ISfinder Mirror Site

ISUnb3

Family IS1595
Group ISSod11

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
METG01000176	ND	unclassified Bacteria	unclassified Bacteria Candidate division NC10 bacterium RBG_16_65_8

DNA section

IS Length : 1030 bp

Ends

IR Length : 24

IRL : TAGGGGTATCTCCTTGACTCCAGTACCCCCAAGATATAGCGTACCGGGCC
IRR : TAGGGGTATCTCCTTGACTCCAGTTACCACTAGAACTTGCGATGCGCTGG

Insertion site

Left flank	Direct repeat	Right flank	DR Length
TATCCCGGTA	TCCGTGTA	TTATCCCACA	8

DNA sequence

TAGGGGTATCTCCTTGACTCCAGTACCCCCAAGATATAGCGTACCGGGCCATGGAGGAGTACCCGCGGACCCTGGCCGAACTCGAACGACGCTTCGCGAC
GGATGCCGCCTGCCGAGCGTACCTGGTCCGGCTCCGGTGGCCCGACGGCTTTGTCTGCCCCCGGTGCGGGGGGCGCACGGCATGGGAGACGCAGCGCGGC
GTGTGGGTCTGTGCCGCCTGCGGGCACCAAGCCTCGGTGACGGCCGGGACGATCTTCCAGGACACGCGCTCGCCCTTGACGCTCTGGTTCCGCGCCATCT
GGTGGGTGACCAGCCAGAAGACGGGGGCGAGCGCCCTGGGCCTGCAGCGCATCCTGGGGTTCGGGAGCTACGAGACGGCGTGGACGTGGCTGCACAAGCT
CCGCCGCGCGATGGTCCGCCCGGGCCGGGACCCGCTCGCGGGGCGCGTCGAGGTGGATGAGGCCTACCTCGGCGGCCCAGGGGCGGGCGCCCACGGCCGG
AAGCGGACGCCGAAGAAGATTCTGATCGCCGTGGCCGCCGAAGAAGCCGGCCGTGGGATCGGCCGGATCCGGGTACGCCGGATTCCGGACGCCACGGCAG
CCAGCCTGCACCGGTTCGTCCGCGAGGCCGTCGCGCCGGGCAGCGTCCTCCACACGGACGGCTGGGACGGCTACACGGGGCTGGGCGCGCAGGGCTATAC
GCATGAGGTCAGTCCGCTCCGCCGCCGCCCGGGGGCGGCCGTCGCCCTGTTGCCCCGGGTCCACCGGGTGGTCGCGCTCCTGAAGCGGTGGTTGCTGGGG
ACCCACCAGGGGGCGGTGAGTGTGGCGCATCTCGACTACTACCTGGACGAATTCACGTTCCGGTTCAACCGGCGGACGTCCCGCTCCCGTGGGAAGCTCT
TCTACCGCGTGCTCCAGCACGCCGTGGTCGTGGACCCGGTCCCGTACACGGCCCTGGTGACGCACACGCGTGGCCGGCCGCCAGCGCATCGCAAGTTCTA
GTGGTAACTGGAGTCAAGGAGATACCCCTA

Protein section

ORF number : 1

ORF 1

Length		Begin	End	Strand	Fusion ORF
951 bp	316 aa	51	1001	+	No

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MEEYPRTLAELERRFATDAACRAYLVRLRWPDGFVCPRCGGRTAWETQRGVWVCAACGHQASVTAGTIFQDTRSPLTLWFRAIWWVTSQKTGASALGLQR
ILGFGSYETAWTWLHKLRRAMVRPGRDPLAGRVEVDEAYLGGPGAGAHGRKRTPKKILIAVAAEEAGRGIGRIRVRRIPDATAASLHRFVREAVAPGSVL
HTDGWDGYTGLGAQGYTHEVSPLRRRPGAAVALLPRVHRVVALLKRWLLGTHQGAVSVAHLDYYLDEFTFRFNRRTSRSRGKLFYRVLQHAVVVDPVPYT
ALVTHTRGRPPAHRKF

Blast result :

Comments

ISUnb3 is 71% aa similar to ISDal1.

References

1] ISfinder annotation (2016).
2] Anantharaman,K., Brown,C.T., Hug,L.A., Sharon,I., Castelle,C.J., Probst,A.J., Thomas,B.C., Singh,A., Wilkins,M.J., Karaoz,U., Brodie,E.L., Williams,K.H., Hubbard,S.S. and Banfield,J.F. (2016) Nat Commun 7, 13219.