ISfinder Mirror Site

ISHti15

Family IS1595
Group ISH4

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
HF571521	ND	Halorhabdus tiamatea	Halorhabdus tiamatea SARL4B plasmid pHTIA

DNA section

IS Length : 996 bp

Ends

IR Length : 22/25

IRL : CGCTCTTGCGAAGTAGATTGTTGATAGCGAGAGCTGCGGCTGAAAGTCGA
IRR : CGCTCTTGTGAAGCACATTGTTGATTTTCAGAGGGCGGCGTCGAGAGCAT

Insertion site

Left flank	Direct repeat	Right flank	DR Length
ACAAAATAGTGAGAA		TTCTTGGAAATCCG	0

DNA sequence

CGCTCTTGCGAAGTAGATTGTTGATAGCGAGAGCTGCGGCTGAAAGTCGAAGAGGGCAAGGGCACCGCGCTAGCGGTGCCCGATATGTTCCCGATAAACC
GATTCGTGTCGGAATCGGCTGCAGCGGACCTGCTCCAACAGGTTCGCTGGCGTGATGGCGTCGAGTGCCCCCGCTGCCGTTCTGACCTGACGGTCAGAAA
CGGCAGCTATCGGGCGTATCAACGGTATCTGTGTAAGAATTGCGGTCGGACGTTCAACGACAAGACCGGCACAATTTTCGCCCACTCGAAGCTTTCACTG
AAAGAGTGGTACTTCACGATCTACGTGTTCTTACGGTTTAACACGAGTATTCGGCAAATCGAGGCGGAACTCGATCTGTCGTACCGAACGGTGAGACGGC
GCGTCGAGCGCTTCGCCAAGGCGCTCGACGCGCCTTCGATCACGCTGTCCGGGCCGGTCGAAATCGACGAAGTGTACGTCTCGGCCGGCCTCAAAGGCCG
CGAGCGCGACCAGGCGTCGCGCTCGCGTGGCCTATCCACGCGTGGACGAGGATCGTACGACGGCGACAAACCGCCGGTGTTCACGCTGGTCGATCGTGGC
TCGGACGAGCGCTACGTCGTGCCAGCGAAATCCGCCGAGGAATCGACCGTGCGACTCCTGCTCGGCAACCGCACACAGGAGTCGCTGACCGTCTATACGG
ATGGATTTCGGGCGTACGAACCGCTCGAAGAAGACAGCGCATTCACCCGCAAATACGTCGTCCACGGCGACGGCGAATACGCTGACGGCGACATCCACGT
CAACACCTGCGAGAGCCACGCGTCGCTGACGCGACGGTGGCTCTCGCCCCATCGAGGAGTGTCAAAAGACAAGCTAACACCGTATCTGAGAGCCTTTCAG
CTCCGCCGCGAACTCTACAGAAAACCTGGTGACGAAGCACTCAAACATGCTCTCGACGCCGCCCTCTGAAAATCAACAATGTGCTTCACAAGAGCG

Protein section

ORF number : 1

ORF 1

Length		Begin	End	Strand	Fusion ORF
885 bp	294 aa	85	969	+	No

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MFPINRFVSESAAADLLQQVRWRDGVECPRCRSDLTVRNGSYRAYQRYLCKNCGRTFNDKTGTIFAHSKLSLKEWYFTIYVFLRFNTSIRQIEAELDLSY
RTVRRRVERFAKALDAPSITLSGPVEIDEVYVSAGLKGRERDQASRSRGLSTRGRGSYDGDKPPVFTLVDRGSDERYVVPAKSAEESTVRLLLGNRTQES
LTVYTDGFRAYEPLEEDSAFTRKYVVHGDGEYADGDIHVNTCESHASLTRRWLSPHRGVSKDKLTPYLRAFQLRRELYRKPGDEALKHALDAAL

Blast result :

Comments

ISHti15 is 90% aa similar to ISHvo5.

References

1] ISfinder annotation (2016).
2] Werner,J., Ferrer,M., Michel,G., Mann,A.J., Huang,S., Juarez,S., Ciordia,S., Albar,J.P., Alcaide,M., La Cono,V., Yakimov,M.M., Antunes,A., Taborda,M., da Costa,M.S., Hai,T., Glockner,F.O., Golyshina,O.V., Golyshin,P.N. and Teeling,H. (2014) Environ. Microbiol. Aug;16(8):2525-37.