAAGTATCCCGTCGTATCGCACAGATGGAAGCTGACCTGAAGGTGCTTCAGAAAGAAAGCGAT
ACACTGACCGGTCGTGTGGATGACCCGGAAGTGCAGCGCTCGCTGCGTCAGACCCGCACCCGCAGACCGTTCCCCGAATCACTCCCGCGTGATGAAAAGC
GTATACTTCCAGCCGAATCCTGTTGCCCGGAGTGCGGTGGTTCGCTGAGTTACCTGGGTGAAGATGCCGCCGAACAGCTGGAGCTGATGCGCAGCGCCTT
CCGCGTTATCCGGACAGTACGTGAAAAACATGCCTGTACTCAGTGTGATGCGATAGTGCAGGCAGCCGCGCCTTCACGGCCCATCGAGCGGGGTATCGCC
GGCCCGGGGTTGCTGGCCCGCGTGCTGACCTCAAAGTATGCCGAGCACACACCGCTGTACCGCCAGTCTGAAATATACGGCCGACAGGGCGTGGAGCTGA
GTCGCTCACTGCTGTCGGGCTGGGTGGATGCATGTTGCCGGCTGCTGTCACCGCTGGAAGAGGCGCTTCAGGACTATGTCCTGACCGACGGCAAACTCCA
CGCCGATGACACGCCCGTCCCGGTACTGTTACCAGGTAATAAAAAGACGAAGACCGGACGGTTGTGGACGTACGTTCGTGATGACCGCAACGCCGGGTCA
GCGCTGGCGCCGGCTGTATGGTTCGCTTACAGTCCGGACAGAAAAGGCATCCACCCGCAGAGCCATCTTGCCGGCTTCAGCGGTGTGCTGCAGGCGGATG
CGTATGCCGGGTTCAACGAACTGTACCGCAATGGACAGATAACGGAAGCGGCCTGCTGGGCTCACGCCCGCCGCAAGATCCACGATGTGCACGTTCGCAC
GCCATCAGCGCAGACAGAGGAAGCCCTGAAACGTATCGGCGACCTGTATGCCATTGAGGCTGAAATACGGGGAATGCCGGCAGAGCAACGCCTTGCTGAA
CGTCAGCAAAAAGCGAAACCACGACTGAAATCTCTGGAAAGCTGGCTGCGTGAAAAGATAAAAACCCTGTCGCGACACTCAGAGCTGGCGAAGGCGTTCG
CGTACGCACTGAACCAGTGGCCGGCACTGACGTACTATGCAGACGATGGCTGGGCTGAAGCCGATAACAATATCGCTGAAAATGCGCTGCGGACGGTCAG
CCTGGGCCGCAAAAACTTCCTGTTCTTCGGCTCTGACCACGGTGGTGAGCGCGGAGCACTGCTGTACAGCCTGATCGGTACATGCAAACTGAACGGCGTG
GAGCCAGAGAGCTATCTCCGTCATGTGCTCAACGTCATAGCTGACTGGCCGGTCAATCAGGTCAGCGAACTGTTACCATGGCGCGTAACCCTGCAAACTG
AATAACCCGTACCCGTCAATACGGCTCTCGCTGTACGCTTAC
Protein section
ORF number : 3

 

ORF 1
LengthBeginEndStrandFusion ORF
402 bp133 aa72473+No
ORF function : Accessory Gene
AG : IS66 TnpA

ORF sequence :

VGTKVSDMQKNVTPGRRKGCPNYSPEFKQQLVAASCEPGISISKLALENGINANLLFKWRQQWREGKLLLPSSESPQLLPVTLDAADVQPEPPTESPEAL
SISCEITFRHGTLRLNGTVSEKFLALLIQELKR

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
348 bp115 aa470817+No
ORF function : Accessory Gene
AG : IS66 TnpB

ORF sequence :

MIPLPTGTKIWLVAGTTDMRNGFNGLAAKVQTALKDDPMSGHVFIFRGRSGNQVKLLWSTGDGLCLLTKRLERGRFAWPSARDGKVFLTPAQLAMLMEGI
DWRQPKRLLTSLTML

 

Blast result :
ORF 3
LengthBeginEndStrandFusion ORF
1539 bp512 aa8672405+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MNNTSSDEILRLKQRLAEQEALNRALLEKLADREREIDHLQAQLDKLRRMNFGSRSEKVSRRIAQMEADLKVLQKESDTLTGRVDDPEVQRSLRQTRTRR
PFPESLPRDEKRILPAESCCPECGGSLSYLGEDAAEQLELMRSAFRVIRTVREKHACTQCDAIVQAAAPSRPIERGIAGPGLLARVLTSKYAEHTPLYRQ
SEIYGRQGVELSRSLLSGWVDACCRLLSPLEEALQDYVLTDGKLHADDTPVPVLLPGNKKTKTGRLWTYVRDDRNAGSALAPAVWFAYSPDRKGIHPQSH
LAGFSGVLQADAYAGFNELYRNGQITEAACWAHARRKIHDVHVRTPSAQTEEALKRIGDLYAIEAEIRGMPAEQRLAERQQKAKPRLKSLESWLREKIKT
LSRHSELAKAFAYALNQWPALTYYADDGWAEADNNIAENALRTVSLGRKNFLFFGSDHGGERGALLYSLIGTCKLNGVEPESYLRHVLNVIADWPVNQVS
ELLPWRVTLQTE

 

Blast result :
Comments
ISCfr14 is 91% (orfA), 98% (orfB) and 93% (orfC) aa similar to ISEc8.
References
1] Zhou D. (2016) Direct submission to ISfinder
2] Dolejska,M., Villa,L., Poirel,L., Nordmann,P. and Carattoli,A. (2013) J. Antimicrob. Chemother. 68 (1), 34-39