ISfinder Mirror Site

ISPlba2

Family IS1595
Group ISSod11

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
MHYY01000129	ND	Planctomycetes bacterium	Planctomycetes bacterium RIFCSPLOWO2_12_38_17

DNA section

IS Length : 1024 bp

Ends

IR Length : 23/24

IRL : TGTGGTTATTCAGTTGACGCAAGTAACCCCAACATATAGTATGTTGGGGT
IRR : TGTGGTTATTCAGTTGACTCAAGTCCATACAATATGTTGTGGTGCTTCAA

Insertion site

Left flank	Direct repeat	Right flank	DR Length
CATTGAATCAAA	TTTTTTTA	TTTCAAGCTACA	8

DNA sequence

TGTGGTTATTCAGTTGACGCAAGTAACCCCAACATATAGTATGTTGGGGTATGGAAGACTATCCTCGGACACTTTTTGAGTTTGAAGCACGATTTAGCAC
TGAAGCGTCGTGCAGGGAATATCTTTTTAGATTACGCTGGCCAGACGGGTTTGTTTGCCCTCGATGTCAGAGTAGAAAAGCATGGGTAACGCAACGTGAT
TTATTGGTCTGTGCATCCTGCGGAGACCATGTTTCTGTAACGGCAGGAACAATCTTTCAAGATACTCGCAAGCCACTAACAATGTGGTTTAGAGCAATGT
GGTGGGTAACAATCCAGAAAACCGGAGTTAGTGCGATAGGCTTGCAAGGTATTCTTGGGTTACGCAGCTATTATACTGCGTGGACATGGCTTCAAAAACT
TAGAAGAGCGATGGTTAGACCAGGCAGAGAGTGTCTTTCTGGTATGGTCGAAGTGGATGAAACCTATATTGGAGGAGAAAAGCTGGGCAAACGAGGTCGA
GGAGCCTTAGGTAAGAGCTTAGTAATAATAGCGGCAGAGGTAAAGGGAAATCGCATTGGTCGGATTAGGCTACAATGCATAAGAAACGCCTCTGCTAAAA
GCGTGGAAGAAGCTGTCCAGCAAATTGTAGAACAAGGGAGTATTGTTCAAACGGACGATTGGAAAGGCTATCATGGGCTGGATCATCTTGGTTATGCACA
TGAAGTTATGCGAAGAAATACAGAAATTGGTGAGAACTTATTGCCACATTGCCACAGGGTGGCTGCCTTGCTTAAACGATGGATAATGGGGACTCATCAA
GGTTCGATCAGCCATGAACATTTGGAGCACTATCTTGATGAGTTCGTATTCAGGTTTAATAGAAGAAACTGCAGACACAGAGGGAAACTTTTCTATCGTC
TTGCACAACAGGCCGTCACGATTGGGTCAACATCTTACAAAGATATGGTCAAATGCGGTAGAAGAGGGCGAAAATTGAAGCACCACAACATATTGTATGG
ACTTGAGTCAACTGAATAACCACA

Protein section

ORF number : 1

ORF 1

Length		Begin	End	Strand	Fusion ORF
969 bp	322 aa	51	1019	+	No

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MEDYPRTLFEFEARFSTEASCREYLFRLRWPDGFVCPRCQSRKAWVTQRDLLVCASCGDHVSVTAGTIFQDTRKPLTMWFRAMWWVTIQKTGVSAIGLQG
ILGLRSYYTAWTWLQKLRRAMVRPGRECLSGMVEVDETYIGGEKLGKRGRGALGKSLVIIAAEVKGNRIGRIRLQCIRNASAKSVEEAVQQIVEQGSIVQ
TDDWKGYHGLDHLGYAHEVMRRNTEIGENLLPHCHRVAALLKRWIMGTHQGSISHEHLEHYLDEFVFRFNRRNCRHRGKLFYRLAQQAVTIGSTSYKDMV
KCGRRGRKLKHHNILYGLESTE

Blast result :

Comments

ISPlba2 is 70% aa similar to ISUnb3.

References

1] ISfinder annotation (2016)
2] Anantharaman,K., Brown,C.T., Hug,L.A., Sharon,I., Castelle,C.J., Probst,A.J., Thomas,B.C., Singh,A., Wilkins,M.J., Karaoz,U., Brodie,E.L., Williams,K.H., Hubbard,S.S. and Banfield,J.F. (2016)Nat Commun 7, 13219.